LOCUS       pLenti-5HRE-GFP_        9282 bp ds-DNA     circular SYN 25-JUN-2019
DEFINITION  Lentiviral backbone for use with GMAP.
ACCESSION   . (modified)
VERSION     .
KEYWORDS    LV 1-5.
SOURCE      null
  ORGANISM  
            .
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Akama-Garren, EH, Joshi, NS, Tammela, T, Chang, GP, Wagner, BL,
            Lee, DY, Rideout, Iii, WM, Papagiannakopoulos, T, Xue, W, Jacks and
            T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 10, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9282
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(46..65)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
     enhancer        237..616
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        618..816
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     primer_bind     771..791
                     /note="Human CMV immediate early promoter, forward primer"
                     /label="CMV-F"
     LTR             834..1014
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    1061..1186
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /label="HIV-1 Psi"
     misc_feature    1685..1918
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to
                     thecytoplasm."
                     /label="RRE"
     misc_feature    2445..2562
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     primer_bind     2564..2583
                     /Sequence="GGGTTTATTACAGGGACAGC"
                     /Hairpin_Tm="None"
                     /Tm="55.8"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="spZM8.f"
     misc_feature    2625..2654
                     /created_by="User"
                     /label="GMAP site 1"
     source          order(2860..>6118,<2655..2859)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     2655..2673
                     /Sequence="TCGGTACCGAGCTTTTCTC"
                     /Hairpin_Tm="44.0"
                     /Tm="56.9"
                     /Self_Dimer_Tm="34.8"
                     /%GC="52.6"
                     /modified_by="User"
                     /label="pZM41.f (w/o GMAP site 1)"
     misc_feature    2677..2711
                     /created_by="User"
                     /label="VEGF HRE"
     primer_bind     2677..2694
                     /Sequence="CCACAGTGCATACGTGGG"
                     /Hairpin_Tm="42.5"
                     /Tm="58.1"
                     /Self_Dimer_Tm="19.2"
                     /%GC="61.1"
                     /created_by="primer3"
                     /label="pZM38.f (w/o GMAP site 1)"
     misc_feature    2718..2752
                     /created_by="User"
                     /label="VEGF HRE"
     misc_feature    2759..2793
                     /created_by="User"
                     /label="VEGF HRE"
     misc_feature    2800..2834
                     /created_by="User"
                     /label="VEGF HRE"
     misc_feature    2841..2875
                     /created_by="User"
                     /label="VEGF HRE"
     origin          2859^2860
     promoter        2886..2924
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="minimal CMV promoter"
     primer_bind     2921..2945
                     /note="Human CMV promoter, forward primer"
                     /label="LNCX"
     regulatory      2980..2989
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                      of translation (Kozak, 1987)"
     CDS             2986..3831
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461
                     of mouse ornithine decarboxylase, giving an in
                     vivohalf-life of ~2 hours"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACAS
                     ARINV"
                     /label="d2EGFP"
     primer_bind     complement(3031..3052)
                     /note="EGFP, reverse primer"
                     /label="EGFP-N"
     primer_bind     complement(3292..3311)
                     /note="For distinguishing EGFP variants, reverse primer"
                     /label="EXFP-R"
     primer_bind     3457..3475
                     /Sequence="CAGAAGAACGGCATCAAGG"
                     /Hairpin_Tm="None"
                     /Tm="56.3"
                     /Self_Dimer_Tm="None"
                     /%GC="52.6"
                     /created_by="primer3"
                     /label="spZM10.f"
     primer_bind     3639..3660
                     /note="EGFP, forward primer"
                     /label="EGFP-C"
     misc_feature    complement(3809..3831)
                     /Sequence="CTACACATTGATCCTAGCAGAAG"
                     /Hairpin_Tm="None"
                     /Tm="56.5"
                     /Self_Dimer_Tm="None"
                     /%GC="43.5"
                     /created_by="primer3"
                     /label="pZM39.r (w/o GMAP site 5)"
                     /note="Geneious type: primer_bind_reverse"
     CDS             3841..3870
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
                     /label="Myc"
     primer_bind     complement(3906..3923)
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /label="BGH-rev"
     polyA_signal    3912..4136
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4182..4610
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     complement(4269..4288)
                     /note="F1 origin, reverse primer"
                     /label="F1ori-R"
     primer_bind     4448..4465
                     /Sequence="CCTTTGACGTTGGAGTCC"
                     /Hairpin_Tm="40.3"
                     /Tm="55.3"
                     /Self_Dimer_Tm="0.7"
                     /%GC="55.6"
                     /created_by="primer3"
                     /label="spZM11.f"
     primer_bind     4479..4500
                     /note="F1 origin, forward primer"
                     /label="F1ori-F"
     primer_bind     complement(4619..4639)
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /label="pBABE 3'"
     promoter        4624..4953
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     rep_origin      4804..4939
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     4866..4885
                     /note="SV40 promoter/origin, forward primer"
                     /label="SV40pro-F"
     CDS             4979..5773
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and G418
                      (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /label="NeoR/KanR"
     primer_bind     complement(5033..5052)
                     /note="Neomycin resistance gene, reverse primer"
                     /label="Neo-R"
     primer_bind     5643..5662
                     /note="Neomycin resistance gene, forward primer"
                     /label="Neo-F"
     polyA_signal    5947..6068
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5984..6003)
                     /note="SV40 polyA, reverse primer"
                     /label="SV40pA-R"
     primer_bind     6038..6057
                     /note="SV40 polyA terminator, reverse primer"
                     /label="EBV-rev"
     misc_feature    complement(6101..6118)
                     /Sequence="AGCGAGTCAGTGAGCGAG"
                     /Hairpin_Tm="34.3"
                     /Tm="59.1"
                     /Self_Dimer_Tm="16.9"
                     /%GC="61.1"
                     /modified_by="User"
                     /label="pZM42.r (w/o GMAP site 5)"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(6101..6118)
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     misc_feature    complement(6119..6148)
                     /created_by="User"
                     /label="GMAP site 5"
     misc_feature    6171..6759
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /label="WPRE"
     misc_feature    complement(6224..6244)
                     /Sequence="CATAGCGTAAAAGGAGCAACA"
                     /Hairpin_Tm="None"
                     /Tm="56.6"
                     /Self_Dimer_Tm="None"
                     /%GC="42.9"
                     /created_by="primer3"
                     /label="spZM9.r"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(6224..6244)
                     /note="WPRE, reverse primer"
                     /label="WPRE-R"
     CDS             complement(6642..6653)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
                     /label="Factor Xa site"
     primer_bind     complement(6762..6778)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="KS primer"
     primer_bind     complement(6763..6779)
                     /note="For pBluescript vector"
                     /label="pBluescriptKS"
     LTR             7288..7468
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     rep_origin      complement(7530..8118)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(7610..7629)
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     CDS             complement(8289..9149)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     primer_bind     8912..8931
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     promoter        complement(9150..9254)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN      
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag
     1561 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1621 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1681 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1741 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1801 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1861 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1921 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1981 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2041 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2101 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2161 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2221 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2281 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2341 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     2401 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2461 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2521 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2581 agcagagatc cagtttggtt agtaccgggc ccgggtcgac tagtgatcag tgtgagggag
     2641 tgtaaagctg gttttcggta ccgagctttt ctcgagccac agtgcatacg tgggctccaa
     2701 caggtcctct tgtcgagcca cagtgcatac gtgggctcca acaggtcctc ttgtcgagcc
     2761 acagtgcata cgtgggctcc aacaggtcct cttgtcgagc cacagtgcat acgtgggctc
     2821 caacaggtcc tcttgtcgag ccacagtgca tacgtgggct ccaacaggtc ctcttgtcga
     2881 gatctggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     2941 agatcactag aagcttggca ttccggtact gttggtaaag ccaccatggt gagcaagggc
     3001 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3061 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3121 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3181 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3241 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3301 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3361 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3421 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     3481 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     3541 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     3601 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3661 accgccgccg ggatcactct cggcatggac gagctgtaca agaagctgag ccacggcttc
     3721 ccgccggagg tggaggagca ggatgatggc acgctgccca tgtcttgtgc ccaggagagc
     3781 gggatggacc gtcaccctgc agcctgtgct tctgctagga tcaatgtgta ggcggccgca
     3841 gaacaaaaac tcatctcaga agaggatctg aatggggccg catagtctag aagctcgctg
     3901 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3961 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4021 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4081 gggggaggat tgggaagaca atagcaggca tgctggggat ggcccgggct ctatggcttc
     4141 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4201 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4261 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4321 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctctc
     4381 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4441 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4501 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4561 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     4621 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4681 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4741 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4801 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgcccctagg ctgactaatt
     4861 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4921 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccccccggga ggtccacaat
     4981 ggttgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     5041 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     5101 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactcca
     5161 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     5221 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     5281 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     5341 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     5401 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     5461 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgta tgcccgacgg
     5521 cgaggatctc gtcgtgactc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     5581 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     5641 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     5701 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     5761 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     5821 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5881 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     5941 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6001 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6061 gtatcttatc atgtctgtat accgtcgatc tttccgcttc ctcgctcact gactcgctaa
     6121 acgttgttgt ttggggttga attactcttc tagaccgcgg cgcgcccgat aatcaacctc
     6181 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6241 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6301 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6361 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6421 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6481 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6541 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     6601 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6661 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6721 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgat
     6781 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     6841 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     6901 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6961 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     7021 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     7081 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     7141 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     7201 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     7261 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     7321 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7381 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7441 ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa aaggccagca aaaggccagg
     7501 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7561 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7621 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7681 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7741 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7801 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7861 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7921 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7981 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8041 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8101 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8161 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8221 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8281 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8341 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8401 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8461 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8521 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8581 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8641 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8701 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8761 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8821 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8881 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8941 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9001 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9061 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9121 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9181 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9241 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
