LOCUS       RTP1S_Integration_Vecto 7653 bp ds-DNA     circular     10-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1816..1824
                     /label="Kozak"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1443..1461
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4218..4353
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     repeat_unit     complement(918..919)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1517..1535
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    887..917
                     /label="31bp spacer"
                     /ApEinfo_revcolor=#43ffb8
                     /ApEinfo_fwdcolor=#43ffb8
     repeat_unit     complement(887..917)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1591..1609
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1825..2508
                     /label="RTP1S"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    1554..1572
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2633..2860
                     /label="SV40_pA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    1665..1683
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     enhancer        complement(4602..4832)
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_unit     5107..5141
                     /label="3'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2633..2860
                     /label="SV40_pA_Term"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     enhancer        1112..1343
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_unit     complement(874..886)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        2951..3496
                     /label="EF1\promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(6796..7653)
                     /label="Amp"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        1389..1691
                     /label="TRE Pitt"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     repeat_unit     complement(921..936)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    1480..1498
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     TATA_signal     1696..1700
                     /label="TATA signal"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     promoter        1692..1791
                     /label="minimal CMV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4108..4212
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1406..1424
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    1628..1646
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3502..4098
                     /label="puro"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
       61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
      121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
      181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
      241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
      301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
      361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT
      421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA
      481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC
      541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC
      601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG
      661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA
      721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG
      781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG
      841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG
      901 TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG
      961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA
     1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAC AAAAACTCAA
     1081 AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG CCCCCCCCCA AAGCCCCCAG
     1141 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC
     1201 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG
     1261 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA
     1321 CACCTGGGGG GATACGGGGA AAAGGCCTCC AcGGCCacta gtccaatcat aggGgtaccC
     1381 GACCCATCAA GTGCCACCTG ACGTCTCCCT ATCAGTGATA GAGAAGTCGA CACGTCTCGA
     1441 GCTCCCTATC AGTGATAGAG AAGGTACGTC TAGAACGTCT CCCTATCAGT GATAGAGAAG
     1501 TCGACACGTC TCGAGCTCCC TATCAGTGAT AGAGAAGGTA CGTCTAGAAC GTCTCCCTAT
     1561 CAGTGATAGA GAAGTCGACA CGTCTCGAGC TCCCTATCAG TGATAGAGAA GGTACGTCTA
     1621 GAACGTCTCC CTATCAGTGA TAGAGAAGTC GACACGTCTC GAGCTCCCTA TCAGTGATAG
     1681 AGAAGGTACC CCCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG
     1741 CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGGGAC CGATCCAGCC TGAGCTCGTT
     1801 TAGTGACCGt ctagaGCCGC CACCATGTGT AAGAGTGTGA CCACAGGTGA GTGGAAGAAG
     1861 GTCTTCTACG AGAAGATGGA GGAGGTGAAG CCAGCGGACA GCTGGGACTT CATCATAGAC
     1921 CCCAACCTCA AGCACAATGT GTTGGCCCCT GGCTGGAAGC AGTACCTGGA ACTTCATGCC
     1981 TCAGGCAGGT TCCACTGTTC CTGGTGCTGG CACACCTGGC AGTCACCCCA TGTAGTCATC
     2041 CTCTTCCACA TGTACCTGGA CAAGGCTCAG CGCGCTGGTT CGGTGCGCAT GCGTGTGTTC
     2101 AAGCAGCTCT GCTACGAGTG CGGTACAGCA CGGCTGGATG AGTCCAGCAT GCTGGAGGAG
     2161 AACATCGAAA GCCTGGTGGA CAACCTCATC ACCAGTTTGC GAGAGCAGTG CTACGGGGAG
     2221 CGTGGTGGCC ACTACCGCAT CCATGTGGCC AGCCGGCAGG ACAACCGGCG ACACCGCGGA
     2281 GAGTTCTGCG AGGCCTGCCA GGAAGGCATC GTGCACTGGA AGCCCAGTGA GAAGCTGCTG
     2341 GAGGAGGAGG CGACCACCTA CACCTTCTCC CGTGCTCCCA GCCCCACCAA ACCGCAGGCT
     2401 GAAACAGGCT CAGGCTGCAA CTTCTGCTCC ATTCCCTGGT GCTTATTTTG GGCCACGGTT
     2461 TTGATGCTCA TCATCTACCT GCAATTCTCC TTCCGTACTT CTGTCTGAga attcgccaca
     2521 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     2581 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     2641 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     2701 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     2761 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2821 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccccagga
     2881 agctcctctg tgtcctcata aaccctaacc tcctctactt gagaggacat tccaatcata
     2941 ggcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc
     3001 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt
     3061 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     3121 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3181 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc
     3241 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg
     3301 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt
     3361 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc
     3421 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc
     3481 tgtgaccggc gcctacgcta gatgaccgag tacaagccca cggtgcgcct cgccacccgc
     3541 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     3601 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     3661 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     3721 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     3781 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     3841 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     3901 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     3961 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     4021 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     4081 acccgcaagc ccggtgcctg agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     4141 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4201 cctttgtatc atGcgTTAAC TaaacTtGTT TATTGCAGCT TATAATGGTT ACAAATAAAG
     4261 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT
     4321 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGAATTGA CTCAAATGAT GTCAATTAGT
     4381 CTATCAGAAG CTcATCTGGT CTCCCTTCCG GGGGACAAGA CATCCCTGTT TAATATTTAA
     4441 ACAGCAGTGT TCCCAAACTG GGTTCTTATA TCCCTTGCTC TGGTCAACCA GGTTGCAGGG
     4501 TTTCCTGTCC TCACAGGAAC GAAGTCCCTA AAGAAACAGT GGCAGCCAGG TTTAGCCCCG
     4561 GAATTGACTG GATTCCTTTT TTAGGGCCCA TTGGTATGGC TTTTTCCCCG TATCCCCCCA
     4621 GGTGTCTGCA GGCTCAAAGA GCAGCGAGAA GCGTTCAGAG GAAAGCGATC CCGTGCCACC
     4681 TTCCCCGTGC CCGGGCTGTC CCCGCACGCT GCCGGCTCGG GGATGCGGGG GGAGCGCCGG
     4741 ACCGGAGCGG AGCCCCGGGC GGCTCGCTGC TGCCCCCTAG CGGGGGAGGG ACGTAATTAC
     4801 ATCCCTGGGG GCTTTGGGGG GGGGCTGTCC CTGATATCTA TAACAAGAAA ATATATATAT
     4861 AATAAGTTAT CACGTAAGTA GAACATGAAA TAACAATATA ATTATCGTAT GAGTTAAATC
     4921 TTAAAAGTCA CGTAAAAGAT AATCATGCGT CATTTTGACT CACGCGGTCG TTATAGTTCA
     4981 AAATCAGTGA CACTTACCGC ATTGACAAGC ACGCCTCACG GGAGCTCCAA GCGGCGACTG
     5041 AGATGTCCTA AATGCACAGC GACGGATTCG CGCTATTTAG AAAGAGAGAG CAATATTTCA
     5101 AGAATGCATG CGTCAATTTT ACGCAGACTA TCTTTCTAGG GTTAATCTAG CTGCATCAGG
     5161 ATCATATCGT CGGGTCTTTT TTCCGGCTCA GTCATCGCCC AAGCTGGCGC TATCTGGGCA
     5221 TCGGGGAGGA AGAAGCCCGT GCCTTTTCCC GCGAGGTTGA AGCGGCATGG AAAGAGTTTG
     5281 CCGAGGATGA CTGCTGCTGC ATTGACGTTG AGCGAAAACG CACGTTTACC ATGATGATTC
     5341 GGGAAGGTGT GGCCATGCAC GCCTTTAACG GTGAACTGTT CGTTCAGGCC ACCTGGGATA
     5401 CCAGTTCGTC GCGGCTTTTC CGGACACAGT TCCGGATGGT CAGCCCGAAG CGCATCAGCA
     5461 ACCCGAACAA TACCGGCGAC AGCCGGAACT GCCGTGCCGG TGTGCAGATT AATGACAGCG
     5521 GTGCGGCGCT GGGATATTAC GTCAGCGAGG ACGGGTATCC TGGCTGGATG CCGCAGAAAT
     5581 GGACATGGAT ACCCCGTGAG TTACCCGGCG GGCGCGCTTG GCGTAATCAT GGTCATAGCT
     5641 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT
     5701 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC
     5761 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG
     5821 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
     5881 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
     5941 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
     6001 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
     6061 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     6121 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     6181 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     6241 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     6301 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     6361 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     6421 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT
     6481 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     6541 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     6601 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     6661 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     6721 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     6781 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
     6841 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
     6901 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT
     6961 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
     7021 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
     7081 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
     7141 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
     7201 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
     7261 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
     7321 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
     7381 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
     7441 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
     7501 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
     7561 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
     7621 AATAAGGGCG ACACGGAAAT GTTGAATACT CAT
//