LOCUS Exported 6955 bp ds-DNA circular SYN 24-JUN-2019 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6955) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, Jun 24, 2019 from SnapGene Viewer 4.3.10 https://www.snapgene.com COMMENT Ligation of : pAAV-CAG-FLEX-YMAT-PDGFR-3.xdna [5064 nt] : (SpeI[2952] / XhoI[1113]) to PCR(pMinDis-L310E10-PDGFR-EnhExp.xdna) [1883 nt] : (XhoI[1891] / SpeI[4]) FEATURES Location/Qualifiers source 1..6955 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..249 /label=CMV Enhancer /note="color: #84a4c0; direction: RIGHT" misc_feature 251..528 /label=Chicken B-actin Promoter /note="color: #92abc4; direction: RIGHT" primer_bind 808..830 /label=CAG-F /note="color: #9b43df; direction: RIGHT" misc_feature 892..911 /label=pCAG_F_primer /note="label=""pCAG_F_primer"" - ApEinfo_revcolor=#f58a5e - ApEinfo_fwdcolor=#f58a5e - 3542..3561" /note="color: #9b43df; direction: RIGHT" misc_feature 946..979 /label=lox2722 /note="label=""loxP"" - ApEinfo_revcolor=#9eafd2 - ApEinfo_fwdcolor=#9eafd2 - 3596..3629" /note="color: #1da0ff; direction: RIGHT" misc_feature 1042..1075 /label=LoxP /note="color: #0000ff; direction: RIGHT" misc_feature complement(1122..1199) /label=ProposedExportMotifs_perLL_123016_misc_feature /note="note=""ProposedExportMotifs_perLL_123016"" - 975..1052" /note="color: #0378ff; direction: LEFT" misc_feature complement(1191..1259) /label=PDGFR transmembrane helix_misc_feature /note="note=""PDGFR transmembrane helix"" - 915..983" /note="color: #529cff; direction: LEFT" CDS complement(1326..1355) /label=c-Myc tag /note="color: #008000; direction: LEFT" CDS complement(1326..1355) /label=Myc_Myc (human c-Myc oncogene) epitope tag_CDS /note="codon_start=1 - note=""Myc (human c-Myc oncogene) epitope tag"" - note=""Myc"" - translation=""EQKLISEEDL"" - 819..848" /note="color: #f08080; direction: LEFT" misc_feature complement(1362..1955) /label=X513OpuB 107-305 /note="note=""X513OpuB 107-305"" - 1774..2367" /note="color: #dc143c; direction: LEFT" misc_feature complement(1956..1964) /label=Linker (C) /note="color: #0378ff; direction: LEFT" misc_feature complement(1965..2687) /label=cpsfGFP /note="color: #5fff45; direction: LEFT" misc_feature complement(2688..2699) /label=Linker (N) /note="color: #0378ff; direction: LEFT" misc_feature complement(2700..2924) /label=X513OpuB 30-106 /note="note=""X513OpuB 30-106"" - 805..1029" /note="color: #dc143c; direction: LEFT" misc_feature complement(2925..2930) /label=MCS_misc_feature /note="note=""MCS"" - 799..804" /note="color: #b5978d; direction: LEFT" CDS complement(2931..2993) /label=Ig-kappa leader_leader sequence from mouse_CDS /note="codon_start=1 - note=""leader sequence from mouse"" - note=""Ig-kappa leader"" - translation=""METDTLLLWVLLLWVPGSTGD"" - 737..799" /note="color: #f08080; direction: LEFT" misc_feature 3028..3061 /label=lox2722 /note="color: #1792ff; direction: RIGHT" misc_recomb 3132..3165 /label=LoxP /note="color: #0000ff; direction: RIGHT" misc_feature 3209..3796 /label=WPRE /note="label=""WPRE"" - ApEinfo_revcolor=#d59687 - ApEinfo_fwdcolor=#d59687 - 5531..6118" /note="color: #a7a8a8; direction: RIGHT" primer_bind 3343..3362 /label=pCAG-C /note="color: #9b43df; direction: RIGHT" CDS complement(3678..3689) /label=FactorXa site /note="color: #a7a8a8; direction: LEFT" CDS complement(3678..3689) /label=FactorXa site /note="color: #008000; direction: LEFT" terminator complement(3835..3954) /label=SV40_PA_terminator /note="label=""SV40_PA_terminator"" - ApEinfo_revcolor=#f58a5e - ApEinfo_fwdcolor=#f58a5e - complement(6157..6276)" /note="color: #a7a8a8; direction: LEFT" misc_feature complement(3849..3868) /label=EBV_rev_primer /note="label=""EBV_rev_primer"" - ApEinfo_revcolor=#84b0dc - ApEinfo_fwdcolor=#84b0dc - complement(6171..6190)" /note="color: #9b43df; direction: LEFT" misc_feature complement(4083..4250) /label=R-ITR /note="color: #80ffff; direction: LEFT" rep_origin complement(4469..5097) /direction=LEFT /label=ColE1 origin /note="color: #a7a8a8" rep_origin complement(4487..5075) /direction=LEFT /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o... /note="direction=RIGHT - note=""ori"" - note=""high-copy-number ColE1/pMB1/pBR322/pUC origin of - replication"" - 2073..2661" /note="color: #a7a8a8" gene complement(5246..6106) /label=Ampicillin /note="label=""Ampicillin"" - ApEinfo_revcolor=#d6b295 - ApEinfo_fwdcolor=#d6b295 - complement(941..1801)" /note="color: #a7a8a8; direction: LEFT" CDS complement(5246..6106) /label=confers resistance to ampicillin, carbenicillin... /note="codon_start=1 - gene=""bla"" - product=""beta-lactamase"" - note=""AmpR"" - note=""confers resistance to ampicillin, carbenicillin, and - related antibiotics"" - translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI - ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAG" /note="color: #a7a8a8; direction: LEFT" CDS complement(5249..5908) /label=AmpR /note="color: #a7a8a8; direction: LEFT" promoter complement(6107..6211) /label=AmpR promoter_bla_promoter /note="gene=""bla"" - note=""AmpR promoter"" - 937..1041" /note="color: #a7a8a8; direction: LEFT" promoter complement(6148..6176) /label=AmpR_promoter /note="label=""AmpR_promoter"" - ApEinfo_revcolor=#b1ff67 - ApEinfo_fwdcolor=#b1ff67 - complement(1843..1871)" /note="color: #a7a8a8; direction: LEFT" rep_origin complement(6237..6692) /direction=LEFT /label=f1 bacteriophage origin of replication; arrow _... /note="direction=RIGHT - note=""f1 ori"" - note=""f1 bacteriophage origin of replication; arrow - indicates direction of (+) strand synthesis"" - 456..911" /note="color: #a7a8a8" rep_origin 6247..6687 /direction=RIGHT /label=F1 ori /note="color: #a7a8a8" CDS complement(6694..6762) /label=LacZ alpha /note="color: #a7a8a8; direction: LEFT" misc_feature 6764..6931 /label=L-ITR /note="color: #80ffff; direction: RIGHT" ORIGIN 1 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 61 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 121 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 181 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 241 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc 301 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg 361 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg 421 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg 481 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg 541 acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg 601 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa 661 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg 721 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct 781 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta 841 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc 901 tggttattgt gctgtctcat cattttggca aagaattgga tccccataac ttcgtataaa 961 gtatcctata cgaagttata tcaaaatagg aagaccaatg cttcaccatc gacccgaatt 1021 gccaagcatc accatcgacc cataacttcg tataatgtat gctatacgaa gttatactag 1081 ggatcccctt acttgtacag agctcggcgc gcctcgagct acagggctac ctcattttca 1141 tagcaaaagg agttagcgtt ctcctggcgt cgcctttccc gcctcttctg caagtatttg 1201 atgaggatga taagggagat gatggtgagc accaccaggg ccaggatggc tgagatcacc 1261 accaccttaa agggcaagga gtgtggcacc acgatgacct cctgcgtgtc ctggcccaca 1321 gcattcagat cctcttctga gatgagtttt tgttcacgcg taattaaacc tttctctttc 1381 aaaaactcct tagctacttt cgctgggtcc tgaccctcac cgtcacgctt gtaattcagt 1441 ttctgcattt cttccagtga aatttgattc gcgagtttgt tcagcacgtc cttcagttca 1501 ggatgcttat ctaagacatc ctgacggatg atgggggcag cataatacgg cgggaagaac 1561 gctttatcat cctccagaat ttttaatttg tggctcacca gcaagccatc agtggcagtg 1621 gcatcaatta cctgaacttc gttattatca atggcggtat acggaatacc agcgtccatg 1681 cttttggtgt gcttgaattt gaaattgtac agtttttgca ggcctgggta accatcggga 1741 ttttctaaaa acatcatgtt tgcacccaga atcagcttat ccgagatttt cgctaagtca 1801 ctgaaggttt taaggttata ctgtttcgcc agttcgtctt taaccgcaag cacatacgaa 1861 gcgttgaatc ccagtggttt gagccataca atattccatt tacgtttgta ctccttcttc 1921 acggtttcgt atgcaccttc tggatcagta gcaccgggcg gcggaaagtt gtactccagc 1981 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc 2041 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtaggtgcc gtcgtccttg 2101 aagctgatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc 2161 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg 2221 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg 2281 ccgttggtgg catcgccctc gccctcgccg cgcacgctga acttgtggcc gtttacgccg 2341 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcatg 2401 ctccctccgg taccgccctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc 2461 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagcacggac 2521 tgggtgctca ggtagtggtt gtcaggcagc agcacagggc cgtcgccgat gggggtgttc 2581 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcca cgttgtagcg gatcttgaag 2641 ttcgccttga tgccgttctt ctgcttgtcg gcgatgatat agacgttcgg ctccgggtac 2701 cccagaatat ccacaagacc atacccaacg tactccaggt aaatgtcaat accattattc 2761 gcacctccgc gtttaatggc ttcaaagtta acgttaaccc cgccaaggtt cagtttgcga 2821 accaccttaa ggtctgtatg cgcctcaatc atctctgcca acatgtttgc gacgataatc 2881 tgttctgtat ggttaatcga gcccacaact acggtgtcgt tcgcagatct gtcaccagtg 2941 gaacctggaa cccagagcag cagtacccat agcaggagtg tgtctgtctc catggtggca 3001 ctagtgctag cggtaccgaa ttctagtata acttcgtata ggatacttta tacgaagtta 3061 tcattgggat tcttcctatt ttgatccaag catcaccatc gaccctctag tccagatctc 3121 accatcgacc cataacttcg tatagcatac attatacgaa gttatgtccc tcgaagaggt 3181 tcgaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat 3241 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 3301 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 3361 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 3421 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 3481 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 3541 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 3601 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 3661 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 3721 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 3781 gggccgcctc cccgcatcga taccgtcgac ccgggcggcc gcttcgagca gacatgataa 3841 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 3901 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 3961 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt 4021 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatcttcct agagcatggc 4081 tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag 4141 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 4201 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcattaat 4261 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 4321 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 4381 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 4441 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 4501 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 4561 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 4621 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 4681 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 4741 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 4801 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 4861 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 4921 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 4981 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 5041 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 5101 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 5161 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 5221 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 5281 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 5341 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 5401 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 5461 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 5521 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 5581 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 5641 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 5701 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 5761 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 5821 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 5881 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 5941 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 6001 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 6061 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 6121 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 6181 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat 6241 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt 6301 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg 6361 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt 6421 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc 6481 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg 6541 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa 6601 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc 6661 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg 6721 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctgcgcg ctcgctcgct 6781 cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt 6841 gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt ccttgtagtt 6901 aatgattaac ccgccatgct acttatctac gtagccatgc tctaggaaga tcgta //