LOCUS       19-pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-3.xdna    11406 bp      DNA     circular   19.06.2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : 4-pSpdCas9-hudTET1CD-T2A-mCherry(PX458).xdna [
            11378  nt] : (#BbsI[260] / #BbsI[252]) to ExampleSgRNA_cloni
            ngHuSg3.xdna [20  nt] : (#BbsI[1] / #BbsI[39])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    1340..1342
                     /label=pUC ori
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="D10A" - label=pUC ori - ApEinfo_fwdcolor=#e8edc7 - ApEinfo_revcolor=#76fffa - 1092..1094 \ 
     misc_feature    1286..1306
                     /label=NLS
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=NLS - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - 1038..1058 \ 
     misc_feature    1340..1342
                     /label=(GAC)D10A(GCC)
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=(GAC)D10A(GCC) - ApEinfo_fwdcolor=#ff0c00 - ApEinfo_revcolor=#ff0c00 - 1092..1094 \ 
     misc_feature    3830..3832
                     /label=(CAT)H840A(GCC)
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=(CAT)H840A(GCC) - ApEinfo_fwdcolor=#ff002a - ApEinfo_revcolor=#ff002a - 3582..3584 \ 
     misc_feature    1256..1282
                     /label=HA-Tag
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=HA-Tag - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - 1008..1034 \ 
     misc_feature    7712..8419
                     /label=mCherry-Mut-BbsI
                     /SerialCloner_Color=&hFF00FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    8140..8140
                     /label=Mut-G433A-Lysin
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    8429..8659
                     /label=bGH polyA
                     /SerialCloner_Color=&h408080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=bGH polyA - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 6312..6542 \ 
     rep_origin      10681..11363
                     /label=ColE1 origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=ColE1 origin - ApEinfo_fwdcolor=gray50 - ApEinfo_revcolor=gray50 - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 8564..9246 \ 
     rep_origin      8901..9207
                     /label=F1 ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=F1 ori - ApEinfo_fwdcolor=gray50 - ApEinfo_revcolor=gray50 - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 6784..7090 \ 
     rep_origin      8884..9339
                     /label=M13 origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 origin - ApEinfo_fwdcolor=gray50 - ApEinfo_revcolor=gray50 - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 6767..7222 \ 
     misc_feature    9726..10582
                     /label=AmpR
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=AmpR - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 7609..8465 \ 
     misc_feature    1..248
                     /label=humanU6 promoter
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=humanU6 promoter - ApEinfo_fwdcolor=#0080ff - ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 1..248 \ 
     misc_feature    443..1239
                     /label=CBh promoter
                     /SerialCloner_Color=&h0080FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CBh promoter - ApEinfo_fwdcolor=#f5010a - ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 441..1237 \ 
     misc_feature    5456..7615
                     /label=MUTATED-TED1-CD
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6221..6223
                     /label=H1671Y mut
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6227..6229
                     /label=D1673A mut
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    250..269
                     /label=SgRNA3_huTSDR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from ExampleSgRNA_cloningHuSg3.xdna [20  nt] : (#BbsI[1] / #BbsI[39]) \ 
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA 
      241 CGAAAcaccG GGCCAGGTCT TCAGAGCTAg ttttagagct aGAAAtagca agttaaaata 
      301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcTTTTT Tgttttagag 
      361 ctagaaatag caagttaaaa taaggctagt ccgtTTTTag cgcgtgcgcc aattctgcag 
      421 acaaatggct ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc 
      481 gcccaacgac ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg 
      541 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 
      601 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attGtgccca 
      661 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 
      721 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc 
      781 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg 
      841 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg 
      901 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag 
      961 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgac 
     1021 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac 
     1081 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt 
     1141 agctgagcaa gaggtaaggg tttaagggat ggttggttgg tggggtatta atgtttaatt 
     1201 acctggagca cctgcctgaa atcacttttt ttcaggttGG accggTgcca ccATGTACCC 
     1261 ATACGATGTT CCAGATTACG CTTCGCCGAA GAAAAAGCGC AAGGTCGAAG CGTCCGACAA 
     1321 GAAGTACAGC ATCGGCCTGG CCATCGGCAC CAACTCTGTG GGCTGGGCCG TGATCACCGA 
     1381 CGAGTACAAG GTGCCCAGCA AGAAATTCAA GGTGCTGGGC AACACCGACC GGCACAGCAT 
     1441 CAAGAAGAAC CTGATCGGAG CCCTGCTGTT CGACAGCGGC GAAACAGCCG AGGCCACCCG 
     1501 GCTGAAGAGA ACCGCCAGAA GAAGATACAC CAGACGGAAG AACCGGATCT GCTATCTGCA 
     1561 AGAGATCTTC AGCAACGAGA TGGCCAAGGT GGACGACAGC TTCTTCCACA GACTGGAAGA 
     1621 GTCCTTCCTG GTGGAAGAGG ATAAGAAGCA CGAGCGGCAC CCCATCTTCG GCAACATCGT 
     1681 GGACGAGGTG GCCTACCACG AGAAGTACCC CACCATCTAC CACCTGAGAA AGAAACTGGT 
     1741 GGACAGCACC GACAAGGCCG ACCTGCGGCT GATCTATCTG GCCCTGGCCC ACATGATCAA 
     1801 GTTCCGGGGC CACTTCCTGA TCGAGGGCGA CCTGAACCCC GACAACAGCG ACGTGGACAA 
     1861 GCTGTTCATC CAGCTGGTGC AGACCTACAA CCAGCTGTTC GAGGAAAACC CCATCAACGC 
     1921 CAGCGGCGTG GACGCCAAGG CCATCCTGTC TGCCAGACTG AGCAAGAGCA GACGGCTGGA 
     1981 AAATCTGATC GCCCAGCTGC CCGGCGAGAA GAAGAATGGC CTGTTCGGCA ACCTGATTGC 
     2041 CCTGAGCCTG GGCCTGACCC CCAACTTCAA GAGCAACTTC GACCTGGCCG AGGATGCCAA 
     2101 ACTGCAGCTG AGCAAGGACA CCTACGACGA CGACCTGGAC AACCTGCTGG CCCAGATCGG 
     2161 CGACCAGTAC GCCGACCTGT TTCTGGCCGC CAAGAACCTG TCCGACGCCA TCCTGCTGAG 
     2221 CGACATCCTG AGAGTGAACA CCGAGATCAC CAAGGCCCCC CTGAGCGCCT CTATGATCAA 
     2281 GAGATACGAC GAGCACCACC AGGACCTGAC CCTGCTGAAA GCTCTCGTGC GGCAGCAGCT 
     2341 GCCTGAGAAG TACAAAGAGA TTTTCTTCGA CCAGAGCAAG AACGGCTACG CCGGCTACAT 
     2401 TGACGGCGGA GCCAGCCAGG AAGAGTTCTA CAAGTTCATC AAGCCCATCC TGGAAAAGAT 
     2461 GGACGGCACC GAGGAACTGC TCGTGAAGCT GAACAGAGAG GACCTGCTGC GGAAGCAGCG 
     2521 GACCTTCGAC AACGGCAGCA TCCCCCACCA GATCCACCTG GGAGAGCTGC ACGCCATTCT 
     2581 GCGGCGGCAG GAAGATTTTT ACCCATTCCT GAAGGACAAC CGGGAAAAGA TCGAGAAGAT 
     2641 CCTGACCTTC CGCATCCCCT ACTACGTGGG CCCTCTGGCC AGGGGAAACA GCAGATTCGC 
     2701 CTGGATGACC AGAAAGAGCG AGGAAACCAT CACCCCCTGG AACTTCGAGG AAGTGGTGGA 
     2761 CAAGGGCGCT TCCGCCCAGA GCTTCATCGA GCGGATGACC AACTTCGATA AGAACCTGCC 
     2821 CAACGAGAAG GTGCTGCCCA AGCACAGCCT GCTGTACGAG TACTTCACCG TGTATAACGA 
     2881 GCTGACCAAA GTGAAATACG TGACCGAGGG AATGAGAAAG CCCGCCTTCC TGAGCGGCGA 
     2941 GCAGAAAAAG GCCATCGTGG ACCTGCTGTT CAAGACCAAC CGGAAAGTGA CCGTGAAGCA 
     3001 GCTGAAAGAG GACTACTTCA AGAAAATCGA GTGCTTCGAC TCCGTGGAAA TCTCCGGCGT 
     3061 GGAAGATCGG TTCAACGCCT CCCTGGGCAC ATACCACGAT CTGCTGAAAA TTATCAAGGA 
     3121 CAAGGACTTC CTGGACAATG AGGAAAACGA GGACATTCTG GAAGATATCG TGCTGACCCT 
     3181 GACACTGTTT GAGGACAGAG AGATGATCGA GGAACGGCTG AAAACCTATG CCCACCTGTT 
     3241 CGACGACAAA GTGATGAAGC AGCTGAAGCG GCGGAGATAC ACCGGCTGGG GCAGGCTGAG 
     3301 CCGGAAGCTG ATCAACGGCA TCCGGGACAA GCAGTCCGGC AAGACAATCC TGGATTTCCT 
     3361 GAAGTCCGAC GGCTTCGCCA ACAGAAACTT CATGCAGCTG ATCCACGACG ACAGCCTGAC 
     3421 CTTTAAAGAG GACATCCAGA AAGCCCAGGT GTCCGGCCAG GGCGATAGCC TGCACGAGCA 
     3481 CATTGCCAAT CTGGCCGGCA GCCCCGCCAT TAAGAAGGGC ATCCTGCAGA CAGTGAAGGT 
     3541 GGTGGACGAG CTCGTGAAAG TGATGGGCCG GCACAAGCCC GAGAACATCG TGATCGAAAT 
     3601 GGCCAGAGAG AACCAGACCA CCCAGAAGGG ACAGAAGAAC AGCCGCGAGA GAATGAAGCG 
     3661 GATCGAAGAG GGCATCAAAG AGCTGGGCAG CCAGATCCTG AAAGAACACC CCGTGGAAAA 
     3721 CACCCAGCTG CAGAACGAGA AGCTGTACCT GTACTACCTG CAGAATGGGC GGGATATGTA 
     3781 CGTGGACCAG GAACTGGACA TCAACCGGCT GTCCGACTAC GATGTGGACG CCATCGTGCC 
     3841 TCAGAGCTTT CTGAAGGACG ACTCCATCGA CAACAAGGTG CTGACCAGAA GCGACAAGAA 
     3901 CCGGGGCAAG AGCGACAACG TGCCCTCCGA AGAGGTCGTG AAGAAGATGA AGAACTACTG 
     3961 GCGGCAGCTG CTGAACGCCA AGCTGATTAC CCAGAGAAAG TTCGACAATC TGACCAAGGC 
     4021 CGAGAGAGGC GGCCTGAGCG AACTGGATAA GGCCGGCTTC ATCAAGAGAC AGCTGGTGGA 
     4081 AACCCGGCAG ATCACAAAGC ACGTGGCACA GATCCTGGAC TCCCGGATGA ACACTAAGTA 
     4141 CGACGAGAAT GACAAGCTGA TCCGGGAAGT GAAAGTGATC ACCCTGAAGT CCAAGCTGGT 
     4201 GTCCGATTTC CGGAAGGATT TCCAGTTTTA CAAAGTGCGC GAGATCAACA ACTACCACCA 
     4261 CGCCCACGAC GCCTACCTGA ACGCCGTCGT GGGAACCGCC CTGATCAAAA AGTACCCTAA 
     4321 GCTGGAAAGC GAGTTCGTGT ACGGCGACTA CAAGGTGTAC GACGTGCGGA AGATGATCGC 
     4381 CAAGAGCGAG CAGGAAATCG GCAAGGCTAC CGCCAAGTAC TTCTTCTACA GCAACATCAT 
     4441 GAACTTTTTC AAGACCGAGA TTACCCTGGC CAACGGCGAG ATCCGGAAGC GGCCTCTGAT 
     4501 CGAGACAAAC GGCGAAACCG GGGAGATCGT GTGGGATAAG GGCCGGGATT TTGCCACCGT 
     4561 GCGGAAAGTG CTGAGCATGC CCCAAGTGAA TATCGTGAAA AAGACCGAGG TGCAGACAGG 
     4621 CGGCTTCAGC AAAGAGTCTA TCCTGCCCAA GAGGAACAGC GATAAGCTGA TCGCCAGAAA 
     4681 GAAGGACTGG gacCCTAAGA AGTACGGCGG CTTCGACAGC CCCACCGTGG CCTATTCTGT 
     4741 GCTGGTGGTG GCCAAAGTGG AAAAGGGCAA GTCCAAGAAA CTGAAGAGTG TGAAAGAGCT 
     4801 GCTGGGGATC ACCATCATGG AAAGAAGCAG CTTCGAGAAG AATCCCATCG ACTTTCTGGA 
     4861 AGCCAAGGGC TACAAAGAAG TGAAAAAGGA CCTGATCATC AAGCTGCCTA AGTACTCCCT 
     4921 GTTCGAGCTG GAAAACGGCC GGAAGAGAAT GCTGGCCTCT GCCGGCGAAC TGCAGAAGGG 
     4981 AAACGAACTG GCCCTGCCCT CCAAATATGT GAACTTCCTG TACCTGGCCA GCCACTATGA 
     5041 GAAGCTGAAG GGCTCCCCCG AGGATAATGA GCAGAAACAG CTGTTTGTGG AACAGCACAA 
     5101 GCACTACCTG GACGAGATCA TCGAGCAGAT CAGCGAGTTC TCCAAGAGAG TGATCCTGGC 
     5161 CGACGCTAAT CTGGACAAAG TGCTGTCCGC CTACAACAAG CACCGGGATA AGCCCATCAG 
     5221 AGAGCAGGCC GAGAATATCA TCCACCTGTT TACCCTGACC AATCTGGGAG CCCCTGCCGC 
     5281 CTTCAAGTAC TTTGACACCA CCATCGACCG GAAGAGGTAC ACCAGCACCA AAGAGGTGCT 
     5341 GGACGCCACC CTGATCCACC AGAGCATCAC CGGCCTGTAC GAGACACGGA TCGACCTGTC 
     5401 TCAGCTGGGA GGCGACAAAA GGCCGGCGGC CACGAAAAAG GccggCCAGG GAGGAGGATC 
     5461 CCTGCCCACC TGCAGCTGTC TTGATCGAGT TATACAAAAA GACAAAGGCC CATATTATAC 
     5521 ACACCTTGGG GCAGGACCAA GTGTTGCTGC TGTCAGGGAA ATCATGGAGA ATAGGTATGG 
     5581 TCAAAAAGGA AACGCAATAA GGATAGAAAT AGTAGTGTAC ACCGGTAAAG AAGGGAAAAG 
     5641 CTCTCATGGG TGTCCAATTG CTAAGTGGGT TTTAAGAAGA AGCAGTGATG AAGAAAAAGT 
     5701 TCTTTGTTTG GTCCGGCAGC GTACAGGCCA CCACTGTCCA ACTGCTGTGA TGGTGGTGCT 
     5761 CATCATGGTG TGGGATGGCA TCCCTCTTCC AATGGCCGAC CGGCTATACA CAGAGCTCAC 
     5821 AGAGAATCTA AAGTCATACA ATGGGCACCC TACCGACAGA AGATGCACCC TCAATGAAAA 
     5881 TCGTACCTGT ACATGTCAAG GAATTGATCC AGAGACTTGT GGAGCTTCAT TCTCTTTTGG 
     5941 CTGTTCATGG AGTATGTACT TTAATGGCTG TAAGTTTGGT AGAAGCCCAA GCCCCAGAAG 
     6001 ATTTAGAATT GATCCAAGCT CTCCCTTACA TGAAAAAAAC CTTGAAGATA ACTTACAGAG 
     6061 TTTGGCTACA CGATTAGCTC CAATTTATAA GCAGTATGCT CCAGTAGCTT ACCAAAATCA 
     6121 GGTGGAATAT GAAAATGTTG CCCGAGAATG TCGGCTTGGC AGCAAGGAAG GTcgACCCTT 
     6181 CTCTGGGGTC ACTGCTTGCC TGGACTTCTG TGCTCATCCC TACAGGGCCA TTCACAACAT 
     6241 GAATAATGGA AGCACTGTGG TTTGTACCTT AACtcgaGAA GATAACCGCT CTTTGGGTGT 
     6301 TATTCCTCAA GATGAGCAGC TCCATGTGCT ACCTCTTTAT AAGCTTTCAG ACACAGATGA 
     6361 GTTTGGCTCC AAGGAAGGAA TGGAAGCCAA GATCAAATCT GGGGCCATCG AGGTCCTGGC 
     6421 ACCCCGCCGC AAAAAAAGAA CGTGTTTCAC TCAGCCTGTT CCCCGTTCTG GAAAGAAGAG 
     6481 GGCTGCGATG ATGACAGAGG TTCTTGCACA TAAGATAAGG GCAGTGGAAA AGAAACCTAT 
     6541 TCCCCGAATC AAGCGGAAGA ATAACTCAAC AACAACAAAC AACAGTAAGC CTTCGTCACT 
     6601 GCCAACCTTA GGGAGTAACA CTGAGACCGT GCAACCTGAA GTAAAAAGTG AAACCGAACC 
     6661 CCATTTTATC TTAAAAAGTT CAGACAACAC TAAAACTTAT TCGCTGATGC CATCCGCTCC 
     6721 TCACCCAGTG AAAGAGGCAT CTCCAGGCTT CTCCTGGTCC CCGAAGACTG CTTCAGCCAC 
     6781 ACCAGCTCCA CTGAAGAATG ACGCAACAGC CTCATGCGGG TTTTCAGAAA GAAGCAGCAC 
     6841 TCCCCACTGT ACGATGCCTT CGGGAAGACT CAGTGGTGCC AATGCTGCAG CTGCTGATGG 
     6901 CCCTGGCATT TCACAGCTTG GCGAAGTGGC TCCTCTCCCC ACCCTGTCTG CTCCTGTGAT 
     6961 GGAGCCCCTC ATTAATTCTG AGCCTTCCAC TGGTGTGACT GAGCCGCTAA CGCCTCATCA 
     7021 GCCAAACCAC CAGCCCTCCT TCCTCACCTC TCCTCAAGAC CTTGCCTCTT CTCCAATGGA 
     7081 AGAAGATGAG CAGCATTCTG AAGCAGATGA GCCTCCATCA GACGAACCCC TATCTGATGA 
     7141 CCCCCTGTCA CCTGCTGAGG AGAAATTGCC CCACATTGAT GAGTATTGGT CAGACAGTGA 
     7201 GCACATCTTT TTGGATGCAA ATATTGGTGG GGTGGCCATC GCACCTGCTC ACGGCTCGGT 
     7261 TTTGATTGAG TGTGCCCGGC GAGAGCTGCA CGCTACCACT CCTGTTGAGC ACCCCAACCG 
     7321 TAATCATCCA ACCCGCCTCT CCCTTGTCTT TTACCAGCAC AAAAACCTAA ATAAGCCCCA 
     7381 ACATGGTTTT GAACTAAACA AGATTAAGTT TGAGGCTAAA GAAGCTAAGA ATAAGAAAAT 
     7441 GAAGGCCTCA GAGCAAAAAG ACCAGGCAGC TAATGAAGGT CCAGAACAGT CCTCTGAAGT 
     7501 AAATGAATTG AACCAAATTC CTTCTCATAA AGCATTAACA TTAACCCATG ACAATGTTGT 
     7561 CACCGTGTCC CCTTATGCTC TCACACACGT TGCGGGGCCC TATAACCATT GGGTCATGGc 
     7621 cggCCAGGCA AAAAAGAAAA AGgaattcGG CAGTGGAGAG GGCAGAGGAA GTCTGCTAAC 
     7681 ATGCGGTGAC GTCGAGGAGA ATCCTGGCCC AGTGAGCAAG GGCGAGGAGG ATAACATGGC 
     7741 CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA 
     7801 GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT 
     7861 GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT 
     7921 GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC 
     7981 CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC 
     8041 CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG 
     8101 CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAA ACCATGGGCT GGGAGGCCTC 
     8161 CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA 
     8221 GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC 
     8281 CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA 
     8341 GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT 
     8401 GGACGAGCTG TACAAGTAAg aattctaaCT AGAGCTCGCT GATCAGCCTC GACTGTGCCT 
     8461 TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT 
     8521 GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG 
     8581 TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAg 
     8641 AATAGCAGGC ATGCTGGGGA gcggccgcag gaacccctag tgatggagtt ggccactccc 
     8701 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc 
     8761 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg 
     8821 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat 
     8881 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     8941 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     9001 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     9061 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg 
     9121 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     9181 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt 
     9241 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     9301 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa 
     9361 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 
     9421 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 
     9481 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 
     9541 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 
     9601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     9661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
     9721 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 
     9781 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 
     9841 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 
     9901 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 
     9961 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 
    10021 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 
    10081 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 
    10141 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 
    10201 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 
    10261 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 
    10321 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 
    10381 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 
    10441 gaagccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 
    10501 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 
    10561 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 
    10621 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 
    10681 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 
    10741 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 
    10801 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 
    10861 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 
    10921 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 
    10981 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 
    11041 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 
    11101 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 
    11161 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 
    11221 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 
    11281 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 
    11341 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 
    11401 acatgt
//