LOCUS Exported 3846 bp ds-DNA circular SYN 21-JUN-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3846) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 21, 2019 from SnapGene 4.0.6 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3846 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 468..683 /gene="S. cerevisiae URA3" /label=URA3 promoter CDS 684..1487 /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /label=URA3 /note="yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" primer_bind complement(1625..1641) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" rep_origin 2009..2691 /label=ColE1 Origin /note="ColE1 Origin" CDS complement(2786..3646) /label=Ampicillin promoter complement(3647..3751) /gene="bla" /label=AmpR promoter ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa 421 tgcatctaga tatcggatcc cggtctcgag caaccacagc ttttcaattc aattcatcat 481 ttttttttta ttcttttttt tgatttcggt ttctttgaaa tttttttgat tcggtaatct 541 ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat 601 gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa 661 cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc 721 atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt 781 gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc 841 ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca 901 cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa 961 aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag 1021 cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt 1081 tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat 1141 tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt gacattgcga 1201 agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg 1261 aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagacgcat 1321 tgggtcaaca gtatagaacc gtggatgatg tggtgtctac aggatctgac attattattg 1381 ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa cgttacagaa 1441 aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat 1501 aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat 1561 taccctccat agagaccggg cccgtcgact gcagaggcct gcatgcaagc ttggcgtaat 1621 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 1681 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 1741 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 1801 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 1861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 1921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 1981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 2041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 2101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 2161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 2221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 2281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 2341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 2401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 2461 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 2521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 2581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 2641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 2701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 2761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 2821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 2881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgaaac ccacgctcac 2941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 3001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 3061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 3121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 3181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 3241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 3301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 3361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 3421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 3481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 3541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 3601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 3661 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 3721 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 3781 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 3841 ttcgtc //