LOCUS       Exported               10685 bp ds-DNA     circular SYN 08-OCT-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 8, 2019 from SnapGene 5.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            61..967
                     /label=HO-L taken from 5' upstream region of
                     /note="HO-L taken from 5' upstream region of"
                     /note="HO-L taken from 5' upstream region of;HO-L taken
                     from 5' upstream region of HO -1815 to -2721"
                     /note="color: #ffc0cb; direction: RIGHT"
     misc_feature    complement(281..300)
                     /label=Primer 6 on 5' end of HO-L
                     /label=Primer 6 on 5' end of HO-L; used for
                     /note="Primer 6 on 5' end of HO-L"
                     /note="Primer 6 on 5' end of HO-L; used for"
                     /note="color: #ffc0cb; direction: LEFT"
     promoter        986..1485
                     /label=Sup35 promoter
                     /note="Sup35 promoter"
                     /note="color: #ffc0cb"
     protein_bind    1498..1622
                     /gene="mutant version of attR"
                     /label=mutant version of attR
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #ffc0cb"
     misc_recomb     1498..1622
                     /label=attR1
                     /note="attR1"
                     /note="color: #ffc0cb"
     promoter        1647..1677
                     /label=lac UV5 promoter
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
                     /note="color: #ffc0cb"
     CDS             1731..2390
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /note="color: #ffc0cb"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    1731..2390
                     /label=CAT/CamR
                     /note="CAT/CamR"
                     /note="color: #ffc0cb"
     CDS             2732..3037
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=CcdB, a bacterial toxin that poisons DNA gyrase
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be
                     propagated in standard E. coli strains."
                     /note="color: #ffc0cb"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    2732..3037
                     /label=ccdB
                     /note="ccdB"
                     /note="color: #ffc0cb"
     protein_bind    complement(3078..3202)
                     /gene="mutant version of attR"
                     /label=mutant version of attR
                     /label=mutant version of attR(1)
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_recomb     3078..3194
                     /label=attR2
                     /note="attR2"
                     /note="color: #ffc0cb"
     misc_feature    3195..3233
                     /label=Space between attR1 site
                     /note="Space between attR1 site"
                     /note="color: #ffc0cb"
     CDS             3234..3278
                     /label=NLS
                     /note="NLS"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             3240..3260
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=nuclear localization signal of SV40 large T  antig
                     /note="SV40 NLS"
                     /note="color: #ffc0cb"
                     /translation="PKKKRKV"
     CDS             3291..3524
                     /label=VP16 Activation Domain
                     /note="VP16 Activation Domain"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             3537..3797
                     /label=ZF
                     /note="ZF"
                     /note="color: #0000ff; direction: RIGHT"
     CDS             3813..3842
                     /label=NES
                     /note="NES"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             3864..4055
                     /label=6x HA
                     /note="6x HA"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             3864..3953
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=three tandem HA epitope tags
                     /note="3xHA"
                     /note="color: #ffc0cb"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             3960..4049
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=three tandem HA epitope tags
                     /label=three tandem HA epitope tags(1)
                     /note="3xHA"
                     /note="color: #ffc0cb"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      4075..4313
                     /label=Sup35 Terminator
                     /note="Sup35 Terminator"
                     /note="color: #ffc0cb"
     misc_signal     4472..4683
                     /label=8x-43-8
                     /note="8x-43-8"
                     /note="color: #ffc0cb"
     promoter        4693..4926
                     /label=Minimal CYC1 promoter
                     /note="Minimal CYC1 promoter"
                     /note="color: #ffc0cb"
     CDS             4927..4939
                     /label=ORF1
                     /note="ORF1"
                     /note="color: #ffc0cb; direction: RIGHT"
     misc_feature    4927..4934
                     /label=PmeI site
                     /note="PmeI site"
                     /note="color: #ffc0cb"
     protein_bind    4935..4939
                     /label=RBS
                     /note="RBS"
                     /note="color: #ffc0cb"
     CDS             4940..5638
                     /codon_start=1
                     /label=mNeonGreen
                     /note="mNeonGreen"
                     /note="mNeonGreen;http://www.ncbi.nlm.nih.gov/protein/4593
                     6 0587?repor t =genbank"
                     /note="color: #ffc0cb"
                     /translation="MGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELN
                     LKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGAS
                     LTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFK
                     WSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAF
                     TDVMGMDELYK"
     primer_bind     4940..4963
                     /label=DML_P218_F
                     /note="DML_P218_F"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             5546..5548
                     /codon_start=1
                     /label=A206K
                     /note="A206K"
                     /note="color: #ffc0cb"
                     /translation="K"
     misc_feature    complement(5617..5638)
                     /label=DML_P219_R
                     /note="DML_P219_R"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    5618..5638
                     /label=EGFP-type terminus
                     /note="EGFP-type terminus"
                     /note="color: #ffc0cb"
     protein_bind    5642..5649
                     /label=AscI
                     /note="AscI"
                     /note="color: #ffc0cb"
     terminator      5662..5849
                     /gene="S. cerevisiae ADH1"
                     /label=S. cerevisiae ADH1
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase
                     1"
                     /note="color: #ffc0cb"
     terminator      5662..5841
                     /label=yADH1_terminator
                     /note="yADH1_terminator"
                     /note="color: #ffc0cb"
     misc_feature    6075..6094
                     /label=kanB
                     /note="kanB"
                     /note="color: #ffc0cb"
     CDS             6274..6846
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=nourseothricin acetyltransferase
                     /note="NrsR"
                     /note="confers resistance to nourseothricin"
                     /note="color: #ffc0cb"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     CDS             6274..6280
                     /codon_start=1
                     /label=KanR2(variant)
                     /note="KanR2(variant)"
                     /note="KanR2(variant);ORF frame 3"
                     /note="color: #ffc0cb"
                     /translation="MG"
     CDS             6281..6846
                     /label=NATr
                     /note="NATr"
                     /note="color: #ffc0cb; direction: RIGHT"
     terminator      6858..7055
                     /label=TEF terminator
                     /note="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
                     /note="color: #ffc0cb"
     misc_feature    complement(7094..7103)
                     /label=R2
                     /note="R2"
                     /note="color: #ffc0cb; direction: LEFT"
     gene            7109..7609
                     /label=HO-R matches 1190 of gene to 1690
                     /note="HO-R matches 1190 of gene to 1690"
                     /note="color: #ffc0cb; direction: RIGHT"
     primer_bind     7495..7514
                     /label=PCR primer 11 for 3' end of HO-R
                     /note="PCR primer 11 for 3' end of HO-R"
                     /note="color: #ffc0cb; direction: RIGHT"
     misc_feature    7617..7618
                     /label=NotI(1)
                     /note="NotI(1)"
                     /note="NotI"
                     /note="color: #ffc0cb"
     primer_bind     7619..7634
                     /label=SK primer
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #ffc0cb; direction: RIGHT"
     promoter        complement(7649..7667)
                     /label=T7 promoter
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffc0cb; direction: LEFT"
     primer_bind     complement(7674..7690)
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #ffc0cb; direction: LEFT"
     promoter        8562..8666
                     /gene="bla"
                     /label=bla
                     /note="AmpR promoter"
                     /note="color: #ffc0cb"
     CDS             8667..9527
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="color: #ffc0cb"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9698..10286
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffc0cb"
     protein_bind    10574..10595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #ffc0cb"
     protein_bind    10650..10666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #ffc0cb"
     primer_bind     join(10674..10685,1..5)
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #ffc0cb; direction: RIGHT"
ORIGIN
        1 atgaccatga ttacgccact agtccgaggc ctcgagatcc gatatcgccg tggcggccgc
       61 aattatcctg ggcacgagtg aaacaaagct aaaaccttta tttagcatgg ccattgaatg
      121 taacaattat atatatcgca agcacaaaaa atcaaggaga gagaactacc actttgttca
      181 tgtgtacaat gttcattatc tccataagca aaaaaaaaaa aatagaaaac atatgctata
      241 aggttgatat tctcacgagt aagcggcact tgctacttat tgacattgca gatttttggc
      301 tacagaaata gtatattaga gattataatt gctaatcaaa tcaaaatata aaattagtaa
      361 accaaaccat ttataccctt ccttagtagt tatggattgt tttttaatga tatttctgca
      421 aaccaaagaa agattgttat ccagatagaa tttagttttg atattcattt ttttgttgaa
      481 gattgaacgc catatctggg cctcataatt caaaagacgg tgccattatc ggtagcgttt
      541 cgcattgtac tggatttcag aaatttcaca gttgatgaat cgaaaagaat ggtctcattg
      601 caacacgtaa ggttaagatg tcccttttta ccattatagg caataaatga atcataaaac
      661 gaccgtatac tggtgaaata gtagggagaa cgagtacctg tagtaaaaag tataaatcat
      721 agttaatcgg gcaatgtccc tcgatcaagg agtattgtgt catgttcgag acaaacgcca
      781 acatttttgt ttcttttgga caaatgttgt ttgcatttat gatccgttat attttgatct
      841 aatgtagagt tgcacgtagt tcttactggc aaagaaatcg atgcatacca aaaaagaata
      901 aaggtgatat ttgatcttta ccgtttagtt ccaacgtaaa attgtgcctt tggacttaaa
      961 atggcgtcgt acgctgcacc gagtgcaacc acacaaaaat catacaacga atggtatatg
     1021 cttcatttct ttgtttcgca ttagctgcgc tatttgactc aaattattat tttttactaa
     1081 gacgacgcgt cacagtgttc gagtctgtgt catttctttt gtaattctct taaaccactt
     1141 cataaagttg tgaagttcat agcaaaattc ttccgcaaaa agatgaatct tagttctcag
     1201 cccaccaaaa gaggtacatg ctaagatcat acagaagtta ttgtcacttc ttaccttgct
     1261 cttaaatgta cattacaacc gggtattata tcttacatca tcgtataata tgatctttct
     1321 ttatggagaa aatttttttt tcactcgacc aaagctccca ttgcttctga agagtgtagt
     1381 gtatattggt acatcttctc ttgaaagact ccattgtact gtaacaaaaa gcggtttctt
     1441 catcgacttg ctcggaataa catctatatc tgcccactag caacagctag ccccatcaca
     1501 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
     1561 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
     1621 tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     1681 ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa
     1741 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
     1801 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
     1861 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
     1921 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
     1981 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     2041 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     2101 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     2161 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     2221 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     2281 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
     2341 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat
     2401 ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa
     2461 gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcaa tgaagcagcg
     2521 tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat
     2581 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc
     2641 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc
     2701 tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag
     2761 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg
     2821 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact
     2881 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag
     2941 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat
     3001 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag
     3061 ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct
     3121 gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt
     3181 cagctttctt gtacaaagtg gtgatgggct gcaggaattc gatatcaagc ttaatggctc
     3241 caaagaagaa gagaaaggtt ggtattcatg gtgttccagt tgatggtggt ttacaagctc
     3301 caccaactga tgtttctttg ggtgatgaat tgcatttgga tggtgaagat gttgctatgg
     3361 ctcatgctga tgctttggat gattttgatt tggatatgtt gggtgacggt gattctccag
     3421 gtccaggttt tactccacat gattctgctc catatggtgc tttagatatg gctgactttg
     3481 aattcgaaca aatgttcacc gatgccttgg gtattgatga atatggtggt ggtgctagac
     3541 caggtgaaag accatttcaa tgtagaatct gcatgagaaa cttctccaga caagatagat
     3601 tggatagaca taccagaact catactggtg aaaagccatt ccaatgcaga atatgtatga
     3661 gaaatttttc ccaaaaagaa cacttggccg gtcatttgag aacacacaca ggtgaaaaac
     3721 cttttcaatg cagaatatgc atgagaaact ttagtagaag agacaacttg aacagacact
     3781 tgaaaaccca tttgagaggt tctgccggcg aattggcttt gaaattggct ggtttggata
     3841 tcaattctgg ttctgctgct agatacccat atgatgttcc agattatgct ggttaccctt
     3901 acgatgttcc tgactacgca ggttcttatc catacgatgt acctgattat gcctcaagat
     3961 atccttatga cgtccctgat tacgccggtt atccatatga cgtaccagac tacgctggta
     4021 gttacccata cgacgtccca gattacgctt cttcataatg agggccctca tgtaatttct
     4081 tgcaaacata agtaaatgca aacacaataa taccgatcat aaagcatttt cttctatatt
     4141 aaaaaacaag gtttaataaa gctgttatat atatatatat atatatagac gtataattag
     4201 tttagttctt tttgtaccat ataccataaa caaggtaaac ttcacctctc aatatatcta
     4261 gaatttcata aaaatatcta gcaaggtttc aactccttca atcacgtttt catcactcaa
     4321 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4381 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     4441 aatttcctga tgcggtattt tctttaatta aagagtgagg actcgaaaat attaatagag
     4501 tgaggactcg aaaatattaa tagagtgagg actcgaaaat attaatagag tgaggactcg
     4561 aaaatattaa tatcgataga gtgaggactc gaaaatatta atagagtgag gactcgaaaa
     4621 tattaatgaa ttcagagtga ggactcgaaa atattaatag agtgaggact cgaaaatatt
     4681 aatggccggc ccagatccgc caggcgtgta tatatagcgt ggatggccag gcaactttag
     4741 tgctgacaca tacaggcata tatatatgtg tgcgacgaca catgatcata tggcatgcat
     4801 gtgctctgta tgtatataaa actcttgttt tcttcttttc tctaaatatt ctttccttat
     4861 acattaggac ctttgcagca taaattacta tacttctata gacacacaaa cacaaataca
     4921 cacactgttt aaacaaaaaa tgggcgagga ggataacatg gcctctctcc cagcgacaca
     4981 tgagttacac atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac
     5041 cggcaatcca aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca
     5101 gttctccccc tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta
     5161 ccctgacggg atgtcgcctt tccaggccgc catggtagat ggctccggat accaagtcca
     5221 tcgcacaatg cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga
     5281 gggaagccac atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc
     5341 tgtgatgacc aactcgctga ccgctgcgga ctggtgcagg tcgaagaaga cttaccccaa
     5401 cgacaaaacc atcatcagta cctttaagtg gagttacacc actggaaatg gcaagcgcta
     5461 ccggagcact gcgcggacca cctacacctt tgccaagcca atggcggcta actatctgaa
     5521 gaaccagccg atgtacgtgt tccgtaagac ggagctcaag cactccaaga ccgagctcaa
     5581 cttcaaggag tggcaaaagg cctttaccga tgtgatgggc atggacgagc tgtacaagta
     5641 aggcgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa
     5701 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
     5761 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag
     5821 gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag ggtaatatag
     5881 atctgtttag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa
     5941 taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta
     6001 catttagccc atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac
     6061 ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct
     6121 cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga
     6181 tttgccactg aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc
     6241 tcacatcaca tccgaacatc aattgaaaca accatgggta ccactcttga cgacacggct
     6301 taccggtacc gcaccagtgt cccgggggac gccgaggcca tcgaggcact ggatgggtcc
     6361 ttcaccaccg acaccgtctt ccgcgtcacc gccaccgggg acggcttcac cctgcgggag
     6421 gtgccggtgg acccgcccct gaccaaggtg ttccccgacg acgaatcgga cgacgaatcg
     6481 gacgacgggg aggacggcga cccggactcc cggacgttcg tcgcgtacgg ggacgacggc
     6541 gacctggcgg gcttcgtggt cgtctcgtac tccggctgga accgccggct gaccgtcgag
     6601 gacatcgagg tcgccccgga gcaccggggg cacggggtcg ggcgcgcgtt gatggggctc
     6661 gcgacggagt tcgcccgcga gcggggcgcc gggcacctct ggctggaggt caccaacgtc
     6721 aacgcaccgg cgatccacgc gtaccggcgg atggggttca ccctctgcgg cctggacacc
     6781 gccctgtacg acggcaccgc ctcggacggc gagcaggcgc tctacatgag catgccctgc
     6841 ccctaaagat cttcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg
     6901 tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt
     6961 tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat
     7021 gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc
     7081 atccagtgtc gaaaacgagc tcgaattcct gggggaacaa cttcacagaa tgttttgtca
     7141 tattgtcgaa gtggtcacaa aacaagagaa gttccgccaa ttataaaaag ggaacccgta
     7201 tatttcagct tcacggatga tttccagggt gagagtactg tatatgggct tacgatagaa
     7261 ggccataaaa atttcttgct tggcaacaaa atagaagtga aatcatgtcg aggctgctgt
     7321 gtgggagaac agcataaaat atcacaaaaa aagaatctaa aacactgtgt tgcttgtccc
     7381 agaaagggaa tcaagtattt ttataaagat tggagtggta aaaatcgagt atgtgctaga
     7441 tgctatggaa gatacaaatt cagcggtcat cactgtataa attgcaagta tgtaccagaa
     7501 gcacgtgaag tgaaaaaggc aaaagacaaa ggcgaaaaat tgggcattac gcccgaaggt
     7561 ttgccagtta aaggaccaga gtgtataaaa tgtggcggaa tcttacagtg cggccgctct
     7621 agaactagtg gatcgatccc caattcgccc tatagtgagt cgtattacaa ttcactggcc
     7681 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     7741 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     7801 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     7861 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     7921 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     7981 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8041 gtcaagctct aaatcggggt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8101 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
     8161 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
     8221 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     8281 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     8341 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     8401 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     8461 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     8521 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8581 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8641 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8701 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8761 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8821 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8881 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     8941 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9001 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9061 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9121 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9181 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9241 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9301 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9361 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9421 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9481 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9541 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9601 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9661 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9721 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9781 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9841 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9901 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9961 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10021 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10081 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    10141 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10201 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10261 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10321 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10381 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10441 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10501 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    10561 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    10621 tgcttccgcg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    10681 cagct
//
