LOCUS       pJ211_rha_WT_low        5663 bp    DNA     circular UNA 18-NOV-2019
DEFINITION  Restriction Cloning ligation product.
ACCESSION   urn.local...l-arolxcs
VERSION     urn.local...l-arolxcs
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    20..35
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="RK2ori_Nhe_FOR"
     rep_origin      complement(34..565)
     CDS             958..2106
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /standard_name="trfA"
     terminator      complement(2169..2196)
     terminator      complement(2212..2283)
     primer_bind     complement(2272..2291)
                     /standard_name="RK2ori_Hin_REV"
     misc_feature    complement(2298..3107)
                     /product="M_Kanamycin-r_*"
                     /note="Geneious type: gene"
                     /standard_name="M_Kanamycin-r_*"
     misc_feature    complement(3118..3235)
                     /product="P_Amp"
                     /note="Geneious type: promoter"
                     /standard_name="P_Amp"
     promoter        3490..3608
     regulatory      3609..>3629
                     /standard_name="E. coli rhaBAD 5' UTR including RBS"
     regulatory      3618..3623
                     /standard_name="RBS"
     gene            3632..5128
                     /standard_name="Flagellin"
     misc_feature    complement(5195..5495)
                     /product="Term_bla"
                     /note="Geneious type: terminator"
                     /standard_name="Term_bla"
     misc_feature    complement(5496..5615)
                     /product="Term_rpoC"
                     /note="Geneious type: terminator"
                     /standard_name="Term_rpoC"
ORIGIN      
        1 gcgggacctc agcgctagcg cagagccatg tagggccgcc ggcgttgtgg atacctcgcg
       61 gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca
      121 cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg
      181 ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg
      241 acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga
      301 tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta
      361 ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg
      421 tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt
      481 gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc
      541 ccttctcgaa ccctcccggc ccgctaacgc gggcctccca tccccccagg ggctgcgccc
      601 ctcggccgcg aacggcctca ccccaaaaat ggcagccacg tagaaagcca gtccgcagaa
      661 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      721 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      781 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      841 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      901 aagatcgacg gatcgatccg gggaattaat tccggggcaa tcccgcaagg agggtgaatg
      961 aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg gttttccgcc
     1021 gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga aaccttccag
     1081 tccgtcggct cgatagtcca gcaagctacg gccaagatcg agcgcgacag cgtgcaactg
     1141 gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg tctcgaacag
     1201 gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat gacgaccaag
     1261 aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaagccaa gcaggccgcg
     1321 ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt cgatattgcg
     1381 ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc cctgttcacc
     1441 acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt ccacgtcaac
     1501 aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga cgaactggtg
     1561 tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat caccttcacg
     1621 ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta cacgaaggcc
     1681 gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga ccgcgttggg
     1741 cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg caagaaaacg
     1801 tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg cgaccactac
     1861 acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg gatgttcgac
     1921 tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg cctcatgtgc
     1981 ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc ctgcgaagag
     2041 ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt gcattgcaaa
     2101 cgctagggcc ttgtggggtc agttccggct gggggttcag cagccacctg catcgcccta
     2161 ggtataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt
     2221 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     2281 ttgatgcctg gaagctttta gaaaaactca tcgagcatca aatgaaactg caatttattc
     2341 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     2401 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     2461 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     2521 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag
     2581 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     2641 ttattcattc gtgattgcgc ctgagcgagg cgaaatacgc gatcgctgtt aaaaggacaa
     2701 ttacaaacag gaatcgagtg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     2761 tcacctgaat caggatattc ttctaatacc tggaacgctg tttttccggg gatcgcagtg
     2821 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagtggcata
     2881 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     2941 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaagcg atagattgtc
     3001 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     3061 ttggaattta atcgcggcct cgacgtttcc cgttgaatat ggctcatatt cttccttttt
     3121 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3181 atttagaaaa ataaacaaat aggggtcagt gttacaacca attaaccaat tctgaacatt
     3241 atcgcgagcc catttatacc tgaatatggc tcataacacc ccttgtttgc ctggcggcag
     3301 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
     3361 tggtagtgtg gggactcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa
     3421 aggctcagtc gaaagactgg gcctttcgcc cgggctaatt agggggtgtc gcccttcgct
     3481 gaagtcgacc accacaattc agcaaattgt gaacatcatc acgttcatct ttccctggtt
     3541 gccaatggcc cattttcctg tcagtaacga gaaggtcgcg tattcaggcg ctttttagac
     3601 tggtcgtaat gaaattcagc aggatcacac catggcacaa gtcattaata ccaacagcct
     3661 ctcgctgatc actcaaaata atatcaacaa gaaccagtct gcgctgtcga gttctatcga
     3721 gcgtctgtct tctggcttgc gtattaacag cgcgaaggat gacgcagcgg gtcaggcgat
     3781 tgctaaccgt ttcacctcta acattaaagg cctgactcag gcggcccgta acgccaacga
     3841 cggtatctcc gttgcgcaga ccaccgaagg cgcgctgtcc gaaatcaaca acaacttaca
     3901 gcgtgtgcgt gaactgacgg tacaggccac taccggtact aactctgagt ctgatctgtc
     3961 ttctatccag gacgaaatta aatcccgtct ggatgaaatt gaccgcgtat ctggtcagac
     4021 ccagttcaac ggcgtgaacg tgctggcaaa aaatggctcc atgaaaatcc aggttggcgc
     4081 aaatgataac cagactatca ctatcgatct gaagcagatt gatgctaaaa ctcttggcct
     4141 tgatggtttt agcgttaaaa ataacgatac agttaccact agtgctccag taactgcttt
     4201 tggtgctacc accacaaaca atattaaact tactggaatt accctttcta cggaagcagc
     4261 cactgatact ggcggaacta acccagcttc aattgagggt gtttatactg ataatggtaa
     4321 tgattactat gcgaaaatca ccggtggtga taacgatggg aagtattacg cagtaacagt
     4381 tgctaatgat ggtacagtga caatggcgac tggagcaacg gcaaatgcaa ctgtaactga
     4441 tgcaaatact actaaagcta caactatcac ttcaggcggt acacctgttc agattgataa
     4501 tactgcaggt tccgcaactg ccaaccttgg tgctgttagc ttagtaaaac tgcaggattc
     4561 caagggtaat gataccgata catatgcgct taaagataca aatggcaatc tttacgctgc
     4621 ggatgtgaat gaaactactg gtgctgtttc tgttaaaact attacctata ctgactcttc
     4681 cggtgccgcc agttctccaa ccgcggtcaa actgggcgga gatgatggca aaacagaagt
     4741 ggtcgatatt gatggtaaaa catacgattc tgccgattta aatggcggta atctgcaaac
     4801 aggtttgact gctggtggtg aggctctgac tgctgttgca aatggtaaaa ccacggatcc
     4861 gctgaaagcg ctggacgatg ctatcgcatc tgtagacaaa ttccgttctt ccctcggtgc
     4921 ggtgcaaaac cgtctggatt ccgcggttac caacctgaac aacaccacta ccaacctgtc
     4981 tgaagcgcag tcccgtattc aggacgccga ctatgcgacc gaagtgtcca atatgtcgaa
     5041 agcgcagatc atccagcagg ccggtaactc cgtgttggca aaagctaacc aggtaccgca
     5101 gcaggttctg tctctgctgc agggttaaga attctgatca cactggctac ctttgggtgg
     5161 ccttttttta tcgtcaaaag ggcgacacaa aaggtattct aaatgcataa taaatactga
     5221 taacatctta tagtttgtat tatattttgt attatcgttg acatgtataa ttttgatatc
     5281 aaaaactgat tttcccttta ttattttcga gatttatttt cttaattctc tttaacaaac
     5341 tagaaatatt gtatatacaa aaaatcataa ataatagatg aatagtttaa ttataggtgt
     5401 tcatcaatcg aaaaagcaac gtatcttatt taaagtgcgt tgcttttttc tcatttataa
     5461 ggttaaataa ttctcatata tcaagcaaag tgacaggcgc ccttaaatat tctgacaaat
     5521 gctctttccc taaactcccc ccataaaaaa acccgccgaa gcgggttttt acgttatttg
     5581 cggattaacg attactcgtt atcagaaccg cccaggatgc ctggcagttc cctactctcg
     5641 ccgctgcgct cggtcgttcg gct
//
