LOCUS pSL1211 12951 bp ds-DNA circular 03-JUL-2019 SOURCE ORGANISM COMMENT pSL0999+Step 1 (XbaI-NheI) from 1 to 11240 COMMENT pSL0999 from 1 to 11836 COMMENT pSL0753 from 1 to 10165 COMMENT pSL0444 from 1 to 5890 COMMENT H:/Kappe Lab Projects and Data/C-Term Tags(Head-to-Tail)/pSL0281+GFPmut2+PbDHFR3'UTR (KpnI-KasI).ape from 1to 5309 COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 2178..2733 /locus_tag="PyBiP 3'UTR" /label="PyBiP 3'UTR" /ApEinfo_label="PyBiP 3'UTR" /ApEinfo_fwdcolor="#c4ffff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature join(11349..11658,11663..11952) /vntifkey="21" /locus_tag="PbEF1a-A\5'UTR" /label="PbEF1a-A\5'UTR" /ApEinfo_label="PbEF1a-A\5'UTR" /ApEinfo_fwdcolor="#eb0214" /ApEinfo_revcolor="#eb0214" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2738..3417 /locus_tag="PyDHFR promoter" /label="PyDHFR promoter" /ApEinfo_label="PyDHFR promoter" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature join(7..8,10..124,126..215) /locus_tag="PyALBA4 KO HDR (2x100bp)" /label="PyALBA4 KO HDR (2x100bp)" /ApEinfo_label="PyALBA4 KO HDR (2x100bp)" /ApEinfo_fwdcolor="#0000ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 7..112 /locus_tag="PyALBA4 HDR Template - 5' side" /label="PyALBA4 HDR Template - 5' side" /ApEinfo_label="PyALBA4 HDR Template - 5' side" /ApEinfo_fwdcolor="#0080c0" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 113..130 /locus_tag="Barcode for HDR Template" /label="Barcode for HDR Template" /ApEinfo_label="Barcode for HDR Template" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1949..1954 /locus_tag="spacer 1" /label="spacer 1" /ApEinfo_label="spacer 1" /ApEinfo_fwdcolor="#a7a7a7" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3424..7692 /locus_tag="Cas9" /label="Cas9" /ApEinfo_label="Cas9" /ApEinfo_fwdcolor="#f160ff" /ApEinfo_revcolor="#f160ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 12523..12951 /locus_tag="PyEF1a 3'UTR" /label="PyEF1a 3'UTR" /ApEinfo_label="PyEF1a 3'UTR" /ApEinfo_fwdcolor="#d2ffff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(1738..1743) /locus_tag="PyALBA4-5' sgRNA" /label="PyALBA4-5' sgRNA" /ApEinfo_label="PyALBA4-5' sgRNA" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1998..2017 /locus_tag="PyALBA4KO-3' sgRNA" /label="PyALBA4KO-3' sgRNA" /ApEinfo_label="PyALBA4KO-3' sgRNA" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 7734..8412 /locus_tag="GFPmut2" /label="GFPmut2" /ApEinfo_label="GFPmut2" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 7645..7692 /locus_tag="C-terminal NLS from importin alpha-nucleoplasmin" /label="C-terminal NLS from importin alpha-nucleoplasmin" /ApEinfo_label="C-terminal NLS from importin alpha-nucleoplasmin" /ApEinfo_fwdcolor="#ff00ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2018..2094 /locus_tag="Sp guide RNA scaffold" /label="Sp guide RNA scaffold" /ApEinfo_label="Sp guide RNA scaffold" /ApEinfo_fwdcolor="#4f4fff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1961..1997 /locus_tag="Hammerhead Ribozyme" /label="Hammerhead Ribozyme" /ApEinfo_label="Hammerhead Ribozyme" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 8419..8866 /locus_tag="PbDHFR/TS\3'UTR" /label="PbDHFR/TS\3'UTR" /ApEinfo_label="PbDHFR/TS\3'UTR" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2098..2165 /locus_tag="HDV Ribozyme" /label="HDV Ribozyme" /ApEinfo_label="HDV Ribozyme" /ApEinfo_fwdcolor="#ffa042" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3424..3543 /locus_tag="N-terminal NLS from TALEN construct pBlueTAL" /label="N-terminal NLS from TALEN construct pBlueTAL" /ApEinfo_label="N-terminal NLS from TALEN construct pBlueTAL" /ApEinfo_fwdcolor="#ff0080" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(1955..1960) /locus_tag="PyALBA4KO-3' sgRNA(1)" /label="PyALBA4KO-3' sgRNA(1)" /ApEinfo_label="PyALBA4KO-3' sgRNA" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 10263..10945 /locus_tag="ColE1 origin" /label="ColE1 origin" /ApEinfo_label="ColE1 origin" /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1781..1800 /locus_tag="PyALBA4-5' sgRNA(1)" /label="PyALBA4-5' sgRNA(1)" /ApEinfo_label="PyALBA4-5' sgRNA" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 9506..10165 /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="yellow" /ApEinfo_revcolor="yellow" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3568..3570 /locus_tag="D10 (substitute D10A to make nickase)" /label="D10 (substitute D10A to make nickase)" /ApEinfo_label="D10 (substitute D10A to make nickase)" /ApEinfo_fwdcolor="#ff80c0" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature join(11349..11658,11663..11947) /locus_tag="PbEF1a 5'UTR" /label="PbEF1a 5'UTR" /ApEinfo_label="PbEF1a 5'UTR" /ApEinfo_fwdcolor="#804040" /ApEinfo_revcolor="#804040" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 131..174 /locus_tag="PyALBA4 ORF" /label="PyALBA4 ORF" /ApEinfo_label="PyALBA4 ORF" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 131..215 /locus_tag="PyALBA4 HDR Template - 3' side" /label="PyALBA4 HDR Template - 3' side" /ApEinfo_label="PyALBA4 HDR Template - 3' side" /ApEinfo_fwdcolor="#8080c0" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1801..1877 /locus_tag="Sp guide RNA scaffold(1)" /label="Sp guide RNA scaffold(1)" /ApEinfo_label="Sp guide RNA scaffold" /ApEinfo_fwdcolor="#4f4fff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3499..3522 /locus_tag="SV40 NLS" /label="SV40 NLS" /ApEinfo_label="SV40 NLS" /ApEinfo_fwdcolor="#ff80c0" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1744..1780 /locus_tag="Hammerhead Ribozyme(1)" /label="Hammerhead Ribozyme(1)" /ApEinfo_label="Hammerhead Ribozyme" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1881..1948 /locus_tag="HDV Ribozyme(1)" /label="HDV Ribozyme(1)" /ApEinfo_label="HDV Ribozyme" /ApEinfo_fwdcolor="#ffa042" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2166..2171 /locus_tag="spacer" /label="spacer" /ApEinfo_label="spacer" /ApEinfo_fwdcolor="#c0c0c0" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 224..1731 /locus_tag="PyGAPDH promoter" /label="PyGAPDH promoter" /ApEinfo_label="PyGAPDH promoter" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 11953..12516 /locus_tag="HsDHFR" /label="HsDHFR" /ApEinfo_label="HsDHFR" /ApEinfo_fwdcolor="#ff8080" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 ggtaccGGGA CACTTTTGAA ATATCATCAA TTGATAAATA TATATATATT CTATAAATAC 61 ATATATATAA TATAGTATAT ACCTATAGTT AATATTTATT GCGAAACTCA ACgcgggcgg 121 cggcgccggt CAACTGGACT TCAAGTTTTT AATCATAATG AAAAAGAACA ATAAAAATAT 181 ATTATACATG TAGGATAATT TTATTTTATT GTTTCgcggc cgcCAAACAC ATATATAACT 241 GAAACTGGAA GCATTCAATC ATTTTAATAA CCCCAAAACA TATTTTGTTC CTATTATATC 301 TTATATGTAA AATAATGAAA TAGTTAAAAT ATGTATATTG CATATATGTG TATGCTATAA 361 TTAACAGCTA TGGAAAAGAG AACACTATGA AGTATATTAT TCAAACCCCC CAAAAAAGTA 421 AGAAAATTTT AATATGTATT TAATTTATGA ATATAAATAT GATTTTCAAA GAAATTTAAA 481 AATCCCTTTC ACTAATAAAT AGTGTATTCA CAAATGCGAC TTATATTCAC AGCGTGCAAT 541 GCTGCTATAT TACTATGAGA ATAACCACTT TTTTTTATAG CCCTAGTAAA CTTGCAAAAA 601 TATATAATAA AGTATTCCTT TTAAAATTAC TTATATATGT ATATTTTAAA TTAATTTTTA 661 ATGCTACAAT AAAATGGCTT AAGTTTTTTC ATAGTATATA AAAATTAGCA ATTTTAAATG 721 CTATATATAT GCGGAAAGGA AAGTATATTA TCCGAAAATG AAGTGAAAAA ATAAATTAGT 781 ATGGATAATA TATAATAGCA ATTTAATAAA AACTGCAAAT GATTATAATA GCAAAAATGT 841 TGCATATTAA AGAAGAATTA TAAAAATAAC CACCGAAGCC CCTAATGTAA AATATAAGCA 901 CCTTAAATCA CATAAAATAA ATAATATAGT TATTACGTCC CCTTTCCTTA TTTAGCACTT 961 ATACAATATA TAATTAACGA TATATTAAAT TTATATATAG TTTTTTATTA AAAATATATA 1021 TTTTATAAAA TAACGTGCCT CTAAATAAAC AAAAAAAATT ATATATATTA TGTATGATAG 1081 GAGACGAATG GATTACCTAC CCTACTTTAT TTCTGAAAAA AACAAAAAAA AAACTAGTAA 1141 TTTCTTTTAA TACATCGTGT AGCGGTATTA GAACGCATAA ATATTACTAT TTATATGGCA 1201 ATTGATATAC GTGCACACAC ATGCAAAACT GATACTAAGG AAGTGTGATA TATGATAACC 1261 ACTATAGGAA TATTACTAAT ATTATTTCAA AATACTAAAT GTACTTTATC TTGCAATAAA 1321 TTTGCATATC CAATATTATT GTATATCATA GGTGTACATT ATTAATATAT GTTTCCCCTA 1381 TATTACTATT AATTAATTAT TTATTTATTT TTATCACTTA TTGCAAGTTT TCCTACATAT 1441 CACCTCTTAT AGCATATATT GCCAAAAAAA AATATTTTTT TAAATAACGT CAATATTTCT 1501 ATAAAAAATA TTATAAAAAT AATTTAATTT TTAAATACTT AAATAAAAAA AAAAATATAT 1561 TCTTAATTAA TTCTTAAACT TTTATAGTTA AAAATTTTCC ACGTTTTAAA AACAAAAACT 1621 CTCTAATTAA TTTAATTAAA TAATTTTATA ATTAAAATTA ATTAAAACTT TTATAATATT 1681 AAAATTGTTT ATCTCAAACA AATATAATAT TTTTAAAAAA ACACAAAAAT GgctagcGCA 1741 ATACTGATGA GTCCGTGAGG ACGAAACGAG TAAGCTCGTC TATTGCGAAA CTCAACAAAA 1801 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt 1861 ggcaccgagt cggtgctttt GGCCGGCATG GTCCCAGCCT CCTCGCTGGC GCCGGCTGGG 1921 CAACATGCTT CGGCATGGCG AATGGGACga ggagTGCATC CTGATGAGTC CGTGAGGACG 1981 AAACGAGTAA GCTCGTCGAT GCATGGATAG AAACAACgtt ttagagctag aaatagcaag 2041 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttGGC 2101 CGGCATGGTC CCAGCCTCCT CGCTGGCGCC GGCTGGGCAA CATGCTTCGG CATGGCGAAT 2161 GGGACgatga taccggtcat tatccgttca aaagaaattc ttattacata aatgcatgtt 2221 gtatataaat atgttgtata tacatgcata cctgttaata ctatttacaa gtttatggaa 2281 aattaattga cattttatat aagtttaatt attttaatag tctagcaaaa tgcttacatg 2341 aaataaatga acgacgtata tttatacata catgtgtatg tatgtatgta tgtaacaatg 2401 tttttgcttt tttttttttt tttttttttt gggcacataa atttacgtgc attttatgta 2461 cacacacata tgatatatat tgcattacat ctatttatat ttttttatgg attcacgtca 2521 tttatttttg agtaatattc tttacttgaa ttaataataa taataataat atatatggtg 2581 catattgtat gctatcattt tttctttttt atttcatttt atttttttac ttgaaaaaaa 2641 ataggccatt tcaatgaact atggttgaat tgtgtatata tatacatgca aacttaacat 2701 tttcattgat acaaattagc ataaaattgc cacgcgtgag tcgcttgtaa aaggcactct 2761 cgccatgcaa agatggttag catcaatttt tacaagcaca catgttaatg tgaaatgttt 2821 acatataata tatatatata tatatatata tatatatata tatatatata tatatatata 2881 tatatatata ataattaata tatatgtaat aattaatata tatgtaataa aatagcgtgt 2941 gtgccagttg ggggaggata tacccctttt ctagttttaa agcgatttgt atgctaaaat 3001 ataaagaatt tgaaaaggag aaaaataaaa tatgatccca tcctacatat ccctataatt 3061 tttatttagc ttgcattatt ttttctatta aatttatgtg taattaaaat agcggaataa 3121 tataatacaa taaaaaagtg cataaattgg aaatattttc ttgcattttc ttgcattttt 3181 ttttcaattt tttcccaatt ttttcccaat ttttttcaat tttttttcat tttttaaggg 3241 gtcatattaa atatatagaa taggattaca tattttctct acaattttta ttttatttta 3301 tatacacttt tccttttttt tgtcaaagtt ttaacaattc acacattcat ttgtatatat 3361 atatatatat catatcatat cgtatatttt atacacatag gtaaaaatta caaaATGact 3421 agtATGGACT ATAAGGACCA CGACGGAGAC TACAAGGATC ATGATATTGA TTACAAAGAC 3481 GATGACGATA AGATGGCCCC AAAGAAGAAG CGGAAGGTCG GTATCCACGG AGTCCCAGCA 3541 GCCGACAAGA AGTACAGCAT CGGCCTGGAC ATCGGCACCA ACTCTGTGGG CTGGGCCGTG 3601 ATCACCGACG AGTACAAGGT GCCCAGCAAG AAATTCAAGG TGCTGGGCAA CACCGACCGG 3661 CACAGCATCA AGAAGAACCT GATCGGAGCC CTGCTGTTCG ACAGCGGCGA AACAGCCGAG 3721 GCCACCCGGC TGAAGAGAAC CGCCAGAAGA AGATACACCA GACGGAAGAA CCGGATCTGC 3781 TATCTGCAAG AGATCTTCAG CAACGAGATG GCCAAGGTGG ACGACAGCTT CTTCCACAGA 3841 CTGGAAGAGT CCTTCCTGGT GGAAGAGGAT AAGAAGCACG AGCGGCACCC CATCTTCGGC 3901 AACATCGTGG ACGAGGTGGC CTACCACGAG AAGTACCCCA CCATCTACCA CCTGAGAAAG 3961 AAACTGGTGG ACAGCACCGA CAAGGCCGAC CTGCGGCTGA TCTATCTGGC CCTGGCCCAC 4021 ATGATCAAGT TCCGGGGCCA CTTCCTGATC GAGGGCGACC TGAACCCCGA CAACAGCGAC 4081 GTGGACAAGC TGTTCATCCA GCTGGTGCAG ACCTACAACC AGCTGTTCGA GGAAAACCCC 4141 ATCAACGCCA GCGGCGTGGA CGCCAAGGCC ATCCTGTCTG CCAGACTGAG CAAGAGCAGA 4201 CGGCTGGAAA ATCTGATCGC CCAGCTGCCC GGCGAGAAGA AGAATGGCCT GTTCGGAAAC 4261 CTGATTGCCC TGAGCCTGGG CCTGACCCCC AACTTCAAGA GCAACTTCGA CCTGGCCGAG 4321 GATGCCAAAC TGCAGCTGAG CAAGGACACC TACGACGACG ACCTGGACAA CCTGCTGGCC 4381 CAGATCGGCG ACCAGTACGC CGACCTGTTT CTGGCCGCCA AGAACCTGTC CGACGCCATC 4441 CTGCTGAGCG ACATCCTGAG AGTGAACACC GAGATCACCA AGGCCCCCCT GAGCGCCTCT 4501 ATGATCAAGA GATACGACGA GCACCACCAG GACCTGACCC TGCTGAAAGC TCTCGTGCGG 4561 CAGCAGCTGC CTGAGAAGTA CAAAGAGATT TTCTTCGACC AGAGCAAGAA CGGCTACGCC 4621 GGCTACATTG ACGGCGGAGC CAGCCAGGAA GAGTTCTACA AGTTCATCAA GCCCATCCTG 4681 GAAAAGATGG ACGGCACCGA GGAACTGCTC GTGAAGCTGA ACAGAGAGGA CCTGCTGCGG 4741 AAGCAGCGGA CCTTCGACAA CGGCAGCATC CCCCACCAGA TCCACCTGGG AGAGCTGCAC 4801 GCCATTCTGC GGCGGCAGGA AGATTTTTAC CCATTCCTGA AGGACAACCG GGAAAAGATC 4861 GAGAAGATCC TGACCTTCCG CATCCCCTAC TACGTGGGCC CTCTGGCCAG GGGAAACAGC 4921 AGATTCGCCT GGATGACCAG AAAGAGCGAG GAAACCATCA CCCCCTGGAA CTTCGAGGAA 4981 GTGGTGGACA AGGGCGCTTC CGCCCAGAGC TTCATCGAGC GGATGACCAA CTTCGATAAG 5041 AACCTGCCCA ACGAGAAGGT GCTGCCCAAG CACAGCCTGC TGTACGAGTA CTTCACCGTG 5101 TATAACGAGC TGACCAAAGT GAAATACGTG ACCGAGGGAA TGAGAAAGCC CGCCTTCCTG 5161 AGCGGCGAGC AGAAAAAGGC CATCGTGGAC CTGCTGTTCA AGACCAACCG GAAAGTGACC 5221 GTGAAGCAGC TGAAAGAGGA CTACTTCAAG AAAATCGAGT GCTTCGACTC CGTGGAAATC 5281 TCCGGCGTGG AAGATCGGTT CAACGCCTCC CTGGGCACAT ACCACGATCT GCTGAAAATT 5341 ATCAAGGACA AGGACTTCCT GGACAATGAG GAAAACGAGG ACATTCTGGA AGATATCGTG 5401 CTGACCCTGA CACTGTTTGA GGACAGAGAG ATGATCGAGG AACGGCTGAA AACCTATGCC 5461 CACCTGTTCG ACGACAAAGT GATGAAGCAG CTGAAGCGGC GGAGATACAC CGGCTGGGGC 5521 AGGCTGAGCC GGAAGCTGAT CAACGGCATC CGGGACAAGC AGTCCGGCAA GACAATCCTG 5581 GATTTCCTGA AGTCCGACGG CTTCGCCAAC AGAAACTTCA TGCAGCTGAT CCACGACGAC 5641 AGCCTGACCT TTAAAGAGGA CATCCAGAAA GCCCAGGTGT CCGGCCAGGG CGATAGCCTG 5701 CACGAGCACA TTGCCAATCT GGCCGGCAGC CCCGCCATTA AGAAGGGCAT CCTGCAGACA 5761 GTGAAGGTGG TGGACGAGCT CGTGAAAGTG ATGGGCCGGC ACAAGCCCGA GAACATCGTG 5821 ATCGAAATGG CCAGAGAGAA CCAGACCACC CAGAAGGGAC AGAAGAACAG CCGCGAGAGA 5881 ATGAAGCGGA TCGAAGAGGG CATCAAAGAG CTGGGCAGCC AGATCCTGAA AGAACACCCC 5941 GTGGAAAACA CCCAGCTGCA GAACGAGAAG CTGTACCTGT ACTACCTGCA GAATGGGCGG 6001 GATATGTACG TGGACCAGGA ACTGGACATC AACCGGCTGT CCGACTACGA TGTGGACCAT 6061 ATCGTGCCTC AGAGCTTTCT GAAGGACGAC TCCATCGACA ACAAGGTGCT GACCAGAAGC 6121 GACAAGAACC GGGGCAAGAG CGACAACGTG CCCTCCGAAG AGGTCGTGAA GAAGATGAAG 6181 AACTACTGGC GGCAGCTGCT GAACGCCAAG CTGATTACCC AGAGAAAGTT CGACAATCTG 6241 ACCAAGGCCG AGAGAGGCGG CCTGAGCGAA CTGGATAAGG CCGGCTTCAT CAAGAGACAG 6301 CTGGTGGAAA CCCGGCAGAT CACAAAGCAC GTGGCACAGA TCCTGGACTC CCGGATGAAC 6361 ACTAAGTACG ACGAGAATGA CAAGCTGATC CGGGAAGTGA AAGTGATCAC CCTGAAGTCC 6421 AAGCTGGTGT CCGATTTCCG GAAGGATTTC CAGTTTTACA AAGTGCGCGA GATCAACAAC 6481 TACCACCACG CCCACGACGC CTACCTGAAC GCCGTCGTGG GAACCGCCCT GATCAAAAAG 6541 TACCCTAAGC TGGAAAGCGA GTTCGTGTAC GGCGACTACA AGGTGTACGA CGTGCGGAAG 6601 ATGATCGCCA AGAGCGAGCA GGAAATCGGC AAGGCTACCG CCAAGTACTT CTTCTACAGC 6661 AACATCATGA ACTTTTTCAA GACCGAGATT ACCCTGGCCA ACGGCGAGAT CCGGAAGCGG 6721 CCTCTGATCG AGACAAACGG CGAAACCGGG GAGATCGTGT GGGATAAGGG CCGGGATTTT 6781 GCCACCGTGC GGAAAGTGCT GAGCATGCCC CAAGTGAATA TCGTGAAAAA GACCGAGGTG 6841 CAGACAGGCG GCTTCAGCAA AGAGTCTATC CTGCCCAAGA GGAACAGCGA TAAGCTGATC 6901 GCCAGAAAGA AGGACTGGGA CCCTAAGAAG TACGGCGGCT TCGACAGCCC CACCGTGGCC 6961 TATTCTGTGC TGGTGGTGGC CAAAGTGGAA AAGGGCAAGT CCAAGAAACT GAAGAGTGTG 7021 AAAGAGCTGC TGGGGATCAC CATCATGGAA AGAAGCAGCT TCGAGAAGAA TCCCATCGAC 7081 TTTCTGGAAG CCAAGGGCTA CAAAGAAGTG AAAAAGGACC TGATCATCAA GCTGCCTAAG 7141 TACTCCCTGT TCGAGCTGGA AAACGGCCGG AAGAGAATGC TGGCCTCTGC CGGCGAACTG 7201 CAGAAGGGAA ACGAACTGGC CCTGCCCTCC AAATATGTGA ACTTCCTGTA CCTGGCCAGC 7261 CACTATGAGA AGCTGAAGGG CTCCCCCGAG GATAATGAGC AGAAACAGCT GTTTGTGGAA 7321 CAGCACAAGC ACTACCTGGA CGAGATCATC GAGCAGATCA GCGAGTTCTC CAAGAGAGTG 7381 ATCCTGGCCG ACGCTAATCT GGACAAAGTG CTGTCCGCCT ACAACAAGCA CCGGGATAAG 7441 CCCATCAGAG AGCAGGCCGA GAATATCATC CACCTGTTTA CCCTGACCAA TCTGGGAGCC 7501 CCTGCCGCCT TCAAGTACTT TGACACCACC ATCGACCGGA AGAGGTACAC CAGCACCAAA 7561 GAGGTGCTGG ACGCCACCCT GATCCACCAG AGCATCACCG GCCTGTACGA GACACGGATC 7621 GACCTGTCTC AGCTGGGAGG CGACAAAAGG CCGGCGGCCA CGAAAAAGGC CGGCCAGGCA 7681 AAAAAGAAAA AGgctagtag taaaggagaa gaacttttca ctggagttgt cccAATTCTT 7741 GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT 7801 GATGCAACAT ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT 7861 CCATGGCCAA CACTTGTCAC TACTTTCGCG TATGGTCTTC AATGCTTTGC GAGATACCCA 7921 GATCATATGA AACAGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA 7981 AGAACTATAT TTTTCAAAGA TGACGGGAAC TACAAGACAC GTGCTGAAGT CAAGTTTGAA 8041 GGTGATACCC TTGTTAATAG AATCGAGTTA AAAGGTATTG ATTTTAAAGA AGATGGAAAC 8101 ATTCTTGGAC ACAAATTGGA ATACAACTAT AACTCACACA ATGTATACAT CATGGCAGAC 8161 AAACAAAAGA ATGGAATCAA AGTTAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC 8221 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA 8281 CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA 8341 GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA 8401 CTATACAAAT AAggatccGT TTTTCTTACT TATATATTTA TACCAATTGA TTGTATTTAT 8461 AACTGTAAAA ATGTGTATGT TGTGTGCATA TTTTTTTTTG TGCATGCACA TGCATGTAAA 8521 TAGCTAAAAT TATGAACATT TTATTTTTTG TTCAGAAAAA AAAAACTTTA CACACATAAA 8581 ATGGCTAGTA TGAATAGCCA TATTTTATAT AAATTAAATC CTATGAATTT ATGACCATAT 8641 TAAAAATTTA GATATTTATG GAACATAATA TGTTTGAAAC AATAAGACAA AATTATTATT 8701 ATTATTATTA TTTTTACTGT TATAATTATG TTGTCTCTTC AATGATTCAT AAATAGTTGG 8761 ACTTGATTTT TAAAATGTTT ATAATATGAT TAGCATAGTT AAATAAAAAA AGTTGAAAAA 8821 TTAAAAAAAA ACATATAAAC ACAAATGATG TTTTTTCCTT CAATTTcggc gcctgatgcg 8881 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac 8941 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc 9001 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 9061 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct 9121 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg 9181 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 9241 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 9301 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 9361 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 9421 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 9481 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 9541 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 9601 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 9661 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 9721 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 9781 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 9841 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 9901 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 9961 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 10021 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 10081 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 10141 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 10201 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 10261 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 10321 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 10381 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 10441 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 10501 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 10561 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 10621 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 10681 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag 10741 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 10801 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 10861 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 10921 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 10981 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 11041 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 11101 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 11161 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 11221 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 11281 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc 11341 caagcttgat aattcctgca gcccagctta attcttttcg agctctttat gcttaagttt 11401 acaatttaat attcatactt taagtatttt ttgtagtatc ctagatattg tgctttaaat 11461 gctcacccct caaagcacca gtaatatttt catccactga aataccatta aattttcaaa 11521 aaaatactat gcatataatg ttatacatat aaacataaaa cgccatgtaa atcaaaaaat 11581 atataaaaat atgtataaaa ataaatatgc actaaatata agctaattat gcataaaaat 11641 taaagtgccc tttattaact agctagtcgt aattatttat atttctatgt tataaaaaaa 11701 tcctcatata ataatataat taatatatgt aatgtttttt ttattttata attttaatat 11761 aaaataatat gtaaattaat tcaaaaaata aatataattg ttgtgaaaca aaaaacgtaa 11821 ttttttcatt tgccttcaaa atttaaattt attttaatat ttcctaaaat atatatactt 11881 tgtgtataaa tatataaaaa tatatatttg cttataaata aataaaaaat tttataaaac 11941 atagggggat ccatggttgg ttcgctaaac tgcatcgtcg ctgtgtccca gaacatgggc 12001 atcggcaaga acggggacct gccctggcca ccgctcagga acgaatttag atatttccag 12061 agaatgacca caacctcttc agtagaaggt aaacagaatc tggtgattat gggtaagaag 12121 acctggttct ccattcctga gaagaatcga cctttaaagg gtagaattaa tttagttctc 12181 agcagagaac tcaaggaacc tccacaagga gctcattttc tttccagaag tctagatgat 12241 gccttaaaac ttactgaaca accagaatta gcaaataaag tagacatggt ctggatagtt 12301 ggtggcagtt ctgtttataa ggaagccatg aatcacccag gccatcttaa actatttgtg 12361 acaaggatca tgcaagactt tgaaagtgac acgttttttc cagaaattga tttggagaaa 12421 tataaacttc tgccagaata cccaggtgtt ctctctgatg tccaggagga gaaaggcatt 12481 aagtacaaat ttgaagtata tgagaagaat gattaagtcg accttaatta tataatggtt 12541 tttacacttt tattatatta cgtgcattaa aagttttttt ttatatatca tattttatac 12601 ataaactttt tatttttttt caacaattaa aatgttgaat taagtatatt ttatatattt 12661 ttgtagttat tataataaat aatgcaaaca tatgtgaata tatataccta ttaaacacat 12721 taatatatat ataaagaaac gcacgttaat tgtaaagtat tcttatgaac tataaattaa 12781 ttttaatatt aatagcttca ttattattat ttttttttat tatagctagt aataatggtt 12841 aaaaatttaa ttatcaaatt tatttatttt taaataacaa aaataatgta tagaaagaaa 12901 aacatataaa caaatataag caatgcgaca atattttcat gagcattgtg c //