LOCUS Exported 7328 bp ds-DNA circular SYN 19-JUN-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS A7 Tol2-lyz-EGFP-L10a SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7328) AUTHORS . TITLE Direct Submission JOURNAL Exported Wednesday, Jun 19, 2019 from SnapGene Viewer 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7328 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 443..464 /note="Tol2-Rarm-443-Seq" misc_feature 561..724 /note="Tol2-right arm" misc_feature 561..710 /note="Tol2 mini R arm" misc_feature complement(698..730) /note="Tol2-Rarm-NHE-I" misc_feature 744..3112 /note="lyz promoter" misc_feature 3128..3870 /label=EGFP misc_feature 3884..4531 /label=L10a misc_feature 4534 /label=Untitled misc_feature 4676..4726 /note="SV40 Poly A" misc_feature 4866..4895 /note="Tol2-Larn-NotI" misc_feature complement(4874..5089) /note="Tol2-left arm" misc_feature 4890..5089 /note="Tol2 mini L arm" misc_feature complement(5113..5133) /note="Tol2-Larm-R-XbaI" misc_feature 5131..5153 /note="Tol2-Larm-F-XbaI" misc_feature complement(5209..5231) /note="Tol2-Larm-1130-Seq" misc_feature 6344..7204 /note="Ampr" ORIGIN 1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 301 ggagcccccg atttagagct tgacggggaa agccggccac aatgacggct actacatggt 361 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaagg ctcttggata 421 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct 481 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag 541 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa 601 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct 661 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa 721 aaggctagca agatctgctc gagagtttga cttgggttga ttatagcaaa tagtggaaca 781 caaacagatt ttgaaatatt cttgcaaatt tatttattaa atccattcaa atattgtcat 841 tttttgtact gtgtaattta tattgatttg ctcatacaat atttacattt cgcttaaaca 901 atacactatt atttatattt aaaaatagct atatataaat caaatgtata gctctttttt 961 acacaattaa tcaaagctaa aatatttgtc acacaaatac agtaagccac ttcacaatga 1021 tatcttcaaa atctgtgctt gactgaatca tttcatttat tgttttgtaa ttcacattat 1081 accttacgtt ctccaggcac agtttgaatt attgataaat gaagataata catctgattt 1141 aattagtctc ctttgtttat aataacttta atctaatttg atagtaataa ttccagcaat 1201 acatccatta caataaaaat cgctttttaa ttttgcacat tagtttgccg gtatttgttt 1261 aaagtttcac catactttta aatcatgtgc tatttgaaaa gggctgttat gactggaatt 1321 aaatgttttt ttttttatat cagtgtttta gattgaagta caatatttat aggtaaaatt 1381 caccagcata tgcttaaaga tagccatgct ccaggtcctt gtgtccttct aaggtctgtt 1441 tgtggtttaa gctgtttgtt gacacacgct tgtcttctcc atttttctgt gcagttattg 1501 tcagtttatt gcacactggg ttgcattaag ctgcagtttt ttcacaacag ttttccacat 1561 tgtaacagtc agtctccaaa ttattccatc agtcctcaaa tttagtcatt tgaggccatg 1621 aaaacactta aaactacata aaggtagttt ggcattaggt tttagacttg ttcttgtttg 1681 gcaatattgc aacctgtgta aaataatttg attctttttt tttttttttt ttttttaatt 1741 ttcagaaaga aatggtccaa aaggagctat gtgaacattt atccatgtgt ttaatgtttt 1801 tttttttatt attaaaccac ttctgactta acttttttta gttattctat tttgtgacga 1861 gtctgaatgg ccaaagtact tttgaatcca aaatcttact agtaaaatgt taaatcttac 1921 tagcagtgtt gggtataatt tgtaacaaag ttaaattcca tatctgctaa gtgaaaaagt 1981 aaagtaagag attgctgtaa atatttaggt aatttgtttt attaatacaa tatagaaagt 2041 aacaaaacac aatgtaaaaa aatcaataaa gaaagtaaat aataaaagta atgataaact 2101 aatgatctaa acaaaattag taataaaatt tatacaattc agggagggta aagaatttca 2161 cctaatagtc aaactctgta ttaaataata taaaatattt gttcttagtc tgaagaggag 2221 actgtgagag aaagcttact ggttattaaa ttagaaaatt tgtaacttgc agattaaagc 2281 tgtgtgcctt gcagattaaa ataatttaaa taatttaata aaggccatgt ttgagacaaa 2341 tgtaaaaaca taataaatgt taagaatttc agatattgag attttacaat ttaaaaaaag 2401 caataaagta atagcagaga cctacactct taaagtaaat tactcaacac tgcttactag 2461 taacttttgg aatagttact agtatccatt gaacaaatac tagaatctaa ttaaattgtg 2521 tgagtgtcca atgaaaagat tcttgtattt aaaacagtac cagtatctat atatgtacaa 2581 aatttagtgc tagcaaaatg caaataaata ctactgcata taatggattg cattgtaaat 2641 cgacttgcca taatacattt atttcctatt tttactttta acaaaaaata agttcactga 2701 gtatttcatg tcattaaatc gcattaaacc tcttgcatgc tttatgtaca ctagtgcagt 2761 tcgctaaatg accaccggtg tcactaataa cacgatcgtt tctgaattct gcattaaacg 2821 ccatttatac gttgctctta ttagtaaaca atttctccat catgagctcg accagtattg 2881 catatttcat cctcttaaat ccagagttgt ttcttttaat cactttctct tcatgataaa 2941 agcagttttt ggttttcaga agtttacaac aaacccagtc agttattgcc tcagcaaaca 3001 ctggagcaac tgcacacttg tgaaaagctt cttgcgacac aaacctttct ctggacttat 3061 aaataaaggc attcagcagc tccaccatac acctccactg gtcttgataa agggtacccc 3121 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 3181 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 3241 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 3301 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 3361 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 3421 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 3481 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 3541 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 3601 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 3661 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 3721 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 3781 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 3841 caagtccggc cggactcaga tctcgagctc aagcttcgaa ttcagcagca aagtctcacg 3901 cgacaccctg tacgaggcgg tgcgggaagt cctgcacggg aaccagcgca agcgccgcaa 3961 gtttctggag acggtggagc tgcagatcag cctgaagaac tacgaccctc agaaggacaa 4021 acgtttctcg ggcaccgtca ggctcaagtc caccccacgc cccaagttct cggtgtgcgt 4081 tctgggggac cagcagcact gtgatgaagc caaggccgtg gatatccccc acatggacat 4141 cgaggcgctc aagaagctta acaaaaacaa gaagttggtc aagaagctgg ctaagaagta 4201 cgatgccttt ttggcctctg agtctctgat taagcagatc ccacgtatcc tgggcccagg 4261 cctaaacaag gctggcaagt tcccctccct gctgacacac aatgaaaaca tggtggccaa 4321 agtggatgag gtgaaatcga caatcaagtt ccagatgaag aaggtgctgt gtttggccgt 4381 cgctgttggc cacgtgaaga tgaccgatga tgagctagtc tacaacattc atctggctgt 4441 caatttcttg gtgtccttgc ttaagaaaaa ctggcaaaac gtgcgggctc tgtacatcaa 4501 gagcaccatg ggcaagcccc agcgtctgta ttagtctaga tcataatcag ccataccaca 4561 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 4621 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 4681 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 4741 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa 4801 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcgg 4861 atcctgcggc cgcaagggaa aatagaatga agtgatctcc aaaaaataag tactttttga 4921 ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt 4981 aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat 5041 acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg ttcttgaccc 5101 cctaccttca gcaagcccag cagatccact agatcttttg ttccctttag tgagggttaa 5161 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 5221 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 5281 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 5341 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 5401 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 5461 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 5521 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 5581 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 5641 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 5701 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 5761 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 5821 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 5881 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 5941 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 6001 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 6061 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 6121 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 6181 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 6241 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 6301 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 6361 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 6421 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 6481 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 6541 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 6601 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 6661 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 6721 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 6781 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 6841 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 6901 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 6961 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 7021 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 7081 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 7141 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 7201 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 7261 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 7321 gaaaagtg //