LOCUS       pLenti-5HRE-GFP_        8502 bp ds-DNA     circular SYN 25-JUN-2019
DEFINITION  Lentiviral backbone for use with GMAP.
ACCESSION   . (modified)
VERSION     .
KEYWORDS    LV 1-5.
SOURCE      null
  ORGANISM  
            .
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Akama-Garren, EH, Joshi, NS, Tammela, T, Chang, GP, Wagner, BL,
            Lee, DY, Rideout, Iii, WM, Papagiannakopoulos, T, Xue, W, Jacks and
            T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 10, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(46..65)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
     enhancer        237..616
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        618..816
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     primer_bind     771..791
                     /note="Human CMV immediate early promoter, forward primer"
                     /label="CMV-F"
     LTR             834..1014
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    1061..1186
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /label="HIV-1 Psi"
     misc_feature    1685..1918
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to
                     thecytoplasm."
                     /label="RRE"
     misc_feature    2445..2562
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     misc_feature    2625..2654
                     /created_by="User"
                     /label="GMAP site 1"
     source          order(2860..>4163,<2655..2859)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     2655..2673
                     /Sequence="TCGGTACCGAGCTTTTCTC"
                     /Hairpin_Tm="44.0"
                     /Tm="56.9"
                     /Self_Dimer_Tm="34.8"
                     /%GC="52.6"
                     /modified_by="User"
                     /label="pZM41.f (w/o GMAP site 1)"
     misc_feature    2677..2711
                     /created_by="User"
                     /label="VEGF HRE"
     primer_bind     2677..2694
                     /Sequence="CCACAGTGCATACGTGGG"
                     /Hairpin_Tm="42.5"
                     /Tm="58.1"
                     /Self_Dimer_Tm="19.2"
                     /%GC="61.1"
                     /created_by="primer3"
                     /label="pZM38.f (w/o GMAP site 1)"
     misc_feature    2718..2752
                     /created_by="User"
                     /label="VEGF HRE"
     misc_feature    2759..2793
                     /created_by="User"
                     /label="VEGF HRE"
     misc_feature    2800..2834
                     /created_by="User"
                     /label="VEGF HRE"
     misc_feature    2841..2875
                     /created_by="User"
                     /label="VEGF HRE"
     origin          2859^2860
     promoter        2886..2924
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="minimal CMV promoter"
     primer_bind     2921..2945
                     /note="Human CMV promoter, forward primer"
                     /label="LNCX"
     regulatory      2980..2989
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                      of translation (Kozak, 1987)"
     CDS             2986..3831
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461
                     of mouse ornithine decarboxylase, giving an in
                     vivohalf-life of ~2 hours"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACAS
                     ARINV"
                     /label="d2EGFP"
     primer_bind     complement(3031..3052)
                     /note="EGFP, reverse primer"
                     /label="EGFP-N"
     primer_bind     complement(3292..3311)
                     /note="For distinguishing EGFP variants, reverse primer"
                     /label="EXFP-R"
     primer_bind     3639..3660
                     /note="EGFP, forward primer"
                     /label="EGFP-C"
     misc_feature    complement(3809..3831)
                     /Sequence="CTACACATTGATCCTAGCAGAAG"
                     /Hairpin_Tm="None"
                     /Tm="56.5"
                     /Self_Dimer_Tm="None"
                     /%GC="43.5"
                     /created_by="primer3"
                     /label="pZM39.r (w/o GMAP site 5)"
                     /note="Geneious type: primer_bind_reverse"
     CDS             3841..3870
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
                     /label="Myc"
     primer_bind     complement(3906..3923)
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /label="BGH-rev"
     polyA_signal    3912..4136
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     misc_feature    complement(4146..4163)
                     /Sequence="CCCAGCTGGTTCTTTCCG"
                     /Hairpin_Tm="None"
                     /Tm="58.0"
                     /Self_Dimer_Tm="30.6"
                     /%GC="61.1"
                     /created_by="primer3"
                     /label="pZM43.r (w/o GMAP site 4)"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    4164..4193
                     /created_by="User"
                     /modified_by="User"
                     /label="GMAP site 4"
     source          <4194..>5338
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     4194..4214
                     /Sequence="AATTCTACCGGGTAGGGGAGG"
                     /Hairpin_Tm="47.0"
                     /Tm="60.4"
                     /Self_Dimer_Tm="18.7"
                     /%GC="57.1"
                     /modified_by="User"
                     /label="PGK 4 F (w/o GMAP site 4)"
     promoter        4203..4702
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     primer_bind     complement(4238..4261)
                     /note="Murine stem cell virus, reverse primer"
                     /label="MSCV-rev"
     primer_bind     4635..4654
                     /note="Mouse PGK promoter, forward primer"
                     /label="mPGK-F"
     CDS             4723..5322
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
                     /label="PuroR"
     primer_bind     complement(4723..4742)
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
                     /label="Puro-R"
     primer_bind     5219..5239
                     /note="Puromycin resistance gene, forward primer"
                     /label="Puro-F"
     misc_feature    complement(5319..5338)
                     /Sequence="ATTGTTCCAGACGCGGTCAG"
                     /Hairpin_Tm="34.6"
                     /Tm="60.7"
                     /Self_Dimer_Tm="11.3"
                     /%GC="55.0"
                     /created_by="primer3"
                     /label="Puro 5 R (w/o GMAP site 5)"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(5339..5368)
                     /created_by="User"
                     /label="GMAP site 5"
     misc_feature    5391..5979
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /label="WPRE"
     primer_bind     complement(5444..5464)
                     /note="WPRE, reverse primer"
                     /label="WPRE-R"
     CDS             complement(5862..5873)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
                     /label="Factor Xa site"
     primer_bind     complement(5982..5998)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="KS primer"
     primer_bind     complement(5983..5999)
                     /note="For pBluescript vector"
                     /label="pBluescriptKS"
     LTR             6508..6688
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     rep_origin      complement(6750..7338)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(6830..6849)
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     CDS             complement(7509..8369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     primer_bind     8132..8151
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     promoter        complement(8370..8474)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN      
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag
     1561 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1621 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1681 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1741 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1801 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1861 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1921 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1981 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2041 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2101 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2161 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2221 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2281 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2341 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     2401 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2461 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2521 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2581 agcagagatc cagtttggtt agtaccgggc ccgggtcgac tagtgatcag tgtgagggag
     2641 tgtaaagctg gttttcggta ccgagctttt ctcgagccac agtgcatacg tgggctccaa
     2701 caggtcctct tgtcgagcca cagtgcatac gtgggctcca acaggtcctc ttgtcgagcc
     2761 acagtgcata cgtgggctcc aacaggtcct cttgtcgagc cacagtgcat acgtgggctc
     2821 caacaggtcc tcttgtcgag ccacagtgca tacgtgggct ccaacaggtc ctcttgtcga
     2881 gatctggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     2941 agatcactag aagcttggca ttccggtact gttggtaaag ccaccatggt gagcaagggc
     3001 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3061 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3121 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3181 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3241 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3301 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3361 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3421 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     3481 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     3541 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     3601 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3661 accgccgccg ggatcactct cggcatggac gagctgtaca agaagctgag ccacggcttc
     3721 ccgccggagg tggaggagca ggatgatggc acgctgccca tgtcttgtgc ccaggagagc
     3781 gggatggacc gtcaccctgc agcctgtgct tctgctagga tcaatgtgta ggcggccgca
     3841 gaacaaaaac tcatctcaga agaggatctg aatggggccg catagtctag aagctcgctg
     3901 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3961 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4021 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4081 gggggaggat tgggaagaca atagcaggca tgctggggat ggcccgggct ctatggcttc
     4141 tgaggcggaa agaaccagct ggggacccga cattagcgct acagcttaag cggaattcta
     4201 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     4261 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
     4321 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     4381 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
     4441 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
     4501 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
     4561 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     4621 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     4681 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc
     4741 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     4801 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     4861 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     4921 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     4981 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     5041 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     5101 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     5161 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5221 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     5281 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct ggaacaataa
     5341 acgttgttgt ttggggttga attactcttc tagaccgcgg cgcgcccgat aatcaacctc
     5401 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5461 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5521 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5581 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5641 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5701 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5761 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     5821 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5881 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5941 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgat
     6001 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     6061 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     6121 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6181 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     6241 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     6301 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     6361 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     6421 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     6481 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     6541 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6601 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6661 ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa aaggccagca aaaggccagg
     6721 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6781 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6841 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6901 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6961 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7021 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7081 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7141 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7201 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7261 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7321 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7381 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7441 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7501 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7561 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7621 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7681 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7741 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7801 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7861 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7921 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7981 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8041 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8101 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8161 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8221 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8281 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8341 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8401 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8461 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
