LOCUS       16-pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-12.xdna    11407 bp      DNA     circular   19.06.2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : 3-pSpdCas9-huTET1CD-T2A-mCherry(PX458).xdna [1
            1378  nt] : (#BbsI[260] / #BbsI[252]) to ExampleSgRNA_clonin
            gHuSg12.xdna [21  nt] : (#BbsI[1] / #BbsI[40])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    1341..1343
                     /label=pUC ori
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="D10A" - label=pUC ori - ApEinfo_fwdcolor=#e8edc7 - ApEinfo_revcolor=#76fffa - 1092..1094 \ 
     misc_feature    1287..1307
                     /label=NLS
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=NLS - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - 1038..1058 \ 
     misc_feature    1341..1343
                     /label=(GAC)D10A(GCC)
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=(GAC)D10A(GCC) - ApEinfo_fwdcolor=#ff0c00 - ApEinfo_revcolor=#ff0c00 - 1092..1094 \ 
     misc_feature    3831..3833
                     /label=(CAT)H840A(GCC)
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=(CAT)H840A(GCC) - ApEinfo_fwdcolor=#ff002a - ApEinfo_revcolor=#ff002a - 3582..3584 \ 
     misc_feature    1257..1283
                     /label=HA-Tag
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=HA-Tag - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - 1008..1034 \ 
     misc_feature    7713..8420
                     /label=mCherry-Mut-BbsI
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    8141..8141
                     /label=Mut-G433A-Lysin
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    8430..8660
                     /label=bGH polyA
                     /SerialCloner_Color=&h408080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=bGH polyA - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 6312..6542 \ 
     rep_origin      10682..11364
                     /label=ColE1 origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=ColE1 origin - ApEinfo_fwdcolor=gray50 - ApEinfo_revcolor=gray50 - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 8564..9246 \ 
     rep_origin      8902..9208
                     /label=F1 ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=F1 ori - ApEinfo_fwdcolor=gray50 - ApEinfo_revcolor=gray50 - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 6784..7090 \ 
     rep_origin      8885..9340
                     /label=M13 origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 origin - ApEinfo_fwdcolor=gray50 - ApEinfo_revcolor=gray50 - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 6767..7222 \ 
     misc_feature    9727..10583
                     /label=AmpR
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=AmpR - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 7609..8465 \ 
     misc_feature    1..248
                     /label=humanU6 promoter
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=humanU6 promoter - ApEinfo_fwdcolor=#0080ff - ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 1..248 \ 
     misc_feature    444..1240
                     /label=CBh promoter
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CBh promoter - ApEinfo_fwdcolor=#f5010a - ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 441..1237 \ 
                     5447..7620
                     /label=insert from PCR(pJFA344C7-Tet1-CD-Plasmid-Addgene49236.xdna)
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from PCR(pJFA344C7-Tet1-CD-Plasmid-Addgene49236.xdna) [2174  nt] : (FseI[5] / FseI[2183]) \ 
     misc_feature    5457..7616
                     /label=Tet1-CD
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6222..6224
                     /label=H not mutated
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6228..6230
                     /label=D not mutated
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    250..270
                     /label=SgRNA12_huTSDR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from ExampleSgRNA_cloningHuSg12.xdna [21  nt] : (#BbsI[1] / #BbsI[40]) \ 
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA 
      241 CGAAAcaccG TCTGAGAAAC CCAGTCAGAA gttttagagc taGAAAtagc aagttaaaat 
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcTTTT TTgttttaga 
      361 gctagaaata gcaagttaaa ataaggctag tccgtTTTTa gcgcgtgcgc caattctgca 
      421 gacaaatggc tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac 
      481 cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac 
      541 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      601 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattGtgccc 
      661 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      721 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc 
      781 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg 
      841 gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc 
      901 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga 
      961 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga 
     1021 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga 
     1081 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat 
     1141 tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt aatgtttaat 
     1201 tacctggagc acctgcctga aatcactttt tttcaggttG GaccggTgcc accATGTACC 
     1261 CATACGATGT TCCAGATTAC GCTTCGCCGA AGAAAAAGCG CAAGGTCGAA GCGTCCGACA 
     1321 AGAAGTACAG CATCGGCCTG GCCATCGGCA CCAACTCTGT GGGCTGGGCC GTGATCACCG 
     1381 ACGAGTACAA GGTGCCCAGC AAGAAATTCA AGGTGCTGGG CAACACCGAC CGGCACAGCA 
     1441 TCAAGAAGAA CCTGATCGGA GCCCTGCTGT TCGACAGCGG CGAAACAGCC GAGGCCACCC 
     1501 GGCTGAAGAG AACCGCCAGA AGAAGATACA CCAGACGGAA GAACCGGATC TGCTATCTGC 
     1561 AAGAGATCTT CAGCAACGAG ATGGCCAAGG TGGACGACAG CTTCTTCCAC AGACTGGAAG 
     1621 AGTCCTTCCT GGTGGAAGAG GATAAGAAGC ACGAGCGGCA CCCCATCTTC GGCAACATCG 
     1681 TGGACGAGGT GGCCTACCAC GAGAAGTACC CCACCATCTA CCACCTGAGA AAGAAACTGG 
     1741 TGGACAGCAC CGACAAGGCC GACCTGCGGC TGATCTATCT GGCCCTGGCC CACATGATCA 
     1801 AGTTCCGGGG CCACTTCCTG ATCGAGGGCG ACCTGAACCC CGACAACAGC GACGTGGACA 
     1861 AGCTGTTCAT CCAGCTGGTG CAGACCTACA ACCAGCTGTT CGAGGAAAAC CCCATCAACG 
     1921 CCAGCGGCGT GGACGCCAAG GCCATCCTGT CTGCCAGACT GAGCAAGAGC AGACGGCTGG 
     1981 AAAATCTGAT CGCCCAGCTG CCCGGCGAGA AGAAGAATGG CCTGTTCGGC AACCTGATTG 
     2041 CCCTGAGCCT GGGCCTGACC CCCAACTTCA AGAGCAACTT CGACCTGGCC GAGGATGCCA 
     2101 AACTGCAGCT GAGCAAGGAC ACCTACGACG ACGACCTGGA CAACCTGCTG GCCCAGATCG 
     2161 GCGACCAGTA CGCCGACCTG TTTCTGGCCG CCAAGAACCT GTCCGACGCC ATCCTGCTGA 
     2221 GCGACATCCT GAGAGTGAAC ACCGAGATCA CCAAGGCCCC CCTGAGCGCC TCTATGATCA 
     2281 AGAGATACGA CGAGCACCAC CAGGACCTGA CCCTGCTGAA AGCTCTCGTG CGGCAGCAGC 
     2341 TGCCTGAGAA GTACAAAGAG ATTTTCTTCG ACCAGAGCAA GAACGGCTAC GCCGGCTACA 
     2401 TTGACGGCGG AGCCAGCCAG GAAGAGTTCT ACAAGTTCAT CAAGCCCATC CTGGAAAAGA 
     2461 TGGACGGCAC CGAGGAACTG CTCGTGAAGC TGAACAGAGA GGACCTGCTG CGGAAGCAGC 
     2521 GGACCTTCGA CAACGGCAGC ATCCCCCACC AGATCCACCT GGGAGAGCTG CACGCCATTC 
     2581 TGCGGCGGCA GGAAGATTTT TACCCATTCC TGAAGGACAA CCGGGAAAAG ATCGAGAAGA 
     2641 TCCTGACCTT CCGCATCCCC TACTACGTGG GCCCTCTGGC CAGGGGAAAC AGCAGATTCG 
     2701 CCTGGATGAC CAGAAAGAGC GAGGAAACCA TCACCCCCTG GAACTTCGAG GAAGTGGTGG 
     2761 ACAAGGGCGC TTCCGCCCAG AGCTTCATCG AGCGGATGAC CAACTTCGAT AAGAACCTGC 
     2821 CCAACGAGAA GGTGCTGCCC AAGCACAGCC TGCTGTACGA GTACTTCACC GTGTATAACG 
     2881 AGCTGACCAA AGTGAAATAC GTGACCGAGG GAATGAGAAA GCCCGCCTTC CTGAGCGGCG 
     2941 AGCAGAAAAA GGCCATCGTG GACCTGCTGT TCAAGACCAA CCGGAAAGTG ACCGTGAAGC 
     3001 AGCTGAAAGA GGACTACTTC AAGAAAATCG AGTGCTTCGA CTCCGTGGAA ATCTCCGGCG 
     3061 TGGAAGATCG GTTCAACGCC TCCCTGGGCA CATACCACGA TCTGCTGAAA ATTATCAAGG 
     3121 ACAAGGACTT CCTGGACAAT GAGGAAAACG AGGACATTCT GGAAGATATC GTGCTGACCC 
     3181 TGACACTGTT TGAGGACAGA GAGATGATCG AGGAACGGCT GAAAACCTAT GCCCACCTGT 
     3241 TCGACGACAA AGTGATGAAG CAGCTGAAGC GGCGGAGATA CACCGGCTGG GGCAGGCTGA 
     3301 GCCGGAAGCT GATCAACGGC ATCCGGGACA AGCAGTCCGG CAAGACAATC CTGGATTTCC 
     3361 TGAAGTCCGA CGGCTTCGCC AACAGAAACT TCATGCAGCT GATCCACGAC GACAGCCTGA 
     3421 CCTTTAAAGA GGACATCCAG AAAGCCCAGG TGTCCGGCCA GGGCGATAGC CTGCACGAGC 
     3481 ACATTGCCAA TCTGGCCGGC AGCCCCGCCA TTAAGAAGGG CATCCTGCAG ACAGTGAAGG 
     3541 TGGTGGACGA GCTCGTGAAA GTGATGGGCC GGCACAAGCC CGAGAACATC GTGATCGAAA 
     3601 TGGCCAGAGA GAACCAGACC ACCCAGAAGG GACAGAAGAA CAGCCGCGAG AGAATGAAGC 
     3661 GGATCGAAGA GGGCATCAAA GAGCTGGGCA GCCAGATCCT GAAAGAACAC CCCGTGGAAA 
     3721 ACACCCAGCT GCAGAACGAG AAGCTGTACC TGTACTACCT GCAGAATGGG CGGGATATGT 
     3781 ACGTGGACCA GGAACTGGAC ATCAACCGGC TGTCCGACTA CGATGTGGAC GCCATCGTGC 
     3841 CTCAGAGCTT TCTGAAGGAC GACTCCATCG ACAACAAGGT GCTGACCAGA AGCGACAAGA 
     3901 ACCGGGGCAA GAGCGACAAC GTGCCCTCCG AAGAGGTCGT GAAGAAGATG AAGAACTACT 
     3961 GGCGGCAGCT GCTGAACGCC AAGCTGATTA CCCAGAGAAA GTTCGACAAT CTGACCAAGG 
     4021 CCGAGAGAGG CGGCCTGAGC GAACTGGATA AGGCCGGCTT CATCAAGAGA CAGCTGGTGG 
     4081 AAACCCGGCA GATCACAAAG CACGTGGCAC AGATCCTGGA CTCCCGGATG AACACTAAGT 
     4141 ACGACGAGAA TGACAAGCTG ATCCGGGAAG TGAAAGTGAT CACCCTGAAG TCCAAGCTGG 
     4201 TGTCCGATTT CCGGAAGGAT TTCCAGTTTT ACAAAGTGCG CGAGATCAAC AACTACCACC 
     4261 ACGCCCACGA CGCCTACCTG AACGCCGTCG TGGGAACCGC CCTGATCAAA AAGTACCCTA 
     4321 AGCTGGAAAG CGAGTTCGTG TACGGCGACT ACAAGGTGTA CGACGTGCGG AAGATGATCG 
     4381 CCAAGAGCGA GCAGGAAATC GGCAAGGCTA CCGCCAAGTA CTTCTTCTAC AGCAACATCA 
     4441 TGAACTTTTT CAAGACCGAG ATTACCCTGG CCAACGGCGA GATCCGGAAG CGGCCTCTGA 
     4501 TCGAGACAAA CGGCGAAACC GGGGAGATCG TGTGGGATAA GGGCCGGGAT TTTGCCACCG 
     4561 TGCGGAAAGT GCTGAGCATG CCCCAAGTGA ATATCGTGAA AAAGACCGAG GTGCAGACAG 
     4621 GCGGCTTCAG CAAAGAGTCT ATCCTGCCCA AGAGGAACAG CGATAAGCTG ATCGCCAGAA 
     4681 AGAAGGACTG GgacCCTAAG AAGTACGGCG GCTTCGACAG CCCCACCGTG GCCTATTCTG 
     4741 TGCTGGTGGT GGCCAAAGTG GAAAAGGGCA AGTCCAAGAA ACTGAAGAGT GTGAAAGAGC 
     4801 TGCTGGGGAT CACCATCATG GAAAGAAGCA GCTTCGAGAA GAATCCCATC GACTTTCTGG 
     4861 AAGCCAAGGG CTACAAAGAA GTGAAAAAGG ACCTGATCAT CAAGCTGCCT AAGTACTCCC 
     4921 TGTTCGAGCT GGAAAACGGC CGGAAGAGAA TGCTGGCCTC TGCCGGCGAA CTGCAGAAGG 
     4981 GAAACGAACT GGCCCTGCCC TCCAAATATG TGAACTTCCT GTACCTGGCC AGCCACTATG 
     5041 AGAAGCTGAA GGGCTCCCCC GAGGATAATG AGCAGAAACA GCTGTTTGTG GAACAGCACA 
     5101 AGCACTACCT GGACGAGATC ATCGAGCAGA TCAGCGAGTT CTCCAAGAGA GTGATCCTGG 
     5161 CCGACGCTAA TCTGGACAAA GTGCTGTCCG CCTACAACAA GCACCGGGAT AAGCCCATCA 
     5221 GAGAGCAGGC CGAGAATATC ATCCACCTGT TTACCCTGAC CAATCTGGGA GCCCCTGCCG 
     5281 CCTTCAAGTA CTTTGACACC ACCATCGACC GGAAGAGGTA CACCAGCACC AAAGAGGTGC 
     5341 TGGACGCCAC CCTGATCCAC CAGAGCATCA CCGGCCTGTA CGAGACACGG ATCGACCTGT 
     5401 CTCAGCTGGG AGGCGACAAA AGGCCGGCGG CCACGAAAAA GGccggCCAG GGAGGAGGAT 
     5461 CCCTGCCCAC CTGCAGCTGT CTTGATCGAG TTATACAAAA AGACAAAGGC CCATATTATA 
     5521 CACACCTTGG GGCAGGACCA AGTGTTGCTG CTGTCAGGGA AATCATGGAG AATAGGTATG 
     5581 GTCAAAAAGG AAACGCAATA AGGATAGAAA TAGTAGTGTA CACCGGTAAA GAAGGGAAAA 
     5641 GCTCTCATGG GTGTCCAATT GCTAAGTGGG TTTTAAGAAG AAGCAGTGAT GAAGAAAAAG 
     5701 TTCTTTGTTT GGTCCGGCAG CGTACAGGCC ACCACTGTCC AACTGCTGTG ATGGTGGTGC 
     5761 TCATCATGGT GTGGGATGGC ATCCCTCTTC CAATGGCCGA CCGGCTATAC ACAGAGCTCA 
     5821 CAGAGAATCT AAAGTCATAC AATGGGCACC CTACCGACAG AAGATGCACC CTCAATGAAA 
     5881 ATCGTACCTG TACATGTCAA GGAATTGATC CAGAGACTTG TGGAGCTTCA TTCTCTTTTG 
     5941 GCTGTTCATG GAGTATGTAC TTTAATGGCT GTAAGTTTGG TAGAAGCCCA AGCCCCAGAA 
     6001 GATTTAGAAT TGATCCAAGC TCTCCCTTAC ATGAAAAAAA CCTTGAAGAT AACTTACAGA 
     6061 GTTTGGCTAC ACGATTAGCT CCAATTTATA AGCAGTATGC TCCAGTAGCT TACCAAAATC 
     6121 AGGTGGAATA TGAAAATGTT GCCCGAGAAT GTCGGCTTGG CAGCAAGGAA GGTCGACCCT 
     6181 TCTCTGGGGT CACTGCTTGC CTGGACTTCT GTGCTCATCC CCACAGGGAC ATTCACAACA 
     6241 TGAATAATGG AAGCACTGTG GTTTGTACCT TAACTCGAGA AGATAACCGC TCTTTGGGTG 
     6301 TTATTCCTCA AGATGAGCAG CTCCATGTGC TACCTCTTTA TAAGCTTTCA GACACAGATG 
     6361 AGTTTGGCTC CAAGGAAGGA ATGGAAGCCA AGATCAAATC TGGGGCCATC GAGGTCCTGG 
     6421 CACCCCGCCG CAAAAAAAGA ACGTGTTTCA CTCAGCCTGT TCCCCGTTCT GGAAAGAAGA 
     6481 GGGCTGCGAT GATGACAGAG GTTCTTGCAC ATAAGATAAG GGCAGTGGAA AAGAAACCTA 
     6541 TTCCCCGAAT CAAGCGGAAG AATAACTCAA CAACAACAAA CAACAGTAAG CCTTCGTCAC 
     6601 TGCCAACCTT AGGGAGTAAC ACTGAGACCG TGCAACCTGA AGTAAAAAGT GAAACCGAAC 
     6661 CCCATTTTAT CTTAAAAAGT TCAGACAACA CTAAAACTTA TTCGCTGATG CCATCCGCTC 
     6721 CTCACCCAGT GAAAGAGGCA TCTCCAGGCT TCTCCTGGTC CCCGAAGACT GCTTCAGCCA 
     6781 CACCAGCTCC ACTGAAGAAT GACGCAACAG CCTCATGCGG GTTTTCAGAA AGAAGCAGCA 
     6841 CTCCCCACTG TACGATGCCT TCGGGAAGAC TCAGTGGTGC CAATGCTGCA GCTGCTGATG 
     6901 GCCCTGGCAT TTCACAGCTT GGCGAAGTGG CTCCTCTCCC CACCCTGTCT GCTCCTGTGA 
     6961 TGGAGCCCCT CATTAATTCT GAGCCTTCCA CTGGTGTGAC TGAGCCGCTA ACGCCTCATC 
     7021 AGCCAAACCA CCAGCCCTCC TTCCTCACCT CTCCTCAAGA CCTTGCCTCT TCTCCAATGG 
     7081 AAGAAGATGA GCAGCATTCT GAAGCAGATG AGCCTCCATC AGACGAACCC CTATCTGATG 
     7141 ACCCCCTGTC ACCTGCTGAG GAGAAATTGC CCCACATTGA TGAGTATTGG TCAGACAGTG 
     7201 AGCACATCTT TTTGGATGCA AATATTGGTG GGGTGGCCAT CGCACCTGCT CACGGCTCGG 
     7261 TTTTGATTGA GTGTGCCCGG CGAGAGCTGC ACGCTACCAC TCCTGTTGAG CACCCCAACC 
     7321 GTAATCATCC AACCCGCCTC TCCCTTGTCT TTTACCAGCA CAAAAACCTA AATAAGCCCC 
     7381 AACATGGTTT TGAACTAAAC AAGATTAAGT TTGAGGCTAA AGAAGCTAAG AATAAGAAAA 
     7441 TGAAGGCCTC AGAGCAAAAA GACCAGGCAG CTAATGAAGG TCCAGAACAG TCCTCTGAAG 
     7501 TAAATGAATT GAACCAAATT CCTTCTCATA AAGCATTAAC ATTAACCCAT GACAATGTTG 
     7561 TCACCGTGTC CCCTTATGCT CTCACACACG TTGCGGGGCC CTATAACCAT TGGGTCATGG 
     7621 ccggCCAGGC AAAAAAGAAA AAGgaattcG GCAGTGGAGA GGGCAGAGGA AGTCTGCTAA 
     7681 CATGCGGTGA CGTCGAGGAG AATCCTGGCC CAGTGAGCAA GGGCGAGGAG GATAACATGG 
     7741 CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA GGGCTCCGTG AACGGCCACG 
     7801 AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGGCACCCAG ACCGCCAAGC 
     7861 TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA CATCCTGTCC CCTCAGTTCA 
     7921 TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT CCCCGACTAC TTGAAGCTGT 
     7981 CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT CGAGGACGGC GGCGTGGTGA 
     8041 CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT CTACAAGGTG AAGCTGCGCG 
     8101 GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA AACCATGGGC TGGGAGGCCT 
     8161 CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG CGAGATCAAG CAGAGGCTGA 
     8221 AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC CACCTACAAG GCCAAGAAGC 
     8281 CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC TCCCACAACG 
     8341 AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC ACCGGCGGCA 
     8401 TGGACGAGCT GTACAAGTAA gaattctaaC TAGAGCTCGC TGATCAGCCT CGACTGTGCC 
     8461 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG 
     8521 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG 
     8581 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA 
     8641 gAATAGCAGG CATGCTGGGG Agcggccgca ggaaccccta gtgatggagt tggccactcc 
     8701 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg 
     8761 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat 
     8821 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca 
     8881 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 
     8941 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 
     9001 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 
     9061 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt 
     9121 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 
     9181 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat 
     9241 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 
     9301 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca 
     9361 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg 
     9421 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg 
     9481 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc 
     9541 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt 
     9601 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 
     9661 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 
     9721 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 
     9781 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 
     9841 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 
     9901 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 
     9961 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 
    10021 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 
    10081 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 
    10141 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 
    10201 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 
    10261 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 
    10321 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 
    10381 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 
    10441 ggaagccgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 
    10501 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 
    10561 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 
    10621 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 
    10681 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 
    10741 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 
    10801 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 
    10861 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg 
    10921 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 
    10981 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 
    11041 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 
    11101 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 
    11161 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 
    11221 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 
    11281 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 
    11341 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 
    11401 cacatgt
//