LOCUS       Exported                3921 bp ds-DNA     circular SYN 21-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 21, 2019 from SnapGene 4.0.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        488..831
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             832..1641
                     /label=KanMX
                     /note="KanMX"
     primer_bind     complement(1700..1716)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2084..2766
                     /label=ColE1 Origin
                     /note="ColE1 Origin"
     CDS             complement(2861..3721)
                     /label=Ampicillin
     promoter        complement(3722..3826)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tatcggatcc cggtctcgag caagcttgcc tcgtccccgc cgggtcaccc
      481 ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg
      541 ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc
      601 atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac
      661 ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct
      721 gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt
      781 taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac catgggtaag
      841 gaaaagactc acgtttcgag gccgcgatta aattccaaca tggatgctga tttatatggg
      901 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg
      961 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1021 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     1081 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggcaaaaca
     1141 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1201 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     1261 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     1321 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt
     1381 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     1441 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     1501 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     1561 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     1621 atgctcgatg agtttttcta accatagaga ccgggcccgt cgactgcaga ggcctgcatg
     1681 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1741 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     1801 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1861 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1921 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1981 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2041 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2101 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2161 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2221 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2281 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2341 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2401 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2461 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2521 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2581 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2641 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2701 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2761 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2821 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2881 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2941 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3001 gaaacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3061 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3121 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3181 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3241 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3301 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3361 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3421 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3481 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3541 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3601 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3661 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3721 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3781 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3841 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     3901 gtatcacgag gccctttcgt c
//
