LOCUS       pORTMAGE302A            7135 bp ds-DNA     circular     21-JUN-2019
DEFINITION  .
ACCESSION   urn.local...11-am8jwmf
KEYWORDS    .
SOURCE      
  ORGANISM  .
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Timothy M. Wannier, Akos Nyerges, Helene M Kuchwara, Marton
            Czikkely, David Balogh, Gabriel T Filsinger, Christopher J Gregg,
            Marc J Lajoie, Xavier Rios, Csaba Pal and George M Church
  TITLE     Improved bacterial recombineering by parallelized protein discovery
  JOURNAL   Unpublished, see
            http://group.szbk.u-szeged.hu/sysbiol/EvGEn/index.html and
            http://arep.med.harvard.edu/ KEYWORDS "pORTMAGE, Multiplex
            Automated Genome Engineering (MAGE)" SOURCE synthetic ORGANISM
            synthetic DNA construct
FEATURES             Location/Qualifiers
     misc_feature    complement(149..1996)
                     /note="Geneious type: gene"
                     /standard_name="mutL E32K"
     misc_feature    complement(2014..2760)
                     /note="Geneious type: recobinase"
                     /standard_name="PapRecT"
     promoter        complement(2830..2874)
     misc_feature    complement(3508..4302)
                     /note="Geneious type: gene"
                     /standard_name="KanR"
     misc_feature    complement(4570..5283)
                     /note="Geneious type: gene"
                     /standard_name="cI repressor"
     misc_feature    5417..7135
                     /note="Geneious type: rep_origin"
                     /standard_name="pBBRI origin"
     gene            6412..7074
                     /gene="rep"
                     /standard_name="rep"
ORIGIN      
        1 cagctgtctc ttatacacat ctccgctgtg ctttcagtgg atttcggata acagaaaggc
       61 cgggaaatac ccagcctcgc tttgtaacgg agtagacgaa agtgattgcg cctacccgga
      121 tattatcgtg aggatgctca tcgccatttc actcatcttt cagggctttt atcgccggat
      181 gtaaatcaac agattgtaac agaccacccg gcggcgtttt cacaagttgc ggacataacc
      241 gttccacgtc cgccagcagg gttatggcct gtgccattga ccactgcgca tgttcgctca
      301 tcagatttcg tgcaatccac tgcgcaatat tgccaggttc gaatacggac tgcttcgcca
      361 ggtagcctat cagttcagga atcaagattt gtaaattttg ttggcgtaag ggtaaaggca
      421 ctgccctgat ggtcacatgc tgtgcatctg actggaaatc aatacccaat tccgccaggg
      481 cagactgcgc tttttctaat gccgattttt cttcggcaga aacttttagc cgcaacggaa
      541 tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt caattgtgcc tgacgcagcc
      601 aacgttctgc cactggcaag gataaaagtg aaatgttgcc gtcgcgctcc agcaacgcac
      661 agtcggaatg gacgatagtc agtacccgac caaaactctg actgttcgcc gcaagtgcag
      721 gttcctgcgg ttccggcgct tttaattttt gcatcggcgc gggcgtttgc aaaagctggc
      781 gatacacttc accttgctgt ttctggtagc ctggctgcgc attcggccag ggggcagccg
      841 gacgactgcc tgatgctggc gcaggagtgt agcgcggagc taccggctca cgagctgccg
      901 gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc cggaatggaa cgcggtgcag
      961 gttggggttc atcgtccagc ggtagcggcg tttccagttg ctgttgtagc acgctcagca
     1021 cgccctgata gataaaatca tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg
     1081 cggggtgcac gttgacgtcc acctgatgtg ggtcgatctc cagatacaac acaaatgccg
     1141 gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg gatcgcgtga ttgatcaggc
     1201 gatcgcgcat catgcgaccg ttcacgtagc aatactgaat ttctgccagt gcgggcgtgg
     1261 tgtgatttgg atcggccacc cagccgcgta gcgtgagatc gccgtgttgc cattcaatcg
     1321 ccagcgcttg ttcaagaaaa gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc
     1381 cgccttccgg cactgcgcgg tactgacgca caattttacc gttatgcgac aggttgatcg
     1441 tgacgtcgaa acgcgccagc gcaatgcggc ggatgatctc atcaatgtgg ttaaattcgg
     1501 ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt gtagaacaga tccagcacct
     1561 ccagcgtcgt ccccacagga tgcgccgccg gttttaccgt cacgttcata tcgcgccctt
     1621 cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg tgaagtgagc gtcaggcggg
     1681 aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc caggctgata atggcttcga
     1741 gatcgtccag agaggcgatt ttactggtgg catgacgagc cagcgccagc gccagctcat
     1801 cttttttgat accgcagccg ttatcacgaa tgcggataag tttcgcccca ccgcgttcga
     1861 tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt tttcactagt tctttgacta
     1921 ccgacgcagg tcgctcgacc acctcacctg cggcaatctg gttcgccagt tgtggcggta
     1981 agacctgaat tggcattttg taatcctcct ctcttacgca gcacgatcat ctccagtttc
     2041 ttcgaattcg acatcaatgg gcgcatccaa cagctccttt tttctctggt ctttagcagc
     2101 agtcagttga tcacgggcac ctttagtctt gtaagctttc cacgcgtttg agaacgcggc
     2161 ctgcaagtcc tccatagttg gtgagtcttt gatcagacaa atcgcttcgg atacatcctc
     2221 gtattgctcg gcgggagtta catctcgttc aacaatgcgt tcggcctcat cctgatcgta
     2281 gatccccgcg aaaccaaagg ctagtctggc gcattgaatc atagccttat gacgcagcat
     2341 acgtcgcggg tgagactgcc aaggttgagt gttccttttg cactcagcca tgtactcggt
     2401 ggcgctgatg gcgtgcgagc ggtctttgcg gtaaatttta caagtgcatt cagttccctg
     2461 ttgatccata ctgaattcca tcccatcaaa ctggggattc tcgttaataa ttctagccca
     2521 accatcgact cccacaactg gaacaatacc attatttttg tcagggaacg cgtagagctc
     2581 ttttgtgaat ggattgagct tatattgatc cgcaacgatc aaaagggcta ccatctgact
     2641 atcgttcact tggcccttga aacaagtctg tttcagcgta ttagcgacct cttcgggggt
     2701 agttcccatc tcatagcggg tggcaaactt ggttaataac ggcgttagag cagtacccat
     2761 ggctgaactc ctgaaatagc tgtgaaaata tcgcccgcga aatgccgggc tgattagtaa
     2821 tccggaatcg cacttacggc caatgcttcg tttcgtatca cacaccccaa agccttctgc
     2881 tttgaatgct gcccttcttc agggcttaat ttttaagagc gtcaccttca tggtggtcag
     2941 tgcgtcctgc tgatgtgctc agtatcaccg ccagtggtat ttatgtcaac accgccagag
     3001 ataatttatc accgcagatg gttatctgta tgttttttat atgaatttat tttttgcagg
     3061 ggggcattgt ttggtaggtg agagatctga attgctatgt ttagtgagtt gtatctattt
     3121 atttttcaat aaatacaatt ggttatgtgt tttgggggcg atcgtgaggc aaagaaaacc
     3181 cggcgctgag gccgggttaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3241 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3301 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3361 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3421 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt aatgaagttt
     3481 tgacggtatc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
     3541 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
     3601 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
     3661 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
     3721 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tccgcgcctt
     3781 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
     3841 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     3901 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     3961 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     4021 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     4081 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tggagttcat tcagggcacc
     4141 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
     4201 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
     4261 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
     4321 tgtctcttga tccactagat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4381 acatattctc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4441 cggatacata tttgaatgaa ttcaaccatt tactatgtta tgttctgagg ggagtgaaaa
     4501 ttcccctaat tcgatgaaga ttcttgctca attgttatca gctatgcgcc gaccagaaca
     4561 ccttgccgat cagccaaacg tctcttcagg ccactgacta gcgataactt tccccacaac
     4621 ggaacaactc tcattgcatg ggatcattgg gtactgtggg tttagtggtt gtaaaaacac
     4681 ctgaccgcta tccctgatca gtttcttgaa ggtaaactca tcacccccaa gtctggctat
     4741 gcagaaatca cctggctcaa cagcctgctc agggtcaacg agaattaaca ttccgtcagg
     4801 aaagcttggc ttggagcctg ttggtgcggt catggaatta ccttcaacct caagccagaa
     4861 tgcagaatca ctggcttttt tggttgtgct tacccatctc tccgcatcac ctttggtaaa
     4921 ggttctaagc tcaggtgaga acatccctgc ctgaacatga gaaaaaacag ggtactcata
     4981 ctcacttcta agtgacggct gcatactaac cgcttcatac atctcgtaga tttctctggc
     5041 gattgaaggg ctaaattctt caacgctaac tttgagaatt tttgtaagca atgcggcgtt
     5101 ataagcattt aatgcattga tgccattaaa taaagcacca acgcctgact gccccatccc
     5161 catcttgtct gcgacagatt cctgggataa gccaagttca tttttctttt tttcataaat
     5221 tgctttaagg cgacgtgcgt cctcaagctg ctcttgtgtt aatggtttct tttttgtgct
     5281 catacgttaa atctatcacc gcaagggata aatatctaac accgtgcgtg ttgactattt
     5341 tacctctggc ggtgataatg gttgcatgta ctaaggaggt tgtatggaac aacgagatgt
     5401 gtataagaga cagctggcct gcccctccct tttggtgtcc aaccggctcg acgggggcag
     5461 cgcaaggcgg tgcctccggc gggccactca atgcttgagt atactcacta gactttgctt
     5521 cgcaaagtcg tgaccgccta cggcggctgc ggcgccctac gggcttgctc tccgggcttc
     5581 gccctgcgcg gtcgctgcgc tcccttgcca gcccgtggat atgtggacga tggccgcgag
     5641 cggccaccgg ctggctcgct tcgctcggcc cgtggacaac cctgctggac aagctgatgg
     5701 acaggctgcg cctgcccacg agcttgacca cagggattgc ccaccggcta cccagccttc
     5761 gaccacatac ccaccggctc caactgcgcg gcctgcggcc ttgccccatc aattttttta
     5821 attttctctg gggaaaagcc tccggcctgc ggcctgcgcg cttcgcttgc cggttggaca
     5881 ccaagtggaa ggcgggtcaa ggctcgcgca gcgaccgcgc agcggcttgg ccttgacgcg
     5941 cctggaacga cccaagccta tgcgagtggg ggcagtcgaa gggcgaagcc cgcccgcctg
     6001 ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg gggcagcgcc accttgggca
     6061 aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg gccacttttt gccggagggg
     6121 gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc cttctttggg caccaaagaa
     6181 ctagatatag ggcgaaatgc gaaagactta aaaatcaaca acttaaaaaa ggggggtacg
     6241 caacagctca ttgcggcacc ccccgcaata gctcattgcg taggttaaag aaaatctgta
     6301 attgactgcc acttttacgc aacgcataat tgttgtcgcg ctgccgaaaa gttgcagctg
     6361 attgcgcatg gtgccgcaac cgtgcggcac ccctaccgca tggagataag catggccacg
     6421 cagtccagag aaatcggcat tcaagccaag aacaagcccg gtcactgggt gcaaacggaa
     6481 cgcaaagcgc atgaggcgtg ggccgggctt attgcgagga aacccacggc ggcaatgctg
     6541 ctgcatcacc tcgtggcgca gatgggccac cagaacgccg tggtggtcag ccagaagaca
     6601 ctttccaagc tcatcggacg ttctttgcgg acggtccaat acgcagtcaa ggacttggtg
     6661 gccgagcgct ggatctccgt cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg
     6721 gtcaatgacc gcgtggcgtg gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt
     6781 gccgccgtgg tggttgatca cgacgaccag gacgaatcgc tgttggggca tggcgacctg
     6841 cgccgcatcc cgaccctgta tccgggcgag cagcaactac cgaccggccc cggcgaggag
     6901 ccgcccagcc agcccggcat tccgggcatg gaaccagacc tgccagcctt gaccgaaacg
     6961 gaggaatggg aacggcgcgg gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg
     7021 gacgatggcg agccgttgga gccgccgaca cgggtcacgc tgccgcgccg gtagcacttg
     7081 ggttgcgcag caacccgtaa gtgcgctgtt ccagactatc ggctgtaccg cctcg
//
