LOCUS       Exported                7779 bp ds-DNA     circular SYN 23-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGRCRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   stcass
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Jun 23, 2019 from SnapGene Viewer 4.3.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     3..21
                     /label=CEN-glabrata-F
     misc_feature    66..664
                     /label=CEN\ARS C. glabrata
     misc_feature    670..737
                     /label=Amp-R promoter
                     /note="partial sequence"
     CDS             738..1598
                     /codon_start=1
                     /label=Amp-R
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1693..2375
                     /label=pBM1(ColE1) origin
     primer_bind     2466..2486
                     /label=pGRB-F4
     primer_bind     2568..2588
                     /label=pGRB-F3
     primer_bind     2774..2838
                     /label=CRE-pGRB-SacI-XbaI-F1
     misc_feature    2817..2828
                     /label=sequenza_aggiunta_da pZCRE
     misc_feature    2829..3295
                     /label=GAL1 promoter
     primer_bind     complement(2995..3017)
                     /label=GAL-F1
     CDS             3404..4435
                     /codon_start=1
                     /label=Cre
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRTLDSETGAMVRLLE
                     DGD"
     primer_bind     3460..3479
                     /label=RT-CRE-F2n
     primer_bind     complement(4026..4046)
                     /label=RT-CRE-R2
     primer_bind     4120..4140
                     /label=CRE-R1
     misc_feature    4438^4439
                     /label=deleted 40 bp
     primer_bind     complement(4601..4623)
                     /label=pGRB-R3
     primer_bind     complement(4821..4842)
                     /label=pGRB-R4
     CDS             join(6013..6022,6677..6714)
                     /codon_start=1
                     /transl_table=11
                     /label=C. glabrata ACT1
                     /translation="MDGEEVAALVIDNGSH"
     primer_bind     complement(6153..6174)
                     /label=SAT1-2R
     CDS             6715..7239
                     /codon_start=1
                     /label=SAT1
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSPY
                     RKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSL
                     IEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMY
                     WYWFSGAQDDA"
     primer_bind     6725..6745
                     /label=SAT1-Fb
     primer_bind     7122..7145
                     /label=SAT1-R1
     primer_bind     7172..7189
                     /label=SAT1-1
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagcaggt tagactaaca tgcaaaaagt ttatattcat ttttgtagtt attcttgttt
      121 tcattaggat gattacacac tgtggatgtg gattaatcga tcattgttaa tttagtgtag
      181 ctactaccat ttcaaacaaa acgtaaattc ttgcagtcgt actggatctg tgaatctatt
      241 agtatatatg aattaaagta gcttgtacat attattctgt tgaatcatat cgcagagcat
      301 tggttgaaat ccaaaatata aaaatgtaat atcacaaaaa ataatactaa ttctaacatt
      361 aatgggtcag atttttagtg aatacttaaa tttataatat cgtctattta agctagcaaa
      421 tggaacaaca tttaaagtaa gaacatcata tctacatgaa aatgtatatt tcaatctgac
      481 taataacgca gagcacatct ttcagtgatg tctgtcacat gatcaaaaag aattgtattt
      541 aaataattca taataaaagc ttaaaaaatt acaataatga aaataaagta aataatgaca
      601 tgggtaagag ttccgaaata gaatcttagt gtacaaaaca aaaattgcat cattagagat
      661 ccccacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      721 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      781 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      841 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      901 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      961 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1021 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1081 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1141 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1201 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1261 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1321 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1381 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1441 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     1501 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     1561 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     1621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     1921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2521 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2581 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     2641 caattaatgt gagttacctc actcattagg caccccaggc tttacacttt atgcttccgg
     2701 ctcctatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     2761 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctcgaat
     2821 tcgagctcta gtacggatta gaagccgccg agcgggtgac agccctccga aggaagactc
     2881 tcctccgtgc gtcctcgtct tcaccggtcg cgttcctgaa acgcagatgt gcctcgcgcc
     2941 gcactgctcc gaacaataaa gattctacaa tactagcttt tatggttatg aagaggaaaa
     3001 attggcagta acctggcccc acaaaccttc aaatgaacga atcaaattaa caaccatagg
     3061 atgataatgc gattagtttt ttagccttat ttctggggta attaatcagc gaagcgatga
     3121 tttttgatct attaacagat atataaatgc aaaaactgca taaccacttt aactaatact
     3181 ttcaacattt tcggtttgta ttacttctta ttcaaatgta ataaaagtat caacaaaaaa
     3241 ttgttaatat acctctatac tttaacgtca aggagaaaaa accccggatt ctagaactag
     3301 tggatccccc gggctgcagg aattcgatat caagcttatc gataccgtcg aggggcagag
     3361 ccgatcctgt acactttact taaaaccatt atctgagtgt taaatgtcca atttactgac
     3421 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa
     3481 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct
     3541 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc
     3601 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa
     3661 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg ggctgccacg
     3721 accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga
     3781 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg
     3841 ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat ttctggggat
     3901 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc
     3961 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac
     4021 cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc
     4081 cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg
     4141 tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga
     4201 agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg
     4261 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat
     4321 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg
     4381 taccctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg attagccata
     4441 tatatataac gtacacatag aaatcacaca aacagagtat ttattcttaa ctacatgaac
     4501 taccatcaga ccgtctgggc cactatataa tgtgccattc ataaacgtga tcactttacg
     4561 tagcaggcaa ccccaggtga aaatttttca gcgagctgcc agattgtcag gtgaaaaact
     4621 gaaaaaaact tctgggcgat gagcttgtgg cgggaaatta agtatataaa gcagttagtt
     4681 tcttctgctt cttgtggttc tgggtattct tgtaatattt ggaagaatag aaaatcaaga
     4741 ctactgcctt tcttttcata ttatagagga agaaatacgc acgaacacga tatagaggta
     4801 aaggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     4861 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4921 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4981 cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5041 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5101 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5161 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5221 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5281 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5341 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5401 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     5461 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagggtaat
     5521 aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat
     5581 aatacagtat aaatcattgt gctatttata ttatatagtt tactcataat accataaaat
     5641 attcggtttg attaggttat tttaataagc taatttgttt ctaatcgtgt aatttatgct
     5701 gtgtatatta agtagtgtgt gcactgccca aaaatgtttg ttgtttatag tcggttaaag
     5761 agaaaaaaga aaaaaagatc catacacaca cgttaattag ttgttcaacg taatacactc
     5821 atattttgtt cttatttgct ttcggtcgct gttctcacca agatttattg ccaacgaaac
     5881 aatttttttt tatatatttt cagatttttc tttttttcct ttcctttcct tttctaattt
     5941 tcactcctgg ttttctttct ttcttagaaa cattatctcg atattaatat taaaaaaata
     6001 taatcattca aaatggacgg tggtatgttt tagtttagct tcaattctaa ttgattgatt
     6061 aatcagttga ttggtttcaa tatgacaaat gggtagggtg ggaaaacttc attttcaatt
     6121 cagatcaaac ttttttgttg tcgacataat atttctcgtt tgggatgtta ctgtcacatt
     6181 aataatacac acacatcagc ttataatttt gaaagtaatt tatcagatat gttgtgacga
     6241 tcaatggaaa tggctaactt caatgtatct gttcttcccc tttttcaaag ttcacgtttt
     6301 ttgattgatt gattgatctg tcggcagtgg tttcaaaacc attcggtgag taatcctatc
     6361 aatcaatgtt acgacaaaag gctcaatatt caaaattgca atgttttatg ttttcctacg
     6421 tgtacttgtg caaggcaatt gattcaacat tgcttttggt gtttgacgag tttctagttt
     6481 ggacttgtgt tgttatctgg gctatacaga tttcccggct cactatgaat tttttttttc
     6541 gacgctcagt gcacacaact ataaacaaca caaacacaaa cacagcaaga aaaaaaaaaa
     6601 acgaacattg aattgaaacc aagccaactg aaaaattcct tatttaaatg actgtcatac
     6661 taacccattt ttatagaaga agttgctgct ttagttatcg ataacggttc tcatatgaaa
     6721 atttcggtga tccctgagca ggtggcggaa acattggatg ctgagaacca tttcattgtt
     6781 cgtgaagtgt tcgatgtgca cctatccgac caaggctttg aactatctac cagaagtgtg
     6841 agcccctacc ggaaggatta catctcggat gatgactctg atgaagactc tgcttgctat
     6901 ggcgcattca tcgaccaaga gcttgtcggg aagattgaac tcaactcaac atggaacgat
     6961 ctagcctcta tcgaacacat tgttgtgtcg cacacgcacc gaggcaaagg agtcgcgcac
     7021 agtctcatcg aatttgcgaa aaagtgggca ctaagcagac agctccttgg catacgatta
     7081 gagacacaaa cgaacaatgt acctgcctgc aatttgtacg caaaatgtgg ctttactctc
     7141 ggcggcattg acctcttcac gtataaaact agacctcaag tctcgaacga aacagcgatg
     7201 tactggtact ggttctcggg agcacaggat gacgcctaac atatgtgaag tgtgaagggg
     7261 gagattttca ctttattaga tttgtatata tgtataataa ataaataaat aagttaaata
     7321 aataattaga taagggtggt aattattact atttacaatc aaaggtggtc ctgcaggctt
     7381 cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt
     7441 tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct
     7501 tccttctgtt cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa
     7561 gaataaaaaa aaaatgatga attgaaaagg tggtatggtg cactctcagt acaatctgct
     7621 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7681 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7741 tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
