LOCUS       Exported                7342 bp ds-DNA     circular SYN 01-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 1, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    867..2798
                     /label=Rep
     misc_feature    3160..4512
                     /label=VP2
     misc_feature    3355..4512
                     /label=VP3
     CDS             4513..4566
                     /codon_start=1
                     /label=Lock Insert
                     /translation="PLGLARGDDDDGPLGLAR"
     misc_feature    4568..5022
                     /label=VP3(1)
     misc_feature    4568..5022
                     /label=VP2(1)
     misc_feature    complement(5195..5197)
                     /label=LazZ Start - rc
                     /label=LazZ\Start\-\rc
     misc_feature    complement(5198..5319)
                     /label=lac Promoter
                     /label=lac\Promoter
     rep_origin      5539..6206
                     /label=pUC ori
                     /label=pUC\ori
     misc_feature    complement(6357..7214)
                     /label=bla
     misc_feature    complement(7233..7298)
                     /label=bla Promoter (Rosenberg 1979)
                     /label=bla\Promoter\(Rosenberg\1979)
     misc_feature    complement(7233..7294)
                     /label=bla Promoter (Bennett 1981)
                     /label=bla\Promoter\(Bennett\1981)
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc agcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
      721 atccactagt tctagagtcc tgtattagag gtcacgtgag tgttttgcga cattttgcga
      781 caccatgtgg tcacgctggg tatttaagcc cgagtgagca cgcagggtct ccattttgaa
      841 gcgggaggtt tgaacgcgca gccgccatgc cggggtttta cgagattgtg attaaggtcc
      901 ccagcgacct tgacgagcat ctgcccggca tttctgacag ctttgtgaac tgggtggccg
      961 agaaggaatg ggagttgccg ccagattctg acatggatct gaatctgatt gagcaggcac
     1021 ccctgaccgt ggccgagaag ctgcagcgcg actttctgac ggaatggcgt cgtgtgagta
     1081 aggccccgga ggcccttttc tttgtgcaat ttgagaaggg agagagctac ttccacatgc
     1141 acgtgctcgt ggaaaccacc ggggtgaaat ctatggtttt gggacgtttc ctgagtcaga
     1201 ttcgcgaaaa actgattcag agaatttacc gcgggatcga gccgactttg ccaaactggt
     1261 tcgcggtcac aaagaccaga aatggtgccg gaggcgggaa caaggtggtg gatgagtgct
     1321 acatccccaa ttacttgctc cccaaaaccc agcctgagct ccagtgggcg tggactaata
     1381 tggaacagta tttaagcgcc tgtttgaatc tcacggagcg taaacggttg gtggcgcagc
     1441 atctgacgca cgtgtcgcag acgcaggagc agaacaaaga gaatcagaat cccaattctg
     1501 atgcgccggt gatcagatca aaaacttcag ccaggtacat ggagctggtc gggtggctcg
     1561 tggacaaggg gattacctcg gagaagcagt ggatacagga ggaccaggcc tcatacatct
     1621 ccttcaatgc ggcctccaac tcgcggtccc aaatcaaggc tgccttggac aatgcgggaa
     1681 agattatgag cctgactaaa accgcccccg actacctggt gggccagcag cccgtggagg
     1741 acatttccag caatcggatt tataaaattt tggaactaaa cgggtacgat ccccaatatg
     1801 cggcttccgt ctttctggga tgggccacga aaaagttcgg caagaggaac accatctggc
     1861 tgtttgggcc tgcaactacc gggaagacca acatcgcgga ggccatagcc cacactgtgc
     1921 ccttctacgg gtgcgtaaac tggaccaatg agaactttcc cttcaacgac tgtgtcgaca
     1981 agatggtgat ctggtgggag gaggggaaga tgaccgccaa ggtcgtggag tcggccaaag
     2041 ccattctcgg aggaagcaag gtgcgcgtgg accagaaatg caagtcctcg gcccagatag
     2101 acccgactcc cgtgatcgtc acctccaaca ccaacatgtg cgccgtgatt gacgggaact
     2161 caacgacctt cgaacaccag cagccgttgc aagaccggat gttcaaattt gaactcaccc
     2221 gccgtctgga tcatgacttt gggaaggtca ccaagcagga agtcaaagac tttttccggt
     2281 gggcaaagga tcacgtggtt gaggtggagc atgaattcta cgtcaaaaag ggtggagcca
     2341 agaaaagacc cgcccccagt gacgcagata taagtgagcc caaacgggtg cgcgagtcag
     2401 ttgcgcagcc atcgacgtca gacgcggaag cttcgatcaa ctacgcagac aggtaccaaa
     2461 acaaatgttc tcgtcacgtg ggcatgaatc tgatgctgtt tccctgcaga caatgcgaga
     2521 gaatgaatca gaattcaaat atctgcttca ctcacggaca gaaagactgt ttagagtgct
     2581 ttcccgtgtc agaatctcaa cccgtttctg tcgtcaaaaa ggcgtatcag aaactgtgct
     2641 acattcatca tatcatggga aaggtgccag acgcttgcac tgcctgcgat ctggtcaatg
     2701 tggatttgga tgactgcatc tttgaacaat aaatgattta aatcaggtat ggctgccgat
     2761 ggttatcttc cagattggct cgaggacact ctctctgaag gaataagaca gtggtggaag
     2821 ctcaaacctg gcccaccacc accaaagccc gcagagcggc ataaggacga cagcaggggt
     2881 cttgtgcttc ctgggtacaa gtacctcgga cccttcaacg gactcgacaa gggagagccg
     2941 gtcaacgagg cagacgccgc ggccctcgag cacgacaaag cctacgaccg gcagctcgac
     3001 agcggagaca acccgtacct caagtacaac cacgccgacg cggagtttca ggagcgcctt
     3061 aaagaagata cgtcttttgg gggcaacctc ggacgagcag tcttccaggc gaaaaagagg
     3121 gttcttgaac ctctgggcct ggttgaggaa cctgttaaga cggctccggg aaaaaagagg
     3181 ccggtagagc actctcctgt ggagccagac tcctcctcgg gaaccggaaa ggcgggccag
     3241 cagcctgcaa gaaaaagatt gaattttggt cagactggag acgcagactc agtacctgac
     3301 ccccagcctc tcggacagcc accagcagcc ccctctggtc tgggaactaa taccatggct
     3361 acaggcagtg gcgcaccaat ggcagacaat aacgagggtg ccgacggagt gggtaattcc
     3421 tcgggaaatt ggcattgcga ttccacatgg atgggcgaca gagtcatcac caccagcacc
     3481 cgaacctggg ccctgcccac ctacaacaac cacctctaca aacaaatttc cagccaatca
     3541 ggagcctcga acgacaatca ctactttggc tacagcaccc cttgggggta ttttgacttc
     3601 aacagattcc actgccactt ttcaccacgt gactggcaaa gactcatcaa caacaactgg
     3661 ggattccgac ccaagagact caacttcaag ctctttaaca ttcaagtcaa agaggtcacg
     3721 cagaatgacg gtacgacgac gattgccaat aaccttacca gcacggttca ggtgtttact
     3781 gactcggagt accagctccc gtacgtcctc ggctcggcgc atcaaggatg cctcccgccg
     3841 ttcccagcag acgtcttcat ggtgccacag tatggatacc tcaccctgaa caacgggagt
     3901 caggcagtag gacgctcttc attttactgc ctggagtact ttccttctca gatgctgcgt
     3961 accggaaaca actttacctt cagctacact tttgaggacg ttcctttcca cagcagctac
     4021 gctcacagcc agagtctgga ccgtctcatg aatcctctca tcgaccagta cctgtattac
     4081 ttgagcagaa caaacactcc aagtggaacc accacgcagt caaggcttca gttttctcag
     4141 gccggagcga gtgacattcg ggaccagtct aggaactggc ttcctggacc ctgttaccgc
     4201 cagcagcgag tatcaaagac atctgcggat aacaacaaca gtgaatactc gtggactgga
     4261 gctaccaagt accacctcaa tggcagagac tctctggtga atccgggccc ggctatggca
     4321 agccacaagg acgatgaaga aaagtttttt cctcagagcg gggttctcat ctttgggaag
     4381 caaggctcag agaaaacaaa tgtggacatt gaaaaggtca tgattacaga cgaagaggaa
     4441 atcaggacaa ccaatcccgt ggctacggag cagtatggtt ctgtatctac caacctccag
     4501 agaggagccg gcccactggg actggcacgt ggagacgacg atgatggacc actgggactg
     4561 gcacgtggcg ccaacagaca agcagctacc gcagatgtca acacacaagg cgttcttcca
     4621 ggcatggtct ggcaggacag agatgtgtac cttcaggggc ccatctgggc aaagattcca
     4681 cacacggacg gacattttca cccctctccc ctcatgggtg gattcggact taaacaccct
     4741 cctccacaga ttctcatcaa gaacaccccg gtgcctgcga atccttcgac caccttcagt
     4801 gcggcaaagt ttgcttcctt catcacacag tactccacgg gacaggtcag cgtggagatc
     4861 gagtgggagc tgcagaagga aaacagcaaa cgctggaatc ccgaaattca gtacacttcc
     4921 aactacaaca agtctgttaa tgtggacttt actgtggaca ctaatggcgt gtattcagag
     4981 cctcgcccca ttggcaccag atacctgact cgtaatctgt aattggcagg tcttgttaat
     5041 caataaaccg tttaattcgt ttcagttgaa ctttggtctc tgcgtatttc tttcttatct
     5101 agtttccatg ctctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
     5161 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5221 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5281 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5341 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5401 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5461 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5581 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5761 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6001 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     6061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6181 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6241 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6301 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6361 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6421 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6481 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6541 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     6601 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     6661 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     6721 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     6781 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     6841 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6901 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6961 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7021 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7081 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7141 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7201 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7261 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7321 acatttcccc gaaaagtgcc ac
//
