LOCUS       Exported                2363 bp ds-DNA     circular SYN 20-JUN-2019
DEFINITION  2358 bp    DNA     circular  2358 bp    DNA     circu lar  2400 bp  
             DNA     circu lar  2406 bp ds-DNA     circu lar SYN 08-JUL-2016 
            2676 bp d s-DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 20, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        14..710
                     /gene="pZiF_bs"
                     /label=pZiF_bs promoter
                     /note="PROMOTER PROMOTER BsaI_ZiF binding site_BsaI This is
                     the Zinc This is the Zinc Finger binding site part. It sho 
                     uld"
                     /note="This is the Zinc Finger binding site part. It 
                     should"
     misc_feature    complement(719..832)
                     /label=CamR Terminator
                     /note="CamR Terminator CamR Terminator CamR Terminator CamR
                     Terminator"
                     /note="CamR Terminator"
     CDS             complement(833..1492)
                     /codon_start=1
                     /gene="CDS"
                     /note="CDS CDS CDS CDS CamR CDS CDS CDS CDS"
                     /note="CDS"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1493..1597)
                     /gene="Promoter"
                     /note="PROMOTER PROMOTER PROMOTER PROMOTER CamR Promoter PR
                     OMOTER PR OMOTER PROMOTER PROMOTER"
                     /note="PROMOTER"
     rep_origin      complement(1598..2361)
                     /direction=LEFT
                     /gene="oMM1039"
                     /note="REP_ORIGIN REP_ORIGIN REP_ORIGIN REP_ORIGIN ColE1 RE
                     P_ORIGIN RE P_ORIGIN REP_ORIGIN REP_ORIGIN"
                     /note="REP_ORIGIN"
ORIGIN
        1 tcggtctcaa acgcgaggca agctaaacag atctttatat tgaattttca aaaattctta
       61 cttttttttt ggatggacgc aaagaagttt aataatcata ttacatggca ttaccaccat
      121 atacatatcc atatacatat ccatatctaa tcttacttat atgttgtgga aatgtaaaga
      181 gccccattat cttagcctaa aaaaaccttc tctttggaac tttcagtaat acgcttaact
      241 gctcattgct atattgaagt gcgcgcgcgc gtgggcgagc gtgggcgagc gtgggcgtct
      301 agaccgtgcg tcctcgtctt caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg
      361 cactgctccg aacaataaag attctacaat actagctttt atggttatga agaggaaaaa
      421 ttggcagtaa cctggcccca caaaccttca aattaacgaa tcaaattaac aaccatagga
      481 tgataatgcg attagttttt tagccttatt tctggggtaa ttaatcagcg aagcgatgat
      541 ttttgatcta ttaacagata tataaatgga aaagctgcat aaccacttta actaatactt
      601 tcaacatttt cagtttgtat tacttcttat tcaaatgtca taaaagtatc aacaaaaaat
      661 tgttaatata cctctatact ttaacgtcaa ggagaaaaaa ctataggtac tatgtgagac
      721 cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
      781 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
      841 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
      901 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
      961 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     1021 aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa
     1081 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     1141 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     1201 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1261 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1321 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1381 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1441 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     1501 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     1561 tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt
     1621 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1681 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1741 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1801 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1861 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1921 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1981 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2041 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2101 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2161 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2221 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2281 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2341 cctgattctg tggataaccg tag
//
