LOCUS       HR180-LGR5-GFP               9701 bp ds-DNA     circular     04-JUN-2019
DEFINITION  .
ACCESSION   .
VERSION     .
SOURCE      Human
 ORGANISM   
REFERENCE   1  (bases 402 to 7440)
 AUTHORS    Shimokawa M, Ohta Y, Nishikori S, Matano M, Takano A, Fujii M, Date S, Sugimoto S, Kanai T & Sato T
 TITLE      Visualization and targeting of LGR5+ human colon cancer stem cells
 JOURNAL    Nature. 2017 May 11;545(7653):187-192. doi: 10.1038/nature22081. Epub 2017 Mar 29.
 PUBMED     28355176     
SOURCE      Human
  ORGANISM  
COMMENT     .
FEATURES             Location/Qualifiers
     gene            402..1419
                     /label=LGR5 5' homologous arm
     misc_feature    1462..2075
                     /label=IRES
     misc_feature    2076..2081
                     /label=Kozak
     CDS             2082..2801
                     /label=EGFP
     polyA_signal    2802..3249
                     /label=SV40 PolyA
     misc_feature    3270..3303
                     /label=>LoxP
     enhancer        3367..3598
                     /label=core insulator
     promoter        3625..4170
                     /label=EF1 promoter
     CDS             4183..4860
                     /label=mRuby
     CDS             4861..4914
                     /label=T2A
     CDS             4915..5514
                     /label=Puro
     polyA_signal    5638..5768
                     /label=SV40 pA
     enhancer        complement(6016..6246)
                     /label=core insulator
     misc_feature    6259..6292
                     /label=>LoxP
     misc_feature    6322..7467
                     /label=MCS2
     misc_feature    complement(7867..8481)
                     /label=Rep (pMB1)
     CDS             complement(8641..9501)
                     /label=Amp
     gene            6335..7440
                     /label=LGR5 3' homologous arm
BASE COUNT     2352 a   2556 c   2449 g   2344 t
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CTCTGGCACT TACTTGTAAT
      421 GCTTTGGTGA CTTCAACAGT TTTCAGATCC CCTCTGTACA TTTCCCCCAT TAAACTGTTA
      481 ATTGGGGTCA TCGCAGCAGT GAACATGCTC ACGGGAGTCT CCAGTGCCGT GCTGGCTGGT
      541 GTGGATGCGT TCACTTTTGG CAGCTTTGCA CGACATGGTG CCTGGTGGGA GAATGGGGTT
      601 GGTTGCCATG TCATTGGTTT TTTGTCCATT TTTGCTTCAG AATCATCTGT TTTCCTGCTT
      661 ACTCTGGCAG CCCTGGAGCG TGGGTTCTCT GTGAAATATT CTGCAAAATT TGAAACGAAA
      721 GCTCCATTTT CTAGCCTGAA AGTAATCATT TTGCTCTGTG CCCTGCTGGC CTTGACCATG
      781 GCCGCAGTTC CCCTGCTGGG TGGCAGCAAG TATGGCGCCT CCCCTCTCTG CCTGCCTTTG
      841 CCTTTTGGGG AGCCCAGCAC CATGGGCTAC ATGGTCGCTC TCATCTTGCT CAATTCCCTT
      901 TGCTTCCTCA TGATGACCAT TGCCTACACC AAGCTCTACT GCAATTTGGA CAAGGGAGAC
      961 CTGGAGAATA TTTGGGACTG CTCTATGGTA AAACACATTG CCCTGTTGCT CTTCACCAAC
     1021 TGCATCCTAA ACTGCCCTGT GGCTTTCTTG TCCTTCTCCT CTTTAATAAA CCTTACATTT
     1081 ATCAGTCCTG AAGTAATTAA GTTTATCCTT CTGGTGGTAG TCCCACTTCC TGCATGTCTC
     1141 AATCCCCTTC TCTACATCTT GTTCAATCCT CACTTTAAGG AGGATCTGGT GAGCCTGAGA
     1201 AAGCAAACCT ACGTCTGGAC AAGATCAAAA CACCCAAGCT TGATGTCAAT TAACTCTGAT
     1261 GATGTCGAAA AACAGTCCTG TGACTCAACT CAAGCCTTGG TAACCTTTAC CAGCTCCAGC
     1321 ATCACTTATG ACCTGCCTCC CAGTTCCGTG CCATCACCAG CTTATCCAGT GACTGAGAGC
     1381 TGCCATCTTT CCTCTGTGGC ATTTGTCCCA TGTCTCTAAG AATTCGAGCT CGGTACCTCG
     1441 CGAATGCATC AGATCTGATC CTCGAGGGCC GGCGCGCCGC GGCCGCTACG TAAATTCCGC
     1501 CCCTCTCCCT AACGTTACTG GCCGAAGCCG CTTGGAATAA GGCCGGTGTG CGTTTGTCTA
     1561 TATGTTATTT TCCACCATAT TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AACCTGGCCC
     1621 TGTCTTCTTG ACGAGCATTC CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT
     1681 GTTGAATGTC GTGAAGGAAG CAGTTCCTCT GGAAGCTTCT TGAAGACAAA CAACGTCTGT
     1741 AGCGACCCTT TGCAGGCAGC GGAACCCCCC ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA
     1801 GCCACGTGTA TAAGATACAC CTGCAAAGGC GGCACAACCC CAGTGCCACG TTGTGAGTTG
     1861 GATAGTTGTG GAAAGAGTCA AATGGCTCTC CTCAAGCGTA TTCAACAAGG GGCTGAAGGA
     1921 TGCCCAGAAG GTACCCCATT GTATGGGATC TGATCTGGGG CCTCGGTGCA CATGCTTTAC
     1981 ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG ACGTGGTTTT
     2041 CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCGCCAC CATGGTGAGC AAGGGCGAGG
     2101 AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA
     2161 AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT
     2221 TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT
     2281 ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT
     2341 CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT
     2401 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA
     2461 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA
     2521 ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA
     2581 AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA
     2641 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG
     2701 CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG
     2761 CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTA AGCGGCCGCC ACCGCGGGGA
     2821 GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA
     2881 AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC
     2941 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG
     3001 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT
     3061 CCCGGCTGCC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTTGACACA TGCAGCTCCC
     3121 GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC
     3181 GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGAGG TCGACTCTAG TCCCCGCGGT
     3241 GGCAGATCTG ATCTTTCCac tcAAAACatA TAACTTCGTA TAgcatacat TATACGAAGT
     3301 TATatTCAAA AATCTAGAca GCGGCCGCCa CTCAAAATTT CTTCTATAAA GTAACAAAAC
     3361 TTTTATGAGG GACAGCCCCC CCCCAAAGCC CCCAGGGATG TAATTACGTC CCTCCCCCGC
     3421 TAGGGGGCAG CAGCGAGCCG CCCGGGGCTC CGCTCCGGTC CGGCGCTCCC CCCGCATCCC
     3481 CGAGCCGGCA GCGTGCGGGG ACAGCCCGGG CACGGGGAAG GTGGCACGGG ATCGCTTTCC
     3541 TCTGAACGCT TCTCGCTGCT CTTTGAGCCT GCAGACACCT GGGGGGATAC GGGGAAAAGG
     3601 CCTCCAAGGC CttaaTTCGg ccgcaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     3661 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     3721 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     3781 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     3841 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     3901 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     3961 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     4021 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     4081 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     4141 ttacagatcc aagctgtgac cggcgcctac gatatcgcca ccatggccag ctccgaggat
     4201 gtcatcaaag agtttatgag atttaaggtc aagatggagg gaagcgtcaa cggacacgag
     4261 ttcgagattg agggagaagg agaaggccgg ccttacgagg gcacacaaac cgctaagctc
     4321 aaggtcacaa aaggaggacc cctccccttc tcctgggata ttctgagccc tcagttccag
     4381 tacggaagca aagcctatgt taaacaccct gccgacatcc ctgactatct gaagctctcc
     4441 ttccctgaag gcttcaagtg ggagagattc atgaacttcg aggacggagg cgtggtgaca
     4501 gtcacacaag atagcaccct ccaggacgga gagtttattt ataaggtgaa actcagagga
     4561 accaacttcc cctccgatgg ccctgtcatg caaaaaaaaa caatgggatg ggaagcctcc
     4621 accgagagaa tgtatcctga ggatggcgct ctgaaaggcg aaattaaaat gagactgaaa
     4681 ctcaaagacg gaggacacta cgatgccgag gtcaaaacaa cctacaaggc caagaaacaa
     4741 gtgcagctgc ctggcgccta catgactgat attaaactcg acattatcag ccataatggg
     4801 gactacacca tcgtggaaca atatgagaga gctgagggca gacatagcac aggcgctgga
     4861 GAGGGCAGAG GAAGTCTTCT AACATGCGGT GACGTGGAGG AGAATCCCGG CCCTatgacc
     4921 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     4981 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     5041 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5101 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     5161 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     5221 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     5281 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     5341 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     5401 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     5461 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     5521 cctctggaCG AAAttCCGCG Gttattacaa aatttgtgaa agattgactg gtattcttaa
     5581 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcaGTTAAC
     5641 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT
     5701 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT
     5761 CATGTCTGGA ATTGACTCAA ATGATGTCAA TTAGTCTATC AGAAGCTATC TGGTCTCCCT
     5821 TCCGGGGGAC AAGACATCCC TGTTTAATAT TTAAACAGCA GTGTTCCCAA ACTGGGTTCT
     5881 TATATCCCTT GCTCTGGTCA ACCAGGTTGC AGGGTTTCCT GTCCTCACAG GAACGAAGTC
     5941 CCTAAAGAAA CAGTGGCAGC CAGGTTTAGC CCCGGAATTG ACTGGATTCC TTTTTTAGGG
     6001 CCCATTGGTA TGGCTTTTTC CCCGTATCCC CCCAGGTGTC TGCAGGCTCA AAGAGCAGCG
     6061 AGAAGCGTTC AGAGGAAAGC GATCCCGTGC CACCTTCCCC GTGCCCGGGC TGTCCCCGCA
     6121 CGCTGCCGGC TCGGGGATGC GGGGGGAGCG CCGGACCGGA GCGGAGCCCC GGGCGGCTCG
     6181 CTGCTGCCCC CTAGCGGGGG AGGGACGTAA TTACATCCCT GGGGGCTTTG GGGGGGGGCT
     6241 GTCCCTGATA TCAAACatAT AACTTCGTAT AgcatacatT ATACGAAGTT ATtTTATCAC
     6301 GTAAGTAGAA CATGAAAtaa cCTAGATCGG ATCCAAGGCT GAAAACCTCT TGATACTTGA
     6361 GAGTGAATAT AAGTCTAAAT GCTGCTTTGT ATAATTTGTT CAGCTAAGGG ATAGATCGAT
     6421 CACACTATTT AAGTGAGCCC AGATCAAAAA AGCAGATTGA AATTTTCTTT AGAAAAGATT
     6481 CTCCATGATT TGAATTGCAT TCTCTTTAAA CTCACCAATG TAATCATTTT GGGAGGAGGG
     6541 AGAACCCACT TGCTTTCCAA ATGGGTTTAT TTAAACCCAC AAACTCAAGA GGTTGTTGGG
     6601 GGAATTAGGA AAATAAGGGT TTTCAATGAC CTACATTGCT AGGTAGAGGC TGTGATCCAT
     6661 GGGATTTCAT TCTAATGACC ATGTGAAGAT GTTTGAGTCC TCCTTTGCCT TTCCTCAGAA
     6721 AGAATCCTTC TAAGGCACAA ATCCCTTAGA TGGATAATGT AAGGTATTGT TAACTCACTC
     6781 ATATTGAGAT CATTTTTAGA GATACCAGGT TTTATGTATC AGCACTAGAT GGTTCCACCC
     6841 TCATGGGATA AAACTGCTTA CAAGTATTTT GAAAGAAAAA CTGACCAAAA TTCTTAAATT
     6901 GTTACTAAGG CAATCATGCA CAGGTGACGT ATGTCTTATC TGATTTGTTT TTAACTCCTT
     6961 GGTGCCCAAA GCTCAGAAGG GAATTCCACT GCCAGCAATG AACATACCTG GAAAAGAAAG
     7021 TAAGCAATCT GGGATTTTTT TTCTGGGTTA GTAAAGAATT TTTGCAATAA GTTTTATCAG
     7081 TTGATTCAAA CTGATGTGCA TCTTAATGAT CAAATGTGCA CATTACATAA ATTAAGTCCA
     7141 CTGATACAAC TTCTTACACA TGTATCTCTA GTAGCTCTGG CAAACCCAAT ATCTGACACC
     7201 ACTTTGGACT CAAGAGACTC AGTAACGTAT TATCCTGTTT ATTTAGCTTG GTTTTAGCTG
     7261 TGTTCTCTCT GGATAACCCA CTTGATGTTA GGAACATTAC TTCTCTGCTT ATTCCATATT
     7321 AATACTGTGT TAGGTATTTT AAGAAGCAAG TTATTAAATA AGAAAAGTCA AAGTATTAAT
     7381 TCTTACCTTC TATTATCCTA TATTAGCTTC AATACATCCA AACCAAATGG CTGTTAGGTA
     7441 GGATCCCCGT CGACTGCATG CAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG
     7501 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC
     7561 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT
     7621 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
     7681 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
     7741 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC
     7801 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
     7861 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     7921 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     7981 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     8041 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     8101 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     8161 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     8221 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     8281 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     8341 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     8401 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     8461 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     8521 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     8581 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     8641 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     8701 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     8761 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     8821 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     8881 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     8941 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     9001 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     9061 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     9121 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     9181 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     9241 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     9301 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     9361 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     9421 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     9481 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     9541 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     9601 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC
     9661 ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT C
//
