LOCUS       Exported                6754 bp ds-DNA     circular SYN 28-APR-2017
DEFINITION  6131 bp    DNA     circular  6131 bp    DNA     circ ular  4214 bp  
             DNA     circ ular  2293 bp    DNA     circ ular  17 79 bp    DNA   
             circ ular  52 83 bp ds-DNA     circ ular.
ACCESSION   .
VERSION     .
KEYWORDS    pMM553_pZiF_mRuby_LEU2_Leu2 homology
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 28, 2017 from SnapGene Viewer 3.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            5..504
                     /gene="LEU2 5' homology"
                     /note="LEU2 5' homology"
                     /note="GENE GENE;GENE"
     gene            505..524
                     /gene="LEU2 5' barcode"
                     /note="LEU2 5' barcode"
                     /note="GENE GENE;GENE"
     gene            544..686
                     /gene="ConS"
                     /note="ConS"
                     /note="GENE"
     misc_feature    694..718
                     /gene="ConS scar"
     promoter        723..1419
                     /gene="pZiF_bs"
                     /note="pZiF_bs promoter"
     CDS             1424..2133
                     /codon_start=1
                     /gene="mRuby2"
                     /note="mRuby2"
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     terminator      2138..2371
                     /gene="ScADH1"
                     /note="ScADH1 terminator"
     gene            complement(2376..2386)
                     /gene="BsmBI"
                     /note="BsmBI"
                     /note="GENE GENE;GENE"
     gene            2387..2529
                     /gene="Con1"
                     /note="Con1"
                     /note="GENE GENE;GENE"
     gene            2549..3196
                     /gene="LEU2 Promoter"
                     /note="LEU2 Promoter"
                     /note="GENE GENE GENE GENE;GENE"
     gene            3197..4291
                     /gene="LEU2"
                     /note="LEU2"
                     /note="GENE GENE GENE GENE;GENE"
     gene            4292..4465
                     /gene="AgLEU2 Terminator"
                     /note="AgLEU2 Terminator"
                     /note="GENE GENE GENE GENE;GENE"
     gene            4470..4489
                     /gene="LEU2 3' barcode"
                     /note="LEU2 3' barcode"
                     /note="GENE GENE GENE GENE GENE GENE;GENE"
     gene            4490..4979
                     /gene="LEU2 3' Homology"
                     /note="LEU2 3' Homology"
                     /note="GENE GENE GENE GENE GENE GENE;GENE"
     terminator      complement(4992..5018)
                     /gene="KanR Terminator"
                     /label=KanR Terminator
                     /note="KanR Terminator"
                     /note="TERMINATOR TERMINATOR TERMINATOR TERMINATOR TERMINAT
                     OR TERMINAT OR TERMINAT OR TERMINATOR TERMINATOR 
                     TERMINATOR;TERMINATOR"
     CDS             complement(5019..5834)
                     /codon_start=1
                     /gene="KanR"
                     /label=KanR
                     /note="KanR"
                     /note="CDS CDS CDS CDS CDS CDS CDS CDS CDS CDS;CDS"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(5835..5982)
                     /gene="KanR_Promoter"
                     /label=KanR Promoter
                     /note="KanR_Promoter"
                     /note="PROMOTER PROMOTER PROMOTER PROMOTER PROMOTER PROMOTE
                     R PROMOTE R PROMOTE R PROMOTER PROMOTER;PROMOTER"
     rep_origin      complement(5983..6746)
                     /direction=LEFT
                     /gene="ColE1"
                     /label=ColE1
                     /note="ColE1"
                     /note="REP_ORIGIN REP_ORIGIN REP_ORIGIN REP_ORIGIN REP_ORIG
                     IN REP_ORIG IN REP_ORIG IN REP_ORIGIN REP_ORIGIN 
                     REP_ORIGIN;REP_ORIGIN"
ORIGIN
        1 caatacccag aagaacagta aaataaagca aggtacgtga aattaatatt tttaaatggt
       61 tctaaccgat gccgaagaac tgcgcagtcc ggttataacg tctgacatgt ccttttttga
      121 tttggaatcc aaccactcaa gtgactctgt tcatttactt tgcgaaaaat atacccacaa
      181 attgcccatc gaaagtgaat cgcaaaccac cttcagactg gcaccgacaa agcaaagatt
      241 atacagacag agtactttat acgtaccgtt aagtctcaag caaagggttt tcttatttac
      301 tgaacgggta aagagtatct gggccggctt gccaagatgc aaaccgaata agtatttcaa
      361 agttgcattt gccttagccg tcctgacacc attggctatt tggatatttt atattgactt
      421 tcgtgtacat tgatcacatc gactgttcta ttggcaaatg aaccacgggc attgactatt
      481 tttcaggtta ctactatata ttatcatcac gggcaaggat tgtaccctga attcgcatct
      541 agatggtaga gccacaaaca gccggtacaa gcaacgatct ccaggaccat ctgaatcatg
      601 cgcggatgac acgaactcac gacggcgatc acagacatta acccacagta cagacactgc
      661 gacaacgtgg caattcgtcg caataccgtc tcactgaact ggccgataat tgcagacgaa
      721 cgcgaggcaa gctaaacaga tctttatatt gaattttcaa aaattcttac tttttttttg
      781 gatggacgca aagaagttta ataatcatat tacatggcat taccaccata tacatatcca
      841 tatacatatc catatctaat cttacttata tgttgtggaa atgtaaagag ccccattatc
      901 ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta
      961 tattgaagtg cgcgcgcgcg tgggcgagcg tgggcgagcg tgggcgtcta gaccgtgcgt
     1021 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
     1081 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
     1141 ctggccccac aaaccttcaa attaacgaat caaattaaca accataggat gataatgcga
     1201 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
     1261 taacagatat ataaatggaa aagctgcata accactttaa ctaatacttt caacattttc
     1321 agtttgtatt acttcttatt caaatgtcat aaaagtatca acaaaaaatt gttaatatac
     1381 ctctatactt taacgtcaag gagaaaaaac tataggtact atggtgtcca aaggagagga
     1441 gttaatcaag gaaaacatga gaatgaaagt tgtcatggag ggctccgtta atggtcacca
     1501 attcaagtgt acaggggaag gtgaaggtaa tccttacatg ggtacacaaa ctatgagaat
     1561 taaagtaatt gaaggcggac cactaccatt tgcatttgac attctggcaa cgtcattcat
     1621 gtacggatca cgaactttca tcaagtaccc taaaggtata ccagactttt tcaagcaatc
     1681 ttttccagag ggttttacat gggaaagggt tacaagatac gaagatgggg gtgtcgtcac
     1741 agttatgcaa gatacttcat tagaagatgg ctgccttgtc tatcatgtgc aagtaagagg
     1801 ggtgaatttt ccttctaacg gacctgtgat gcagaaaaag accaaaggtt gggaaccaaa
     1861 tactgaaatg atgtacccag ctgatggagg tttgagaggc tacacacaca tggcgcttaa
     1921 agttgatggt ggaggtcatt tgtcttgtag ttttgttacc acttatcgtt ctaaaaagac
     1981 tgttggcaat atcaaaatgc caggaataca tgctgtagac cacagactag aaagactcga
     2041 agagagcgat aacgaaatgt tcgttgtaca gagagagcat gccgtagcca aatttgctgg
     2101 cttaggcggt ggtatggatg aattgtataa gggatcctaa ctcgaggcga atttcttatg
     2161 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
     2221 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
     2281 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc
     2341 gagcaaatgc ctgcaaatcg ctccccattt cgctgccaat gagacgacgg ggtcatcacg
     2401 gctcatcatg cgccaaacaa atgtgtgcaa tacacgctcg gatgactgca tgatgaccgc
     2461 actgactggg gacagcagat ccacctaagc ctgtgagaga agcagacacc cgacagatca
     2521 aggcagttaa ctagtgcact gcagtacatc gaggagaact tctagtatat ctacatacct
     2581 aatattattg ccttattaaa aatggaatcc caacaattac atcaaaatcc acattctctt
     2641 caaaatcaat tgtcctgtac ttccttgttc atgtgtgttc aaaaacgtta tatttatagg
     2701 ataattatac tctatttctc aacaagtaat tggttgtttg gccgagcggt ctaaggcgcc
     2761 tgattcaaga aatatcttga ccgcagttaa ctgtgggaat actcaggtat cgtaagatgc
     2821 aagagttcga atctcttagc aaccattatt tttttcctca acataacgag aacacacagg
     2881 ggcgctatcg cacagaatca aattcgatga ctggaaattt tttgttaatt tcagaggtcg
     2941 cctgacgcat ataccttttt caactgaaaa attgggagaa aaaggaaagg tgagagcgcc
     3001 ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg catcacaata
     3061 cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg gttagcaatc
     3121 gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat atatatttca
     3181 aggatatacc attctaatgt ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt
     3241 tggtcaagaa atcacagccg aagccattaa ggttcttaaa gctatttctg atgttcgttc
     3301 caatgtcaag ttcgatttcg aaaatcattt aattggtggt gctgctatcg atgctacagg
     3361 tgttccactt ccagatgagg cgctggaagc ctccaagaag gctgatgccg ttttgttagg
     3421 tgctgtgggt ggtcctaaat ggggaaccgg tagtgttaga cctgaacaag gtttactaaa
     3481 aatccgtaaa gaacttcaat tgtacgccaa cttaagacca tgtaactttg catccgactc
     3541 tcttttagac ttatctccaa tcaagccaca atttgctaaa ggtactgact tcgttgttgt
     3601 cagagaatta gtgggaggta tttactttgg taagagaaag gaggacgatg gtgatggtgt
     3661 cgcttgggat agtgaacaat acaccgttcc agaagtgcaa agaatcacaa gaatggccgc
     3721 tttcatggcc ctacaacatg agccaccatt gcctatttgg tccttggata aagctaatgt
     3781 tttggcctct tcaagattat ggagaaaaac tgtggaggaa accatcaaga acgaatttcc
     3841 tacattgaag gttcaacatc aattgattga ttctgccgcc atgatcctag ttaagaaccc
     3901 aacccaccta aatggtatta taatcaccag caacatgttt ggtgatatca tctccgatga
     3961 agcctccgtt atcccaggtt ccttgggttt gttgccatct gcgtccttgg cctctttgcc
     4021 agacaagaac accgcatttg gtttgtacga accatgccac ggttctgctc cagatttgcc
     4081 aaagaataag gtcaacccta tcgccactat cttgtctgct gcaatgatgt tgaaattgtc
     4141 attgaacttg cctgaagaag gtaaggccat tgaagatgca gttaaaaagg ttttggatgc
     4201 aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg gtgatgctgt
     4261 cgccgaagaa gttaagaaaa tccttgctta acctggagga cccttctctt tagactattc
     4321 tactcttatg cacgtaaaaa attctaggaa atatgtatta actaggagta aaataaccgg
     4381 ctagtggcat tcatatagcc gtctgtttac atctacatca cacatttcga gtgtatatct
     4441 cgcaacgttg gcgttaaata ggcaggagta ctcgtatcgc atgtcggtgc gacacgaaat
     4501 tacaaaatgg aatatgttca tagggtagac gaaactatat acgcaatcta catacattta
     4561 tcaagaagga gaaaaaggag gatgtaaagg aatacaggta agcaaattga tactaatggc
     4621 tcaacgtgat aaggaaaaag aattgcactt taacattaat attgacaagg aggagggcac
     4681 cacacaaaaa gttaggtgta acagaaaatc atgaaactat gattcctaat ttatatattg
     4741 gaggattttc tctaaaaaaa aaaaaataca acaaataaaa aacactcaat gacctgacca
     4801 tttgatggag tttaagtcaa taccttcttg aaccatttcc cataatggtg aaagttccct
     4861 caagaatttt actctgtcag aaacggcctt aacgacgtag tcgacctcct cttcagtact
     4921 aaatctacca ataccaaatc tgatggaaga atgggctaat gcatcatcct tacccagcgc
     4981 cgagcggccg cgtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
     5041 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     5101 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     5161 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     5221 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     5281 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     5341 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag gcgaaatacg
     5401 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     5461 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     5521 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     5581 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     5641 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     5701 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     5761 ccatataaat cagcatccat gttggaattt aatcgcggcc tggagcaaga cgtttcccgt
     5821 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     5881 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     5941 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agtcatgacc aaaatccctt
     6001 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6061 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6121 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6181 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6241 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6301 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6361 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6421 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6481 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6541 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6601 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6661 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6721 tatcccctga ttctgtggat aaccgtgcgg ccgc
//
