LOCUS       pCMV_3xFLAG-ATG3_1-13KR    5693 bp      DNA     circular   28/06/2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : CMV N-3xFLAG-ATG3 WT [5124  nt] : (PstI[3734] 
            / KpnI[3169]) to CMV N-3xFLAG-ATG3 allKR [561  nt] : (KpnI[3
            169] / PstI[3734])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     2380..2397
                     /label=M13-fwd
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13-fwd - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - 141..158 \ 
     rep_origin      complement(3..685)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=ColE1 origin - ApEinfo_fwdcolor=gray50 - ApEinfo_revcolor=gray50 - complement(4164..4846) \ 
     rep_origin      1789..2095
                     /label=F1 ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=F1 ori - ApEinfo_fwdcolor=gray50 - ApEinfo_revcolor=gray50 - 5950..6256 \ 
     CDS             complement(2241..2309)
                     /label=LacZ alpha
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=LacZ alpha - ApEinfo_fwdcolor=#6495ed - ApEinfo_revcolor=#6495ed - complement(2..70) \ 
     CDS             complement(783..1442)
                     /label=AmpR
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=AmpR - ApEinfo_fwdcolor=yellow - ApEinfo_revcolor=yellow - complement(4944..5603) \ 
     promoter        2634..3160
                     /label=CMV promoter
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CMV promoter - ApEinfo_fwdcolor=cornflower blue - ApEinfo_revcolor=cornflower blue - 395..921 \ 
     misc_feature    4063..4065
                     /label=C264
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="C" \ 
                     3174..3734
                     /label=insert from CMV N-3xFLAG-ATG3 allKR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment extracted from CMV N-3xFLAG-ATG3 allKR [561  nt] : (KpnI[3169] / PstI[3734]) \ 
     primer_bind     complement(5309..5329)
                     /label=M13-rev
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13-rev - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - complement(3777..3797) \ 
     primer_bind     complement(5279..5298)
                     /label=T7
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=T7 - ApEinfo_fwdcolor=cyan - ApEinfo_revcolor=green - complement(3747..3766) \ 
     misc_binding    complement(5335..5357)
                     /label=LacO
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=LacO - ApEinfo_fwdcolor=#6495ed - ApEinfo_revcolor=#6495ed - complement(3803..3825) \ 
     promoter        5045..5241
                     /label=SV40 early promoter
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 early promoter - ApEinfo_fwdcolor=cornflower blue - ApEinfo_revcolor=cornflower blue - 3513..3709 \ 
     polyA_signal    4261..4741
                     /label=hGH polyA signal
                     /SerialCloner_Color=&hFFC0CB
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=hGH polyA signal - ApEinfo_fwdcolor=pink - ApEinfo_revcolor=pink - 2729..3209 \ 
     misc_feature    4246..4248
                     /label=Stop-codon
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Stop-codon - ApEinfo_fwdcolor=#00ff00 - ApEinfo_revcolor=green - 2714..2716 \ 
     misc_feature    3175..3243
                     /label=3xflag
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=3xflag - ApEinfo_fwdcolor=#00ff00 - ApEinfo_revcolor=green - 1002..1004 \ 
     promoter        complement(5280..5298)
                     /label=T7 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="T7_promoter" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNE \ 
     promoter        complement(5309..5327)
                     /label=M13 reverse primer
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_reverse_primer" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFH \ 
ORIGIN
        1 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT 
       61 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 
      121 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC 
      181 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT 
      241 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC 
      301 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG 
      361 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 
      421 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC 
      481 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 
      541 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA 
      601 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 
      661 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 
      721 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT 
      781 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA 
      841 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC 
      901 AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC 
      961 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG 
     1021 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC 
     1081 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC 
     1141 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG 
     1201 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC 
     1261 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT 
     1321 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC 
     1381 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC 
     1441 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC 
     1501 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC 
     1561 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA 
     1621 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     1681 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     1741 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     1801 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     1861 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     1921 AATCGGGGCA TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     1981 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     2041 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     2101 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     2161 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACAAAA 
     2221 TATTAACGTT TACAATTTCC CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA 
     2281 TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA 
     2341 TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC 
     2401 AAGCTGATCT ATACATTGAA TCAATATTGG CAATTAGCCA TATTAGTCAT TGGTTATATA 
     2461 GCATAAATCA ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 
     2521 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGAC ATTGATTATT GACTAGTTAT 
     2581 TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA 
     2641 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA 
     2701 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG 
     2761 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 
     2821 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC 
     2881 TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG 
     2941 ATGCGGTTTT GGCAGTACAC CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA 
     3001 AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT 
     3061 CCAAAATGTC GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 
     3121 GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA ATTGATCTGg tacCATGGAC 
     3181 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ATTACAAGGA TGACGATGAC 
     3241 AAGCTTACAA GTTTGTACAA AAAAGCAGGC TTAATGCAGA ATGTGATTAA TACTGTGAGG 
     3301 ggaAGGgcac tggaagtggc tgagtacctg accccggtcc tcAGGgaatc aAGGtttAGG 
     3361 gaaacaggtg taattacccc agaagagttt gtggcagctg gagatcacct agtccaccac 
     3421 tgtccaacat ggcaatgggc tacaggggaa gaattgAGAg tgAGGgcata cctaccaaca 
     3481 ggcAGAcaat ttttggtaac cAGAaatgtg ccgtgctatA GGcggtgcAG Acagatggaa 
     3541 tattcagatg aattggaagc tatcattgaa gaagatgatg gtgatggcgg atgggtagat 
     3601 acatatcaca acacaggtat tacaggaata acggaagccg ttAGAgagat cacactggaa 
     3661 aatAGGgaca atataaggct tcaagattgc tcagcactat gtgaagagga agaagatgaa 
     3721 gatgaaggag aagctgcaGA TATGGAAGAA TATGAAGAGA GTGGATTGTT GGAAACAGAT 
     3781 GAGGCTACCC TAGATACAAG GAAAATAGTA GAAGCTTGTA AAGCCAAAAC TGATGCTGGC 
     3841 GGTGAAGATG CTATTTTGCA AACCAGAACT TATGACCTTT ACATCACTTA TGATAAATAT 
     3901 TACCAGACTC CACGATTATG GTTGTTTGGC TATGATGAGC AACGGCAGCC TTTAACAGTT 
     3961 GAGCACATGT ATGAAGACAT CAGTCAGGAT CATGTGAAGA AAACAGTGAC CATTGAAAAT 
     4021 CACCCTCATC TGCCACCACC TCCCATGTGT TCAGTTCACC CATGCAGGCA TGCTGAGGTG 
     4081 ATGAAGAAAA TCATTGAGAC TGTTGCAGAA GGAGGGGGAG AACTTGGAGT TCATATGTAT 
     4141 CTTCTTATTT TCTTGAAATT TGTACAAGCT GTCATTCCAA CAATAGAATA TGACTACACA 
     4201 AGACACTTCA CAATGTAATA CCCAGCTTTC TTGTACAAAG TGGTGTGATC TAGAGGATCC 
     4261 CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC 
     4321 AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC 
     4381 CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC 
     4441 AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG 
     4501 GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT 
     4561 GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG 
     4621 GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC 
     4681 TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT 
     4741 GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGCCAT 
     4801 GGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC 
     4861 CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TCTGTGGAAT GTGTGTCAGT 
     4921 TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 
     4981 ATTAGTCAGC AACCAGGTGT GGAAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5041 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     5101 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     5161 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     5221 GGAGGCCTAG GCTTTTGCAA AAAGCTCCTC GAGGAACTGA AAAACCAGAA AGTTAATTCC 
     5281 CTATAGTGAG TCGTATTAAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT 
     5341 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT 
     5401 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG 
     5461 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG 
     5521 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG 
     5581 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT 
     5641 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAA
//