LOCUS       pJ211_WT_hi             4076 bp    DNA     circular UNA 18-NOV-2019
DEFINITION  Gibson Assembly ligation product.
ACCESSION   urn.local...p-arolxct
VERSION     urn.local...p-arolxct
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    complement(580..1389)
                     /product="M_Kanamycin-r_*"
                     /note="Geneious type: gene"
                     /standard_name="M_Kanamycin-r_*"
     misc_feature    complement(1400..1517)
                     /product="P_Amp"
                     /note="Geneious type: promoter"
                     /standard_name="P_Amp"
     misc_feature    1772..2009
                     /standard_name="fliC Operator/promoter cassette"
     promoter        1909..1935
     regulatory      1985..1990
                     /standard_name="potential RBS"
     gene            2012..3508
                     /standard_name="Flagellin"
     misc_feature    complement(3575..3875)
                     /product="Term_bla"
                     /note="Geneious type: terminator"
                     /standard_name="Term_bla"
     misc_feature    complement(3876..3995)
                     /product="Term_rpoC"
                     /note="Geneious type: terminator"
                     /standard_name="Term_rpoC"
     rep_origin      complement(4063..575)
ORIGIN      
        1 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
       61 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      121 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      181 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      241 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      301 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      361 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      421 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      481 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      541 agcagcagat tacgcgcaga aaaaaaggat ctcaagcttt tagaaaaact catcgagcat
      601 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      661 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      721 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      781 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      841 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      901 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac
      961 gcgatcgctg ttaaaaggac aattacaaac aggaatcgag tgcaaccggc gcaggaacac
     1021 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaacgc
     1081 tgtttttccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1141 cttgatggtc ggaagtggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1201 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1261 cccatacaag cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1321 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgacgttt cccgttgaat
     1381 atggctcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1441 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca gtgttacaac
     1501 caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg gctcataaca
     1561 ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag
     1621 aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga gtagggaact
     1681 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg cccgggctaa
     1741 ttagggggtg tcgcccttcg ctgaagtcga cgtaaaacga ataccggggt tatcggtctg
     1801 aattgcgcaa agtttacgtt taattgtttt ttttaatagc gggaataagg ggcagagaaa
     1861 agagtatttc ggcgactaac aaaaaatggc tgtttttgaa aaaaattcta aaggttgttt
     1921 tacgacagac gataacaggg ttgacggcga ttgagccgac gggtggaaac ccaatacgta
     1981 atcaacgact tgcaatatag gataacgaac catggcacaa gtcattaata ccaacagcct
     2041 ctcgctgatc actcaaaata atatcaacaa gaaccagtct gcgctgtcga gttctatcga
     2101 gcgtctgtct tctggcttgc gtattaacag cgcgaaggat gacgcagcgg gtcaggcgat
     2161 tgctaaccgt ttcacctcta acattaaagg cctgactcag gcggcccgta acgccaacga
     2221 cggtatctcc gttgcgcaga ccaccgaagg cgcgctgtcc gaaatcaaca acaacttaca
     2281 gcgtgtgcgt gaactgacgg tacaggccac taccggtact aactctgagt ctgatctgtc
     2341 ttctatccag gacgaaatta aatcccgtct ggatgaaatt gaccgcgtat ctggtcagac
     2401 ccagttcaac ggcgtgaacg tgctggcaaa aaatggctcc atgaaaatcc aggttggcgc
     2461 aaatgataac cagactatca ctatcgatct gaagcagatt gatgctaaaa ctcttggcct
     2521 tgatggtttt agcgttaaaa ataacgatac agttaccact agtgctccag taactgcttt
     2581 tggtgctacc accacaaaca atattaaact tactggaatt accctttcta cggaagcagc
     2641 cactgatact ggcggaacta acccagcttc aattgagggt gtttatactg ataatggtaa
     2701 tgattactat gcgaaaatca ccggtggtga taacgatggg aagtattacg cagtaacagt
     2761 tgctaatgat ggtacagtga caatggcgac tggagcaacg gcaaatgcaa ctgtaactga
     2821 tgcaaatact actaaagcta caactatcac ttcaggcggt acacctgttc agattgataa
     2881 tactgcaggt tccgcaactg ccaaccttgg tgctgttagc ttagtaaaac tgcaggattc
     2941 caagggtaat gataccgata catatgcgct taaagataca aatggcaatc tttacgctgc
     3001 ggatgtgaat gaaactactg gtgctgtttc tgttaaaact attacctata ctgactcttc
     3061 cggtgccgcc agttctccaa ccgcggtcaa actgggcgga gatgatggca aaacagaagt
     3121 ggtcgatatt gatggtaaaa catacgattc tgccgattta aatggcggta atctgcaaac
     3181 aggtttgact gctggtggtg aggctctgac tgctgttgca aatggtaaaa ccacggatcc
     3241 gctgaaagcg ctggacgatg ctatcgcatc tgtagacaaa ttccgttctt ccctcggtgc
     3301 ggtgcaaaac cgtctggatt ccgcggttac caacctgaac aacaccacta ccaacctgtc
     3361 tgaagcgcag tcccgtattc aggacgccga ctatgcgacc gaagtgtcca atatgtcgaa
     3421 agcgcagatc atccagcagg ccggtaactc cgtgttggca aaagctaacc aggtaccgca
     3481 gcaggttctg tctctgctgc agggttaaga attctgatca cactggctac ctttgggtgg
     3541 ccttttttta tcgtcaaaag ggcgacacaa aaggtattct aaatgcataa taaatactga
     3601 taacatctta tagtttgtat tatattttgt attatcgttg acatgtataa ttttgatatc
     3661 aaaaactgat tttcccttta ttattttcga gatttatttt cttaattctc tttaacaaac
     3721 tagaaatatt gtatatacaa aaaatcataa ataatagatg aatagtttaa ttataggtgt
     3781 tcatcaatcg aaaaagcaac gtatcttatt taaagtgcgt tgcttttttc tcatttataa
     3841 ggttaaataa ttctcatata tcaagcaaag tgacaggcgc ccttaaatat tctgacaaat
     3901 gctctttccc taaactcccc ccataaaaaa acccgccgaa gcgggttttt acgttatttg
     3961 cggattaacg attactcgtt atcagaaccg cccaggatgc ctggcagttc cctactctcg
     4021 ccgctgcgct cggtcgttcg gctgcgggac ctcagcgcta gctttccata ggctcc
//
