LOCUS       Exported                9821 bp ds-DNA     circular SYN 08-OCT-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 8, 2019 from SnapGene 5.0.1
            https://www.snapgene.com
COMMENT     Insert from pENTR_Sup35NM 652 to 1442
            Backbone from pSK238_NAT_HO_Sup-mKate2-43-8_pDEST 3196 to 1510
FEATURES             Location/Qualifiers
     source          1..9821
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..9
                     /label=M13-rev
                     /label=M13-rev(1)
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    53..60
                     /label=NotI
                     /note="NotI"
                     /note="color: #804000"
     gene            67..979
                     /label=HO-L taken from 5' upstream region of
                     /note="HO-L taken from 5' upstream region of"
                     /note="HO-L taken from 5' upstream region of HO -1815 to
                     -2721"
                     /note="color: #ffc0cb; direction: RIGHT"
     misc_feature    complement(293..312)
                     /label=Primer 6 on 5' end of HO-L
                     /label=Primer 6 on 5' end of HO-L; used for
                     /note="Primer 6 on 5' end of HO-L; used for"
                     /note="color: #af0850; direction: LEFT"
     protein_bind    995..1003
                     /label=DraIII
                     /note="DraIII"
                     /note="color: #ff0000"
     promoter        1004..1509
                     /label=Sup35 promoter
                     /note="Sup35 promoter"
                     /note="color: #00ffff"
     misc_recomb     1522..1529
                     /label=attR1
                     /note="attR1"
                     /note="color: #ff0000"
     misc_feature    1522..1529
                     /label=attR1
                     /label=attR1(1)
                     /note="attR1"
                     /note="color: #804000"
     misc_recomb     1530..1546
                     /label=attL1
                     /note="attL1"
                     /note="attL1;recognized as attL1"
                     /note="color: #ff0000"
     CDS             1547..2305
                     /codon_start=1
                     /label=Sup35 N, M domains
                     /note="Sup35 N, M domains"
                     /note="color: #ffff00"
                     /translation="MSDSNQGNNQQNYQQYSQNGNQQQGNNRYQGYQAYNAQAQPAGGY
                     YQNYQGYSGYQQGGYQQYNPDAGYQQQYNPQGGYQQYNPQGGYQQQFNPQGGRGNYKNF
                     NYNNNLQGYQAGFQPQSQGMSLNDFQKQQKQAAPKPKKTLKLVSSSGIKLANATKKVGT
                     KPAESDKKEEEKSAETKEPTKEPTKVEEPVKKEEKPVQTEEKTEEKSELPKVEDLKISE
                     STHNTNNANVTSADALIKEQEEEVDDEVVND"
     CDS             1547..1915
                     /codon_start=1
                     /label=N domain
                     /note="N domain"
                     /note="color: #0080ff"
                     /translation="MSDSNQGNNQQNYQQYSQNGNQQQGNNRYQGYQAYNAQAQPAGGY
                     YQNYQGYSGYQQGGYQQYNPDAGYQQQYNPQGGYQQYNPQGGYQQQFNPQGGRGNYKNF
                     NYNNNLQGYQAGFQPQSQG"
     variation       2306
                     /label=C->G
                     /note="color: #00ffff"
     misc_recomb     complement(2307..2316)
                     /label=attL2
                     /note="attL2"
                     /note="attL2 (c);recognized as attL2"
                     /note="color: #ff0000; direction: LEFT"
     variation       2311
                     /label=A->G
                     /note="color: #00ffff"
     misc_recomb     2317..2323
                     /label=attR2
                     /note="attR2"
                     /note="color: #ff0000"
     misc_feature    2324..2362
                     /label=Space between attR1 site
                     /note="Space between attR1 site"
                     /note="color: #804000"
     variation       2327
                     /label=G->T
                     /note="color: #00ffff"
     CDS             2363..2407
                     /label=NLS
                     /note="NLS"
                     /note="color: #ff8080; direction: RIGHT"
     CDS             2420..2653
                     /label=VP16 Activation Domain
                     /note="VP16 Activation Domain"
                     /note="color: #ff8080; direction: RIGHT"
     CDS             2666..2926
                     /label=ZF
                     /note="ZF"
                     /note="color: #0000ff; direction: RIGHT"
     CDS             2942..2971
                     /label=NES
                     /note="NES"
                     /note="color: #ff8080; direction: RIGHT"
     CDS             2993..3184
                     /label=6x HA
                     /note="6x HA"
                     /note="color: #ff8080; direction: RIGHT"
     terminator      3204..3442
                     /label=Sup35 Terminator
                     /note="Sup35 Terminator"
                     /note="color: #b90746"
     misc_signal     3601..3812
                     /label=8x-43-8
                     /note="8x-43-8"
                     /note="color: #ffc0cb"
     misc_feature    3602..3610
                     /label=43-8 ZF operator
                     /note="color: #00ffff"
     misc_feature    3627..3635
                     /label=43-8 ZF operator
                     /label=43-8 ZF operator(1)
                     /note="color: #00ffff"
     misc_feature    3652..3660
                     /label=43-8 ZF operator
                     /label=43-8 ZF operator(2)
                     /note="color: #00ffff"
     misc_feature    3677..3685
                     /label=43-8 ZF operator
                     /label=43-8 ZF operator(3)
                     /note="color: #00ffff"
     misc_feature    3708..3716
                     /label=43-8 ZF operator
                     /label=43-8 ZF operator(4)
                     /note="color: #00ffff"
     misc_feature    3733..3741
                     /label=43-8 ZF operator
                     /label=43-8 ZF operator(5)
                     /note="color: #00ffff"
     misc_feature    3764..3772
                     /label=43-8 ZF operator
                     /label=43-8 ZF operator(6)
                     /note="color: #00ffff"
     misc_feature    3789..3797
                     /label=43-8 ZF operator
                     /label=43-8 ZF operator(7)
                     /note="color: #00ffff"
     promoter        3822..4055
                     /label=Minimal CYC1 promoter
                     /note="Minimal CYC1 promoter"
                     /note="color: #ff80ff"
     CDS             4056..4068
                     /label=ORF1
                     /note="ORF1"
                     /note="color: #ff8080; direction: RIGHT"
     misc_feature    4056..4063
                     /label=PmeI site
                     /note="PmeI site"
                     /note="color: #804000"
     protein_bind    4064..4068
                     /label=RBS
                     /note="RBS"
                     /note="color: #ffff80"
     CDS             4069..4782
                     /label=mKate2
                     /note="color: #ff8080; direction: RIGHT"
     protein_bind    4783..4790
                     /label=AscI
                     /note="AscI"
                     /note="color: #ff0000"
     terminator      4803..4982
                     /label=yADH1_terminator
                     /note="yADH1_terminator"
                     /note="color: #b90746"
     misc_feature    5216..5235
                     /label=kanB
                     /note="kanB"
                     /note="color: #804000"
     CDS             5409..5415
                     /codon_start=1
                     /label=KanR2(variant)
                     /note="KanR2(variant)"
                     /note="ORF frame 3"
                     /note="color: #ff8080"
                     /translation="MG"
     CDS             5416..5975
                     /label=NATr
                     /note="NATr"
                     /note="color: #ff8080; direction: RIGHT"
     misc_feature    complement(6223..6232)
                     /label=R2
                     /note="R2"
                     /note="color: #af0850; direction: LEFT"
     gene            6238..6738
                     /label=HO-R matches 1190 of gene to 1690
                     /note="HO-R matches 1190 of gene to 1690"
                     /note="color: #ffc0cb; direction: RIGHT"
     primer_bind     6624..6643
                     /label=PCR primer 11 for 3' end of HO-R
                     /note="PCR primer 11 for 3' end of HO-R"
                     /note="color: #ffff80; direction: RIGHT"
     misc_feature    6746..6753
                     /label=NotI
                     /label=NotI(1)
                     /note="NotI"
                     /note="color: #ff0000"
     primer_bind     complement(6784..6811)
                     /label=T7
                     /note="color: #00ff00; direction: LEFT"
     primer_bind     complement(6810..6827)
                     /label=M13-fwd
                     /note="color: #00ff00; direction: LEFT"
     CDS             6898..6966
                     /label=LacZ alpha
                     /note="color: #6495ed; direction: RIGHT"
     CDS             8001..8660
                     /label=AmpR
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      8758..9440
                     /label=ColE1 origin
                     /note="color: #808080"
     misc_binding    9782..9804
                     /label=LacO
                     /note="color: #6495ed"
     primer_bind     9810..9821
                     /label=M13-rev
                     /note="color: #00ffff; direction: RIGHT"
ORIGIN
        1 atgaccatga ttacgccact agtccgaggc ctcgagatcc gatatcgccg tggcggccgc
       61 cagctgaagc ttaattatcc tgggcacgag tgaaacaaag ctaaaacctt tatttagcat
      121 ggccattgaa tgtaacaatt atatatatcg caagcacaaa aaatcaagga gagagaacta
      181 ccactttgtt catgtgtaca atgttcatta tctccataag caaaaaaaaa aaaatagaaa
      241 acatatgcta taaggttgat attctcacga gtaagcggca cttgctactt attgacattg
      301 cagatttttg gctacagaaa tagtatatta gagattataa ttgctaatca aatcaaaata
      361 taaaattagt aaaccaaacc atttataccc ttccttagta gttatggatt gttttttaat
      421 gatatttctg caaaccaaag aaagattgtt atccagatag aatttagttt tgatattcat
      481 ttttttgttg aagattgaac gccatatctg ggcctcataa ttcaaaagac ggtgccatta
      541 tcggtagcgt ttcgcattgt actggatttc agaaatttca cagttgatga atcgaaaaga
      601 atggtctcat tgcaacacgt aaggttaaga tgtccctttt taccattata ggcaataaat
      661 gaatcataaa acgaccgtat actggtgaaa tagtagggag aacgagtacc tgtagtaaaa
      721 agtataaatc atagttaatc gggcaatgtc cctcgatcaa ggagtattgt gtcatgttcg
      781 agacaaacgc caacattttt gtttcttttg gacaaatgtt gtttgcattt atgatccgtt
      841 atattttgat ctaatgtaga gttgcacgta gttcttactg gcaaagaaat cgatgcatac
      901 caaaaaagaa taaaggtgat atttgatctt taccgtttag ttccaacgta aaattgtgcc
      961 tttggactta aaatggcgtc gtacgctgca ggtcgacgga tcccaaccac acaaaaatca
     1021 tacaacgaat ggtatatgct tcatttcttt gtttcgcatt agctgcgcta tttgactcaa
     1081 attattattt tttactaaga cgacgcgtca cagtgttcga gtctgtgtca tttcttttgt
     1141 aattctctta aaccacttca taaagttgtg aagttcatag caaaattctt ccgcaaaaag
     1201 atgaatctta gttctcagcc caccaaaaga ggtacatgct aagatcatac agaagttatt
     1261 gtcacttctt accttgctct taaatgtaca ttacaaccgg gtattatatc ttacatcatc
     1321 gtataatatg atctttcttt atggagaaaa tttttttttc actcgaccaa agctcccatt
     1381 gcttctgaag agtgtagtgt atattggtac atcttctctt gaaagactcc attgtactgt
     1441 aacaaaaagc ggtttcttca tcgacttgct cggaataaca tctatatctg cccactagca
     1501 acaactagtg gatcccccat cacaagtttg tacaaaaaag caggctatgt cggattcaaa
     1561 ccaaggcaac aatcagcaaa actaccagca atacagccag aacggtaacc aacaacaagg
     1621 taacaacaga taccaaggtt atcaagctta caatgctcaa gcccaacctg caggtgggta
     1681 ctaccaaaat taccaaggtt attctgggta ccaacaaggt ggctatcaac agtacaatcc
     1741 cgacgccggt taccagcaac agtataatcc tcaaggaggc tatcaacagt acaatcctca
     1801 aggcggttat cagcagcaat tcaatccaca aggtggccgt ggaaattaca aaaacttcaa
     1861 ctacaataac aatttgcaag gatatcaagc tggtttccaa ccacagtctc aaggtatgtc
     1921 tttgaacgac tttcaaaagc aacaaaagca ggccgctccc aaaccaaaga agactttgaa
     1981 gcttgtctcc agttccggta tcaagttggc caatgctacc aagaaggttg gcacaaaacc
     2041 tgccgaatct gataagaaag aggaagagaa gtctgctgaa accaaagaac caactaaaga
     2101 gccaacaaag gtcgaagaac cagttaaaaa ggaggagaaa ccagtccaga ctgaagaaaa
     2161 gacggaggaa aaatcggaac ttccaaaggt agaagacctt aaaatctctg aatcaacaca
     2221 taataccaac aatgccaatg ttaccagtgc tgatgccttg atcaaggaac aggaagaaga
     2281 agtggatgac gaagttgtta acgatgaccc ggctttcttg tacaaagtgg tgatgggctg
     2341 caggaattcg atatcaagct taatggctcc aaagaagaag agaaaggttg gtattcatgg
     2401 tgttccagtt gatggtggtt tacaagctcc accaactgat gtttctttgg gtgatgaatt
     2461 gcatttggat ggtgaagatg ttgctatggc tcatgctgat gctttggatg attttgattt
     2521 ggatatgttg ggtgacggtg attctccagg tccaggtttt actccacatg attctgctcc
     2581 atatggtgct ttagatatgg ctgactttga attcgaacaa atgttcaccg atgccttggg
     2641 tattgatgaa tatggtggtg gtgctagacc aggtgaaaga ccatttcaat gtagaatctg
     2701 catgagaaac ttctccagac aagatagatt ggatagacat accagaactc atactggtga
     2761 aaagccattc caatgcagaa tatgtatgag aaatttttcc caaaaagaac acttggccgg
     2821 tcatttgaga acacacacag gtgaaaaacc ttttcaatgc agaatatgca tgagaaactt
     2881 tagtagaaga gacaacttga acagacactt gaaaacccat ttgagaggtt ctgccggcga
     2941 attggctttg aaattggctg gtttggatat caattctggt tctgctgcta gatacccata
     3001 tgatgttcca gattatgctg gttaccctta cgatgttcct gactacgcag gttcttatcc
     3061 atacgatgta cctgattatg cctcaagata tccttatgac gtccctgatt acgccggtta
     3121 tccatatgac gtaccagact acgctggtag ttacccatac gacgtcccag attacgcttc
     3181 ttcataatga ctcgagtcat gtaatttctt gcaaacataa gtaaatgcaa acacaataat
     3241 accgatcata aagcattttc ttctatatta aaaaacaagg tttaataaag ctgttatata
     3301 tatatatata tatatagacg tataattagt ttagttcttt ttgtaccata taccataaac
     3361 aaggtaaact tcacctctca atatatctag aatttcataa aaatatctag caaggtttca
     3421 actccttcaa tcacgttttc atcactcaac cctatctcgg tctattcttt tgatttataa
     3481 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3541 gcgaatttta acaaaatatt aacgtttaca atttcctgat gcggtatttt ctttaattaa
     3601 agagtgagga ctcgaaaata ttaatagagt gaggactcga aaatattaat agagtgagga
     3661 ctcgaaaata ttaatagagt gaggactcga aaatattaat atcgatagag tgaggactcg
     3721 aaaatattaa tagagtgagg actcgaaaat attaatgaat tcagagtgag gactcgaaaa
     3781 tattaataga gtgaggactc gaaaatatta atggccggcc cagatccgcc aggcgtgtat
     3841 atatagcgtg gatggccagg caactttagt gctgacacat acaggcatat atatatgtgt
     3901 gcgacgacac atgatcatat ggcatgcatg tgctctgtat gtatataaaa ctcttgtttt
     3961 cttcttttct ctaaatattc tttccttata cattaggacc tttgcagcat aaattactat
     4021 acttctatag acacacaaac acaaatacac acactgttta aacaaaaaat gggttctggt
     4081 ggttctgaat tgatcaaaga aaacatgcac atgaagttgt acatggaagg tactgttaac
     4141 aaccatcatt tcaagtgtac ctctgaaggt gaaggtaaac catatgaagg tactcaaacc
     4201 atgagaatca aagctgttga aggtggtcca ttgccatttg cttttgatat tttggctacc
     4261 tccttcatgt acggttctaa gactttcatt aaccacaccc aaggtatccc agatttcttc
     4321 aaacaatctt tcccagaagg tttcacttgg gaaagagtta ctacttacga agatggtggt
     4381 gttttgactg ctactcaaga tacttcttta caagacggtt gcttgatcta caacgttaag
     4441 attagaggtg tcaacttccc atctaatggt ccagttatgc aaaaaaagac tttgggttgg
     4501 gaagcttcta ctgaaacatt atatccagct gatggtggtt tggaaggtag agctgatatg
     4561 gctttgaaat tggttggtgg tggtcatttg atctgcaact tgaaaactac ctacagatct
     4621 aagaagccag ccaagaattt gaaaatgcca ggtgtttact acgtcgatag aagattggaa
     4681 agaatcaaag aagccgacaa agaaacctac gttgaacaac acgaagttgc tgttgctaga
     4741 tattgtgatt tgccatccaa attaggtcat agaggatctt aaggcgcgcc acttctaaat
     4801 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     4861 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     4921 ctttcctgta ggtcaggttg ctttctcagg tatagtatga ggtcgctctt attgaccaca
     4981 cctctaccgg cagatccgct agggataaca gggtaatata gatctgttta gcttgcctcg
     5041 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
     5101 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
     5161 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
     5221 ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
     5281 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
     5341 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
     5401 aaacaaccat gggtaccact cttgacgaca cggcttaccg gtaccgcacc agtgtcccgg
     5461 gggacgccga ggccatcgag gcactggatg ggtccttcac caccgacacc gtcttccgcg
     5521 tcaccgccac cggggacggc ttcaccctgc gggaggtgcc ggtggacccg cccctgacca
     5581 aggtgttccc cgacgacgaa tcggacgacg aatcggacga cggggaggac ggcgacccgg
     5641 actcccggac gttcgtcgcg tacggggacg acggcgacct ggcgggcttc gtggtcgtct
     5701 cgtactccgg ctggaaccgc cggctgaccg tcgaggacat cgaggtcgcc ccggagcacc
     5761 gggggcacgg ggtcgggcgc gcgttgatgg ggctcgcgac ggagttcgcc cgcgagcggg
     5821 gcgccgggca cctctggctg gaggtcacca acgtcaacgc accggcgatc cacgcgtacc
     5881 ggcggatggg gttcaccctc tgcggcctgg acaccgccct gtacgacggc accgcctcgg
     5941 acggcgagca ggcgctctac atgagcatgc cctgccccta atcagtactg acaataaaaa
     6001 gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt
     6061 ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc
     6121 gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat
     6181 actgctgtcg attcgatact aacgccgcca tccagtgtcg aaaacgagct cgaattcctg
     6241 ggggaacaac ttcacagaat gttttgtcat attgtcgaag tggtcacaaa acaagagaag
     6301 ttccgccaat tataaaaagg gaacccgtat atttcagctt cacggatgat ttccagggtg
     6361 agagtactgt atatgggctt acgatagaag gccataaaaa tttcttgctt ggcaacaaaa
     6421 tagaagtgaa atcatgtcga ggctgctgtg tgggagaaca gcataaaata tcacaaaaaa
     6481 agaatctaaa acactgtgtt gcttgtccca gaaagggaat caagtatttt tataaagatt
     6541 ggagtggtaa aaatcgagta tgtgctagat gctatggaag atacaaattc agcggtcatc
     6601 actgtataaa ttgcaagtat gtaccagaag cacgtgaagt gaaaaaggca aaagacaaag
     6661 gcgaaaaatt gggcattacg cccgaaggtt tgccagttaa aggaccagag tgtataaaat
     6721 gtggcggaat cttacagtgg cctatgcggc cgctctagaa ctagtggatc gatccccaat
     6781 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
     6841 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     6901 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6961 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     7021 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7081 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     7141 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggtgggc
     7201 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7261 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
     7321 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
     7381 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     7441 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     7501 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7561 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     7621 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     7681 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7741 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7801 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7861 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7921 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7981 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8041 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8101 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8161 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8221 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8281 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8341 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8401 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8461 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8521 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8581 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8641 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8701 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8761 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8821 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8881 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8941 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     9001 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9061 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9121 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9181 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9241 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9301 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9361 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9421 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9481 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9541 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9601 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9661 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     9721 agctcactca ttaggcaccc caggctttac actttatgct tccgcggctc gtatgttgtg
     9781 tggaattgtg agcggataac aatttcacac aggaaacagc t
//
