LOCUS       pJ211_rha_WT_hi         3978 bp    DNA     circular UNA 18-NOV-2019
DEFINITION  Restriction Cloning ligation product.
ACCESSION   urn.local...j-arolxcs
VERSION     urn.local...j-arolxcs
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     rep_origin      complement(20..608)
     misc_feature    complement(613..1422)
                     /product="M_Kanamycin-r_*"
                     /note="Geneious type: gene"
                     /standard_name="M_Kanamycin-r_*"
     misc_feature    complement(1433..1550)
                     /product="P_Amp"
                     /note="Geneious type: promoter"
                     /standard_name="P_Amp"
     promoter        1805..1923
     regulatory      1924..>1944
                     /standard_name="E. coli rhaBAD 5' UTR including RBS"
     regulatory      1933..1938
                     /standard_name="RBS"
     gene            1947..3443
                     /standard_name="Flagellin"
     misc_feature    complement(3510..3810)
                     /product="Term_bla"
                     /note="Geneious type: terminator"
                     /standard_name="Term_bla"
     misc_feature    complement(3811..3930)
                     /product="Term_rpoC"
                     /note="Geneious type: terminator"
                     /standard_name="Term_rpoC"
ORIGIN      
        1 gcgggacctc agcgctagct ttccataggc tccgcccccc tgacgagcat cacaaaaatc
       61 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      121 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      181 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      241 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      301 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      361 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      421 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      481 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      541 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      601 gatctcaagc ttttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
      661 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
      721 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
      781 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
      841 atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg
      901 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
      961 cattcgtgat tgcgcctgag cgaggcgaaa tacgcgatcg ctgttaaaag gacaattaca
     1021 aacaggaatc gagtgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     1081 tgaatcagga tattcttcta atacctggaa cgctgttttt ccggggatcg cagtggtgag
     1141 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagtg gcataaattc
     1201 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     1261 atgtttcaga aacaactctg gcgcatcggg cttcccatac aagcgataga ttgtcgcacc
     1321 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     1381 atttaatcgc ggcctcgacg tttcccgttg aatatggctc atattcttcc tttttcaata
     1441 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1501 gaaaaataaa caaatagggg tcagtgttac aaccaattaa ccaattctga acattatcgc
     1561 gagcccattt atacctgaat atggctcata acaccccttg tttgcctggc ggcagtagcg
     1621 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
     1681 gtgtggggac tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     1741 cagtcgaaag actgggcctt tcgcccgggc taattagggg gtgtcgccct tcgctgaagt
     1801 cgaccaccac aattcagcaa attgtgaaca tcatcacgtt catctttccc tggttgccaa
     1861 tggcccattt tcctgtcagt aacgagaagg tcgcgtattc aggcgctttt tagactggtc
     1921 gtaatgaaat tcagcaggat cacaccatgg cacaagtcat taataccaac agcctctcgc
     1981 tgatcactca aaataatatc aacaagaacc agtctgcgct gtcgagttct atcgagcgtc
     2041 tgtcttctgg cttgcgtatt aacagcgcga aggatgacgc agcgggtcag gcgattgcta
     2101 accgtttcac ctctaacatt aaaggcctga ctcaggcggc ccgtaacgcc aacgacggta
     2161 tctccgttgc gcagaccacc gaaggcgcgc tgtccgaaat caacaacaac ttacagcgtg
     2221 tgcgtgaact gacggtacag gccactaccg gtactaactc tgagtctgat ctgtcttcta
     2281 tccaggacga aattaaatcc cgtctggatg aaattgaccg cgtatctggt cagacccagt
     2341 tcaacggcgt gaacgtgctg gcaaaaaatg gctccatgaa aatccaggtt ggcgcaaatg
     2401 ataaccagac tatcactatc gatctgaagc agattgatgc taaaactctt ggccttgatg
     2461 gttttagcgt taaaaataac gatacagtta ccactagtgc tccagtaact gcttttggtg
     2521 ctaccaccac aaacaatatt aaacttactg gaattaccct ttctacggaa gcagccactg
     2581 atactggcgg aactaaccca gcttcaattg agggtgttta tactgataat ggtaatgatt
     2641 actatgcgaa aatcaccggt ggtgataacg atgggaagta ttacgcagta acagttgcta
     2701 atgatggtac agtgacaatg gcgactggag caacggcaaa tgcaactgta actgatgcaa
     2761 atactactaa agctacaact atcacttcag gcggtacacc tgttcagatt gataatactg
     2821 caggttccgc aactgccaac cttggtgctg ttagcttagt aaaactgcag gattccaagg
     2881 gtaatgatac cgatacatat gcgcttaaag atacaaatgg caatctttac gctgcggatg
     2941 tgaatgaaac tactggtgct gtttctgtta aaactattac ctatactgac tcttccggtg
     3001 ccgccagttc tccaaccgcg gtcaaactgg gcggagatga tggcaaaaca gaagtggtcg
     3061 atattgatgg taaaacatac gattctgccg atttaaatgg cggtaatctg caaacaggtt
     3121 tgactgctgg tggtgaggct ctgactgctg ttgcaaatgg taaaaccacg gatccgctga
     3181 aagcgctgga cgatgctatc gcatctgtag acaaattccg ttcttccctc ggtgcggtgc
     3241 aaaaccgtct ggattccgcg gttaccaacc tgaacaacac cactaccaac ctgtctgaag
     3301 cgcagtcccg tattcaggac gccgactatg cgaccgaagt gtccaatatg tcgaaagcgc
     3361 agatcatcca gcaggccggt aactccgtgt tggcaaaagc taaccaggta ccgcagcagg
     3421 ttctgtctct gctgcagggt taagaattct gatcacactg gctacctttg ggtggccttt
     3481 ttttatcgtc aaaagggcga cacaaaaggt attctaaatg cataataaat actgataaca
     3541 tcttatagtt tgtattatat tttgtattat cgttgacatg tataattttg atatcaaaaa
     3601 ctgattttcc ctttattatt ttcgagattt attttcttaa ttctctttaa caaactagaa
     3661 atattgtata tacaaaaaat cataaataat agatgaatag tttaattata ggtgttcatc
     3721 aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt tataaggtta
     3781 aataattctc atatatcaag caaagtgaca ggcgccctta aatattctga caaatgctct
     3841 ttccctaaac tccccccata aaaaaacccg ccgaagcggg tttttacgtt atttgcggat
     3901 taacgattac tcgttatcag aaccgcccag gatgcctggc agttccctac tctcgccgct
     3961 gcgctcggtc gttcggct
//
