LOCUS       bMM647_bCS43           11260 bp ds-DNA     circular     30-JUL-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      5397..5621
                     /label="tADH1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        1185..1884
                     /label="pRPL18B"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5674..7229
                     /label="LoxP-KIURA3-LoxP"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        3973..4669
                     /label="pZiF"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             2191..3701
                     /label="CRY2phr"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      3715..3939
                     /label="tSSA1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_recomb     complement(5690..5723)
                     /label="LoxP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    4674..5381
                     /label="mRuby2"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     rep_origin      8757..9439
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    join(11128..11260,1..913)
                     /label="CIB1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     nucleotide      9533..10032
                     /label="URA3 5' Homology"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     nucleotide      7254..7753
                     /label="URA3 3' Homology"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_recomb     complement(7176..7209)
                     /label="LoxP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     terminator      5397..5621
                     /label="tADH1"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     promoter        10101..10800
                     /label="pRPL18B"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             7793..8608
                     /label="KanR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     terminator      3715..3939
                     /label="tSSA1"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
     terminator      927..1151
                     /label="tENO1"
                     /ApEinfo_revcolor=#808000
                     /ApEinfo_fwdcolor=#808000
ORIGIN
        1 caatgattac cggcggcgag atggacagct atctttcgac tgccggtttg aatcttccga
       61 tgatgtacgg tgaaacgacg gtggaaggtg attcaagact ctcaatttcg ccggaaacga
      121 cgcttgggac tggaaatttc aagaaacgga agtttgatac agagactaag gattgtaatg
      181 agaagaagaa gaagatgacg atgaacagag atgacctagt agaagaagga gaagaagaga
      241 agtcgaaaat aacagagcaa aacaatggga gcacaaaaag catcaagaag atgaaacaca
      301 aagccaagaa agaagagaac aatttctcta atgattcatc taaagtgacg aaggaattgg
      361 agaaaacgga ttatattcat gttcgtgcac gacgaggcca agccactgat agtcacagca
      421 tagcagaacg agttagaaga gaaaagatca gtgagagaat gaagtttcta caagatttgg
      481 ttcctggatg cgacaagatc acaggcaaag cagggatgct tgatgaaatc attaactatg
      541 ttcagtctct tcagagacaa atcgagttct tatcgatgaa actagcaatt gtgaatccaa
      601 ggccggattt tgatatggat gacatttttg ccaaagaggt tgcctcaact ccaatgactg
      661 tggtgccatc tcctgaaatg gttctttccg gttattctca tgagatggtt cactctggtt
      721 attctagtga gatggttaac tccggttacc ttcatgtcaa tccaatgcag caagtgaata
      781 ccagttctga tccattgtca tgcttcaaca atggcgaagc tccttcgatg tgggactctc
      841 atgtgcagaa tctctatggc aatttaggag taccggtcat cgagctcgag ctgcagatga
      901 atcgtagata cggatcctaa ctcgagagct tttgattaag ccttctagtc caaaaaacac
      961 gtttttttgt catttatttc attttcttag aatagtttag tttattcatt ttatagtcac
     1021 gaatgtttta tgattctata tagggttgca aacaagcatt tttcatttta tgttaaaaca
     1081 atttcaggtt taccttttat tctgcttgtg gtgacgcgtg tatccgcccg ctcttttggt
     1141 cacccatgta tgctgccaaa ccagatgtca acacagctac aacgaagagg atgtccaata
     1201 ttttttttaa ggaataagga tacttcaaga ctagattccc ccctgcattc ccatcagaac
     1261 cgtaaacctt ggcgctttcc ttgggaagta ttcaagaagt gccttgtccg gtttctgtgg
     1321 ctcacaaacc agcgcgcccg atatggcttt cttttcactt atgaatgtac cagtacggga
     1381 caattagaac gctcctgtaa caatctcttt gcaaatgtgg ggttacattc taaccatgtc
     1441 acactgctga cgaaattcaa agtaaaaaaa aatgggacca cgtcttgaga acgatagatt
     1501 ttctttattt tacattgaac agtcgttgtc tcagcgcgct ttatgttttc attcatactt
     1561 catattataa aataacaaaa gaagaatttc atattcacgc ccaagaaatc aggctgcttt
     1621 ccaaatgcaa ttgacacttc attagccatc acacaaaact ctttcttgct ggagcttctt
     1681 ttaaaaaaga cctcagtaca ccaaacacgt tacccgacct cgttatttta cgacaactat
     1741 gataaaattc tgaagaaaaa ataaaaaaat tttcatactt cttgctttta tttaaaccat
     1801 tgaatgattt cttttgaaca aaactacctg tttcaccaaa ggaaatagaa agaaaaaatc
     1861 aattagaaga aaacaaaaaa caaaagatct atggccccca agaagaagag gaaggttggt
     1921 attcacgggg gtacccgccc atatgcttgc cctgtcgagt cctgcgatcg ccgcttttct
     1981 cgctcggatg agcttacccg ccatatccgc atccataccg gtcagaagcc cttccagtgt
     2041 cgaatctgca tgcgtaactt cagtcgtagt gaccacctta ccacccacat ccgcacccac
     2101 acaggcgaga agccttttgc ctgtgacatt tgtgggagga agtttgccag gagtgatgaa
     2161 cgcaagaggc ataccaaaat ccatacaggt gctagcttca tgaagatgga caaaaagact
     2221 atagtttggt ttagaagaga tctaaggatt gaggataatc ctgcattagc agcagctgct
     2281 cacgaaggat ctgtttttcc tgtcttcatt tggtgtcctg aagaagaagg acagttttat
     2341 cctggaagag cttcaagatg gtggatgaaa caatcacttg ctcacttatc tcaatccttg
     2401 aaggctcttg gatctgacct cactttaatc aaaacccaca acacgatttc agcgatcttg
     2461 gattgtatcc gcgttaccgg tgctacaaaa gtcgtcttta accacctcta tgatcctgtt
     2521 tcgttagttc gggaccatac cgtaaaggag aagctggtgg aacgtgggat ctctgtgcaa
     2581 agctacaatg gagatctatt gtatgaaccg tgggagatat actgcgaaaa gggcaaacct
     2641 tttacgagtt tcaattctta ctggaagaaa tgcttagata tgtcgattga atccgttatg
     2701 cttcctcctc cttggcggtt gatgccaata actgcagcgg ctgaagcgat ttgggcgtgt
     2761 tcgattgaag aactagggct ggagaatgag gccgagaaac cgagcaatgc gttgttaact
     2821 agagcttgga gtccaggatg gagcaatgct gataagttac taaatgagtt catcgagaag
     2881 cagttgatag attatgcaaa gaacagcaag aaagttgttg ggaattctac ttcactactt
     2941 tctccgtatc tccatttcgg ggaaataagc gtcagacacg ttttccagtg tgcccggatg
     3001 aaacaaatta tatgggcaag agataagaac agtgaaggag aagaaagtgc agatcttttt
     3061 cttaggggaa tcggtttaag agagtattct cggtatatat gtttcaactt cccgtttact
     3121 cacgagcaat cgttgttgag tcatcttcgg tttttccctt gggatgctga tgttgataag
     3181 ttcaaggcct ggagacaagg caggaccggt tatccgttgg tggatgccgg aatgagagag
     3241 ctttgggcta ccggatggat gcataacaga ataagagtga ttgtttcaag ctttgctgtg
     3301 aagtttcttc tccttccatg gaaatgggga atgaagtatt tctgggatac acttttggat
     3361 gctgatttgg aatgtgacat ccttggctgg cagtatatct ctgggagtat ccccgatggc
     3421 cacgagcttg atcgcttgga caatcccgcg ttacaaggcg ccaaatatga cccagaaggt
     3481 gagtacataa ggcaatggct tcccgagctt gcgagattgc caactgaatg gatccatcat
     3541 ccatgggacg ctcctttaac cgtactcaaa gcttctggtg tggaactcgg aacaaactat
     3601 gcgaaaccca ttgtagacat cgacacagct cgtgagctac tagctaaagc tatttcaaga
     3661 acccgtgaag cacagatcat gatcggagca gcagcccggg gatcctaact cgaggccaat
     3721 tggtgcggca attgataata acgaaaatgt cttttaatga tctgggtata atgaggaatt
     3781 ttccgaacgt ttttacttta tatatatata tacatgtaac atatattcta tacgctatag
     3841 agaaaggaaa tttttcaatt aaaaaaaaaa tagagaaaga gtttcacttc ttgattatcg
     3901 ctaacactaa tggttgaagt actgctactt taattttatg ctggatgcac acactggctt
     3961 aagatgacaa cgcgaggcaa gctaaacaga tctttatatt gaattttcaa aaattcttac
     4021 tttttttttg gatggacgca aagaagttta ataatcatat tacatggcat taccaccata
     4081 tacatatcca tatacatatc catatctaat cttacttata tgttgtggaa atgtaaagag
     4141 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg
     4201 ctcattgcta tattgaagtg cgcgcgcgcg tgggcgagcg tgggcgagcg tgggcgtcta
     4261 gaccgtgcgt cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc
     4321 actgctccga acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat
     4381 tggcagtaac ctggccccac aaaccttcaa attaacgaat caaattaaca accataggat
     4441 gataatgcga ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt
     4501 tttgatctat taacagatat ataaatggaa aagctgcata accactttaa ctaatacttt
     4561 caacattttc agtttgtatt acttcttatt caaatgtcat aaaagtatca acaaaaaatt
     4621 gttaatatac ctctatactt taacgtcaag gagaaaaaac tataggtact atggtgtcca
     4681 aaggagagga gttaatcaag gaaaacatga gaatgaaagt tgtcatggag ggctccgtta
     4741 atggtcacca attcaagtgt acaggggaag gtgaaggtaa tccttacatg ggtacacaaa
     4801 ctatgagaat taaagtaatt gaaggcggac cactaccatt tgcatttgac attctggcaa
     4861 cgtcattcat gtacggatca cgaactttca tcaagtaccc taaaggtata ccagactttt
     4921 tcaagcaatc ttttccagag ggttttacat gggaaagggt tacaagatac gaagatgggg
     4981 gtgtcgtcac agttatgcaa gatacttcat tagaagatgg ctgccttgtc tatcatgtgc
     5041 aagtaagagg ggtgaatttt ccttctaacg gacctgtgat gcagaaaaag accaaaggtt
     5101 gggaaccaaa tactgaaatg atgtacccag ctgatggagg tttgagaggc tacacacaca
     5161 tggcgcttaa agttgatggt ggaggtcatt tgtcttgtag ttttgttacc acttatcgtt
     5221 ctaaaaagac tgttggcaat atcaaaatgc caggaataca tgctgtagac cacagactag
     5281 aaagactcga agagagcgat aacgaaatgt tcgttgtaca gagagagcat gccgtagcca
     5341 aatttgctgg cttaggcggt ggtatggatg aattgtataa gggatcctaa ctcgaggcga
     5401 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
     5461 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc
     5521 tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta
     5581 ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt cgctgagcag ttacagagat
     5641 gttacgaacc actagtgcac tgcagTACAg caggtcgaca acccttaata taacttcgta
     5701 taatgtatgc tatacgaagt tattaggtct agagatccca atacaacaga tcacgtgatc
     5761 ttttgtaaga tgaagttgaa gtgagtgttg caccgtgcca atgcaggtgg ctattagatt
     5821 aaatatgtga tttgttctat taagtttcct gtataattaa tggggagcgc tgattctctt
     5881 ttggtacgct tcccatccag catttctgta tctttcacct tcaaccttag gatctctacc
     5941 cttggcgaaa agtcctctgc caacaatgat gatatctgat ccaccactta caacttcgtc
     6001 gacggttctg tactgctgac ccaatgcatc gcctttgtcg tctaaaccta cacctggggt
     6061 catgattagc caatcaaacc cttcttctct tcctcccata tcgttctgag caatgaaccc
     6121 aataacgaaa tctttatcac tctttgcaat atcaacggta cccttagtat attcaccgtg
     6181 tgctagagaa cccttggaag acaattcagc aagcatcaat aatccccttg gttctttggt
     6241 gacctcttgc gcaccttgtt tcaagccagc aacaatacca gcaccagtaa ccccgtgggc
     6301 gttggtgata tcagaccatt ctgcgatacg gtaaacgccc gatgtatatt gtaatttgac
     6361 tgtgttaccg atatcggcga attttctgtc ctcaaatatc aagaacttgt atttctctgc
     6421 caatgctttc aatggaacga cagtaccctc ataactgaaa tcatccaaga tatcaacgtg
     6481 tgttttcaaa aggcaaatgt atggacccaa cgtttcaaca agtttcaata gctcatcagt
     6541 cgaacgaacg tcaagagaag cacacaaatt ggtcttcttt tcatccatta aacgtaaaag
     6601 tttcgatgca accggacttg catgagtctc agctctactg gtatatgatt ttgtggacat
     6661 ggtgcaacta attgacggga gtgtattgac gctggcgtac tggctttcac aaaatggccc
     6721 aatcacaacc acatcttaga tagttgaaat gactttagat aacatcaatt gagatgagct
     6781 taatcatgtc aaagctaaaa gtgtcaccat gaacgacaat tcttaagcaa atcacgtgat
     6841 atagatccac gaataaccac catttgatgc tcgaggcaag taatgtgtgt aaaaaaatgc
     6901 gttaccacca tccaatgcag accgatcttc tacccagaat cacatatatt tatgtaccga
     6961 gtaccttttt tctatcttcc aattgcttct cccatatgat tgtctccgta agctcgaaat
     7021 ttctaagttg gattttaatc ttcacgcagg atgacagttc gatgagcttc tgaggagtgt
     7081 ttagaacata atcagtttat ccatggtcta tctcttcttg tcgctttttc tcctcgatag
     7141 aacctaaata aaacgagctc tcgagaaccc ttaatataac ttcgtataat gtatgctata
     7201 cgaagttatt aggtgatatc agatccactg agtagagcac ttgaatccac tgccccggga
     7261 atctcggtcg taatgatttc tataatgacg aaaaaaaaaa aattggaaag aaaaagcttc
     7321 atggccttta taaaaaggaa ctatccaata cctcgccaga accaagtaac agtattttac
     7381 ggggcacaaa tcaagaacaa taagacagga ctgtaaagat ggacgcattg aactccaaag
     7441 aacaacaaga gttccaaaaa gtagtggaac aaaagcaaat gaaggatttc atgcgtttgt
     7501 actctaatct ggtagaaaga tgtttcacag actgtgtcaa tgacttcaca acatcaaagc
     7561 taaccaataa ggaacaaaca tgcatcatga agtgctcaga aaagttcttg aagcatagcg
     7621 aacgtgtagg gcagcgtttc caagaacaaa acgctgcctt gggacaaggc ttgggccgat
     7681 aaggtgtact ggcgtatata tatctaatta tgtatctctg gtgtagccca tttttagcat
     7741 gtaaatataa agaccgagcg gccgcgtgtt acaaccaatt aaccaattct gattagaaaa
     7801 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     7861 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     7921 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     7981 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     8041 gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac
     8101 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     8161 cgaggcgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     8221 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     8281 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag
     8341 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     8401 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     8461 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     8521 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctggagc
     8581 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     8641 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
     8701 gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagtcat
     8761 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8821 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8881 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8941 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9001 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9061 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9121 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9181 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9241 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9301 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9361 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9421 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9481 gttctttcct gcgttatccc ctgattctgt ggataaccgt gcggccgcca atgctaaatt
     9541 cgagtgaaac acaggaagat cagaaaatcc tcatttcatc catattaaca ataatttcaa
     9601 atgtttattt gcattatttg aaactaggca agacaagcaa cgaaacgttt ttgaaaattt
     9661 tgagtatttt caataaattt gtagaggact cagatattga aaaaaagcta cagcaattaa
     9721 tacttgataa gaagagtatt gagaagggca acggttcatc atctcatgga tctgcacatg
     9781 aacaaacacc agagtcaaac gacgttgaaa ttgaggctac tgcgccaatt gatgacaata
     9841 cagacgatga taacaaaccg aagttatctg atgtagaaaa ggattaaaga tgctaagaga
     9901 tagtgatgat atttcataaa taatgtaatt ctatatatgt taattacctt ttttgcgagg
     9961 catatttatg gtgaaggata agttttgacc atcaaagaag gttaatgtgg ctgtggtttc
    10021 agggtccata aagcccacat ggataacatt acccctgaat tcgcatctag actgaactgg
    10081 ccgataattg cagacgaacg aagaggatgt ccaatatttt ttttaaggaa taaggatact
    10141 tcaagactag attcccccct gcattcccat cagaaccgta aaccttggcg ctttccttgg
    10201 gaagtattca agaagtgcct tgtccggttt ctgtggctca caaaccagcg cgcccgatat
    10261 ggctttcttt tcacttatga atgtaccagt acgggacaat tagaacgctc ctgtaacaat
    10321 ctctttgcaa atgtggggtt acattctaac catgtcacac tgctgacgaa attcaaagta
    10381 aaaaaaaatg ggaccacgtc ttgagaacga tagattttct ttattttaca ttgaacagtc
    10441 gttgtctcag cgcgctttat gttttcattc atacttcata ttataaaata acaaaagaag
    10501 aatttcatat tcacgcccaa gaaatcaggc tgctttccaa atgcaattga cacttcatta
    10561 gccatcacac aaaactcttt cttgctggag cttcttttaa aaaagacctc agtacaccaa
    10621 acacgttacc cgacctcgtt attttacgac aactatgata aaattctgaa gaaaaaataa
    10681 aaaaattttc atacttcttg cttttattta aaccattgaa tgatttcttt tgaacaaaac
    10741 tacctgtttc accaaaggaa atagaaagaa aaaatcaatt agaagaaaac aaaaaacaaa
    10801 agatctatgg ataaagcgga attaattccc gagcctccaa aaaagaagag aaaggtcgaa
    10861 ttgggtaccg ccgcctcgga gctccactta gacggcgagg acgtggcgat ggcgcatgcc
    10921 gacgcgctag acgatttcga tctggacatg ttgggggacg gggattcccc gggtccggga
    10981 tttacccccc acgactccgc cccctacggc gctctggata tggccgactt cgagtttgag
    11041 cagatgttta ccgatgccct tggaattgac gagtacggtg ggtttaatac gactcactat
    11101 agggcgagcg ccgaagctag cgccaccatg aatggagcta taggaggtga ccttttgctc
    11161 aattttcctg acatgtcggt cctagagcgc caaagggctc acctcaagta cctcaatccc
    11221 acctttgatt ctcctctcgc cggcttcttt gccgattctt
//