LOCUS       Exported                3630 bp ds-DNA     circular SYN 02-JUL-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 2, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     21..38
                     /label=T7 Promoter primer
                     /note="/ApEinfo_fwdcolor=#aecfff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#aecfff"
                     /note="color: #aecfff; direction: RIGHT"
     promoter        64..151
                     /label=Constitutive E. coli Promoter
                     /note="/ApEinfo_fwdcolor=#ff0306
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff0306"
                     /note="color: #ff0306"
     CDS             152..256
                     /codon_start=1
                     /product="N-terminal 6xHis tag and linker from pRSET-B"
                     /label=6xHis linker
                     /note="/ApEinfo_fwdcolor=#da1bff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#da1bff"
                     /note="color: #da1bff"
                     /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDNDPLE"
     CDS             257..277
                     /codon_start=1
                     /product="TEV protease cleavage site linker"
                     /label=TEV protease cleavage site
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /note="color: #00ffff"
                     /translation="ENLYFQG"
     CDS             278..976
                     /codon_start=1
                     /label=AvicFP3
                     /note="color: #993366"
                     /translation="MNCGALLFKQKIPFIVELDGDVNGQVFSVRGNGFGDGSTGKIEIK
                     FVCTTGEIPVAWESLVCSLAYGALVFTKYPSNINDFFKSTMPKGYIQERKITFENDGQY
                     ETRAEVTYENGALYNRVKLNGSGFRKDGNILGKKLDATSLGTCIYIMSNDQGNGLKGVY
                     NKAYKVIGGNRQLASHQQTHTPIGDGLVSIPDYHVMHNHTSCSKDPTEPRDHLILKESV
                     TAVDCQTAYM"
     terminator      999..1128
                     /label=T7 transcription terminator
                     /note="/ApEinfo_fwdcolor=#958eff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#958eff"
                     /note="color: #958eff"
     primer_bind     1031..1049
                     /label=T7 terminator primer
                     /note="/ApEinfo_fwdcolor=#aecfff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#aecfff"
                     /note="color: #aecfff; direction: RIGHT"
     rep_origin      1199..1654
                     /label=f1 origin
                     /note="/ApEinfo_fwdcolor=#7905dd
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#7905dd"
                     /note="color: #7905dd"
     misc_feature    1686..1790
                     /label=bla promoter
                     /note="/ApEinfo_fwdcolor=#fb9c07
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#fb9c07"
                     /note="color: #fb9c07"
     gene            1775..2635
                     /label=Ampicillin resistance gene
                     /note="/ApEinfo_fwdcolor=#b4587a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#b4587a"
                     /note="color: #b4587a; direction: RIGHT"
     rep_origin      2784..3426
                     /label=pUC origin
                     /note="/ApEinfo_fwdcolor=#3e8995
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#3e8995"
                     /note="color: #3e8995"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaatcaccg agtttattct tgacacctga tgcgatgaat gatataatag gaaagtactg
      121 ttttgtttaa ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca
      181 tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg acgatgacga
      241 taacgatccg ctcgaggaaa acctgtactt ccagggtatg aactgcggtg ctctgctgtt
      301 caagcagaag atccctttca tcgttgaact ggacggtgac gttaacggtc aggttttctc
      361 tgttcgtggt aacggtttcg gtgacggttc taccggtaag atcgaaatca agttcgtttg
      421 caccaccggt gaaatccctg ttgcttggga atctctggtt tgctctctgg cttacggtgc
      481 tctggttttc accaagtacc cttctaacat caacgacttc ttcaagtcta ccatgcctaa
      541 gggttacatc caggaacgta agatcacctt cgaaaacgac ggtcagtacg aaacccgtgc
      601 tgaagttacc tacgaaaacg gtgctctgta caaccgtgtt aagctgaacg gttctggttt
      661 ccgtaaggac ggtaacatcc tgggtaagaa gctggacgct acctctctgg gtacctgcat
      721 ctacatcatg tctaacgacc agggtaacgg tctgaagggt gtttacaaca aggcttacaa
      781 ggttatcggt ggtaaccgtc agctggcttc tcatcagcag acccataccc ctatcggtga
      841 cggtctggtt tctatccctg actaccatgt tatgcataac catacctctt gctctaagga
      901 ccctaccgaa cctcgtgacc atctgatcct gaaggaatct gttaccgctg ttgactgcca
      961 gaccgcttac atgtgacgtt tgatccggct gctaacaaag cccgaaagga agctgagttg
     1021 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     1081 aggggttttt tgctgaaagg aggaactata tccggatctg gcgtaatagc gaagaggccc
     1141 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     1201 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     1261 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     1321 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     1381 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     1441 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     1501 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     1561 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     1621 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1741 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1981 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2041 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2101 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2161 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2221 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2281 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     2341 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2401 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2461 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2521 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2581 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2641 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2701 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2941 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3421 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3481 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3541 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     3601 ccccgcgcgt tggccgattc attaatgcag
//
