LOCUS       Exported                2953 bp ds-DNA     circular SYN 18-JUN-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pDV (pUC57-CmR-LacZa-SV40pA-BsaI-BpiI)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 20, 2019 from SnapGene 4.0.6
            http://www.snapgene.com
COMMENT     LOCUS pUC57 2710 bp DNA SYN DEFINITION pUC57 ACCESSION KEYWORDS 
            SOURCE ORGANISM other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    185..1104
                     /label=Untitled
                     /note="Untitled"
     misc_feature    238..258
                     /label=M13-Fwd (BR-8)
                     /note="M13-Fwd (BR-8)"
     misc_feature    306..658
                     /label=LacZa
                     /note="LacZa"
     misc_feature    690..715
                     /label=M13-Rev (BR-9)
                     /note="M13-Rev (BR-9)"
     V_segment       716..945
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    1317..1999
                     /label=ColE1 Origin
                     /note="ColE1 Origin"
     misc_feature    2097..2753
                     /label=CmR
                     /note="CmR"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgtt
      241 gtaaaacgac ggccagtgtc gacagatctc tagaggatcc gaattcgaag acaaatccag
      301 agacctgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
      361 aggaactaaa ccatgataga tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      421 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      481 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
      541 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
      601 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatta aggtctcacc
      661 atttgtcttc tcgaggcggc cgcacgcgtg taatcatggt catagctgtt tcctgtgatc
      721 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      781 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      841 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      901 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacctgt gtgaaattgt
      961 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     1021 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     1081 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1141 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1201 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1261 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1321 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1381 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1441 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1501 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1561 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1621 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1681 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1741 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1801 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1861 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1921 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1981 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2041 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttacgcc
     2101 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     2161 ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     2221 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     2281 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     2341 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     2401 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     2461 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     2521 ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     2581 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     2641 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     2701 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc catactcttc
     2761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2881 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2941 aggccctttc gtc
//
