LOCUS       Gaolf_Integration_Vecto 8115 bp ds-DNA     circular     10-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     protein_bind    1628..1646
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    1406..1424
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     repeat_unit     complement(887..917)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     repeat_unit     complement(874..886)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1443..1461
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3964..4560
                     /label="puro"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1665..1683
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4570..4674
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_unit     complement(918..919)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1816..1824
                     /label="Kozak"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_unit     5569..5603
                     /label="3'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1554..1572
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3095..3322
                     /label="SV40_pA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    887..917
                     /label="31bp spacer"
                     /ApEinfo_revcolor=#43ffb8
                     /ApEinfo_fwdcolor=#43ffb8
     TATA_signal     1696..1700
                     /label="TATA signal"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     repeat_unit     complement(921..936)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1591..1609
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        3413..3958
                     /label="EF1\promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3095..3322
                     /label="SV40_pA_Term"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1825..2970
                     /label="Gaolf"
                     /ApEinfo_revcolor=#82ca9d
                     /ApEinfo_fwdcolor=#82ca9d
     enhancer        1112..1343
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        1389..1691
                     /label="TRE Pitt"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4680..4815
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     enhancer        complement(5064..5294)
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1517..1535
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(7258..8115)
                     /label="Amp"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    1480..1498
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        1692..1791
                     /label="minimal CMV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
       61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
      121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
      181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
      241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
      301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
      361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT
      421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA
      481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC
      541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC
      601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG
      661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA
      721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG
      781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG
      841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG
      901 TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG
      961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA
     1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAC AAAAACTCAA
     1081 AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG CCCCCCCCCA AAGCCCCCAG
     1141 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC
     1201 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG
     1261 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA
     1321 CACCTGGGGG GATACGGGGA AAAGGCCTCC AcGGCCacta gtccaatcat aggGgtaccC
     1381 GACCCATCAA GTGCCACCTG ACGTCTCCCT ATCAGTGATA GAGAAGTCGA CACGTCTCGA
     1441 GCTCCCTATC AGTGATAGAG AAGGTACGTC TAGAACGTCT CCCTATCAGT GATAGAGAAG
     1501 TCGACACGTC TCGAGCTCCC TATCAGTGAT AGAGAAGGTA CGTCTAGAAC GTCTCCCTAT
     1561 CAGTGATAGA GAAGTCGACA CGTCTCGAGC TCCCTATCAG TGATAGAGAA GGTACGTCTA
     1621 GAACGTCTCC CTATCAGTGA TAGAGAAGTC GACACGTCTC GAGCTCCCTA TCAGTGATAG
     1681 AGAAGGTACC CCCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG
     1741 CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGGGAC CGATCCAGCC TGAGCTCGTT
     1801 TAGTGACCGt ctagaGCCGC CACCATGGGG TGTTTGGGCA ACAGCAGCAA GACCGCGGAA
     1861 GATCAGGGCG TAGATGAAAA AGAACGCCGC GAGGCCAACA AAAAGATCGA GAAGCAGTTG
     1921 CAGAAAGAGC GCCTGGCTTA CAAAGCAACT CACCGCCTGC TGCTTCTGGG GGCTGGTGAG
     1981 TCCGGGAAAA GCACTATCGT CAAACAGATG AGGATCCTGC ACGTCAATGG CTTCAACCCC
     2041 GAGGAAAAGA AGCAGAAAAT TCTGGACATC AGGAAAAATG TCAAAGATGC GATCGTGACA
     2101 ATCGTTTCAG CAATGAGTAC TATCATACCT CCAGTTCCAC TGGCCAACCC TGAGAACCAG
     2161 TTCCGGTCAG ATTATATCAA GAGCATAGCC CCTATCACTG ACTTTGAATA TTCCCAGGAG
     2221 TTCTTTGACC ATGTGAAGAA GCTGTGGGAC GATGAAGGAG TGAAGGCCTG CTTTGAGAGA
     2281 TCCAACGAGT ACCAGCTGAT CGACTGTGCA CAATACTTCC TGGAAAGGAT TGACAGTGTC
     2341 AGTCTGGTTG ACTACACACC CACAGACCAG GACCTGCTCA GATGCAGAGT GCTGACATCA
     2401 GGAATCTTTG AGACACGATT CCAAGTGGAC AAAGTGAACT TTCACATGTT TGATGTTGGA
     2461 GGCCAGAGAG ATGAGAGAAG AAAATGGATC CAGTGTTTTA ATGATGTCAC TGCGATCATT
     2521 TACGTGGCGG CCTGTAGTAG CTACAACATG GTGATCCGGG AAGATAACAA TACCAACAGA
     2581 CTTCGGGAAT CACTGGACCT GTTTGAAAGC ATCTGGAATA ACAGGTGGTT GCGAACCATT
     2641 TCTATCATCC TATTCTTGAA CAAACAAGAC ATGCTGGCAG AAAAAGTCTT GGCAGGGAAG
     2701 TCAAAAATCG AAGACTATTT CCCGGAGTAT GCCAATTATA CTGTCCCTGA AGATGCAACA
     2761 CCAGATGCGG GAGAAGATCC CAAAGTTACA AGAGCAAAGT TCTTTATCCG GGATCTGTTC
     2821 TTGAGGATCA GCACAGCCAC GGGTGATGGC AAACATTACT GCTACCCTCA CTTCACCTGC
     2881 GCCGTGGACA CAGAGAACAT CCGCAGAGTG TTCAACGATT GCCGTGACAT CATCCAGAGA
     2941 ATGCATCTCA AGCAGTACGA ACTCTTGTGA gaattcgcca cacaggcata gagtgtctgc
     3001 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
     3061 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
     3121 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     3181 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     3241 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3301 ccaaactcat caatgtatct tatcatgtct ggatccccag gaagctcctc tgtgtcctca
     3361 taaaccctaa cctcctctac ttgagaggac attccaatca taggcggccg cgaaggatct
     3421 gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     3481 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
     3541 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     3601 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga
     3661 agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc
     3721 cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg
     3781 ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga
     3841 gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg
     3901 tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc
     3961 tagatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     4021 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     4081 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     4141 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     4201 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     4261 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     4321 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     4381 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     4441 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     4501 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     4561 tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4621 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatGcgTTA
     4681 ACTaaacTtG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT
     4741 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT
     4801 ATCTTATCAT GTCTGGAATT GACTCAAATG ATGTCAATTA GTCTATCAGA AGCTcATCTG
     4861 GTCTCCCTTC CGGGGGACAA GACATCCCTG TTTAATATTT AAACAGCAGT GTTCCCAAAC
     4921 TGGGTTCTTA TATCCCTTGC TCTGGTCAAC CAGGTTGCAG GGTTTCCTGT CCTCACAGGA
     4981 ACGAAGTCCC TAAAGAAACA GTGGCAGCCA GGTTTAGCCC CGGAATTGAC TGGATTCCTT
     5041 TTTTAGGGCC CATTGGTATG GCTTTTTCCC CGTATCCCCC CAGGTGTCTG CAGGCTCAAA
     5101 GAGCAGCGAG AAGCGTTCAG AGGAAAGCGA TCCCGTGCCA CCTTCCCCGT GCCCGGGCTG
     5161 TCCCCGCACG CTGCCGGCTC GGGGATGCGG GGGGAGCGCC GGACCGGAGC GGAGCCCCGG
     5221 GCGGCTCGCT GCTGCCCCCT AGCGGGGGAG GGACGTAATT ACATCCCTGG GGGCTTTGGG
     5281 GGGGGGCTGT CCCTGATATC TATAACAAGA AAATATATAT ATAATAAGTT ATCACGTAAG
     5341 TAGAACATGA AATAACAATA TAATTATCGT ATGAGTTAAA TCTTAAAAGT CACGTAAAAG
     5401 ATAATCATGC GTCATTTTGA CTCACGCGGT CGTTATAGTT CAAAATCAGT GACACTTACC
     5461 GCATTGACAA GCACGCCTCA CGGGAGCTCC AAGCGGCGAC TGAGATGTCC TAAATGCACA
     5521 GCGACGGATT CGCGCTATTT AGAAAGAGAG AGCAATATTT CAAGAATGCA TGCGTCAATT
     5581 TTACGCAGAC TATCTTTCTA GGGTTAATCT AGCTGCATCA GGATCATATC GTCGGGTCTT
     5641 TTTTCCGGCT CAGTCATCGC CCAAGCTGGC GCTATCTGGG CATCGGGGAG GAAGAAGCCC
     5701 GTGCCTTTTC CCGCGAGGTT GAAGCGGCAT GGAAAGAGTT TGCCGAGGAT GACTGCTGCT
     5761 GCATTGACGT TGAGCGAAAA CGCACGTTTA CCATGATGAT TCGGGAAGGT GTGGCCATGC
     5821 ACGCCTTTAA CGGTGAACTG TTCGTTCAGG CCACCTGGGA TACCAGTTCG TCGCGGCTTT
     5881 TCCGGACACA GTTCCGGATG GTCAGCCCGA AGCGCATCAG CAACCCGAAC AATACCGGCG
     5941 ACAGCCGGAA CTGCCGTGCC GGTGTGCAGA TTAATGACAG CGGTGCGGCG CTGGGATATT
     6001 ACGTCAGCGA GGACGGGTAT CCTGGCTGGA TGCCGCAGAA ATGGACATGG ATACCCCGTG
     6061 AGTTACCCGG CGGGCGCGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG
     6121 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG
     6181 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC
     6241 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT
     6301 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
     6361 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
     6421 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
     6481 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     6541 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     6601 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     6661 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     6721 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     6781 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     6841 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     6901 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
     6961 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     7021 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     7081 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
     7141 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
     7201 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
     7261 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
     7321 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
     7381 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
     7441 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
     7501 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
     7561 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC
     7621 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
     7681 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
     7741 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
     7801 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
     7861 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
     7921 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
     7981 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
     8041 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
     8101 ATGTTGAATA CTCAT
//