LOCUS       pD441_WT_hi             5471 bp    DNA     circular UNA 18-NOV-2019
DEFINITION  Golden Gate/Type IIS Cloning ligation product.
ACCESSION   urn.local...3o-arom3oj
VERSION     urn.local...3o-arom3oj
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     rep_origin      27..615
     misc_feature    918..1037
                     /product="Term_rpoC"
                     /note="Geneious type: terminator"
                     /standard_name="Term_rpoC"
     misc_feature    1038..1338
                     /product="Term_bla"
                     /note="Geneious type: terminator"
                     /standard_name="Term_bla"
     misc_feature    1356..1473
                     /product="P_T5_Inducible"
                     /note="Geneious type: promoter"
                     /standard_name="P_T5_Inducible"
     misc_feature    1356..1376
                     /product="LacO1"
                     /note="Geneious type: regulatory"
                     /standard_name="LacO1"
     misc_feature    1444..1473
                     /product="lacO"
                     /note="Geneious type: regulatory"
                     /standard_name="lacO"
     misc_feature    complement(1444..1473)
                     /product="lacO"
                     /note="Geneious type: regulatory"
                     /standard_name="lacO"
     misc_feature    1474..1508
                     /product="5'UTR_T7_10A"
                     /note="Geneious type: regulatory"
                     /standard_name="5'UTR_T7_10A"
     misc_feature    1511..1522
                     /product="strong RBS"
                     /note="Geneious type: regulatory"
                     /standard_name="strong RBS"
     gene            1523..3019
                     /standard_name="Flagellin"
     misc_feature    3310..3427
                     /product="P_Amp"
                     /note="Geneious type: promoter"
                     /standard_name="P_Amp"
     misc_feature    3438..4247
                     /product="M_Kanamycin-r_*"
                     /note="Geneious type: gene"
                     /standard_name="M_Kanamycin-r_*"
     misc_feature    4248..4329
                     /product="P_lacI"
                     /standard_name="P_lacI"
     misc_feature    4330..5412
                     /product="M_lacI_*"
                     /note="Geneious type: gene"
                     /standard_name="M_lacI_*"
ORIGIN      
        1 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
       61 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
      181 gtgtagccgt agttagccca ccacttcaag aactctgtag caccgcctac atacctcgct
      241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      661 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
      721 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
      781 agcgaggaag cggaaggcga gagtagggaa ctgccaggca tcaaactaag cagaaggccc
      841 ctgacggatg gcctttttgc gtttctacaa actctttctg tgttgtaaaa cgacggccag
      901 tcttaagctc gggccccctg ggcggttctg ataacgagta atcgttaatc cgcaaataac
      961 gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt tagggaaaga gcatttgtca
     1021 gaatatttaa gggcgcctgt cactttgctt gatatatgag aattatttaa ccttataaat
     1081 gagaaaaaag caacgcactt taaataagat acgttgcttt ttcgattgat gaacacctat
     1141 aattaaacta ttcatctatt atttatgatt ttttgtatat acaatatttc tagtttgtta
     1201 aagagaatta agaaaataaa tctcgaaaat aataaaggga aaatcagttt ttgatatcaa
     1261 aattatacat gtcaacgata atacaaaata taatacaaac tataagatgt tatcagtatt
     1321 tattatgcat ttagaataaa ttttgtgtcg cccttaattg tgagcggata acaattacga
     1381 gcttcatgca cagtgaaatc atgaaaaatt tatttgcttt gtgagcggat aacaattata
     1441 atatgtggaa ttgtgagcgc tcacaattcc acaacggttt ccctctagaa ataattttgt
     1501 ttaactttta aggaggtaaa ccatggcaca agtcattaat accaacagcc tctcgctgat
     1561 cactcaaaat aatatcaaca agaaccagtc tgcgctgtcg agttctatcg agcgtctgtc
     1621 ttctggcttg cgtattaaca gcgcgaagga tgacgcagcg ggtcaggcga ttgctaaccg
     1681 tttcacctct aacattaaag gcctgactca ggcggcccgt aacgccaacg acggtatctc
     1741 cgttgcgcag accaccgaag gcgcgctgtc cgaaatcaac aacaacttac agcgtgtgcg
     1801 tgaactgacg gtacaggcca ctaccggtac taactctgag tctgatctgt cttctatcca
     1861 ggacgaaatt aaatcccgtc tggatgaaat tgaccgcgta tctggtcaga cccagttcaa
     1921 cggcgtgaac gtgctggcaa aaaatggctc catgaaaatc caggttggcg caaatgataa
     1981 ccagactatc actatcgatc tgaagcagat tgatgctaaa actcttggcc ttgatggttt
     2041 tagcgttaaa aataacgata cagttaccac tagtgctcca gtaactgctt ttggtgctac
     2101 caccacaaac aatattaaac ttactggaat taccctttct acggaagcag ccactgatac
     2161 tggcggaact aacccagctt caattgaggg tgtttatact gataatggta atgattacta
     2221 tgcgaaaatc accggtggtg ataacgatgg gaagtattac gcagtaacag ttgctaatga
     2281 tggtacagtg acaatggcga ctggagcaac ggcaaatgca actgtaactg atgcaaatac
     2341 tactaaagct acaactatca cttcaggcgg tacacctgtt cagattgata atactgcagg
     2401 ttccgcaact gccaaccttg gtgctgttag cttagtaaaa ctgcaggatt ccaagggtaa
     2461 tgataccgat acatatgcgc ttaaagatac aaatggcaat ctttacgctg cggatgtgaa
     2521 tgaaactact ggtgctgttt ctgttaaaac tattacctat actgactctt ccggtgccgc
     2581 cagttctcca accgcggtca aactgggcgg agatgatggc aaaacagaag tggtcgatat
     2641 tgatggtaaa acatacgatt ctgccgattt aaatggcggt aatctgcaaa caggtttgac
     2701 tgctggtggt gaggctctga ctgctgttgc aaatggtaaa accacggatc cgctgaaagc
     2761 gctggacgat gctatcgcat ctgtagacaa attccgttct tccctcggtg cggtgcaaaa
     2821 ccgtctggat tccgcggtta ccaacctgaa caacaccact accaacctgt ctgaagcgca
     2881 gtcccgtatt caggacgccg actatgcgac cgaagtgtcc aatatgtcga aagcgcagat
     2941 catccagcag gccggtaact ccgtgttggc aaaagctaac caggtaccgc agcaggttct
     3001 gtctctgctg cagggttaag aattctgatc acactggcta cctttgggtg gccttttttt
     3061 atcgtcaaaa gggcgacacc ccctaattag cccgggcgaa aggcccagtc tttcgactga
     3121 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcatggggag tccccacact
     3181 accatcggcg ctacggcgtt tcacttctga gttcggcatg gggtcaggtg ggaccaccgc
     3241 gctactgccg ccaggcaaac aaggggtgtt atgagccata ttcaggtata aatgggctcg
     3301 cgataatgtt cagaattggt taattggttg taacactgac ccctatttgt ttatttttct
     3361 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3421 attgaaaaag gaagaatatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     3481 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     3541 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     3601 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     3661 aatttatgcc acttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     3721 tcaccactgc gatccccgga aaaacagcgt tccaggtatt agaagaatat cctgattcag
     3781 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcactcg attcctgttt
     3841 gtaattgtcc ttttaacagc gatcgcgtat ttcgcctcgc tcaggcgcaa tcacgaatga
     3901 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     3961 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     4021 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     4081 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     4141 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     4201 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaagcg gcgcgccatc
     4261 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
     4321 gtggtgaata tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
     4381 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     4441 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
     4501 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
     4561 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
     4621 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
     4681 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
     4741 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
     4801 agtattattt tctcccatga ggacggtacg cgactgggcg tggagcatct ggtcgcattg
     4861 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
     4921 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
     4981 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
     5041 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
     5101 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
     5161 gatagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
     5221 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     5281 ctgttgccag tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     5341 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     5401 agcgggcagt gactcatgac caaaatccct taacgtgagt tacgcgcgcg tcgttccact
     5461 gagcgtcaga c
//
