LOCUS       Exported                4732 bp ds-DNA     circular SYN 20-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 21, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    53..760
                     /label=mNeonGreen
                     /note="note=""direct strand translation"" 
                     translation=""MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTEL"
     terminator      784..971
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            1046..2246
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5 
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        1046..1389
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1390..2043
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHTNQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMSNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      2049..2246
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2351..2369)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2627..3215)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3386..4246)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4247..4351)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4697..4715
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 agcttcgtac gctgcaggtc gacggatcgg tgacggtgct ggtttaatta acatggtgag
       61 taaaggcgag gaggacaata tggcgagctt acctgctacc cacgaactgc acattttcgg
      121 ctcaatcaat ggtgtagatt ttgacatggt cggtcagggg actggtaacc ctaacgatgg
      181 ttatgaggag ctaaatctga aatctactaa aggggacctt caatttagtc cctggatact
      241 tgttccgcac ataggttatg gtttccacca atatttgccc taccctgacg ggatgagccc
      301 cttccaggcg gctatggtag atggcagtgg ctatcaggtc cacagaacaa tgcaatttga
      361 ggacggggcc agcctaaccg tgaattatag gtacacgtac gaggggtcac atataaaggg
      421 cgaagcacag gtgaagggga cggggttccc ggcggacgga ccagtgatga caaactccct
      481 gaccgctgcc gattggtgcc gtagtaagaa gacttatcct aacgacaaaa cgataatcag
      541 taccttcaag tggagttata ccacgggcaa cgggaaaaga taccgtagca cggcgagaac
      601 aacttatact ttcgccaagc caatggctgc caactacttg aagaatcagc ccatgtatgt
      661 attccgtaag acggagctga aacatagcaa aaccgaacta aacttcaagg agtggcagaa
      721 agcgtttacc gatgtgatgg gtatggacga attgtataag taaggcgcgc cacttctaaa
      781 taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt
      841 gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac
      901 tctttcctgt aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac
      961 acctctaccg gcagatccgc tagggataac agggtaatat agatctgttt agcttgcctc
     1021 gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct
     1081 tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc
     1141 ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta
     1201 cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg
     1261 tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt
     1321 ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca
     1381 taaacaacca tgggtaggag ggcttttgta gaaagaaata cgaacgaaac gaaaatcagc
     1441 gttgccatcg ctttggacaa agctccctta cctgaagagt cgaattttat tgatgaactt
     1501 ataacttcca agcatacaaa ccaaaaggga gaacaagtaa tccaagtaga cacgggaatt
     1561 ggattcttgg atcacatgta tcatgcactg gctaaacatg caggctggag cttacgactt
     1621 tactcaagag gtgatttaat catcgatgat catcacactg cagaagatac tgctattgca
     1681 cttggtattg cattcaagca ggctatgagt aactttgccg gcgttaaaag atttggacat
     1741 gcttattgtc cacttgacga agctctttct agaagcgtag ttgacttgtc gggacggccc
     1801 tatgctgtta tcgatttggg attaaagcgt gaaaaggttg gggaattgtc ctgtgaaatg
     1861 atccctcact tactatattc cttttcggta gcagctggaa ttactttgca tgttacctgc
     1921 ttatatggta gtaatgacca tcatcgtgct gaaagcgctt ttaaatctct ggctgttgcc
     1981 atgcgcgcgg ctactagtct tactggaagt tctgaagtcc caagcacgaa gggagtgttg
     2041 taaagagtac tgacaataaa aagattcttg ttttcaagaa cttgtcattt gtatagtttt
     2101 tttatattgt agttgttcta ttttaatcaa atgttagcgt gatttatatt ttttttcgcc
     2161 tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc
     2221 gtatgtgaat gctggtcgct atactgctgt cgattcgata ctaacgccgc catccagttt
     2281 aaacgagctc gaattcatcg atgatatcag atccactagt ggcctatgcg gccgcggatc
     2341 tgccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac ctgcattaat
     2401 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2461 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2521 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2581 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2641 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2701 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2761 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2821 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2881 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2941 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3001 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3061 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3121 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3181 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3241 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3301 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3361 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3421 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3481 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3541 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3601 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3661 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3721 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3781 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3841 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3901 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3961 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4021 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4081 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4141 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4201 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4261 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4321 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4381 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4441 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4501 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4561 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     4621 gagtgcacca tatggacata ttgtcgttag aacgcggcta caattaatac ataaccttat
     4681 gtatcataca catacgattt aggtgacact atagaacgcg gccgccagct ga
//
