LOCUS       Exported                3648 bp ds-DNA     circular SYN 02-JUL-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 2, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     21..38
                     /label=T7 Promoter primer
                     /note="/ApEinfo_fwdcolor=#aecfff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#aecfff"
                     /note="color: #aecfff; direction: RIGHT"
     promoter        64..151
                     /label=Constitutive E. coli Promoter
                     /note="/ApEinfo_fwdcolor=#ff0306
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff0306"
                     /note="color: #ff0306"
     CDS             152..256
                     /codon_start=1
                     /product="N-terminal 6xHis tag and linker from pRSET-B"
                     /label=6xHis linker
                     /note="/ApEinfo_fwdcolor=#da1bff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#da1bff"
                     /note="color: #da1bff"
                     /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDNDPLE"
     CDS             257..277
                     /codon_start=1
                     /product="TEV protease cleavage site linker"
                     /label=TEV protease cleavage site
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
                     /note="color: #00ffff"
                     /translation="ENLYFQG"
     CDS             278..994
                     /codon_start=1
                     /label=mAvicFP1
                     /note="color: #00ff00"
                     /translation="MSKGAELFNGIVPILIELNGDVHGHKFSVRGEGEGDAGSGKIEIK
                     FVCTTGTLPVPWPTLVTTLCYGVQCFTRYPEHMKQHDFYKSAMPDGYIQKRTISFQDDG
                     HYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGNKMEYNYNSHSVYVLSDKANNGIKV
                     NFKIRHNLKGEGIQLADHDQQNIPIGDGPVLLPDYHYLSTQTKITKDPNEKRDHMNLVE
                     FVTACGITHGMDELYK"
     terminator      1017..1146
                     /label=T7 transcription terminator
                     /note="/ApEinfo_fwdcolor=#958eff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#958eff"
                     /note="color: #958eff"
     primer_bind     1049..1067
                     /label=T7 terminator primer
                     /note="/ApEinfo_fwdcolor=#aecfff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#aecfff"
                     /note="color: #aecfff; direction: RIGHT"
     rep_origin      1217..1672
                     /label=f1 origin
                     /note="/ApEinfo_fwdcolor=#7905dd
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#7905dd"
                     /note="color: #7905dd"
     misc_feature    1704..1808
                     /label=bla promoter
                     /note="/ApEinfo_fwdcolor=#fb9c07
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#fb9c07"
                     /note="color: #fb9c07"
     gene            1793..2653
                     /label=Ampicillin resistance gene
                     /note="/ApEinfo_fwdcolor=#b4587a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#b4587a"
                     /note="color: #b4587a; direction: RIGHT"
     rep_origin      2802..3444
                     /label=pUC origin
                     /note="/ApEinfo_fwdcolor=#3e8995
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#3e8995"
                     /note="color: #3e8995"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaatcaccg agtttattct tgacacctga tgcgatgaat gatataatag gaaagtactg
      121 ttttgtttaa ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca
      181 tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg acgatgacga
      241 taacgatccg ctcgaggaaa acctgtactt ccagggtatg tctaagggtg ctgaactgtt
      301 caacggtatc gttcctatcc tgatcgaact gaacggtgac gttcatggtc ataagttctc
      361 tgttcgtggt gaaggtgaag gtgacgctgg ttctggtaag atcgaaatca agttcgtttg
      421 caccaccggt accctgcctg ttccttggcc taccctggtt accaccttat gctacggtgt
      481 tcagtgcttc acccgttacc ctgaacatat gaagcagcat gacttctaca agtctgctat
      541 gcctgacggt tacatccaga agcgtaccat ctctttccag gacgacggtc attacaagac
      601 ccgtgctgaa gttaagttcg aaggtgacac cctggttaac cgtatcgaac tgaagggtat
      661 cgacttcaag gaagacggta acatcctggg taacaagatg gaatacaact acaactctca
      721 ttctgtttac gttctgtctg acaaggctaa caacggtatc aaggttaact tcaagatccg
      781 tcataacctg aagggtgaag gtatccagct ggctgaccat gaccagcaga acatccctat
      841 cggtgacggt cctgttctgc tgcctgacta ccattacctg tctacccaga ccaagatcac
      901 caaggaccct aacgaaaagc gtgaccatat gaacctggtt gaattcgtta ccgcttgcgg
      961 tatcacccat ggtatggacg aactgtacaa gtgacgtttg atccggctgc taacaaagcc
     1021 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     1081 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatctggc
     1141 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     1201 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1261 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     1321 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     1381 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     1441 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     1501 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     1561 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     1621 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     1681 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1741 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1801 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1861 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1921 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1981 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2041 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2101 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2161 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2221 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2281 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2341 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2401 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2461 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2521 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2581 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2641 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2701 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2761 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2821 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2881 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2941 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3001 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3061 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3121 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3181 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3241 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3301 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3361 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3421 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3481 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3541 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3601 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcag
//
