LOCUS       Exported                6955 bp ds-DNA     circular SYN 24-JUN-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 24, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
COMMENT     Ligation of : pAAV-CAG-FLEX-YMAT-PDGFR-3.xdna [5064 nt] : 
            (SpeI[2952] / XhoI[1113]) to PCR(pMinDis-L310E10-PDGFR-EnhExp.xdna) 
            [1883 nt] : (XhoI[1891] / SpeI[4])
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..249
                     /label=CMV Enhancer
                     /note="color: #84a4c0; direction: RIGHT"
     misc_feature    251..528
                     /label=Chicken B-actin Promoter
                     /note="color: #92abc4; direction: RIGHT"
     primer_bind     808..830
                     /label=CAG-F
                     /note="color: #9b43df; direction: RIGHT"
     misc_feature    892..911
                     /label=pCAG_F_primer
                     /note="label=""pCAG_F_primer"" - ApEinfo_revcolor=#f58a5e -
                     ApEinfo_fwdcolor=#f58a5e - 3542..3561"
                     /note="color: #9b43df; direction: RIGHT"
     misc_feature    946..979
                     /label=lox2722
                     /note="label=""loxP"" - ApEinfo_revcolor=#9eafd2 - 
                     ApEinfo_fwdcolor=#9eafd2 - 3596..3629"
                     /note="color: #1da0ff; direction: RIGHT"
     misc_feature    1042..1075
                     /label=LoxP
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    complement(1122..1199)
                     /label=ProposedExportMotifs_perLL_123016_misc_feature
                     /note="note=""ProposedExportMotifs_perLL_123016"" - 
                     975..1052"
                     /note="color: #0378ff; direction: LEFT"
     misc_feature    complement(1191..1259)
                     /label=PDGFR transmembrane helix_misc_feature
                     /note="note=""PDGFR transmembrane helix"" - 915..983"
                     /note="color: #529cff; direction: LEFT"
     CDS             complement(1326..1355)
                     /label=c-Myc tag
                     /note="color: #008000; direction: LEFT"
     CDS             complement(1326..1355)
                     /label=Myc_Myc (human c-Myc oncogene) epitope tag_CDS
                     /note="codon_start=1 - note=""Myc (human c-Myc oncogene) 
                     epitope tag"" - note=""Myc"" - translation=""EQKLISEEDL"" -
                     819..848"
                     /note="color: #f08080; direction: LEFT"
     misc_feature    complement(1362..1955)
                     /label=X513OpuB 107-305
                     /note="note=""X513OpuB 107-305"" - 1774..2367"
                     /note="color: #dc143c; direction: LEFT"
     misc_feature    complement(1956..1964)
                     /label=Linker (C)
                     /note="color: #0378ff; direction: LEFT"
     misc_feature    complement(1965..2687)
                     /label=cpsfGFP
                     /note="color: #5fff45; direction: LEFT"
     misc_feature    complement(2688..2699)
                     /label=Linker (N)
                     /note="color: #0378ff; direction: LEFT"
     misc_feature    complement(2700..2924)
                     /label=X513OpuB 30-106
                     /note="note=""X513OpuB 30-106"" - 805..1029"
                     /note="color: #dc143c; direction: LEFT"
     misc_feature    complement(2925..2930)
                     /label=MCS_misc_feature
                     /note="note=""MCS"" - 799..804"
                     /note="color: #b5978d; direction: LEFT"
     CDS             complement(2931..2993)
                     /label=Ig-kappa leader_leader sequence from mouse_CDS
                     /note="codon_start=1 - note=""leader sequence from mouse"" 
                     - note=""Ig-kappa leader"" - 
                     translation=""METDTLLLWVLLLWVPGSTGD"" - 737..799"
                     /note="color: #f08080; direction: LEFT"
     misc_feature    3028..3061
                     /label=lox2722
                     /note="color: #1792ff; direction: RIGHT"
     misc_recomb     3132..3165
                     /label=LoxP
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    3209..3796
                     /label=WPRE
                     /note="label=""WPRE"" - ApEinfo_revcolor=#d59687 - 
                     ApEinfo_fwdcolor=#d59687 - 5531..6118"
                     /note="color: #a7a8a8; direction: RIGHT"
     primer_bind     3343..3362
                     /label=pCAG-C
                     /note="color: #9b43df; direction: RIGHT"
     CDS             complement(3678..3689)
                     /label=FactorXa site
                     /note="color: #a7a8a8; direction: LEFT"
     CDS             complement(3678..3689)
                     /label=FactorXa site
                     /note="color: #008000; direction: LEFT"
     terminator      complement(3835..3954)
                     /label=SV40_PA_terminator
                     /note="label=""SV40_PA_terminator"" - 
                     ApEinfo_revcolor=#f58a5e - ApEinfo_fwdcolor=#f58a5e - 
                     complement(6157..6276)"
                     /note="color: #a7a8a8; direction: LEFT"
     misc_feature    complement(3849..3868)
                     /label=EBV_rev_primer
                     /note="label=""EBV_rev_primer"" - ApEinfo_revcolor=#84b0dc 
                     - ApEinfo_fwdcolor=#84b0dc - complement(6171..6190)"
                     /note="color: #9b43df; direction: LEFT"
     misc_feature    complement(4083..4250)
                     /label=R-ITR
                     /note="color: #80ffff; direction: LEFT"
     rep_origin      complement(4469..5097)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #a7a8a8"
     rep_origin      complement(4487..5075)
                     /direction=LEFT
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o...
                     /note="direction=RIGHT - note=""ori"" - 
                     note=""high-copy-number ColE1/pMB1/pBR322/pUC origin of - 
                     replication"" - 2073..2661"
                     /note="color: #a7a8a8"
     gene            complement(5246..6106)
                     /label=Ampicillin
                     /note="label=""Ampicillin"" - ApEinfo_revcolor=#d6b295 - 
                     ApEinfo_fwdcolor=#d6b295 - complement(941..1801)"
                     /note="color: #a7a8a8; direction: LEFT"
     CDS             complement(5246..6106)
                     /label=confers resistance to ampicillin, carbenicillin...
                     /note="codon_start=1 - gene=""bla"" - 
                     product=""beta-lactamase"" - note=""AmpR"" - note=""confers
                     resistance to ampicillin, carbenicillin, and - related 
                     antibiotics"" - 
                     translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                      - ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAG"
                     /note="color: #a7a8a8; direction: LEFT"
     CDS             complement(5249..5908)
                     /label=AmpR
                     /note="color: #a7a8a8; direction: LEFT"
     promoter        complement(6107..6211)
                     /label=AmpR promoter_bla_promoter
                     /note="gene=""bla"" - note=""AmpR promoter"" - 937..1041"
                     /note="color: #a7a8a8; direction: LEFT"
     promoter        complement(6148..6176)
                     /label=AmpR_promoter
                     /note="label=""AmpR_promoter"" - ApEinfo_revcolor=#b1ff67 -
                     ApEinfo_fwdcolor=#b1ff67 - complement(1843..1871)"
                     /note="color: #a7a8a8; direction: LEFT"
     rep_origin      complement(6237..6692)
                     /direction=LEFT
                     /label=f1 bacteriophage origin of replication; arrow _...
                     /note="direction=RIGHT - note=""f1 ori"" - note=""f1 
                     bacteriophage origin of replication; arrow - indicates 
                     direction of (+) strand synthesis"" - 456..911"
                     /note="color: #a7a8a8"
     rep_origin      6247..6687
                     /direction=RIGHT
                     /label=F1 ori
                     /note="color: #a7a8a8"
     CDS             complement(6694..6762)
                     /label=LacZ alpha
                     /note="color: #a7a8a8; direction: LEFT"
     misc_feature    6764..6931
                     /label=L-ITR
                     /note="color: #80ffff; direction: RIGHT"
ORIGIN
        1 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
       61 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      121 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      181 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      241 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
      301 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
      361 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
      421 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
      481 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
      541 acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      601 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
      661 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      721 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
      781 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
      841 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
      901 tggttattgt gctgtctcat cattttggca aagaattgga tccccataac ttcgtataaa
      961 gtatcctata cgaagttata tcaaaatagg aagaccaatg cttcaccatc gacccgaatt
     1021 gccaagcatc accatcgacc cataacttcg tataatgtat gctatacgaa gttatactag
     1081 ggatcccctt acttgtacag agctcggcgc gcctcgagct acagggctac ctcattttca
     1141 tagcaaaagg agttagcgtt ctcctggcgt cgcctttccc gcctcttctg caagtatttg
     1201 atgaggatga taagggagat gatggtgagc accaccaggg ccaggatggc tgagatcacc
     1261 accaccttaa agggcaagga gtgtggcacc acgatgacct cctgcgtgtc ctggcccaca
     1321 gcattcagat cctcttctga gatgagtttt tgttcacgcg taattaaacc tttctctttc
     1381 aaaaactcct tagctacttt cgctgggtcc tgaccctcac cgtcacgctt gtaattcagt
     1441 ttctgcattt cttccagtga aatttgattc gcgagtttgt tcagcacgtc cttcagttca
     1501 ggatgcttat ctaagacatc ctgacggatg atgggggcag cataatacgg cgggaagaac
     1561 gctttatcat cctccagaat ttttaatttg tggctcacca gcaagccatc agtggcagtg
     1621 gcatcaatta cctgaacttc gttattatca atggcggtat acggaatacc agcgtccatg
     1681 cttttggtgt gcttgaattt gaaattgtac agtttttgca ggcctgggta accatcggga
     1741 ttttctaaaa acatcatgtt tgcacccaga atcagcttat ccgagatttt cgctaagtca
     1801 ctgaaggttt taaggttata ctgtttcgcc agttcgtctt taaccgcaag cacatacgaa
     1861 gcgttgaatc ccagtggttt gagccataca atattccatt tacgtttgta ctccttcttc
     1921 acggtttcgt atgcaccttc tggatcagta gcaccgggcg gcggaaagtt gtactccagc
     1981 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
     2041 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtaggtgcc gtcgtccttg
     2101 aagctgatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
     2161 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg
     2221 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
     2281 ccgttggtgg catcgccctc gccctcgccg cgcacgctga acttgtggcc gtttacgccg
     2341 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcatg
     2401 ctccctccgg taccgccctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
     2461 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagcacggac
     2521 tgggtgctca ggtagtggtt gtcaggcagc agcacagggc cgtcgccgat gggggtgttc
     2581 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcca cgttgtagcg gatcttgaag
     2641 ttcgccttga tgccgttctt ctgcttgtcg gcgatgatat agacgttcgg ctccgggtac
     2701 cccagaatat ccacaagacc atacccaacg tactccaggt aaatgtcaat accattattc
     2761 gcacctccgc gtttaatggc ttcaaagtta acgttaaccc cgccaaggtt cagtttgcga
     2821 accaccttaa ggtctgtatg cgcctcaatc atctctgcca acatgtttgc gacgataatc
     2881 tgttctgtat ggttaatcga gcccacaact acggtgtcgt tcgcagatct gtcaccagtg
     2941 gaacctggaa cccagagcag cagtacccat agcaggagtg tgtctgtctc catggtggca
     3001 ctagtgctag cggtaccgaa ttctagtata acttcgtata ggatacttta tacgaagtta
     3061 tcattgggat tcttcctatt ttgatccaag catcaccatc gaccctctag tccagatctc
     3121 accatcgacc cataacttcg tatagcatac attatacgaa gttatgtccc tcgaagaggt
     3181 tcgaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     3241 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     3301 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     3361 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     3421 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     3481 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     3541 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     3601 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     3661 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     3721 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     3781 gggccgcctc cccgcatcga taccgtcgac ccgggcggcc gcttcgagca gacatgataa
     3841 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     3901 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     3961 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
     4021 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatcttcct agagcatggc
     4081 tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag
     4141 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     4201 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcattaat
     4261 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4321 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4381 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4441 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4501 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4561 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4621 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4681 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4741 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4801 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4861 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4921 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4981 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5041 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5101 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5161 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5221 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5281 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5341 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5401 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5461 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5521 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5581 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5641 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5701 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5761 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5821 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5881 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5941 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6001 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6061 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6121 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6181 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     6241 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     6301 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     6361 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     6421 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     6481 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     6541 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     6601 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     6661 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     6721 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctgcgcg ctcgctcgct
     6781 cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt
     6841 gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt ccttgtagtt
     6901 aatgattaac ccgccatgct acttatctac gtagccatgc tctaggaaga tcgta
//
