LOCUS       Ric8b_Integration_Vecto 8646 bp ds-DNA     circular     10-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1810..1818
                     /label="Kozak"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_unit     complement(887..917)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     repeat_unit     complement(921..936)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(6653..6672)
                     /label="pLac(F)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    1665..1683
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     TATA_signal     1696..1700
                     /label="TATA signal"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    887..917
                     /label="31bp spacer"
                     /ApEinfo_revcolor=#43ffb8
                     /ApEinfo_fwdcolor=#43ffb8
     misc_feature    5211..5346
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1554..1572
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     repeat_unit     complement(874..886)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        1389..1691
                     /label="TRE Pitt"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     repeat_unit     6100..6134
                     /label="3'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     repeat_unit     complement(918..919)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1443..1461
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    1517..1535
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4495..5091
                     /label="puro"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1628..1646
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     gene            1819..3498
                     /label="Ric8b "
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3626..3853
                     /label="SV40_pA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     enhancer        complement(5595..5825)
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1406..1424
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3626..3853
                     /label="SV40_pA_Term"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    1480..1498
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        1692..1791
                     /label="minimal CMV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        3944..4489
                     /label="EF1\promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(7789..8646)
                     /label="Amp"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    1591..1609
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     enhancer        1112..1343
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    5101..5205
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
       61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
      121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
      181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
      241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
      301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
      361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT
      421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA
      481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC
      541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC
      601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG
      661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA
      721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG
      781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG
      841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG
      901 TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG
      961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA
     1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAC AAAAACTCAA
     1081 AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG CCCCCCCCCA AAGCCCCCAG
     1141 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC
     1201 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG
     1261 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA
     1321 CACCTGGGGG GATACGGGGA AAAGGCCTCC AcGGCCacta gtccaatcat aggGgtaccC
     1381 GACCCATCAA GTGCCACCTG ACGTCTCCCT ATCAGTGATA GAGAAGTCGA CACGTCTCGA
     1441 GCTCCCTATC AGTGATAGAG AAGGTACGTC TAGAACGTCT CCCTATCAGT GATAGAGAAG
     1501 TCGACACGTC TCGAGCTCCC TATCAGTGAT AGAGAAGGTA CGTCTAGAAC GTCTCCCTAT
     1561 CAGTGATAGA GAAGTCGACA CGTCTCGAGC TCCCTATCAG TGATAGAGAA GGTACGTCTA
     1621 GAACGTCTCC CTATCAGTGA TAGAGAAGTC GACACGTCTC GAGCTCCCTA TCAGTGATAG
     1681 AGAAGGTACC CCCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG
     1741 CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGGGAC CGATCCAGCC TGAGCTCGTT
     1801 TAGTGACCGG CCGCCACCat ggatgaagag cgcgccctct acatcgtccg ggccggcgag
     1861 gcgggggcta tcgagcgggt cctgagggat tacagcgaca agcatagggc tactttcaaa
     1921 tttgaatcag cagatgaaga taaaagaaag aaactctgtg aaggcatatt taaggtcctt
     1981 gtcaaggagg tcccaacgac atgccaagtg tcctgcctgg aagtactccg cattctctcc
     2041 agagacaaaa agatcttagt tcctgtaaca actaaggaaa atatgcaaat actgctgaga
     2101 ctagccaagc tccacgagtc agacgattct ttggagaagg tgtcagagtt cccagttatt
     2161 gtggaatcat tgaagtgtct gtgtaacatc gtgttcaaca gtcagatggc acagcagctt
     2221 agcctggaac tgaatctcgc tgcaaagctc tgtaacctcc taaggaagtg caaggaccga
     2281 aaatttatca atgacataaa gtgctttgac ttgcgcttgc tcttcgtctt gtcacttttg
     2341 cacactgaca tcaggtcaca attgcgctat gagctccagg gactcccgct gctaacccag
     2401 atcttggaaa gtgcctttag catcaagtgg accgatgagt atgagtcggc catagaccat
     2461 aatggacctc ctctctcacc tcaggagaca gactgtgcca ttgaggccct caaagctctg
     2521 ttcaatgtga cggtagacag ttggaaggtg cataaagaga gcgattctca tcagtttcgt
     2581 gtcatggcag ctgtccttcg ccattgttta ctaattgtag gtccaactga agacaaaacc
     2641 gaagagctgc acagtaatgc cgtcaacctt ttaagcaatg ttccagtctc ttgtttggat
     2701 gttctcattt gtccattaac ccatgaagaa acagcccaag aggcagcaac tctagatgaa
     2761 ctgcccagtg ataaaacaac ggagaaagac acagctttga aaaacagtac catggtatac
     2821 aatggcatga atatggaggc cattcacgtt ttactcaatt ttatggagaa gagaattgat
     2881 aagggaagca gctatagaga gggtctaact ccagttctca gcttattaac agaatgttcc
     2941 cgagcccatc ggaacatccg aaaatttctc aaagatcagg ttttaccgcc tttgagagat
     3001 gtgacaaatc gaccagaagt cggctcaacc gtgagaaata agctggttcg tctcatgaca
     3061 catgttgacc tcggagtcaa gcagattgct gctgagttcc tttttgtcct ttgcaaagag
     3121 agagtggata gcctgctgaa atacactggc tatgggaacg ctgcaggact cttggcggcc
     3181 aggggcctct tggctggagg aagaggagat aattggtact cagaggacga ggacacggac
     3241 actgaagaat acaaaaacgc aaaaccaaac attaatctta tcactggtca tttagaggaa
     3301 ccaatgccaa accctataga tgaaatgaca gaagaacaaa aagaatatga agccatgaaa
     3361 cttgtcaaca tgcttgataa actttccaga gaggagttgc ttaaaccaat gggactaaaa
     3421 cctgacggga caataacgcc tttggaggaa gcactcagcc agtattctgt catcgaagag
     3481 accagctcgg acacagacTG Agaattcgcc acacaggcat agagtgtctg ctattaataa
     3541 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     3601 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     3661 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     3721 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3781 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3841 tcaatgtatc ttatcatgtc tggatcccca ggaagctcct ctgtgtcctc ataaacccta
     3901 acctcctcta cttgagagga cattccaatc ataggcggcc gcgaaggatc tgcgatcgct
     3961 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     4021 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     4081 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     4141 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag
     4201 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt
     4261 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg
     4321 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta
     4381 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt
     4441 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagatgacc
     4501 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     4561 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     4621 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     4681 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     4741 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     4801 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     4861 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     4921 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     4981 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     5041 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagtcgac
     5101 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5161 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatGcgTT AACTaaacTt
     5221 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA
     5281 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA
     5341 TGTCTGGAAT TGACTCAAAT GATGTCAATT AGTCTATCAG AAGCTcATCT GGTCTCCCTT
     5401 CCGGGGGACA AGACATCCCT GTTTAATATT TAAACAGCAG TGTTCCCAAA CTGGGTTCTT
     5461 ATATCCCTTG CTCTGGTCAA CCAGGTTGCA GGGTTTCCTG TCCTCACAGG AACGAAGTCC
     5521 CTAAAGAAAC AGTGGCAGCC AGGTTTAGCC CCGGAATTGA CTGGATTCCT TTTTTAGGGC
     5581 CCATTGGTAT GGCTTTTTCC CCGTATCCCC CCAGGTGTCT GCAGGCTCAA AGAGCAGCGA
     5641 GAAGCGTTCA GAGGAAAGCG ATCCCGTGCC ACCTTCCCCG TGCCCGGGCT GTCCCCGCAC
     5701 GCTGCCGGCT CGGGGATGCG GGGGGAGCGC CGGACCGGAG CGGAGCCCCG GGCGGCTCGC
     5761 TGCTGCCCCC TAGCGGGGGA GGGACGTAAT TACATCCCTG GGGGCTTTGG GGGGGGGCTG
     5821 TCCCTGATAT CTATAACAAG AAAATATATA TATAATAAGT TATCACGTAA GTAGAACATG
     5881 AAATAACAAT ATAATTATCG TATGAGTTAA ATCTTAAAAG TCACGTAAAA GATAATCATG
     5941 CGTCATTTTG ACTCACGCGG TCGTTATAGT TCAAAATCAG TGACACTTAC CGCATTGACA
     6001 AGCACGCCTC ACGGGAGCTC CAAGCGGCGA CTGAGATGTC CTAAATGCAC AGCGACGGAT
     6061 TCGCGCTATT TAGAAAGAGA GAGCAATATT TCAAGAATGC ATGCGTCAAT TTTACGCAGA
     6121 CTATCTTTCT AGGGTTAATC TAGCTGCATC AGGATCATAT CGTCGGGTCT TTTTTCCGGC
     6181 TCAGTCATCG CCCAAGCTGG CGCTATCTGG GCATCGGGGA GGAAGAAGCC CGTGCCTTTT
     6241 CCCGCGAGGT TGAAGCGGCA TGGAAAGAGT TTGCCGAGGA TGACTGCTGC TGCATTGACG
     6301 TTGAGCGAAA ACGCACGTTT ACCATGATGA TTCGGGAAGG TGTGGCCATG CACGCCTTTA
     6361 ACGGTGAACT GTTCGTTCAG GCCACCTGGG ATACCAGTTC GTCGCGGCTT TTCCGGACAC
     6421 AGTTCCGGAT GGTCAGCCCG AAGCGCATCA GCAACCCGAA CAATACCGGC GACAGCCGGA
     6481 ACTGCCGTGC CGGTGTGCAG ATTAATGACA GCGGTGCGGC GCTGGGATAT TACGTCAGCG
     6541 AGGACGGGTA TCCTGGCTGG ATGCCGCAGA AATGGACATG GATACCCCGT GAGTTACCCG
     6601 GCGGGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT
     6661 CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG
     6721 AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT
     6781 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG
     6841 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC
     6901 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
     6961 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT
     7021 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     7081 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     7141 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     7201 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     7261 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     7321 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     7381 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     7441 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     7501 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     7561 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
     7621 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     7681 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
     7741 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
     7801 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
     7861 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
     7921 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
     7981 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
     8041 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
     8101 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
     8161 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
     8221 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
     8281 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
     8341 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
     8401 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
     8461 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
     8521 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
     8581 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
     8641 ACTCAT
//