LOCUS       pDONR223_ATG3_C264A    3738 bp      DNA     circular   28/06/2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=GateWay(tm) BP reaction between PCR ATG3wt attB(addgene-8295
            2-pDONR-ATG3) as an Expression fragment and pDONR223 as a Do
            nor vector.
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     terminator      complement(270..297)
                     /label=rrnB T2 terminator
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="rrnB_T2_terminator"complement(268..295) \ 
     terminator      complement(429..472)
                     /label=rrnB T1 terminator
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="rrnB_T1_terminator" - complement(427..470) \ 
     misc_feature    524..546
                     /label=M13 pUC fwd primer
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_pUC_fwd_primer" 522..544 \ 
     promoter        539..555
                     /label=M13 forward20 primer
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_forward20_primer" 537..553 \ 
     misc_feature    complement(571..654)
                     /label=attL1
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="attL1"complement(569..652) \ 
     misc_feature    1631..1718
                     /label=attL2
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="attL2" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCD \ 
     promoter        complement(1736..1754)
                     /label=T7 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="T7_promoter" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNE \ 
     promoter        complement(1755..1773)
                     /label=M13 reverse primer
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_reverse_primer" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFH \ 
     gene            1987..2997
                     /label=spect
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="spect" - gene="spect" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCD \ 
     rep_origin      3074..3693
                     /label=pBR322_origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="pBR322_origin" - translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA - RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL - AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE - VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLA \ 
                     653..1461
                     /label=Insert from PCR ATG3wt attB(addgene-82952-pDONR-ATG3) [*]
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment inserted from PCR ATG3wt attB(addgene-82952-pDONR-ATG3) into pDONR223 [BP] reaction \ 
                     1462..1462
                     /label=Insert from PCR ATG3wt attB(addgene-82952-pDONR-ATG3) [*] [5']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment inserted from PCR ATG3wt attB(addgene-82952-pDONR-ATG3) into pDONR223 [BP] reaction \ 
                     1465..1634
                     /label=Insert from PCR ATG3wt attB(addgene-82952-pDONR-ATG3) [*] [3']
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment inserted from PCR ATG3wt attB(addgene-82952-pDONR-ATG3) into pDONR223 [BP] reaction \ 
     CDS             673..1461
                     /label=ATG3 CDS [*]
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEEDDGDGGWVDTYHNTGITGITEAVKEITLENKDNIRLQDCSALCEEEEDEDEGEAADMEEYEESGLLETDEATLDTRKIVEACKAKTDAGGEDAILQTRTYDLYITYDKY \ 
     CDS             1462..1462
                     /label=ATG3 CDS [*] [5']
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEEDDGDGGWVDTYHNTGITGITEAVKEITLENKDNIRLQDCSALCEEEEDEDEGEAADMEEYEESGLLETDEATLDTRKIVEACKAKTDAGGEDAILQTRTYDLYITYDKY \ 
     CDS             1465..1614
                     /label=ATG3 CDS [*] [3']
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEEDDGDGGWVDTYHNTGITGITEAVKEITLENKDNIRLQDCSALCEEEEDEDEGEAADMEEYEESGLLETDEATLDTRKIVEACKAKTDAGGEDAILQTRTYDLYITYDKY \ 
ORIGIN
        1 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT 
       61 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA 
      121 GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG 
      181 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TACGCGTACC 
      241 GCTAGCCAGG AAGAGTTTGT AGAAACGCAA AAAGGCCATC CGTCAGGATG GCCTTCTGCT 
      301 TAGTTTGATG CCTGGCAGTT TATGGCGGGC GTCCTGCCCG CCACCCTCCG GGCCGTTGCT 
      361 TCACAACGTT CAAATCCGCT CCCGGCGGAT TTGTCCTACT CAGGAGAGCG TTCACCGACA 
      421 AACAACAGAT AAAACGAAAG GCCCAGTCTT CCGACTGAGC CTTTCGTTTT ATTTGATGCC 
      481 TGGCAGTTCC CTACTCTCGC GTTAACGCTA GCATGGATGT TTTCCCAGTC ACGACGTTGT 
      541 AAAACGACGG CCAGTCTTAA GCTCGGGCCC CAAATAATGA TTTTATTTTG ACTGATAGTG 
      601 ACCTGTTCGT TGCAACAAAT TGATGAGCAA TGCTTTTTTA TAATGCCAAC TTTGTACAAA 
      661 AAAGCAGGCT TAATGCAGAA TGTGATTAAT ACTGTGAAGG GAAAGGCACT GGAAGTGGCT 
      721 GAGTACCTGA CCCCGGTCCT CAAGGAATCA AAGTTTAAGG AAACAGGTGT AATTACCCCA 
      781 GAAGAGTTTG TGGCAGCTGG AGATCACCTA GTCCACCACT GTCCAACATG GCAATGGGCT 
      841 ACAGGGGAAG AATTGAAAGT GAAGGCATAC CTACCAACAG GCAAACAATT TTTGGTAACC 
      901 AAAAATGTGC CGTGCTATAA GCGGTGCAAA CAGATGGAAT ATTCAGATGA ATTGGAAGCT 
      961 ATCATTGAAG AAGATGATGG TGATGGCGGA TGGGTAGATA CATATCACAA CACAGGTATT 
     1021 ACAGGAATAA CGGAAGCCGT TAAAGAGATC ACACTGGAAA ATAAGGACAA TATAAGGCTT 
     1081 CAAGATTGCT CAGCACTATG TGAAGAGGAA GAAGATGAAG ATGAAGGAGA AGCTGCAGAT 
     1141 ATGGAAGAAT ATGAAGAGAG TGGATTGTTG GAAACAGATG AGGCTACCCT AGATACAAGG 
     1201 AAAATAGTAG AAGCTTGTAA AGCCAAAACT GATGCTGGCG GTGAAGATGC TATTTTGCAA 
     1261 ACCAGAACTT ATGACCTTTA CATCACTTAT GATAAATATT ACCAGACTCC ACGATTATGG 
     1321 TTGTTTGGCT ATGATGAGCA ACGGCAGCCT TTAACAGTTG AGCACATGTA TGAAGACATC 
     1381 AGTCAGGATC ATGTGAAGAA AACAGTGACC ATTGAAAATC ACCCTCATCT GCCACCACCT 
     1441 CCCATGTGTT CAGTTCACCC AGCCAGGCAT GCTGAGGTGA TGAAGAAAAT CATTGAGACT 
     1501 GTTGCAGAAG GAGGGGGAGA ACTTGGAGTT CATATGTATC TTCTTATTTT CTTGAAATTT 
     1561 GTACAAGCTG TCATTCCAAC AATAGAATAT GACTACACAA GACACTTCAC AATGTAATAC 
     1621 CCAGCTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC 
     1681 GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT 
     1741 AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT 
     1801 CTCTGATGTT ACATTGCACA AGATAAAAAT ATATCATCAT GCCTCCTCTA GACCAGCCAG 
     1861 GACAGAAATG CCTCGACTTC GCTGCTGCCC AAGGTTGCCG GGTGACGCAC ACCGTGGAAA 
     1921 CGGATGAAGG CACGAACCCA GTGGACATAA GCCTGTTCGG TTCGTAAGCT GTAATGCAAG 
     1981 TAGCGTATGC GCTCACGCAA CTGGTCCAGA ACCTTGACCG AACGCAGCGG TGGTAACGGC 
     2041 GCAGTGGCGG TTTTCATGGC TTGTTATGAC TGTTTTTTTG GGGTACAGTC TATGCCTCGG 
     2101 GCATCCAAGC AGCAAGCGCG TTACGCCGTG GGTCGATGTT TGATGTTATG GAGCAGCAAC 
     2161 GATGTTACGC AGCAGGGCAG TCGCCCTAAA ACAAAGTTAA ACATCATGAG GGAAGCGGTG 
     2221 ATCGCCGAAG TATCGACTCA ACTATCAGAG GTAGTTGGCG TCATCGAGCG CCATCTCGAA 
     2281 CCGACGTTGC TGGCCGTACA TTTGTACGGC TCCGCAGTGG ATGGCGGCCT GAAGCCACAC 
     2341 AGTGATATTG ATTTGCTGGT TACGGTGACC GTAAGGCTTG ATGAAACAAC GCGGCGAGCT 
     2401 TTGATCAACG ACCTTTTGGA AACTTCGGCT TCCCCTGGAG AGAGCGAGAT TCTCCGCGCT 
     2461 GTAGAAGTCA CCATTGTTGT GCACGACGAC ATCATTCCGT GGCGTTATCC AGCTAAGCGC 
     2521 GAACTGCAAT TTGGAGAATG GCAGCGCAAT GACATTCTTG CAGGTATCTT CGAGCCAGCC 
     2581 ACGATCGACA TTGATCTGGC TATCTTGCTG ACAAAAGCAA GAGAACATAG CGTTGCCTTG 
     2641 GTAGGTCCAG CGGCGGAGGA ACTCTTTGAT CCGGTTCCTG AACAGGATCT ATTTGAGGCG 
     2701 CTAAATGAAA CCTTAACGCT ATGGAACTCG CCGCCCGACT GGGCTGGCGA TGAGCGAAAT 
     2761 GTAGTGCTTA CGTTGTCCCG CATTTGGTAC AGCGCAGTAA CCGGCAAAAT CGCGCCGAAG 
     2821 GATGTCGCTG CCGACTGGGC AATGGAGCGC CTGCCGGCCC AGTATCAGCC CGTCATACTT 
     2881 GAAGCTAGAC AGGCTTATCT TGGACAAGAA GAAGATCGCT TGGCCTCGCG CGCAGATCAG 
     2941 TTGGAAGAAT TTGTCCACTA CGTGAAAGGC GAGATCACCA AGGTAGTCGG CAAATAACCC 
     3001 TCGAGCCACC CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA 
     3061 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC 
     3121 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA 
     3181 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT 
     3241 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC 
     3301 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG 
     3361 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 
     3421 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG 
     3481 CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC 
     3541 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT 
     3601 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG 
     3661 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 
     3721 TGGCCTTTTG CTCACATG
//