LOCUS       RTP2_Integration_Vector 7647 bp ds-DNA     circular     10-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     repeat_unit     complement(921..936)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        1692..1791
                     /label="minimal CMV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3496..4092
                     /label="puro"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1591..1609
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    1517..1535
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    1554..1572
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2627..2854
                     /label="SV40_pA_Term"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    1443..1461
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1816..1824
                     /label="Kozak"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2627..2854
                     /label="SV40_pA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(6790..7647)
                     /label="Amp"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     enhancer        complement(4596..4826)
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1628..1646
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     repeat_unit     complement(887..917)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     TATA_signal     1696..1700
                     /label="TATA signal"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    4212..4347
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        1389..1691
                     /label="TRE Pitt"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4102..4206
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_unit     complement(874..886)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     enhancer        1112..1343
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_unit     5101..5135
                     /label="3'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     repeat_unit     complement(918..919)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1825..2499
                     /label="RTP2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    887..917
                     /label="31bp spacer"
                     /ApEinfo_revcolor=#43ffb8
                     /ApEinfo_fwdcolor=#43ffb8
     promoter        2945..3490
                     /label="EF1\promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    1406..1424
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    1665..1683
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    1480..1498
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
       61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
      121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
      181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
      241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
      301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
      361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT
      421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA
      481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC
      541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC
      601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG
      661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA
      721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG
      781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG
      841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG
      901 TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG
      961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA
     1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAC AAAAACTCAA
     1081 AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG CCCCCCCCCA AAGCCCCCAG
     1141 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC
     1201 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG
     1261 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA
     1321 CACCTGGGGG GATACGGGGA AAAGGCCTCC AcGGCCacta gtccaatcat aggGgtaccC
     1381 GACCCATCAA GTGCCACCTG ACGTCTCCCT ATCAGTGATA GAGAAGTCGA CACGTCTCGA
     1441 GCTCCCTATC AGTGATAGAG AAGGTACGTC TAGAACGTCT CCCTATCAGT GATAGAGAAG
     1501 TCGACACGTC TCGAGCTCCC TATCAGTGAT AGAGAAGGTA CGTCTAGAAC GTCTCCCTAT
     1561 CAGTGATAGA GAAGTCGACA CGTCTCGAGC TCCCTATCAG TGATAGAGAA GGTACGTCTA
     1621 GAACGTCTCC CTATCAGTGA TAGAGAAGTC GACACGTCTC GAGCTCCCTA TCAGTGATAG
     1681 AGAAGGTACC CCCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG
     1741 CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGGGAC CGATCCAGCC TGAGCTCGTT
     1801 TAGTGACCGt ctagaGCCGC CACCatgtgt acttcactga ccacgtgtga gtggaagaag
     1861 gtgttttatg agaaaatgga agtcgccaag cctgccgatt cctgggaact tatcatcgac
     1921 ccaaacctga agccatccga gctcgctccc gggtggaagc agtacctgga acaacacgct
     1981 tccggaaggt tccactgctc ttggtgctgg cacacttggc agtcagcaca tgtagtcatt
     2041 ctgttccaca tgtttctgga tagggctcaa cgggcaggca gcgtgcgcat gcgcgtcttt
     2101 aagcaactct gctacgagtg tggcacagcc cgacttgatg agtcttcaat gttggaagag
     2161 aacatcgagg gcctggtgga caatctcatc acgtctttgc gcgaacagtg ttacgaagag
     2221 gatggggggc aataccggat tcatgttgca tctcgccctg attcaggtcc acacagggca
     2281 gagttctgcg aggcatgtca ggaaggtatt gtgcattgga aaccctccga gaagctgctg
     2341 gaggaggagg ttacaacata tactagcgaa gcgtccaaac ctagagcaca agccgggagc
     2401 ggctacaact tcctttccct tcggtggtgc ctgttttggg cctcactgtg cctgctggtt
     2461 gtgtatttgc agttcagctt cttgagccct gccttttttT GAgaattcgc cacacaggca
     2521 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
     2581 taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
     2641 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2701 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2761 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2821 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcccc aggaagctcc
     2881 tctgtgtcct cataaaccct aacctcctct acttgagagg acattccaat cataggcggc
     2941 cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     3001 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg
     3061 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     3121 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     3181 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct
     3241 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct
     3301 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg
     3361 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg
     3421 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac
     3481 cggcgcctac gctagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     3541 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     3601 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     3661 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     3721 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     3781 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     3841 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     3901 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     3961 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     4021 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     4081 aagcccggtg cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     4141 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4201 tatcatGcgT TAACTaaacT tGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
     4261 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
     4321 ACTCATCAAT GTATCTTATC ATGTCTGGAA TTGACTCAAA TGATGTCAAT TAGTCTATCA
     4381 GAAGCTcATC TGGTCTCCCT TCCGGGGGAC AAGACATCCC TGTTTAATAT TTAAACAGCA
     4441 GTGTTCCCAA ACTGGGTTCT TATATCCCTT GCTCTGGTCA ACCAGGTTGC AGGGTTTCCT
     4501 GTCCTCACAG GAACGAAGTC CCTAAAGAAA CAGTGGCAGC CAGGTTTAGC CCCGGAATTG
     4561 ACTGGATTCC TTTTTTAGGG CCCATTGGTA TGGCTTTTTC CCCGTATCCC CCCAGGTGTC
     4621 TGCAGGCTCA AAGAGCAGCG AGAAGCGTTC AGAGGAAAGC GATCCCGTGC CACCTTCCCC
     4681 GTGCCCGGGC TGTCCCCGCA CGCTGCCGGC TCGGGGATGC GGGGGGAGCG CCGGACCGGA
     4741 GCGGAGCCCC GGGCGGCTCG CTGCTGCCCC CTAGCGGGGG AGGGACGTAA TTACATCCCT
     4801 GGGGGCTTTG GGGGGGGGCT GTCCCTGATA TCTATAACAA GAAAATATAT ATATAATAAG
     4861 TTATCACGTA AGTAGAACAT GAAATAACAA TATAATTATC GTATGAGTTA AATCTTAAAA
     4921 GTCACGTAAA AGATAATCAT GCGTCATTTT GACTCACGCG GTCGTTATAG TTCAAAATCA
     4981 GTGACACTTA CCGCATTGAC AAGCACGCCT CACGGGAGCT CCAAGCGGCG ACTGAGATGT
     5041 CCTAAATGCA CAGCGACGGA TTCGCGCTAT TTAGAAAGAG AGAGCAATAT TTCAAGAATG
     5101 CATGCGTCAA TTTTACGCAG ACTATCTTTC TAGGGTTAAT CTAGCTGCAT CAGGATCATA
     5161 TCGTCGGGTC TTTTTTCCGG CTCAGTCATC GCCCAAGCTG GCGCTATCTG GGCATCGGGG
     5221 AGGAAGAAGC CCGTGCCTTT TCCCGCGAGG TTGAAGCGGC ATGGAAAGAG TTTGCCGAGG
     5281 ATGACTGCTG CTGCATTGAC GTTGAGCGAA AACGCACGTT TACCATGATG ATTCGGGAAG
     5341 GTGTGGCCAT GCACGCCTTT AACGGTGAAC TGTTCGTTCA GGCCACCTGG GATACCAGTT
     5401 CGTCGCGGCT TTTCCGGACA CAGTTCCGGA TGGTCAGCCC GAAGCGCATC AGCAACCCGA
     5461 ACAATACCGG CGACAGCCGG AACTGCCGTG CCGGTGTGCA GATTAATGAC AGCGGTGCGG
     5521 CGCTGGGATA TTACGTCAGC GAGGACGGGT ATCCTGGCTG GATGCCGCAG AAATGGACAT
     5581 GGATACCCCG TGAGTTACCC GGCGGGCGCG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC
     5641 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG
     5701 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC
     5761 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG
     5821 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
     5881 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
     5941 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
     6001 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     6061 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     6121 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     6181 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     6241 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     6301 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     6361 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     6421 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG
     6481 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     6541 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     6601 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     6661 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     6721 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     6781 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     6841 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     6901 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC
     6961 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     7021 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     7081 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     7141 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     7201 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     7261 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     7321 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     7381 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     7441 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     7501 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     7561 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     7621 GGCGACACGG AAATGTTGAA TACTCAT
//