LOCUS       Exported                5448 bp ds-DNA     circular SYN 20-OCT-2011
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 21, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
COMMENT     Full length of U1-C was amplified by 
            PCRusingprimersEcoU1-C001fwd(1469) and 
            SalstopU1-C-end-rev(1472)andinserted intoEcoRI and SalI sites of 
            pGEX-5X-1 plasmid
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_binding    215..237
                     /label=LacO
     misc_feature    259..941
                     /label=GST
     misc_feature    942..947
                     /label=EcoRI
     misc_feature    948..1427
                     /label=U1 RNP-C full length
     misc_feature    948..973
                     /label=Eco001U1-Cfwd
     misc_feature    1404..1427
                     /label=SalstopU1-Crev
     misc_feature    1428..1433
                     /label=SalI
     CDS             2054..2713
                     /label=AmpR
     rep_origin      2811..3493
                     /label=ColE1 origin
     misc_binding    4962..4984
                     /label=LacO
                     /label=LacO(1)
     primer_bind     4990..5010
                     /label=M13-rev
     primer_bind     complement(5022..5039)
                     /label=M13-fwd
     CDS             5110..5178
                     /label=LacZ alpha
ORIGIN
        1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatctg atcgaaggtc gtgggatccc cgaattcatg cccaagtttt
      961 attgtgacta ctgcgataca tacctcaccc atgactctcc atctgtgaga aagacacact
     1021 gcagtggaag gaaacacaaa gagaatgtga aagactatta tcagaaatgg atggaagagc
     1081 aggctcagag cctgattgac aaaacaacgg ctgcatttca acaaggaaag atacctccta
     1141 ctccattctc tgctcctcct cctgcagggg cgatgatacc acctcccccc agccttccgg
     1201 gtcctcctcg ccctggtatg atgcccgcac cccatatggg gggccctccc atgatgccaa
     1261 tgatgggccc tcctcctcct gggatgatgc cagtgggacc tgctcctgga atgaggccgc
     1321 ccatgggagg ccatatgcca atgatgcctg ggcccccaat gatgagacct cctgcccgtc
     1381 ccatgatggt gcccactcgg cccggaatga ctcgaccaga cagataagtc gactcgagcg
     1441 gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     1501 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     1561 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     1621 gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg tgatacgcct
     1681 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     1741 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1801 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1861 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1921 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1981 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     2041 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     2101 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2161 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2221 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2281 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2341 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     2401 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2461 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2521 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     2581 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2641 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2701 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2761 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2821 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2881 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2941 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3001 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3061 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3121 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3181 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3241 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3301 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3361 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3421 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3481 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3541 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3601 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3661 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca taaattccga
     3721 caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
     3781 attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
     3841 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
     3901 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
     3961 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
     4021 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
     4081 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
     4141 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
     4201 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
     4261 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
     4321 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
     4381 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
     4441 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
     4501 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
     4561 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
     4621 taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct
     4681 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
     4741 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca
     4801 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     4861 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     4921 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     4981 aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt cgttttacaa
     5041 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     5101 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     5161 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
     5221 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
     5281 cacggttacg atgcgcccat ctacaccaac gtaacctatc ccattacggt caatccgccg
     5341 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
     5401 tggctacagg aaggccagac gcgaattatt tttgatggcg ttggaatt
//
