LOCUS Exported 2710 bp ds-DNA circular SYN 20-JUN-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2710) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 20, 2019 from SnapGene 4.0.6 http://www.snapgene.com COMMENT LOCUS pUC57 2710 bp DNA SYN DEFINITION pUC57 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..2710 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature complement(250..398) /label=lacZ_a misc_feature 364..386 /label=M13_pUC_fwd_primer misc_feature complement(502..524) /label=M13_pUC_rev_primer misc_feature 873..1555 /label=ColE1 Origin /note="ColE1 Origin" gene complement(1650..2510) /label=Ampicillin misc_feature 1790..1796 /label=BsaI site destroyed /note="BsaI site destroyed" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa 421 tgcatctaga tatcggatcc cgggcccgtc gactgcagag gcctgcatgc aagcttggcg 481 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 541 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 661 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 1021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 1081 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 1141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 1201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 1261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 1321 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 1381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 1441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 1501 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 1561 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 1621 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 1681 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 1741 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg aaacccacgc 1801 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 1861 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 1921 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 1981 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 2041 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 2101 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 2161 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 2221 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 2281 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 2341 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 2401 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 2461 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 2521 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 2581 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 2641 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 2701 ccctttcgtc //