LOCUS       Exported                7328 bp ds-DNA     circular SYN 19-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    A7 Tol2-lyz-EGFP-L10a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 19, 2019 from SnapGene Viewer 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    443..464
                     /note="Tol2-Rarm-443-Seq"
     misc_feature    561..724
                     /note="Tol2-right arm"
     misc_feature    561..710
                     /note="Tol2 mini R arm"
     misc_feature    complement(698..730)
                     /note="Tol2-Rarm-NHE-I"
     misc_feature    744..3112
                     /note="lyz promoter"
     misc_feature    3128..3870
                     /label=EGFP
     misc_feature    3884..4531
                     /label=L10a
     misc_feature    4534
                     /label=Untitled
     misc_feature    4676..4726
                     /note="SV40 Poly A"
     misc_feature    4866..4895
                     /note="Tol2-Larn-NotI"
     misc_feature    complement(4874..5089)
                     /note="Tol2-left arm"
     misc_feature    4890..5089
                     /note="Tol2 mini L arm"
     misc_feature    complement(5113..5133)
                     /note="Tol2-Larm-R-XbaI"
     misc_feature    5131..5153
                     /note="Tol2-Larm-F-XbaI"
     misc_feature    complement(5209..5231)
                     /note="Tol2-Larm-1130-Seq"
     misc_feature    6344..7204
                     /note="Ampr"
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggccac aatgacggct actacatggt
      361 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaagg ctcttggata
      421 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct
      481 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag
      541 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa
      601 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct
      661 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa
      721 aaggctagca agatctgctc gagagtttga cttgggttga ttatagcaaa tagtggaaca
      781 caaacagatt ttgaaatatt cttgcaaatt tatttattaa atccattcaa atattgtcat
      841 tttttgtact gtgtaattta tattgatttg ctcatacaat atttacattt cgcttaaaca
      901 atacactatt atttatattt aaaaatagct atatataaat caaatgtata gctctttttt
      961 acacaattaa tcaaagctaa aatatttgtc acacaaatac agtaagccac ttcacaatga
     1021 tatcttcaaa atctgtgctt gactgaatca tttcatttat tgttttgtaa ttcacattat
     1081 accttacgtt ctccaggcac agtttgaatt attgataaat gaagataata catctgattt
     1141 aattagtctc ctttgtttat aataacttta atctaatttg atagtaataa ttccagcaat
     1201 acatccatta caataaaaat cgctttttaa ttttgcacat tagtttgccg gtatttgttt
     1261 aaagtttcac catactttta aatcatgtgc tatttgaaaa gggctgttat gactggaatt
     1321 aaatgttttt ttttttatat cagtgtttta gattgaagta caatatttat aggtaaaatt
     1381 caccagcata tgcttaaaga tagccatgct ccaggtcctt gtgtccttct aaggtctgtt
     1441 tgtggtttaa gctgtttgtt gacacacgct tgtcttctcc atttttctgt gcagttattg
     1501 tcagtttatt gcacactggg ttgcattaag ctgcagtttt ttcacaacag ttttccacat
     1561 tgtaacagtc agtctccaaa ttattccatc agtcctcaaa tttagtcatt tgaggccatg
     1621 aaaacactta aaactacata aaggtagttt ggcattaggt tttagacttg ttcttgtttg
     1681 gcaatattgc aacctgtgta aaataatttg attctttttt tttttttttt ttttttaatt
     1741 ttcagaaaga aatggtccaa aaggagctat gtgaacattt atccatgtgt ttaatgtttt
     1801 tttttttatt attaaaccac ttctgactta acttttttta gttattctat tttgtgacga
     1861 gtctgaatgg ccaaagtact tttgaatcca aaatcttact agtaaaatgt taaatcttac
     1921 tagcagtgtt gggtataatt tgtaacaaag ttaaattcca tatctgctaa gtgaaaaagt
     1981 aaagtaagag attgctgtaa atatttaggt aatttgtttt attaatacaa tatagaaagt
     2041 aacaaaacac aatgtaaaaa aatcaataaa gaaagtaaat aataaaagta atgataaact
     2101 aatgatctaa acaaaattag taataaaatt tatacaattc agggagggta aagaatttca
     2161 cctaatagtc aaactctgta ttaaataata taaaatattt gttcttagtc tgaagaggag
     2221 actgtgagag aaagcttact ggttattaaa ttagaaaatt tgtaacttgc agattaaagc
     2281 tgtgtgcctt gcagattaaa ataatttaaa taatttaata aaggccatgt ttgagacaaa
     2341 tgtaaaaaca taataaatgt taagaatttc agatattgag attttacaat ttaaaaaaag
     2401 caataaagta atagcagaga cctacactct taaagtaaat tactcaacac tgcttactag
     2461 taacttttgg aatagttact agtatccatt gaacaaatac tagaatctaa ttaaattgtg
     2521 tgagtgtcca atgaaaagat tcttgtattt aaaacagtac cagtatctat atatgtacaa
     2581 aatttagtgc tagcaaaatg caaataaata ctactgcata taatggattg cattgtaaat
     2641 cgacttgcca taatacattt atttcctatt tttactttta acaaaaaata agttcactga
     2701 gtatttcatg tcattaaatc gcattaaacc tcttgcatgc tttatgtaca ctagtgcagt
     2761 tcgctaaatg accaccggtg tcactaataa cacgatcgtt tctgaattct gcattaaacg
     2821 ccatttatac gttgctctta ttagtaaaca atttctccat catgagctcg accagtattg
     2881 catatttcat cctcttaaat ccagagttgt ttcttttaat cactttctct tcatgataaa
     2941 agcagttttt ggttttcaga agtttacaac aaacccagtc agttattgcc tcagcaaaca
     3001 ctggagcaac tgcacacttg tgaaaagctt cttgcgacac aaacctttct ctggacttat
     3061 aaataaaggc attcagcagc tccaccatac acctccactg gtcttgataa agggtacccc
     3121 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3181 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3241 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3301 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     3361 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3421 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3481 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3541 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3601 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3661 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3721 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3781 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3841 caagtccggc cggactcaga tctcgagctc aagcttcgaa ttcagcagca aagtctcacg
     3901 cgacaccctg tacgaggcgg tgcgggaagt cctgcacggg aaccagcgca agcgccgcaa
     3961 gtttctggag acggtggagc tgcagatcag cctgaagaac tacgaccctc agaaggacaa
     4021 acgtttctcg ggcaccgtca ggctcaagtc caccccacgc cccaagttct cggtgtgcgt
     4081 tctgggggac cagcagcact gtgatgaagc caaggccgtg gatatccccc acatggacat
     4141 cgaggcgctc aagaagctta acaaaaacaa gaagttggtc aagaagctgg ctaagaagta
     4201 cgatgccttt ttggcctctg agtctctgat taagcagatc ccacgtatcc tgggcccagg
     4261 cctaaacaag gctggcaagt tcccctccct gctgacacac aatgaaaaca tggtggccaa
     4321 agtggatgag gtgaaatcga caatcaagtt ccagatgaag aaggtgctgt gtttggccgt
     4381 cgctgttggc cacgtgaaga tgaccgatga tgagctagtc tacaacattc atctggctgt
     4441 caatttcttg gtgtccttgc ttaagaaaaa ctggcaaaac gtgcgggctc tgtacatcaa
     4501 gagcaccatg ggcaagcccc agcgtctgta ttagtctaga tcataatcag ccataccaca
     4561 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     4621 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     4681 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4741 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
     4801 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcgg
     4861 atcctgcggc cgcaagggaa aatagaatga agtgatctcc aaaaaataag tactttttga
     4921 ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt
     4981 aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat
     5041 acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg ttcttgaccc
     5101 cctaccttca gcaagcccag cagatccact agatcttttg ttccctttag tgagggttaa
     5161 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5221 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5281 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5341 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     5401 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5461 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5521 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5581 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5641 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5701 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5761 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5821 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5881 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5941 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6001 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6061 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6121 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6181 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6241 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6301 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6361 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6421 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6481 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6541 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6601 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6661 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6721 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6781 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6841 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6901 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6961 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7021 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7081 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7141 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7201 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7261 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7321 gaaaagtg
//
