LOCUS       Exported                5598 bp ds-DNA     circular SYN 28-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pZEG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Jun 23, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(430..440)
                     /label=pZEU/pZCA-URA3-NAT-F
     primer_bind     590..614
                     /label=pZEU-2F
     primer_bind     667..725
                     /label=pZEU/pZCA-URA3-NAT-F
     primer_bind     737..770
                     /label=loxP
     promoter        771..1168
                     /label=pAgTEF
                     /note=" kanMX TEF promoter sequence from the filamentous 
                     fungus Ashbya gossypii (Wash et al., 1994; Guldener et al.,
                     1996)."
     gene            1169..1978
                     /label=kanMX
                     /note="kanMX"
     primer_bind     complement(1300..1315)
                     /label=kanMX-R1
     primer_bind     1952..1968
                     /label=kanMX-F1
     terminator      1979..2243
                     /label=tAgTEF
                     /note=" kanMX TEF terminator sequence from the filamentous 
                     fungus Ashbya gossypii (Wash et al., 1994; Guldener et al.,
                     1996)."
     CDS             2244..2277
                     /codon_start=1
                     /label=loxP
                     /translation="ITSYNVCYTKL"
     primer_bind     complement(2288..2354)
                     /label=pZEU/pZCA-URA3-kanMX-F
     primer_bind     complement(2555..2577)
                     /label=pZEU-2R
     misc_feature    2659..3652
                     /label=pSR1 replicon
                     /note="pSR1 replicon"
     rep_origin      complement(3779..4367)
                     /direction=LEFT
                     /label=ori
     CDS             complement(4538..5398)
                     /codon_start=1
                     /label=AmpR
                     /note="gene=""bla""
                     function=""ampicillin resistance"" 
                     product=""beta lactamase"""
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5399..5503)
                     /label=pAmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      601 gctttccagt cgggaaacct gtcgtgccag cagatctgag ctttttcttt ccaatttttt
      661 ttttttgtga gtttagtata catgcattta cttataatac agttttttcg tacgctgcag
      721 gtcgacaacc cttaatataa cttcgtataa tgtatgctat acgaagttat taggtctaga
      781 gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga
      841 ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt
      901 acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca
      961 cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc
     1021 tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg
     1081 atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt
     1141 ctcacatcac atccgaacat aaacaaccat gggtaaggaa aagactcacg tttcgaggcc
     1201 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     1261 cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt
     1321 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     1381 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     1441 tgcatggtta ctcaccactg cgatccccgg caaaacagca ttccaggtat tagaagaata
     1501 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     1561 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     1621 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     1681 gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt
     1741 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     1801 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     1861 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     1921 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     1981 agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat
     2041 attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac
     2101 atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg
     2161 tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa
     2221 acgagctctc gagaaccctt aatataactt cgtataatgt atgctatacg aagttattag
     2281 gtgatatcag atccactagt ggcctatgcg atttatcttc gtttccggtt tttgttctgt
     2341 gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat
     2401 ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat
     2461 caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga
     2521 aaagccagat ctgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2581 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2641 agcggtatca gcatcgatag atcctacaaa atatgtaatg ctattcttag attcccacga
     2701 gaggcttgct tttgtacata gaccctccag aaaacatagt gctgtttatg cagcacacag
     2761 aaggtttata ggtgtgagct acactttata ggccgggtaa gtatagtaag atatgtgcaa
     2821 attagccgcg gagccataaa caatcatctt ttaaattaga tgcacagcct taaaccaagc
     2881 aattctggtc acctctccgg attttgggtg aatatcaaaa tgacctcaca aggtaacact
     2941 ttggatgata ttcggttaca agtcaggtgt gacattctag gtaaagacac caacagaaga
     3001 ggaggctctg agaaggatcc tgaggatcaa agtgaaactg agtccagtgg atagactcat
     3061 ttgctaattt aagcctttct gtaagactat tgagaccgtg tactattaat ttttcttatt
     3121 gtaacagtag gtaataggta agggtataaa aatcatgggt ataatgataa atttgtattg
     3181 caataatgtc caaatcaaat tcggaggcga ggtccaagaa gcaagatggt gtatggtagc
     3241 aatatagaga gggttttatt gggttattat tttattttca agaatttaca ttttgagtgt
     3301 agatctttac tttcagcaag aaggtacgag gaacaagcgg gtagtttaga ggaacaaaat
     3361 aaaaatacaa gagcatagta actgtcaggg tgtatcaact ggaccaatca ttgcttctcg
     3421 tagtcaaatg tttattcgat agagtaatgg tttaatttct gtagcaaccg cctgcgcagg
     3481 ctcccttctg cgcggtacgg gttgatgaaa gaacgacttg atgaaagaac gatttgatga
     3541 aagaacgaat taatgaaaga acgatttgat gaaagaataa cgtattcttt catcaacgtc
     3601 cacagtgata tgatctcgcc tagaaaaact aaattatatt aagctaaact aaatcgatgc
     3661 tcactcaaag gtcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3721 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3781 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3841 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3901 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3961 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4021 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4081 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4141 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4201 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4261 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4321 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4381 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4441 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4501 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     4561 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     4621 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     4681 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4741 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4801 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4861 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4921 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     4981 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5041 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5101 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5161 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5221 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5281 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5341 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5401 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5461 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     5521 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     5581 tcacgaggcc ctttcgtc
//
