LOCUS       3-pSpdCas9-huTET1CD-T2A-mCherry(PX458).xdna    11404 bp      DNA     circular   19.06.2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : V8-PX 458 dCas9-2A-mCherryMut.xdna [9222  nt] 
            : (FseI[5438] / FseI[5438]) to PCR(pJFA344C7-Tet1-CD-Plasmid
            -Addgene49236.xdna) [2174  nt] : (FseI[5] / FseI[2183])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    1338..1340
                     /label=pUC ori
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="D10A" - label=pUC ori - ApEinfo_fwdcolor=#e8edc7 - ApEinfo_revcolor=#76fffa - 1092..1094 \ 
     misc_feature    1284..1304
                     /label=NLS
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=NLS - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - 1038..1058 \ 
     misc_feature    1338..1340
                     /label=(GAC)D10A(GCC)
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=(GAC)D10A(GCC) - ApEinfo_fwdcolor=#ff0c00 - ApEinfo_revcolor=#ff0c00 - 1092..1094 \ 
     misc_feature    3828..3830
                     /label=(CAT)H840A(GCC)
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=(CAT)H840A(GCC) - ApEinfo_fwdcolor=#ff002a - ApEinfo_revcolor=#ff002a - 3582..3584 \ 
     misc_feature    1254..1280
                     /label=HA-Tag
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=HA-Tag - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - 1008..1034 \ 
     misc_feature    7710..8417
                     /label=mCherry-Mut-BbsI
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    8138..8138
                     /label=Mut-G433A-Lysin
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    235..275
                     /label=cloning site
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=cloning site - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 235..275 \ 
     misc_feature    8427..8657
                     /label=bGH polyA
                     /SerialCloner_Color=&h408080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=bGH polyA - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 6312..6542 \ 
     rep_origin      10679..11361
                     /label=ColE1 origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=ColE1 origin - ApEinfo_fwdcolor=gray50 - ApEinfo_revcolor=gray50 - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 8564..9246 \ 
     rep_origin      8899..9205
                     /label=F1 ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=F1 ori - ApEinfo_fwdcolor=gray50 - ApEinfo_revcolor=gray50 - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 6784..7090 \ 
     rep_origin      8882..9337
                     /label=M13 origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 origin - ApEinfo_fwdcolor=gray50 - ApEinfo_revcolor=gray50 - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 6767..7222 \ 
     misc_feature    9724..10580
                     /label=AmpR
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=AmpR - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 7609..8465 \ 
     misc_feature    1..248
                     /label=humanU6 promoter
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=humanU6 promoter - ApEinfo_fwdcolor=#0080ff - ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 1..248 \ 
     misc_feature    441..1237
                     /label=CBh promoter
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CBh promoter - ApEinfo_fwdcolor=#f5010a - ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 441..1237 \ 
                     5444..7617
                     /label=insert from PCR(pJFA344C7-Tet1-CD-Plasmid-Addgene49236.xdna)
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(pJFA344C7-Tet1-CD-Plasmid-Addgene49236.xdna) [2174  nt] : (FseI[5] / FseI[2183]) \ 
     misc_feature    5454..7613
                     /label=Tet1-CD
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6219..6221
                     /label=H not mutated
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6225..6227
                     /label=D not mutated
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA 
      241 CGAAACACCg gGTCTTCgaG AAGACctgtt ttagagctaG AAAtagcaag ttaaaataag 
      301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcTTTTTTg ttttagagct 
      361 agaaatagca agttaaaata aggctagtcc gtTTTTagcg cgtgcgccaa ttctgcagac 
      421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 
      481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc 
      541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc 
      601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tGtgcccagt 
      661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta 
      721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac 
      781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg 
      841 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga 
      901 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc 
      961 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc 
     1021 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg 
     1081 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag 
     1141 ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac 
     1201 ctggagcacc tgcctgaaat cacttttttt caggttGGac cggTgccacc ATGTACCCAT 
     1261 ACGATGTTCC AGATTACGCT TCGCCGAAGA AAAAGCGCAA GGTCGAAGCG TCCGACAAGA 
     1321 AGTACAGCAT CGGCCTGGCC ATCGGCACCA ACTCTGTGGG CTGGGCCGTG ATCACCGACG 
     1381 AGTACAAGGT GCCCAGCAAG AAATTCAAGG TGCTGGGCAA CACCGACCGG CACAGCATCA 
     1441 AGAAGAACCT GATCGGAGCC CTGCTGTTCG ACAGCGGCGA AACAGCCGAG GCCACCCGGC 
     1501 TGAAGAGAAC CGCCAGAAGA AGATACACCA GACGGAAGAA CCGGATCTGC TATCTGCAAG 
     1561 AGATCTTCAG CAACGAGATG GCCAAGGTGG ACGACAGCTT CTTCCACAGA CTGGAAGAGT 
     1621 CCTTCCTGGT GGAAGAGGAT AAGAAGCACG AGCGGCACCC CATCTTCGGC AACATCGTGG 
     1681 ACGAGGTGGC CTACCACGAG AAGTACCCCA CCATCTACCA CCTGAGAAAG AAACTGGTGG 
     1741 ACAGCACCGA CAAGGCCGAC CTGCGGCTGA TCTATCTGGC CCTGGCCCAC ATGATCAAGT 
     1801 TCCGGGGCCA CTTCCTGATC GAGGGCGACC TGAACCCCGA CAACAGCGAC GTGGACAAGC 
     1861 TGTTCATCCA GCTGGTGCAG ACCTACAACC AGCTGTTCGA GGAAAACCCC ATCAACGCCA 
     1921 GCGGCGTGGA CGCCAAGGCC ATCCTGTCTG CCAGACTGAG CAAGAGCAGA CGGCTGGAAA 
     1981 ATCTGATCGC CCAGCTGCCC GGCGAGAAGA AGAATGGCCT GTTCGGCAAC CTGATTGCCC 
     2041 TGAGCCTGGG CCTGACCCCC AACTTCAAGA GCAACTTCGA CCTGGCCGAG GATGCCAAAC 
     2101 TGCAGCTGAG CAAGGACACC TACGACGACG ACCTGGACAA CCTGCTGGCC CAGATCGGCG 
     2161 ACCAGTACGC CGACCTGTTT CTGGCCGCCA AGAACCTGTC CGACGCCATC CTGCTGAGCG 
     2221 ACATCCTGAG AGTGAACACC GAGATCACCA AGGCCCCCCT GAGCGCCTCT ATGATCAAGA 
     2281 GATACGACGA GCACCACCAG GACCTGACCC TGCTGAAAGC TCTCGTGCGG CAGCAGCTGC 
     2341 CTGAGAAGTA CAAAGAGATT TTCTTCGACC AGAGCAAGAA CGGCTACGCC GGCTACATTG 
     2401 ACGGCGGAGC CAGCCAGGAA GAGTTCTACA AGTTCATCAA GCCCATCCTG GAAAAGATGG 
     2461 ACGGCACCGA GGAACTGCTC GTGAAGCTGA ACAGAGAGGA CCTGCTGCGG AAGCAGCGGA 
     2521 CCTTCGACAA CGGCAGCATC CCCCACCAGA TCCACCTGGG AGAGCTGCAC GCCATTCTGC 
     2581 GGCGGCAGGA AGATTTTTAC CCATTCCTGA AGGACAACCG GGAAAAGATC GAGAAGATCC 
     2641 TGACCTTCCG CATCCCCTAC TACGTGGGCC CTCTGGCCAG GGGAAACAGC AGATTCGCCT 
     2701 GGATGACCAG AAAGAGCGAG GAAACCATCA CCCCCTGGAA CTTCGAGGAA GTGGTGGACA 
     2761 AGGGCGCTTC CGCCCAGAGC TTCATCGAGC GGATGACCAA CTTCGATAAG AACCTGCCCA 
     2821 ACGAGAAGGT GCTGCCCAAG CACAGCCTGC TGTACGAGTA CTTCACCGTG TATAACGAGC 
     2881 TGACCAAAGT GAAATACGTG ACCGAGGGAA TGAGAAAGCC CGCCTTCCTG AGCGGCGAGC 
     2941 AGAAAAAGGC CATCGTGGAC CTGCTGTTCA AGACCAACCG GAAAGTGACC GTGAAGCAGC 
     3001 TGAAAGAGGA CTACTTCAAG AAAATCGAGT GCTTCGACTC CGTGGAAATC TCCGGCGTGG 
     3061 AAGATCGGTT CAACGCCTCC CTGGGCACAT ACCACGATCT GCTGAAAATT ATCAAGGACA 
     3121 AGGACTTCCT GGACAATGAG GAAAACGAGG ACATTCTGGA AGATATCGTG CTGACCCTGA 
     3181 CACTGTTTGA GGACAGAGAG ATGATCGAGG AACGGCTGAA AACCTATGCC CACCTGTTCG 
     3241 ACGACAAAGT GATGAAGCAG CTGAAGCGGC GGAGATACAC CGGCTGGGGC AGGCTGAGCC 
     3301 GGAAGCTGAT CAACGGCATC CGGGACAAGC AGTCCGGCAA GACAATCCTG GATTTCCTGA 
     3361 AGTCCGACGG CTTCGCCAAC AGAAACTTCA TGCAGCTGAT CCACGACGAC AGCCTGACCT 
     3421 TTAAAGAGGA CATCCAGAAA GCCCAGGTGT CCGGCCAGGG CGATAGCCTG CACGAGCACA 
     3481 TTGCCAATCT GGCCGGCAGC CCCGCCATTA AGAAGGGCAT CCTGCAGACA GTGAAGGTGG 
     3541 TGGACGAGCT CGTGAAAGTG ATGGGCCGGC ACAAGCCCGA GAACATCGTG ATCGAAATGG 
     3601 CCAGAGAGAA CCAGACCACC CAGAAGGGAC AGAAGAACAG CCGCGAGAGA ATGAAGCGGA 
     3661 TCGAAGAGGG CATCAAAGAG CTGGGCAGCC AGATCCTGAA AGAACACCCC GTGGAAAACA 
     3721 CCCAGCTGCA GAACGAGAAG CTGTACCTGT ACTACCTGCA GAATGGGCGG GATATGTACG 
     3781 TGGACCAGGA ACTGGACATC AACCGGCTGT CCGACTACGA TGTGGACGCC ATCGTGCCTC 
     3841 AGAGCTTTCT GAAGGACGAC TCCATCGACA ACAAGGTGCT GACCAGAAGC GACAAGAACC 
     3901 GGGGCAAGAG CGACAACGTG CCCTCCGAAG AGGTCGTGAA GAAGATGAAG AACTACTGGC 
     3961 GGCAGCTGCT GAACGCCAAG CTGATTACCC AGAGAAAGTT CGACAATCTG ACCAAGGCCG 
     4021 AGAGAGGCGG CCTGAGCGAA CTGGATAAGG CCGGCTTCAT CAAGAGACAG CTGGTGGAAA 
     4081 CCCGGCAGAT CACAAAGCAC GTGGCACAGA TCCTGGACTC CCGGATGAAC ACTAAGTACG 
     4141 ACGAGAATGA CAAGCTGATC CGGGAAGTGA AAGTGATCAC CCTGAAGTCC AAGCTGGTGT 
     4201 CCGATTTCCG GAAGGATTTC CAGTTTTACA AAGTGCGCGA GATCAACAAC TACCACCACG 
     4261 CCCACGACGC CTACCTGAAC GCCGTCGTGG GAACCGCCCT GATCAAAAAG TACCCTAAGC 
     4321 TGGAAAGCGA GTTCGTGTAC GGCGACTACA AGGTGTACGA CGTGCGGAAG ATGATCGCCA 
     4381 AGAGCGAGCA GGAAATCGGC AAGGCTACCG CCAAGTACTT CTTCTACAGC AACATCATGA 
     4441 ACTTTTTCAA GACCGAGATT ACCCTGGCCA ACGGCGAGAT CCGGAAGCGG CCTCTGATCG 
     4501 AGACAAACGG CGAAACCGGG GAGATCGTGT GGGATAAGGG CCGGGATTTT GCCACCGTGC 
     4561 GGAAAGTGCT GAGCATGCCC CAAGTGAATA TCGTGAAAAA GACCGAGGTG CAGACAGGCG 
     4621 GCTTCAGCAA AGAGTCTATC CTGCCCAAGA GGAACAGCGA TAAGCTGATC GCCAGAAAGA 
     4681 AGGACTGGga cCCTAAGAAG TACGGCGGCT TCGACAGCCC CACCGTGGCC TATTCTGTGC 
     4741 TGGTGGTGGC CAAAGTGGAA AAGGGCAAGT CCAAGAAACT GAAGAGTGTG AAAGAGCTGC 
     4801 TGGGGATCAC CATCATGGAA AGAAGCAGCT TCGAGAAGAA TCCCATCGAC TTTCTGGAAG 
     4861 CCAAGGGCTA CAAAGAAGTG AAAAAGGACC TGATCATCAA GCTGCCTAAG TACTCCCTGT 
     4921 TCGAGCTGGA AAACGGCCGG AAGAGAATGC TGGCCTCTGC CGGCGAACTG CAGAAGGGAA 
     4981 ACGAACTGGC CCTGCCCTCC AAATATGTGA ACTTCCTGTA CCTGGCCAGC CACTATGAGA 
     5041 AGCTGAAGGG CTCCCCCGAG GATAATGAGC AGAAACAGCT GTTTGTGGAA CAGCACAAGC 
     5101 ACTACCTGGA CGAGATCATC GAGCAGATCA GCGAGTTCTC CAAGAGAGTG ATCCTGGCCG 
     5161 ACGCTAATCT GGACAAAGTG CTGTCCGCCT ACAACAAGCA CCGGGATAAG CCCATCAGAG 
     5221 AGCAGGCCGA GAATATCATC CACCTGTTTA CCCTGACCAA TCTGGGAGCC CCTGCCGCCT 
     5281 TCAAGTACTT TGACACCACC ATCGACCGGA AGAGGTACAC CAGCACCAAA GAGGTGCTGG 
     5341 ACGCCACCCT GATCCACCAG AGCATCACCG GCCTGTACGA GACACGGATC GACCTGTCTC 
     5401 AGCTGGGAGG CGACAAAAGG CCGGCGGCCA CGAAAAAGGc cggCCAGGGA GGAGGATCCC 
     5461 TGCCCACCTG CAGCTGTCTT GATCGAGTTA TACAAAAAGA CAAAGGCCCA TATTATACAC 
     5521 ACCTTGGGGC AGGACCAAGT GTTGCTGCTG TCAGGGAAAT CATGGAGAAT AGGTATGGTC 
     5581 AAAAAGGAAA CGCAATAAGG ATAGAAATAG TAGTGTACAC CGGTAAAGAA GGGAAAAGCT 
     5641 CTCATGGGTG TCCAATTGCT AAGTGGGTTT TAAGAAGAAG CAGTGATGAA GAAAAAGTTC 
     5701 TTTGTTTGGT CCGGCAGCGT ACAGGCCACC ACTGTCCAAC TGCTGTGATG GTGGTGCTCA 
     5761 TCATGGTGTG GGATGGCATC CCTCTTCCAA TGGCCGACCG GCTATACACA GAGCTCACAG 
     5821 AGAATCTAAA GTCATACAAT GGGCACCCTA CCGACAGAAG ATGCACCCTC AATGAAAATC 
     5881 GTACCTGTAC ATGTCAAGGA ATTGATCCAG AGACTTGTGG AGCTTCATTC TCTTTTGGCT 
     5941 GTTCATGGAG TATGTACTTT AATGGCTGTA AGTTTGGTAG AAGCCCAAGC CCCAGAAGAT 
     6001 TTAGAATTGA TCCAAGCTCT CCCTTACATG AAAAAAACCT TGAAGATAAC TTACAGAGTT 
     6061 TGGCTACACG ATTAGCTCCA ATTTATAAGC AGTATGCTCC AGTAGCTTAC CAAAATCAGG 
     6121 TGGAATATGA AAATGTTGCC CGAGAATGTC GGCTTGGCAG CAAGGAAGGT CGACCCTTCT 
     6181 CTGGGGTCAC TGCTTGCCTG GACTTCTGTG CTCATCCCCA CAGGGACATT CACAACATGA 
     6241 ATAATGGAAG CACTGTGGTT TGTACCTTAA CTCGAGAAGA TAACCGCTCT TTGGGTGTTA 
     6301 TTCCTCAAGA TGAGCAGCTC CATGTGCTAC CTCTTTATAA GCTTTCAGAC ACAGATGAGT 
     6361 TTGGCTCCAA GGAAGGAATG GAAGCCAAGA TCAAATCTGG GGCCATCGAG GTCCTGGCAC 
     6421 CCCGCCGCAA AAAAAGAACG TGTTTCACTC AGCCTGTTCC CCGTTCTGGA AAGAAGAGGG 
     6481 CTGCGATGAT GACAGAGGTT CTTGCACATA AGATAAGGGC AGTGGAAAAG AAACCTATTC 
     6541 CCCGAATCAA GCGGAAGAAT AACTCAACAA CAACAAACAA CAGTAAGCCT TCGTCACTGC 
     6601 CAACCTTAGG GAGTAACACT GAGACCGTGC AACCTGAAGT AAAAAGTGAA ACCGAACCCC 
     6661 ATTTTATCTT AAAAAGTTCA GACAACACTA AAACTTATTC GCTGATGCCA TCCGCTCCTC 
     6721 ACCCAGTGAA AGAGGCATCT CCAGGCTTCT CCTGGTCCCC GAAGACTGCT TCAGCCACAC 
     6781 CAGCTCCACT GAAGAATGAC GCAACAGCCT CATGCGGGTT TTCAGAAAGA AGCAGCACTC 
     6841 CCCACTGTAC GATGCCTTCG GGAAGACTCA GTGGTGCCAA TGCTGCAGCT GCTGATGGCC 
     6901 CTGGCATTTC ACAGCTTGGC GAAGTGGCTC CTCTCCCCAC CCTGTCTGCT CCTGTGATGG 
     6961 AGCCCCTCAT TAATTCTGAG CCTTCCACTG GTGTGACTGA GCCGCTAACG CCTCATCAGC 
     7021 CAAACCACCA GCCCTCCTTC CTCACCTCTC CTCAAGACCT TGCCTCTTCT CCAATGGAAG 
     7081 AAGATGAGCA GCATTCTGAA GCAGATGAGC CTCCATCAGA CGAACCCCTA TCTGATGACC 
     7141 CCCTGTCACC TGCTGAGGAG AAATTGCCCC ACATTGATGA GTATTGGTCA GACAGTGAGC 
     7201 ACATCTTTTT GGATGCAAAT ATTGGTGGGG TGGCCATCGC ACCTGCTCAC GGCTCGGTTT 
     7261 TGATTGAGTG TGCCCGGCGA GAGCTGCACG CTACCACTCC TGTTGAGCAC CCCAACCGTA 
     7321 ATCATCCAAC CCGCCTCTCC CTTGTCTTTT ACCAGCACAA AAACCTAAAT AAGCCCCAAC 
     7381 ATGGTTTTGA ACTAAACAAG ATTAAGTTTG AGGCTAAAGA AGCTAAGAAT AAGAAAATGA 
     7441 AGGCCTCAGA GCAAAAAGAC CAGGCAGCTA ATGAAGGTCC AGAACAGTCC TCTGAAGTAA 
     7501 ATGAATTGAA CCAAATTCCT TCTCATAAAG CATTAACATT AACCCATGAC AATGTTGTCA 
     7561 CCGTGTCCCC TTATGCTCTC ACACACGTTG CGGGGCCCTA TAACCATTGG GTCATGGccg 
     7621 gCCAGGCAAA AAAGAAAAAG gaattcGGCA GTGGAGAGGG CAGAGGAAGT CTGCTAACAT 
     7681 GCGGTGACGT CGAGGAGAAT CCTGGCCCAG TGAGCAAGGG CGAGGAGGAT AACATGGCCA 
     7741 TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT 
     7801 TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA 
     7861 AGGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT 
     7921 ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT 
     7981 TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG 
     8041 TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA 
     8101 CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAAAC CATGGGCTGG GAGGCCTCCT 
     8161 CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC 
     8221 TGAAGGACGG CGGCCACTAC GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG 
     8281 TGCAGCTGCC CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG 
     8341 ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG 
     8401 ACGAGCTGTA CAAGTAAgaa ttctaaCTAG AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC 
     8461 TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC 
     8521 CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG 
     8581 TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGAgAA 
     8641 TAGCAGGCAT GCTGGGGAgc ggccgcagga acccctagtg atggagttgg ccactccctc 
     8701 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt 
     8761 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg 
     8821 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag 
     8881 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 
     8941 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 
     9001 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 
     9061 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg 
     9121 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 
     9181 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta 
     9241 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 
     9301 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc 
     9361 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc 
     9421 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc 
     9481 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg 
     9541 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc 
     9601 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 
     9661 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 
     9721 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 
     9781 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 
     9841 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 
     9901 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 
     9961 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 
    10021 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 
    10081 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 
    10141 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 
    10201 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 
    10261 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 
    10321 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 
    10381 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga 
    10441 agccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 
    10501 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 
    10561 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 
    10621 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 
    10681 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 
    10741 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
    10801 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
    10861 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 
    10921 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
    10981 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
    11041 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
    11101 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
    11161 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
    11221 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
    11281 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
    11341 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
    11401 atgt
//