LOCUS       Exported                7223 bp ds-DNA     circular UNA 29-OCT-2019
DEFINITION  natural circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 29, 2019 from SnapGene 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     CDS             1207..2205
                     /codon_start=1
                     /label=repB
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="/vntifkey=4"
                     /translation="MLQNDVFIDFTNKINSIRDCNKYWYLDVYKKQKIKDFKKTNLCKD
                     KFCNNCKKVKQASRMQKYIPELQKYKDGLYHFIFTVENVPGSELRDTIDRLFKSFKSFT
                     RYLSGNLKIKGVNFDKWGYKGCVRSLEVTYSMIDNHIMYHPHLHVAMILDPFYDGFNVE
                     RMHIINKFSYSYGVLKRLFTDDELLIQKIWYLLFNNIEVNMANINNLEDGYSCLVNKFS
                     DYDYAELFKYICKNTDEQGLLMTYDIFKDLYFALHNVHQIQGYGCLYNIRDDTQLDLKV
                     DDIYNDLIDLLQVTENPIQSMETVQDLLKDTEYTIISRKRIFKYLTQLYHKD"
     CDS             complement(2401..3884)
                     /codon_start=1
                     /label=Kan
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="/vntifkey=4"
                     /translation="KPSEPFEVIGKIIPRYENENWTFTELLYEAPYLKSYQDEEDEEDE
                     EADCLEYIDNTDKIIYLLYRRYRRM*GFQGARHRQRAYQYIYRMGKA*KLAWTNA*NPG
                     Q*PYSL*ILS*LWFQNRLRRYYVIRQL*KQL*KSCFLVFKVLECKEQ*IGVRLVIISFL
                     GYL*IL*KRGRK**MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF*HLDLDV*KALTTSFLD
                     I*SFLKYIRNCPYSDVLYLF*KFAR*GSRAPTRNLY"
     promoter        3885..4505
                     /label=C. therm CBP prom
                     /note="/ApEinfo_fwdcolor=#346ee0"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=#346ee0"
                     /note="/vntifkey=30"
     primer_bind     complement(4483..4505)
                     /label=pMU876_rev
                     /label=nonstandard type:  Primer
     terminator      4506..4682
                     /label=T1+T2 term
                     /note="/ApEinfo_fwdcolor=#9d1b1c"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=#9d1b1c"
                     /note="/vntifkey=43"
     primer_bind     4506..4527
                     /label=pMU876_fwd
                     /label=nonstandard type:  Primer
     promoter        complement(4742)
                     /label=P(LAC)
                     /note="lac promoter"
                     /note="/ApEinfo_fwdcolor=#346ee0"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=#346ee0"
                     /note="/vntifkey=30"
     misc_feature    5008
                     /label=ORI
                     /label=nonstandard type:  Replication_ori
                     /note="RNaseH cleavage point"
                     /note="/ApEinfo_fwdcolor=#999999"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=#999999"
                     /note="/vntifkey=33"
     CDS             complement(5770..6627)
                     /codon_start=1
                     /label=AP(R)
                     /note="bla gene- Ap(r) determinant"
                     /note="/ApEinfo_fwdcolor=#e9d024"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=#e9d024"
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6662)
                     /label=P(BLA)
                     /note="bla gene promoter"
                     /note="/ApEinfo_fwdcolor=#346ee0"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=#346ee0"
                     /note="/vntifkey=30"
ORIGIN
        1 aattgacaaa gttttctatt tgtgttaaca ttgtttatat aatagtgaac agtgttaaga
       61 ttaaatgtga ggtgtttgta tggatattaa tgattataaa gagaagggac tttatttatt
      121 aagtagtatg gatgatttta ttaaaattaa tgatttgttt atgggtaaag ttgtttctcc
      181 tggctatgtt gcttcggttt ttggtgtttc caggtctact gttacacaat ggattcaaag
      241 acgtaaaatt agagctttta agtataaagg taaggaaggt gactatatgg ttatacctat
      301 tgctgatatt attgattaca aaagattgag taataatgat tttatttatg ataagttagt
      361 gaggtgattt attttatgtt tgacgatagc tatgttgtta atgagtgttc gtctaatgtt
      421 agtgaaaatg atagagattt ttgtagtttg gttggtcgtt ttatgattat taatggtata
      481 gataagttgg ttattaagat taatagaaaa tttaatagga aatctttaag tttagatttt
      541 agtgttgatt tattcccttc tatcaaagtt tctgaattag ttttttttga tgagtttaac
      601 aaaacgtgtg gtttttattt ttcttttaat tcttttacaa tttttaaggc ttttagagat
      661 gttcataatc ataataaaat atcattttat tttgcataat ttcgggtctg ggccgcagac
      721 caggcccagt gctaacaata ttaattttta atgttaggaa ttgtttaatt cttaattgtg
      781 tttttaaagg tagaataatt acccattcgc cctttagcca acaaaaatta aggaggtata
      841 aacatggata aaatggattt gattcttcaa gatgaaagac tgggtgagat atttaaagat
      901 atagatttaa cagataatga aaagagatat cttaaatggt tatggaaatg ggattatgaa
      961 acacgtgata cttttgtatc aatttttttg aagctaaaaa atggtggaaa atgatttttt
     1021 tcttatcttg atatattaga aaaaagcgta ctcacgaagt aagaatttgt aaaaaaagaa
     1081 ggggggattt ttttggatga gagtttgtac aagcagattt taagtaatat tattattact
     1141 cgtgattatt gtaaaaatgt tttagataat ataaagttca atgaaaaaat aattgattat
     1201 tatgttatgt tacaaaatga tgtttttatt gattttacta ataaaataaa ttcaataagg
     1261 gattgtaata aatattggta tttggatgtt tataaaaagc agaaaataaa ggattttaaa
     1321 aagactaatt tgtgtaaaga taagttctgt aataattgta agaaagttaa acaggcttca
     1381 agaatgcaaa aatatattcc tgaattacag aaatacaaag atggcttata tcattttata
     1441 tttactgttg aaaatgtgcc aggtagtgaa ttaagagata ctattgatag gttgtttaag
     1501 tcttttaagt catttacaag gtatttaagt ggtaatctta aaataaaagg tgttaatttt
     1561 gataaatggg gttataaagg ctgtgtaagg tctttagagg taacttatag tatgattgat
     1621 aatcatatta tgtatcatcc acacttgcat gttgcgatga tattagatcc tttttacgat
     1681 ggttttaatg ttgaaaggat gcatataatt aataagttta gttatagcta tggtgtttta
     1741 aaaaggttgt ttactgatga tgaattatta attcaaaaaa tttggtattt attgtttaat
     1801 aatattgagg ttaacatggc caatataaat aatttagagg atggttattc ttgtttagtt
     1861 aataagttta gtgattatga ttatgcggag ctgtttaagt atatttgtaa aaatactgat
     1921 gaacaaggtt tacttatgac ttatgatatt tttaaagatt tatattttgc attacataat
     1981 gttcatcaga tacaaggcta tggttgttta tataatataa gagatgatac tcaattagat
     2041 ttaaaggttg atgacattta taatgatttg attgatttat tacaagttac agaaaatcct
     2101 atacagtcta tggaaactgt acaggattta ttaaaggata ctgaatatac aataataagc
     2161 cgtaagcgta tatttaagta tctaacacaa ttatatcata aggattgata tttataccgt
     2221 ctgtcggact catgcggagg gggacttgag ggggtctccc ctcgcattgt acgacagacg
     2281 gtattattat tatacaaatt ttttttatgt aatttttttt gtgtaatttt tttatacaaa
     2341 taatatttca attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca
     2401 cgatacaaat tcctcgtagg cgctcgggac ccctatctag cgaactttta gaaaagatat
     2461 aaaacatcag agtatggaca gttgcggatg tacttcagaa aagattagat gtctaaaaag
     2521 cttgtagtta aagcttttta gacatctaaa tctaggtgct aaaacaattc atccagtaaa
     2581 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     2641 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
     2701 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
     2761 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
     2821 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
     2881 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
     2941 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
     3001 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
     3061 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
     3121 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
     3181 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
     3241 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
     3301 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
     3361 ttccttcccc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
     3421 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
     3481 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccacaat
     3541 tatgatagaa tttacaagct ataaggttat tgtcctgggt ttcaagcatt agtccatgca
     3601 agtttttatg ctttgcccat tctatagata tattgataag cgcgctgcct atgccttgcc
     3661 ccctgaaatc cttacatacg gcgatatctt ctatataaaa gatatattat cttatcagta
     3721 ttgtcaatat attcaaggca atctgcctcc tcatcctctt catcctcttc gtcttggtag
     3781 ctttttaaat atggcgcttc atagagtaat tctgtaaagg tccaattctc gttttcatac
     3841 ctcggtataa tcttacctat cacctcaaat ggttcgctgg gtttgagtcg tgactaagaa
     3901 cgtcaaagta attaacaata cagctatttt tctcatgctt ttaccccttt cataaaattt
     3961 aattttatcg ttatcataaa aaattataga cgttatattg cttgccggga tatagtgctg
     4021 ggcattcgtt ggtgcaaaat gttcggagta aggtggatat tgatttgcat attgatctat
     4081 tgcattgaaa tgattagtta tccgtaaata ttaattaatc atatcataaa ttaattatat
     4141 cataattgtt ttgacgaatg aaggtttttg gataaattat caagtaaagg aacgctaaaa
     4201 attttggcgt aaaatatcaa aatgaccact tgaattaata tggtaaagta gatataatat
     4261 tttggtaaac atgccttcag caaggttaga ttagctgttt ccgtataaat taaccgtatg
     4321 gtaaaacggc agtcagaaaa ataagtcata agattccgtt atgaaaatat acttcggtag
     4381 ttaataataa gagatatgag gtgagagata caagataaga gatataaggt acgaatgtat
     4441 aagatggtgc ttttaggcac actaaataaa aaacaaataa acgaaaattt taaggaggac
     4501 gaaagctggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     4561 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
     4621 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt
     4681 ttagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     4741 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     4801 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     4861 gccagcagat ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4921 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4981 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5041 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5101 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5161 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5221 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5281 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5341 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5401 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5461 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5521 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5581 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5641 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5701 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5761 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5821 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5881 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     5941 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6001 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6061 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6121 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6181 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6241 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6301 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6361 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6421 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6481 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6541 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6601 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6661 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6721 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     6781 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     6841 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     6901 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     6961 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     7021 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     7081 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     7141 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     7201 acgttgtaaa acgacggcca gtg
//
