LOCUS       pMM281_pSV40NLS_        8788 bp ds-DNA     linear       10-SEP-2012
DEFINITION  This sequence has been entered manually.
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     gene            complement(3520..4614)
                     /gene="LEU2"
                     /label=LEU2
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            1813..2859
                     /gene="CIB1_ORF"
                     /label=CIB1_ORF
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1079..1476
                     /gene="pyADH1"
                     /label=pyADH1
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      3079..3405
                     /gene="tADH2"
                     /label=tADH2
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1492..2859
                     /label=SV40NLS_VP16_CIB1
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1561..1767
                     /label=VP16 (from GEV construct)
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1522..1542
                     /label=SV40NLS (PKKKRKV)
                     /ApEinfo_fwdcolor=#8080ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1813..2817
                     /label=CIB1 (protein sequence AT4G34530.1 from TAIR)
                     /ApEinfo_fwdcolor=#0080c0
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
     1141 gacaaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca
     1201 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc
     1261 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt
     1321 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta
     1381 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc
     1441 acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa gatggataaa
     1501 gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg taccgccgcc
     1561 tcggagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
     1621 ttcgatctgg acatgttggg ggacggggat tccccggggc cgggatttac cccccacgac
     1681 tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat gtttaccgat
     1741 gcccttggaa ttgacgagta cggtgggttt aatacgactc actatagggc gagcgccgaa
     1801 gctagcgcca ccatgaatgg agctatagga ggtgaccttt tgctcaattt tcctgacatg
     1861 tcggtcctag agcgccaaag ggctcacctc aagtacctca atcccacctt tgattctcct
     1921 ctcgccggct tctttgccga ttcttcaatg attaccggcg gcgagatgga cagctatctt
     1981 tcgactgccg gtttgaatct tccgatgatg tacggtgaga cgacggtgga aggtgattca
     2041 agactctcaa tttcgccgga aacgacgctt gggactggaa atttcaagaa acggaagttt
     2101 gatacagaga ctaaggattg taatgagaag aagaagaaga tgacgatgaa cagagatgac
     2161 ctagtagaag aaggagaaga agagaagtcg aaaataacag agcaaaacaa tgggagcaca
     2221 aaaagcatca agaagatgaa acacaaagcc aagaaagaag agaacaattt ctctaatgat
     2281 tcatctaaag tgacgaagga attggagaaa acggattata ttcatgttcg tgcacgacga
     2341 ggccaagcca ctgatagtca cagcatagca gaacgagtta gaagagaaaa gatcagtgag
     2401 agaatgaagt ttctacaaga tttggttcct ggatgcgaca agatcacagg caaagcaggg
     2461 atgcttgatg aaatcattaa ctatgttcag tctcttcaga gacaaatcga gttcttatcg
     2521 atgaaactag caattgtgaa tccaaggccg gattttgata tggatgacat ttttgccaaa
     2581 gaggttgcct caactccaat gactgtggtg ccatctcctg aaatggttct ttccggttat
     2641 tctcatgaga tggttcactc tggttattct agtgagatgg ttaactccgg ttaccttcat
     2701 gtcaatccaa tgcagcaagt gaataccagt tctgatccat tgtcatgctt caacaatggc
     2761 gaagctcctt cgatgtggga ctctcatgtg cagaatctct atggcaattt aggagtaccg
     2821 gtcatcgagc tcgagctgca gatgaatcgt agatactgaa aaaccccgca agttcacttc
     2881 aactgtgcat cgtgcaccat ctcaatttct ttcatttata catcgttttg ccttctttta
     2941 tgtaactata ctcctctaag tttcaatctt ggccatgtaa cctctgatct atagaatttt
     3001 ttaaatgact agaattaatg cccatctttt ttttggacct aaattcttca tgaaaatata
     3061 ttacgagggc ttattcagaa gctttggact tcttcgccag aggtttggtc aagtctccaa
     3121 tcaaggttgt cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa
     3181 tcgttggtag atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt
     3241 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     3301 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     3361 aggtatagca tgaggtcgct cttattgacc acacctctac cggccggtcg aaattcccct
     3421 accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat
     3481 aaagtttatg tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac
     3541 ttcttcggcg acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt
     3601 tctgatacct gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg
     3661 caagttcaat gacaatttca acatcattgc agcagacaag atagtggcga tagggttgac
     3721 cttattcttt ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt
     3781 gttcttgtct ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat
     3841 aacggaggct tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt
     3901 taggtgggtt gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac
     3961 cttcaatgta ggaaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga
     4021 agaggccaaa acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag
     4081 ggccatgaaa gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact
     4141 atcccaagcg acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac
     4201 taattctctg acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa
     4261 gtctaaaaga gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc
     4321 tttacggatt tttagtaaac cttgttcagg tctaacacta cctgtacccc atttaggacc
     4381 acccacagca cctaacaaaa cggcatcaac cttcttggag gcttccagcg cctcatctgg
     4441 aagtgggaca cctgtagcgt cgatagcagc accaccaatt aaatgatttt cgaaatcgaa
     4501 cttgacattg gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat
     4561 ttcttgacca acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat
     4621 ggtatatcct tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa
     4681 agtaagacga ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca
     4741 acttcaagta ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag
     4801 ccggttccgg cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat
     4861 gcgtcaggcg acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg
     4921 atagcgcccc tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc
     4981 gaactcttgc atcttacgat acctgagtat tcccacagtt ggggatctcg actctagcta
     5041 gaggatcaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5101 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5161 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5221 gtgccagctg ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag
     5281 tttacgtcca gccaagctag cttggctgca ggtcgagcgg ccgcgatccg gaacccttaa
     5341 tataacttcg tataatgtat gctatacgaa gttatcagct gcattaatga atcggccaac
     5401 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     5461 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5521 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5581 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5641 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5701 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5761 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5821 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5881 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5941 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6001 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     6061 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6121 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6181 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6241 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6301 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6361 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6421 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6481 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6541 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6601 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6661 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6721 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6781 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6841 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6901 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6961 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7021 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7081 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7141 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7201 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7261 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7321 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     7381 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     7441 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     7501 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     7561 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     7621 acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata tatacaggca
     7681 acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt
     7741 ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt cctattctct
     7801 agctagaaag tataggaact tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca
     7861 ctttcaaaaa accaaaaacg caccggactg taacgagcta ctaaaatatt gcgaataccg
     7921 cttccacaaa cattgctcaa aagtatctct ttgctatata tctctgtgct atatccctat
     7981 ataacctacc catccacctt tcgctccttg aacttgcatc taaactcgac ctctacattt
     8041 tttatgttta tctctagtat tactctttag acaaaaaaat tgtagtaaga actattcata
     8101 gagtgaatcg aaaacaatac gaaaatgtaa acatttccta tacgtagtat atagagacaa
     8161 aatagaagaa accgttcata attttctgac caatgaagaa tcatcaacgc tatcactttc
     8221 tgttcacaaa gtatgcgcaa tccacatcgg tatagaatat aatcggggat gcctttatct
     8281 tgaaaaaatg cacccgcagc ttcgctagta atcagtaaac gcgggaagtg gagtcaggct
     8341 ttttttatgg aagagaaaat agacaccaaa gtagccttct tctaacctta acggacctac
     8401 agtgcaaaaa gttatcaaga gactgcatta tagagcgcac aaaggagaaa aaaagtaatc
     8461 taagatgctt tgttagaaaa atagcgctct cgggatgcat ttttgtagaa caaaaaagaa
     8521 gtatagattc tttgttggta aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg
     8581 cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa
     8641 atgaagcaca gattcttcgt tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa
     8701 aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta
     8761 caaaatgaag cacagatgct tcgttgct   
//
