LOCUS       Exported                5219 bp ds-DNA     circular SYN 23-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pZEN
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Jun 23, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     195..240
                     /label=CRE-pZEU-SspDI-F
                     /note="Codina di CRE-pZEU-SspDI-F su pZEU"
     primer_bind     357..400
                     /label=pZEU-MATalpha2 cp1-F
     primer_bind     complement(397..441)
                     /label=pZEU-MATalpha1 cp1-R
     primer_bind     complement(452..491)
                     /label=CRE-pZEU-HindIII-R
                     /note="Codina di CRE-pZEU-HindIII-R  su pZEU"
     misc_feature    508..591
                     /label=lacZ
     primer_bind     590..614
                     /label=pZEU-2F
     primer_bind     664..703
                     /label=pZEU/pZCA-URA3-NAT-F
     CDS             join(704..713,1368..1405)
                     /codon_start=1
                     /label=C.glabrata ACT1
                     /translation="MDGEEVAALVIDNGSH"
     primer_bind     952..971
                     /label=SAT1gene RC
     CDS             1406..1930
                     /codon_start=1
                     /label=SAT1 ORF
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSPY
                     RKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSL
                     IEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMY
                     WYWFSGAQDDA"
     primer_bind     1416..1436
                     /label=SAT1 - Fb
     primer_bind     1863..1880
                     /label=SAT1-1
     primer_bind     complement(1908..1930)
                     /label=pZEU-URA3-NAT-R
     misc_feature    1931..2142
                     /label=pScURA3
     primer_bind     complement(2176..2198)
                     /label=pZEU-2R
     misc_feature    2280..3273
                     /label=pSR1 replicon
                     /note="pSR1 replicon"
     rep_origin      3400..3988
                     /label=ori
     CDS             complement(4159..5019)
                     /codon_start=1
                     /label=AmpR
                     /note="gene=""bla"" 
                     function=""ampicillin resistance"" 
                     product=""beta lactamase"""
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5020..5124)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      601 gctttccagt cgggaaacct gtcgtgccag cagatctggc tttttctttc caattttttt
      661 tttgtgagtt tagtatacat gcatttactt ataatacagt tttatggacg gtggtatgtt
      721 ttagtttagc ttcaattcta attgattgat taatcagttg attggtttca atatgacaaa
      781 tgggtagggt gggaaaactt cattttcaat tcagatcaaa cttttttgtt gtcgacataa
      841 tatttctcgt ttgggatgtt actgtcacat taataataca cacacatcag cttataattt
      901 tgaaagtaat ttatcagata tgttgtgacg atcaatggaa atggctaact tcaatgtatc
      961 tgttcttccc ctttttcaaa gttcacgttt tttgattgat tgattgatct gtcggcagtg
     1021 gtttcaaaac cattcggtga gtaatcctat caatcaatgt tacgacaaaa ggctcaatat
     1081 tcaaaattgc aatgttttat gttttcctac gtgtacttgt gcaaggcaat tgattcaaca
     1141 ttgcttttgg tgtttgacga gtttctagtt tggacttgtg ttgttatctg ggctatacag
     1201 atttcccggc tcactatgaa tttttttttt cgacgctcag tgcacacaac tataaacaac
     1261 acaaacacaa acacagcaag aaaaaaaaaa aacgaacatt gaattgaaac caagccaact
     1321 gaaaaattcc ttatttaaat gactgtcata ctaacccatt tttatagaag aagttgctgc
     1381 tttagttatc gataacggtt ctcatatgaa aatttcggtg atccctgagc aggtggcgga
     1441 aacattggat gctgagaacc atttcattgt tcgtgaagtg ttcgatgtgc acctatccga
     1501 ccaaggcttt gaactatcta ccagaagtgt gagcccctac cggaaggatt acatctcgga
     1561 tgatgactct gatgaagact ctgcttgcta tggcgcattc atcgaccaag agcttgtcgg
     1621 gaagattgaa ctcaactcaa catggaacga tctagcctct atcgaacaca ttgttgtgtc
     1681 gcacacgcac cgaggcaaag gagtcgcgca cagtctcatc gaatttgcga aaaagtgggc
     1741 actaagcaga cagctccttg gcatacgatt agagacacaa acgaacaatg tacctgcctg
     1801 caatttgtac gcaaaatgtg gctttactct cggcggcatt gacctcttca cgtataaaac
     1861 tagacctcaa gtctcgaacg aaacagcgat gtactggtac tggttctcgg gagcacagga
     1921 tgacgcctaa gatttatctt cgtttccggt ttttgttctg tgcagttggg ttaagaatac
     1981 tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc taagtctgtg
     2041 ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaagaaa
     2101 ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aaaagccaga tctgctgcat
     2161 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2221 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agcatcgata
     2281 gatcctacaa aatatgtaat gctattctta gattcccacg agaggcttgc ttttgtacat
     2341 agaccctcca gaaaacatag tgctgtttat gcagcacaca gaaggtttat aggtgtgagc
     2401 tacactttat aggccgggta agtatagtaa gatatgtgca aattagccgc ggagccataa
     2461 acaatcatct tttaaattag atgcacagcc ttaaaccaag caattctggt cacctctccg
     2521 gattttgggt gaatatcaaa atgacctcac aaggtaacac tttggatgat attcggttac
     2581 aagtcaggtg tgacattcta ggtaaagaca ccaacagaag aggaggctct gagaaggatc
     2641 ctgaggatca aagtgaaact gagtccagtg gatagactca tttgctaatt taagcctttc
     2701 tgtaagacta ttgagaccgt gtactattaa tttttcttat tgtaacagta ggtaataggt
     2761 aagggtataa aaatcatggg tataatgata aatttgtatt gcaataatgt ccaaatcaaa
     2821 ttcggaggcg aggtccaaga agcaagatgg tgtatggtag caatatagag agggttttat
     2881 tgggttatta ttttattttc aagaatttac attttgagtg tagatcttta ctttcagcaa
     2941 gaaggtacga ggaacaagcg ggtagtttag aggaacaaaa taaaaataca agagcatagt
     3001 aactgtcagg gtgtatcaac tggaccaatc attgcttctc gtagtcaaat gtttattcga
     3061 tagagtaatg gtttaatttc tgtagcaacc gcctgcgcag gctcccttct gcgcggtacg
     3121 ggttgatgaa agaacgactt gatgaaagaa cgatttgatg aaagaacgaa ttaatgaaag
     3181 aacgatttga tgaaagaata acgtattctt tcatcaacgt ccacagtgat atgatctcgc
     3241 ctagaaaaac taaattatat taagctaaac taaatcgatg ctcactcaaa ggtcggtaat
     3301 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3361 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3421 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3481 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3541 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3601 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3661 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3721 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3781 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3841 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3901 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3961 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4021 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4081 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4141 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4201 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4261 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4321 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4381 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4441 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4501 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4561 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4621 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4681 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4741 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4801 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4861 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4921 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4981 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5041 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5101 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     5161 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
