LOCUS       Exported               10251 bp ds-DNA     circular SYN 08-OCT-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 8, 2019 from SnapGene 5.0.1
            https://www.snapgene.com
COMMENT     Insert from pENTR_RNQ1 652 to 1899
            Backbone from GAN147-NAT-Sup-Neon-43-8 3196 to 1510
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..5
                     /label=M13 rev
                     /label=M13 rev(1)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #ffc0cb; direction: RIGHT"
     gene            61..967
                     /label=HO-L taken from 5' upstream region of
                     /note="HO-L taken from 5' upstream region of"
                     /note="HO-L taken from 5' upstream region of;HO-L taken
                     from 5' upstream region of HO -1815 to -2721"
                     /note="color: #ffc0cb; direction: RIGHT"
     misc_feature    complement(281..300)
                     /label=Primer 6 on 5' end of HO-L
                     /label=Primer 6 on 5' end of HO-L; used for
                     /note="Primer 6 on 5' end of HO-L"
                     /note="Primer 6 on 5' end of HO-L; used for"
                     /note="color: #ffc0cb; direction: LEFT"
     promoter        986..1485
                     /label=Sup35 promoter
                     /note="Sup35 promoter"
                     /note="color: #ffc0cb"
     protein_bind    1498..1505
                     /gene="mutant version of attR"
                     /label=mutant version of attR
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #ffc0cb"
     misc_recomb     1498..1505
                     /label=attR1
                     /note="attR1"
                     /note="color: #ffc0cb"
     misc_recomb     1506..1522
                     /label=attL1
                     /note="attL1"
                     /note="attL1;recognized as attL1"
                     /note="color: #ff0000"
     misc_feature    1523..1527
                     /label=Kozak sequence
                     /note="Kozak sequence"
                     /note="color: #80ffff"
     CDS             1528..2742
                     /label=RNQ1
                     /note="color: #ffbc9b; direction: RIGHT"
     misc_recomb     complement(2744..2753)
                     /label=attL2
                     /note="attL2"
                     /note="attL2 (c);recognized as attL2"
                     /note="color: #ff0000; direction: LEFT"
     protein_bind    complement(2754..2768)
                     /gene="mutant version of attR"
                     /label=mutant version of attR
                     /label=mutant version of attR(1)
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_recomb     2754..2760
                     /label=attR2
                     /note="attR2"
                     /note="color: #ffc0cb"
     misc_feature    2761..2799
                     /label=Space between attR1 site
                     /note="Space between attR1 site"
                     /note="color: #ffc0cb"
     CDS             2800..2844
                     /label=NLS
                     /note="NLS"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             2806..2826
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=nuclear localization signal of SV40 large T  antig
                     /note="SV40 NLS"
                     /note="color: #ffc0cb"
                     /translation="PKKKRKV"
     CDS             2857..3090
                     /label=VP16 Activation Domain
                     /note="VP16 Activation Domain"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             3103..3363
                     /label=ZF
                     /note="ZF"
                     /note="color: #0000ff; direction: RIGHT"
     CDS             3379..3408
                     /label=NES
                     /note="NES"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             3430..3621
                     /label=6x HA
                     /note="6x HA"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             3430..3519
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=three tandem HA epitope tags
                     /note="3xHA"
                     /note="color: #ffc0cb"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             3526..3615
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=three tandem HA epitope tags
                     /label=three tandem HA epitope tags(1)
                     /note="3xHA"
                     /note="color: #ffc0cb"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      3641..3879
                     /label=Sup35 Terminator
                     /note="Sup35 Terminator"
                     /note="color: #ffc0cb"
     misc_signal     4038..4249
                     /label=8x-43-8
                     /note="8x-43-8"
                     /note="color: #ffc0cb"
     promoter        4259..4492
                     /label=Minimal CYC1 promoter
                     /note="Minimal CYC1 promoter"
                     /note="color: #ffc0cb"
     CDS             4493..4505
                     /label=ORF1
                     /note="ORF1"
                     /note="color: #ffc0cb; direction: RIGHT"
     misc_feature    4493..4500
                     /label=PmeI site
                     /note="PmeI site"
                     /note="color: #ffc0cb"
     protein_bind    4501..4505
                     /label=RBS
                     /note="RBS"
                     /note="color: #ffc0cb"
     CDS             4506..5204
                     /codon_start=1
                     /label=mNeonGreen
                     /note="mNeonGreen"
                     /note="mNeonGreen;http://www.ncbi.nlm.nih.gov/protein/4593
                     6 0587?repor t =genbank"
                     /note="color: #ffc0cb"
                     /translation="MGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELN
                     LKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGAS
                     LTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFK
                     WSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAF
                     TDVMGMDELYK"
     primer_bind     4506..4529
                     /label=DML_P218_F
                     /note="DML_P218_F"
                     /note="color: #ffc0cb; direction: RIGHT"
     CDS             5112..5114
                     /codon_start=1
                     /label=A206K
                     /note="A206K"
                     /note="color: #ffc0cb"
                     /translation="K"
     misc_feature    complement(5183..5204)
                     /label=DML_P219_R
                     /note="DML_P219_R"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    5184..5204
                     /label=EGFP-type terminus
                     /note="EGFP-type terminus"
                     /note="color: #ffc0cb"
     protein_bind    5208..5215
                     /label=AscI
                     /note="AscI"
                     /note="color: #ffc0cb"
     terminator      5228..5415
                     /gene="S. cerevisiae ADH1"
                     /label=S. cerevisiae ADH1
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase
                     1"
                     /note="color: #ffc0cb"
     terminator      5228..5407
                     /label=yADH1_terminator
                     /note="yADH1_terminator"
                     /note="color: #ffc0cb"
     misc_feature    5641..5660
                     /label=kanB
                     /note="kanB"
                     /note="color: #ffc0cb"
     CDS             5840..6412
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=nourseothricin acetyltransferase
                     /note="NrsR"
                     /note="confers resistance to nourseothricin"
                     /note="color: #ffc0cb"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     CDS             5840..5846
                     /codon_start=1
                     /label=KanR2(variant)
                     /note="KanR2(variant)"
                     /note="KanR2(variant);ORF frame 3"
                     /note="color: #ffc0cb"
                     /translation="MG"
     CDS             5847..6412
                     /label=NATr
                     /note="NATr"
                     /note="color: #ffc0cb; direction: RIGHT"
     terminator      6424..6621
                     /label=TEF terminator
                     /note="TEF terminator"
                     /note="Ashbya gossypii TEF terminator"
                     /note="color: #ffc0cb"
     misc_feature    complement(6660..6669)
                     /label=R2
                     /note="R2"
                     /note="color: #ffc0cb; direction: LEFT"
     gene            6675..7175
                     /label=HO-R matches 1190 of gene to 1690
                     /note="HO-R matches 1190 of gene to 1690"
                     /note="color: #ffc0cb; direction: RIGHT"
     primer_bind     7061..7080
                     /label=PCR primer 11 for 3' end of HO-R
                     /note="PCR primer 11 for 3' end of HO-R"
                     /note="color: #ffc0cb; direction: RIGHT"
     misc_feature    7182..7183
                     /label=NotI(1)
                     /note="NotI(1)"
                     /note="NotI"
                     /note="color: #ffc0cb"
     primer_bind     7184..7200
                     /label=SK primer
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #ffc0cb; direction: RIGHT"
     promoter        complement(7215..7233)
                     /label=T7 promoter
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffc0cb; direction: LEFT"
     primer_bind     complement(7240..7256)
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #ffc0cb; direction: LEFT"
     promoter        8128..8232
                     /gene="bla"
                     /label=bla
                     /note="AmpR promoter"
                     /note="color: #ffc0cb"
     CDS             8233..9093
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="color: #ffc0cb"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9264..9852
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffc0cb"
     protein_bind    10140..10161
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /note="color: #ffc0cb"
     protein_bind    10216..10232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #ffc0cb"
     primer_bind     10240..10251
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #ffc0cb; direction: RIGHT"
ORIGIN
        1 atgaccatga ttacgccact agtccgaggc ctcgagatcc gatatcgccg tggcggccgc
       61 aattatcctg ggcacgagtg aaacaaagct aaaaccttta tttagcatgg ccattgaatg
      121 taacaattat atatatcgca agcacaaaaa atcaaggaga gagaactacc actttgttca
      181 tgtgtacaat gttcattatc tccataagca aaaaaaaaaa aatagaaaac atatgctata
      241 aggttgatat tctcacgagt aagcggcact tgctacttat tgacattgca gatttttggc
      301 tacagaaata gtatattaga gattataatt gctaatcaaa tcaaaatata aaattagtaa
      361 accaaaccat ttataccctt ccttagtagt tatggattgt tttttaatga tatttctgca
      421 aaccaaagaa agattgttat ccagatagaa tttagttttg atattcattt ttttgttgaa
      481 gattgaacgc catatctggg cctcataatt caaaagacgg tgccattatc ggtagcgttt
      541 cgcattgtac tggatttcag aaatttcaca gttgatgaat cgaaaagaat ggtctcattg
      601 caacacgtaa ggttaagatg tcccttttta ccattatagg caataaatga atcataaaac
      661 gaccgtatac tggtgaaata gtagggagaa cgagtacctg tagtaaaaag tataaatcat
      721 agttaatcgg gcaatgtccc tcgatcaagg agtattgtgt catgttcgag acaaacgcca
      781 acatttttgt ttcttttgga caaatgttgt ttgcatttat gatccgttat attttgatct
      841 aatgtagagt tgcacgtagt tcttactggc aaagaaatcg atgcatacca aaaaagaata
      901 aaggtgatat ttgatcttta ccgtttagtt ccaacgtaaa attgtgcctt tggacttaaa
      961 atggcgtcgt acgctgcacc gagtgcaacc acacaaaaat catacaacga atggtatatg
     1021 cttcatttct ttgtttcgca ttagctgcgc tatttgactc aaattattat tttttactaa
     1081 gacgacgcgt cacagtgttc gagtctgtgt catttctttt gtaattctct taaaccactt
     1141 cataaagttg tgaagttcat agcaaaattc ttccgcaaaa agatgaatct tagttctcag
     1201 cccaccaaaa gaggtacatg ctaagatcat acagaagtta ttgtcacttc ttaccttgct
     1261 cttaaatgta cattacaacc gggtattata tcttacatca tcgtataata tgatctttct
     1321 ttatggagaa aatttttttt tcactcgacc aaagctccca ttgcttctga agagtgtagt
     1381 gtatattggt acatcttctc ttgaaagact ccattgtact gtaacaaaaa gcggtttctt
     1441 catcgacttg ctcggaataa catctatatc tgcccactag caacagctag ccccatcaca
     1501 agtttgtaca aaaaagcagg ctacaaaatg gatacggata agttaatctc agaggctgag
     1561 tctcattttt ctcaaggaaa ccatgcagaa gctgttgcga agttgacatc cgcagctcag
     1621 tcgaacccca atgacgagca aatgtcaact attgaatcat taattcaaaa aatcgcagga
     1681 tacgtcatgg acaaccgtag tggtggtagt gacgcctcgc aagatcgtgc tgctggtggt
     1741 ggttcatctt ttatgaacac tttaatggca gactctaagg gttcttccca aacgcaacta
     1801 ggaaaactag ctttgttagc cacagtgatg acacactcat caaataaagg ttcttctaac
     1861 agagggtttg acgtagggac tgtcatgtca atgctaagtg gttctggcgg cgggagccaa
     1921 agtatgggtg cttccggcct ggctgccttg gcttctcaat tctttaagtc aggtaacaat
     1981 tcccaaggtc agggacaagg tcaaggtcaa ggtcaaggtc aaggacaagg tcaaggtcaa
     2041 ggttctttta ctgctttggc gtctttggct tcatctttca tgaattccaa caacaataat
     2101 cagcaaggtc aaaatcaaag ctccggtggt tcctcctttg gagcactggc ttctatggca
     2161 agctctttta tgcattccaa taataatcag aactccaaca atagtcaaca gggctataac
     2221 caatcctatc aaaacggtaa ccaaaatagt caaggttaca ataatcaaca gtaccaaggt
     2281 ggcaacggtg gttaccaaca acaacaggga caatctggtg gtgctttttc ctcattggcc
     2341 tccatggctc aatcttactt aggtggtgga caaactcaat ccaaccaaca gcaatacaat
     2401 caacaaggcc aaaacaacca gcagcaatac cagcaacaag gccaaaacta tcagcatcaa
     2461 caacagggtc agcagcagca acaaggccac tccagttcat tctcagcttt ggcttccatg
     2521 gcaagttcct acctgggcaa taactccaat tcaaattcga gttatggggg ccagcaacag
     2581 gctaatgagt atggtagacc gcaacagaat ggtcaacagc aatccaatga gtacggaaga
     2641 ccgcaatacg gcggaaacca gaactccaat ggacagcacg aatccttcaa tttttctggc
     2701 aacttttctc aacagaacaa taacggcaac cagaaccgct acaacccagc tttcttgtac
     2761 aaagtggtga tgggctgcag gaattcgata tcaagcttaa tggctccaaa gaagaagaga
     2821 aaggttggta ttcatggtgt tccagttgat ggtggtttac aagctccacc aactgatgtt
     2881 tctttgggtg atgaattgca tttggatggt gaagatgttg ctatggctca tgctgatgct
     2941 ttggatgatt ttgatttgga tatgttgggt gacggtgatt ctccaggtcc aggttttact
     3001 ccacatgatt ctgctccata tggtgcttta gatatggctg actttgaatt cgaacaaatg
     3061 ttcaccgatg ccttgggtat tgatgaatat ggtggtggtg ctagaccagg tgaaagacca
     3121 tttcaatgta gaatctgcat gagaaacttc tccagacaag atagattgga tagacatacc
     3181 agaactcata ctggtgaaaa gccattccaa tgcagaatat gtatgagaaa tttttcccaa
     3241 aaagaacact tggccggtca tttgagaaca cacacaggtg aaaaaccttt tcaatgcaga
     3301 atatgcatga gaaactttag tagaagagac aacttgaaca gacacttgaa aacccatttg
     3361 agaggttctg ccggcgaatt ggctttgaaa ttggctggtt tggatatcaa ttctggttct
     3421 gctgctagat acccatatga tgttccagat tatgctggtt acccttacga tgttcctgac
     3481 tacgcaggtt cttatccata cgatgtacct gattatgcct caagatatcc ttatgacgtc
     3541 cctgattacg ccggttatcc atatgacgta ccagactacg ctggtagtta cccatacgac
     3601 gtcccagatt acgcttcttc ataatgaggg ccctcatgta atttcttgca aacataagta
     3661 aatgcaaaca caataatacc gatcataaag cattttcttc tatattaaaa aacaaggttt
     3721 aataaagctg ttatatatat atatatatat atagacgtat aattagttta gttctttttg
     3781 taccatatac cataaacaag gtaaacttca cctctcaata tatctagaat ttcataaaaa
     3841 tatctagcaa ggtttcaact ccttcaatca cgttttcatc actcaaccct atctcggtct
     3901 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3961 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcctgatgcg
     4021 gtattttctt taattaaaga gtgaggactc gaaaatatta atagagtgag gactcgaaaa
     4081 tattaataga gtgaggactc gaaaatatta atagagtgag gactcgaaaa tattaatatc
     4141 gatagagtga ggactcgaaa atattaatag agtgaggact cgaaaatatt aatgaattca
     4201 gagtgaggac tcgaaaatat taatagagtg aggactcgaa aatattaatg gccggcccag
     4261 atccgccagg cgtgtatata tagcgtggat ggccaggcaa ctttagtgct gacacataca
     4321 ggcatatata tatgtgtgcg acgacacatg atcatatggc atgcatgtgc tctgtatgta
     4381 tataaaactc ttgttttctt cttttctcta aatattcttt ccttatacat taggaccttt
     4441 gcagcataaa ttactatact tctatagaca cacaaacaca aatacacaca ctgtttaaac
     4501 aaaaaatggg cgaggaggat aacatggcct ctctcccagc gacacatgag ttacacatct
     4561 ttggctccat caacggtgtg gactttgaca tggtgggtca gggcaccggc aatccaaatg
     4621 atggttatga ggagttaaac ctgaagtcca ccaagggtga cctccagttc tccccctgga
     4681 ttctggtccc tcatatcggg tatggcttcc atcagtacct gccctaccct gacgggatgt
     4741 cgcctttcca ggccgccatg gtagatggct ccggatacca agtccatcgc acaatgcagt
     4801 ttgaagatgg tgcctccctt actgttaact accgctacac ctacgaggga agccacatca
     4861 aaggagaggc ccaggtgaag gggactggtt tccctgctga cggtcctgtg atgaccaact
     4921 cgctgaccgc tgcggactgg tgcaggtcga agaagactta ccccaacgac aaaaccatca
     4981 tcagtacctt taagtggagt tacaccactg gaaatggcaa gcgctaccgg agcactgcgc
     5041 ggaccaccta cacctttgcc aagccaatgg cggctaacta tctgaagaac cagccgatgt
     5101 acgtgttccg taagacggag ctcaagcact ccaagaccga gctcaacttc aaggagtggc
     5161 aaaaggcctt taccgatgtg atgggcatgg acgagctgta caagtaaggc gcgccacttc
     5221 taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat
     5281 aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag
     5341 taactctttc ctgtaggtca ggttgctttc tcaggtatag tatgaggtcg ctcttattga
     5401 ccacacctct accggcagat ccgctaggga taacagggta atatagatct gtttagcttg
     5461 cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca
     5521 gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac
     5581 atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa
     5641 attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga
     5701 attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt
     5761 tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg
     5821 aacatcaatt gaaacaacca tgggtaccac tcttgacgac acggcttacc ggtaccgcac
     5881 cagtgtcccg ggggacgccg aggccatcga ggcactggat gggtccttca ccaccgacac
     5941 cgtcttccgc gtcaccgcca ccggggacgg cttcaccctg cgggaggtgc cggtggaccc
     6001 gcccctgacc aaggtgttcc ccgacgacga atcggacgac gaatcggacg acggggagga
     6061 cggcgacccg gactcccgga cgttcgtcgc gtacggggac gacggcgacc tggcgggctt
     6121 cgtggtcgtc tcgtactccg gctggaaccg ccggctgacc gtcgaggaca tcgaggtcgc
     6181 cccggagcac cgggggcacg gggtcgggcg cgcgttgatg gggctcgcga cggagttcgc
     6241 ccgcgagcgg ggcgccgggc acctctggct ggaggtcacc aacgtcaacg caccggcgat
     6301 ccacgcgtac cggcggatgg ggttcaccct ctgcggcctg gacaccgccc tgtacgacgg
     6361 caccgcctcg gacggcgagc aggcgctcta catgagcatg ccctgcccct aaagatcttc
     6421 agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat
     6481 attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac
     6541 atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg
     6601 tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa
     6661 acgagctcga attcctgggg gaacaacttc acagaatgtt ttgtcatatt gtcgaagtgg
     6721 tcacaaaaca agagaagttc cgccaattat aaaaagggaa cccgtatatt tcagcttcac
     6781 ggatgatttc cagggtgaga gtactgtata tgggcttacg atagaaggcc ataaaaattt
     6841 cttgcttggc aacaaaatag aagtgaaatc atgtcgaggc tgctgtgtgg gagaacagca
     6901 taaaatatca caaaaaaaga atctaaaaca ctgtgttgct tgtcccagaa agggaatcaa
     6961 gtatttttat aaagattgga gtggtaaaaa tcgagtatgt gctagatgct atggaagata
     7021 caaattcagc ggtcatcact gtataaattg caagtatgta ccagaagcac gtgaagtgaa
     7081 aaaggcaaaa gacaaaggcg aaaaattggg cattacgccc gaaggtttgc cagttaaagg
     7141 accagagtgt ataaaatgtg gcggaatctt acagtgcggc cgctctagaa ctagtggatc
     7201 gatccccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
     7261 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     7321 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     7381 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     7441 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     7501 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     7561 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7621 cggggtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7681 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
     7741 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
     7801 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     7861 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     7921 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     7981 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     8041 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     8101 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8161 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8221 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8281 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8341 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8401 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8461 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8521 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8581 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8641 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8701 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8761 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8821 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8881 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8941 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9001 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9061 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9121 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9181 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9241 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9301 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9361 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9421 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9481 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9541 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9601 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9661 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9721 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9781 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9841 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9901 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9961 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10021 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    10081 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    10141 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccgcggctc
    10201 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc t
//
