LOCUS       pJ211_rha_WT_med        4211 bp    DNA     circular UNA 18-NOV-2019
DEFINITION  Restriction Cloning ligation product.
ACCESSION   urn.local...q-arolxct
VERSION     urn.local...q-arolxct
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     rep_origin      complement(15..841)
     misc_feature    complement(846..1655)
                     /product="M_Kanamycin-r_*"
                     /note="Geneious type: gene"
                     /standard_name="M_Kanamycin-r_*"
     misc_feature    complement(1666..1783)
                     /product="P_Amp"
                     /note="Geneious type: promoter"
                     /standard_name="P_Amp"
     promoter        2038..2156
     regulatory      2157..>2177
                     /standard_name="E. coli rhaBAD 5' UTR including RBS"
     regulatory      2166..2171
                     /standard_name="RBS"
     gene            2180..3676
                     /standard_name="Flagellin"
     misc_feature    complement(3743..4043)
                     /product="Term_bla"
                     /note="Geneious type: terminator"
                     /standard_name="Term_bla"
     misc_feature    complement(4044..4163)
                     /product="Term_rpoC"
                     /note="Geneious type: terminator"
                     /standard_name="Term_rpoC"
ORIGIN      
        1 gcgggacctc agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg
       61 tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat
      121 gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
      181 gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat
      241 acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct
      301 gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa
      361 agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg
      421 tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag
      481 ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga
      541 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc
      601 accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg
      661 gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc
      721 ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt
      781 cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta
      841 agcttttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
      901 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
      961 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     1021 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     1081 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
     1141 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     1201 gattgcgcct gagcgaggcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     1261 atcgagtgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     1321 ggatattctt ctaatacctg gaacgctgtt tttccgggga tcgcagtggt gagtaaccat
     1381 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gtggcataaa ttccgtcagc
     1441 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     1501 agaaacaact ctggcgcatc gggcttccca tacaagcgat agattgtcgc acctgattgc
     1561 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     1621 cgcggcctcg acgtttcccg ttgaatatgg ctcatattct tcctttttca atattattga
     1681 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1741 aaacaaatag gggtcagtgt tacaaccaat taaccaattc tgaacattat cgcgagccca
     1801 tttatacctg aatatggctc ataacacccc ttgtttgcct ggcggcagta gcgcggtggt
     1861 cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg
     1921 gactccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga
     1981 aagactgggc ctttcgcccg ggctaattag ggggtgtcgc ccttcgctga agtcgaccac
     2041 cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca
     2101 ttttcctgtc agtaacgaga aggtcgcgta ttcaggcgct ttttagactg gtcgtaatga
     2161 aattcagcag gatcacacca tggcacaagt cattaatacc aacagcctct cgctgatcac
     2221 tcaaaataat atcaacaaga accagtctgc gctgtcgagt tctatcgagc gtctgtcttc
     2281 tggcttgcgt attaacagcg cgaaggatga cgcagcgggt caggcgattg ctaaccgttt
     2341 cacctctaac attaaaggcc tgactcaggc ggcccgtaac gccaacgacg gtatctccgt
     2401 tgcgcagacc accgaaggcg cgctgtccga aatcaacaac aacttacagc gtgtgcgtga
     2461 actgacggta caggccacta ccggtactaa ctctgagtct gatctgtctt ctatccagga
     2521 cgaaattaaa tcccgtctgg atgaaattga ccgcgtatct ggtcagaccc agttcaacgg
     2581 cgtgaacgtg ctggcaaaaa atggctccat gaaaatccag gttggcgcaa atgataacca
     2641 gactatcact atcgatctga agcagattga tgctaaaact cttggccttg atggttttag
     2701 cgttaaaaat aacgatacag ttaccactag tgctccagta actgcttttg gtgctaccac
     2761 cacaaacaat attaaactta ctggaattac cctttctacg gaagcagcca ctgatactgg
     2821 cggaactaac ccagcttcaa ttgagggtgt ttatactgat aatggtaatg attactatgc
     2881 gaaaatcacc ggtggtgata acgatgggaa gtattacgca gtaacagttg ctaatgatgg
     2941 tacagtgaca atggcgactg gagcaacggc aaatgcaact gtaactgatg caaatactac
     3001 taaagctaca actatcactt caggcggtac acctgttcag attgataata ctgcaggttc
     3061 cgcaactgcc aaccttggtg ctgttagctt agtaaaactg caggattcca agggtaatga
     3121 taccgataca tatgcgctta aagatacaaa tggcaatctt tacgctgcgg atgtgaatga
     3181 aactactggt gctgtttctg ttaaaactat tacctatact gactcttccg gtgccgccag
     3241 ttctccaacc gcggtcaaac tgggcggaga tgatggcaaa acagaagtgg tcgatattga
     3301 tggtaaaaca tacgattctg ccgatttaaa tggcggtaat ctgcaaacag gtttgactgc
     3361 tggtggtgag gctctgactg ctgttgcaaa tggtaaaacc acggatccgc tgaaagcgct
     3421 ggacgatgct atcgcatctg tagacaaatt ccgttcttcc ctcggtgcgg tgcaaaaccg
     3481 tctggattcc gcggttacca acctgaacaa caccactacc aacctgtctg aagcgcagtc
     3541 ccgtattcag gacgccgact atgcgaccga agtgtccaat atgtcgaaag cgcagatcat
     3601 ccagcaggcc ggtaactccg tgttggcaaa agctaaccag gtaccgcagc aggttctgtc
     3661 tctgctgcag ggttaagaat tctgatcaca ctggctacct ttgggtggcc tttttttatc
     3721 gtcaaaaggg cgacacaaaa ggtattctaa atgcataata aatactgata acatcttata
     3781 gtttgtatta tattttgtat tatcgttgac atgtataatt ttgatatcaa aaactgattt
     3841 tccctttatt attttcgaga tttattttct taattctctt taacaaacta gaaatattgt
     3901 atatacaaaa aatcataaat aatagatgaa tagtttaatt ataggtgttc atcaatcgaa
     3961 aaagcaacgt atcttattta aagtgcgttg cttttttctc atttataagg ttaaataatt
     4021 ctcatatatc aagcaaagtg acaggcgccc ttaaatattc tgacaaatgc tctttcccta
     4081 aactcccccc ataaaaaaac ccgccgaagc gggtttttac gttatttgcg gattaacgat
     4141 tactcgttat cagaaccgcc caggatgcct ggcagttccc tactctcgcc gctgcgctcg
     4201 gtcgttcggc t
//
