LOCUS       Exported               10417 bp ds-DNA     circular SYN 05-JUL-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   ICES
  TITLE     Direct Submission
  JOURNAL   Exported Jul 5, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..302
                     /label=P6
                     /label=P6 - BbsI
     misc_feature    303..3665
                     /label=sth1Cas9
     terminator      3830..3859
                     /label=fd-ter
     promoter        3860..4144
                     /label=P3
     misc_feature    complement(4174..4523)
                     /label=LacZ cassette
     misc_feature    4682..4731
                     /label=Term
     promoter        complement(4759..4855)
                     /label=KasOP
     misc_feature    4946..5534
                     /label=ori
     CDS             5738..6538
                     /codon_start=1
                     /label=aac
                     /label=aac(3)IV
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(7480..9438)
                     /codon_start=1
                     /label=rep
                     /label=rep (pSG5) - BbsI
                     /translation="MPGSTGPRNGTSPGLASRARRPRNWRSMPSEDVCSVTSVRENSAS
                     TPPRPAGPKCHCGTPIVHIPGKRPKIYCSDACRKRAKRLIADQSRSVASTTTAGNPDAG
                     FSSKGKSPSTAGQSAVTGTGSTPNEGAATAGREDPRDERFARRDRHQTVSLNEAFKGCG
                     TRLTAGTAELMWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLADAGYG
                     LHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGIIGYE
                     RAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFHDALAGAWAAGVESAGGYTVSKTC
                     DRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVSKIGLELAGQNF
                     KAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLNRLIK
                     ELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGLPGVR
                     RLVESWGLVCGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANEARTE
                     ELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKLAPVL
                     QPWGRLPGDCLRVDTAVA"
     misc_feature    9930..10417
                     /label=oriT
     misc_feature    10089
                     /label=oriT nicking site
ORIGIN
        1 gccctgcagg cggaagtcag gtagacacga cttccgctag tccttgcaag gtctgctgac
       61 gtgaggcggg gcggtcgttt ttgaccgccc tgccttcgtc atgtaggctc gctcgctgtg
      121 cctggcgtgt catcagacgc ccaggtcccg gtgccgtgag gcccgggcca tcgagccggt
      181 ggtacgtggc tgcggtcccc ttgtgagggc tgcgcgccgt gtgctgtccg gcgcgcacag
      241 ccttgaatcc acccgcgggg gccggccggt ctccgtgagc tcgagtagac gacggagacg
      301 taatgtcgga cctggtgctg ggcctggaca tcggcatcgg ctccgtcggc gtcggcatcc
      361 tgaacaaggt gaccggcgag atcatccaca agaactcccg catcttcccg gccgcacagg
      421 ccgagaacaa cctggtccgc cgcaccaacc gccagggccg ccgcctggcc cgccgcaaga
      481 agcaccgccg cgtccgcctg aaccgcctgt tcgaggagtc cggcctgatc accgacttca
      541 ccaagatcag catcaacctg aacccgtacc agctgcgcgt caagggcctg accgacgagc
      601 tgtccaacga ggagctgttc atcgccctga agaacatggt caagcaccgc ggcatctcct
      661 acctggacga cgcctccgac gacggcaact cctccgtcgg cgactacgcc cagatcgtca
      721 aggagaactc caagcagctg gaaaccaaga cccccggcca gatccagctg gagcgctacc
      781 agacctacgg ccagctgcgc ggcgacttca ccgtcgagaa ggacggcaag aagcaccgcc
      841 tgatcaacgt gttcccgacc tccgcctacc gctccgaggc cctgcgcatc ctccagaccc
      901 agcaggagtt caacccgcag atcaccgacg agttcatcaa ccgctacctg gagatcctga
      961 ccggcaagcg caagtactac cacggccccg gcaacgagaa gtcccgcacc gactacggcc
     1021 gctaccgcac ctccggcgag acgctggaca acatcttcgg catcctgatc ggcaagtgca
     1081 ccttctaccc ggacgagttc cgcgccgcca aggcctccta caccgcccag gagttcaacc
     1141 tgctgaacga cctgaacaac ctgaccgtcc cgaccgagac gaagaagctg tccaaggagc
     1201 agaagaacca gatcatcaac tacgtcaaga acgagaaggc gatgggcccg gccaagctgt
     1261 tcaagtacat cgccaagctg ctgtcctgcg acgtcgccga catcaagggc taccgcatcg
     1321 acaagtccgg caaggccgag atccacacct tcgaggccta ccgcaagatg aaaaccctgg
     1381 agacgctgga catcgagcag atggaccgcg agacgctgga caagctggcc tacgtcctga
     1441 ccctgaacac cgagcgggag ggcatccagg aagccctgga gcacgagttc gccgacggct
     1501 ccttctccca gaagcaggtg gacgagctgg tccagttccg caaggccaac tcctccatct
     1561 tcggcaaggg ctggcacaac ttctccgtca agctgatgat ggagctgatc ccggagctgt
     1621 acgagacgtc cgaggagcag atgaccatcc tgacccgcct gggcaagcaa aagaccacct
     1681 cctcctccaa caagaccaag tacatcgacg agaagctgct gaccgaggaa atctacaacc
     1741 cggtcgtcgc caagtccgtc cgccaggcca tcaagatcgt caacgccgcc atcaaggagt
     1801 acggcgactt cgacaacatc gtcatcgaga tggcccgcga gacgaacgag gacgacgaga
     1861 agaaggccat ccagaagatc cagaaggcca acaaggacga gaaggacgcc gccatgctga
     1921 aggccgccaa ccagtacaac ggcaaggccg agctgccgca ctccgtgttc cacggccaca
     1981 agcagctggc caccaagatc cggctgtggc accagcaggg cgagcgctgc ctgtacaccg
     2041 gcaagaccat ctccatccac gacctgatca acaactccaa ccagttcgag gtggaccaca
     2101 tcctgccgct gtccatcacc ttcgacgact ccctggccaa caaggtcctg gtctacgcca
     2161 ccgccaacca ggagaagggc cagcgcaccc cgtaccaggc cctggactcg atggacgacg
     2221 cctggtcctt ccgcgagctg aaggccttcg tccgcgagtc caagaccctg tccaacaaga
     2281 agaaggagta cctgctgacc gaagaggaca tctccaagtt cgacgtccgc aagaagttca
     2341 tcgagcgcaa cctggtggac acccggtacg cctcccgcgt cgtcctgaac gccctccagg
     2401 agcacttccg cgcccacaag atcgacacca aggtgtccgt cgtccgcggc cagttcacct
     2461 cccagctccg ccgccactgg ggcatcgaaa agacccgcga cacctaccac caccacgccg
     2521 tggacgccct gatcatcgcc gcctcctccc agctgaacct gtggaagaag cagaagaaca
     2581 ccctggtgtc ctactccgag gaccagctgc tggacatcga gacgggcgag ctgatctccg
     2641 acgacgagta caaggagtcc gtgttcaagg ccccgtacca gcacttcgtg gacaccctga
     2701 agtccaagga gttcgaggac tccatcctgt tctcctacca ggtggactcc aagttcaacc
     2761 gcaagatcag cgacgccacc atctacgcca cccgccaggc caaggtcggc aaggacaagg
     2821 ccgacgagac gtacgtcctg ggcaagatca aggacatcta cacccaggac ggctacgacg
     2881 ccttcatgaa aatctacaag aaggacaagt ccaagttcct gatgtaccgc cacgacccgc
     2941 agaccttcga gaaggtcatc gagccgatcc tggagaacta cccgaacaag cagatcaacg
     3001 acaagggcaa ggaagtcccg tgcaacccgt tcctgaagta caaggaagag cacggctaca
     3061 tccgcaagta ctccaagaag ggcaacggcc cggagatcaa gtccctgaag tactacgact
     3121 ccaagctggg caaccacatc gacatcaccc cgaaggactc caacaacaag gtcgtcctcc
     3181 agtccgtctc cccgtggcgc gccgacgtct acttcaacaa gaccaccggc aagtacgaga
     3241 tcctgggcct gaagtacgcc gacctccagt tcgacaaggg caccggcacc tacaagatca
     3301 gccaggagaa gtacaacgac atcaagaaga aggaaggcgt ggactccgac tccgagttca
     3361 agttcaccct gtacaagaac gacctgctgc tggtcaagga caccgagacg aaggagcagc
     3421 agctgttccg cttcctgtcc cgcaccatgc cgaagcagaa gcactacgtc gagctgaagc
     3481 cgtacgacaa gcagaagttc gagggcggcg aggccctgat caaggtcctg ggcaacgtcg
     3541 ccaactccgg ccagtgcaag aagggcctgg gcaagtccaa catctccatc tacaaggtcc
     3601 gcaccgacgt cctgggcaac cagcacatca tcaagaacga gggcgacaag ccgaagctcg
     3661 acttccagat ctacgcgttc cccgcaaaag cggcctttga ctccctgcaa gcctcagcga
     3721 ccgaatatat cggttatgcg tgggcgatgg ttgttgtcat tgtcggcgca actatcggta
     3781 tcaagctgtt taagaaattc acctcgaaag caagctgata aaccgataca attaaaggct
     3841 ccttttggag cctttttttg ctgctccttc ggtcggacgt gcgtctacgg gcaccttacc
     3901 gcagccgtcg gctgtgcgac acggacggat cgggcgaact ggccgatgct gggagaagcg
     3961 cgctgctgta cggcgcgcac cgggtgcgga gcccctcggc gagcggtgtg aaacttctgt
     4021 gaatggcctg ttcggttgct ttttttatac ggctgccaga taaggcttgc agcatctggg
     4081 cggctaccgc tatgatcggg gcgttcctgc aattcttagt gcgagtatct gaaaggggat
     4141 acgcaagtct tctcagccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     4201 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     4261 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     4321 gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta ccgggccccc
     4381 cctcgaggtc ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     4441 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4501 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4561 ttgcgttgcg ctcactgccc gctttccacc ggtgaagaca tgtttttgta ctcgaaagaa
     4621 gctacaaaga taaggcttca tgccgaaatc aacaccctgt cattttatgg cagggtgttt
     4681 tactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt atctagagct
     4741 agcatgcata tgaagctttg ttcacattcg aacggtctct gctttgacaa catgctgtgc
     4801 ggtgttgtaa agtcgtggcc aggagaatac gacagcgtgc aggactgggg gagttatgac
     4861 tagtgtgaaa agtcagtatc cagtcgtgta gttcttatta ccttctagag gccaggaacc
     4921 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4981 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5041 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5101 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5161 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5221 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5281 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5341 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     5401 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5461 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5521 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5581 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5641 ttttggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca ccaccgacta
     5701 tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa
     5761 tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca accttggggt
     5821 tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc ccctcgaaga
     5881 tgggccactt ggactgatcg aggccctgcg tgctgcgctg ggtccgggag ggacgctcgt
     5941 catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt cgcccgttac
     6001 accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc
     6061 ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct ctgatccatt
     6121 gcccctgcca cctcactcgc ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca
     6181 ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg ccgagttgat
     6241 ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg gcaagttggt
     6301 acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg cggacaggtg
     6361 gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg ctcggttgat
     6421 ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc cgttgatctt
     6481 cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt cgattggctg
     6541 agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga ttgctggggc
     6601 aacacgtgga gcggatcggg gattgtcttt cttcagctcg ctgatgatat gctgacgctc
     6661 aatgccgttt ggcctccgac taacgaaaat cccgcatttg gacggctgat ccgattggca
     6721 cggcggacgg cgaatggcgg agcagacgct cgtccggggg caatgagata tgaaaaagcc
     6781 tgaactcacc gcgacgtatc gatgtcgagg ttcctcaggg gagccacccc agagaagccc
     6841 tcggagctga gcggagctat ttccaaagcc atgccagcta gagacagtgc acaccgccaa
     6901 gcatctgcaa aaccctcgcc tggagggaaa gtgcaatgta cgtacgctgg tttccctcca
     6961 gaaagggatt ctgcggcttc tcaactttgg aagaagaggc ggaacgaact gctgtttcag
     7021 cctactcatg tgaggaggct ggtcctttac cctgatgccc gagggcatca gggtaaagga
     7081 ccagcctcgc caactaggag cgctaccaag gcgaacacaa cccaggactt ggtagaaccc
     7141 ttcgccggag aagttcagac tcatcggcat gagcacccct cgccgcgcac tcggcaccgg
     7201 tccgtcgacc accaccagga cgtcgttgtc gacgtcggcc ccgcggctcc tgcccgccga
     7261 acgcgtcgtc gtcgacggcc tggtgctcat cgacgagcac ccggagccag gtgaaaagcg
     7321 ccggcggacg ctcggactgg gcgcgggatt ccagcagtaa cccaggtccg ccggccacct
     7381 cacggcaggc agaccctcgg ctttcgcgcc gggaccgcca tgagaccgcc acccggatgt
     7441 ccggggtggc ggtctcatgg cggtccctca gcggccctac gcgaccgctg tgtcgacgcg
     7501 gaggcagtct ccggggagcc ggccccaggg ctggagtacc ggggcgagct tgcccttgca
     7561 gcggcggcac acgggcgacg cggcgccggg cgggggagtc ttgacgtcga cggtgaccgg
     7621 cttcggcttc aggcgcttcc gaaggctgat cgtcgggcgg atcgcctcct tcttcggctg
     7681 acgcactcgg tcgagcgaag cggccagctc ctccgtgcgg gcctcgttgg ccttccggcg
     7741 cgcctcgcgg aaagcagctt cctcctcgga catgacctcg aacctcatct ggtcagcgtc
     7801 aaggtcgccc ggcgcggccg gcgcttccgg ggcgggcggg tccaggacgt ccttgccaca
     7861 caccaggccc caggactcga cgagccgccg gacgcccggt aggccgtacg tctcggcgac
     7921 cttgatgagg tcgaggcgac gtccggcgac gcgggcgatg tatcggtacc agatgtaggc
     7981 cgggatgacc gcgatggcga ccaggccctc ggtgtcgtcg gtgatctcct cctcggtgcg
     8041 gacgtcctgc tggatgccga gttccttgat cagccggttc aggttctgcg accggtagtg
     8101 cttgcggacc tggaacacgc cgaactcgcg ctcgcggtac ttctcgacga acgggccggg
     8161 ccgacgaagc cgctgcagct cggcggccgc cgcgtcgccc aggtcgagcg gtcccatgcg
     8221 gtcgtcgccg cgaccggcct tgaagttctg tccggccagc tccaggccga tcttggagac
     8281 gccgcccttg gtcttgtcgc cgtccttgta gaggtagcgg gcctgcttgc ccgcatcgcc
     8341 gtcagcggcg tccgcgccgt tgagtgggcg cacgtcggtg ccgtggccct tgccctcaca
     8401 ggagcaaccg ggccggtcgc acgtcttgct gacggtgtag ccgcccgcgg attcgacccc
     8461 ggcggcccag gctccggcga gtgcgtcgtg gaacgcggcc tgggcgtccg ggccgagcac
     8521 ctcgcgggtg acccagagcg tgtgccagtg caggtgccag ccggagcccc agccgaaggt
     8581 gtcctcgaag gcccgctcgt agccgatgat cccgaagtcg tcgcgcatcg tgcgccagcg
     8641 gcggccggac gagccgtacg cgcccttcca gccgtcgtgc aagaccgcga ccaggccgtg
     8701 ccgcattccc ttgcggacgg tgccgaacgc catgcgctcg aagtggcgca acgtgttcgt
     8761 gccaaggtgc agcccgtacc cggcgtccgc gagaccgtcg gcggcgagct gcacgttcga
     8821 gccccgtacg gccaggatgc ggctcatgca ccacgggcag gtgtggacgt tgttgcagcg
     8881 gcacgtgttg ccccacgtcg cctcgcccgg cttccacatc agctcggccg tcccggcagt
     8941 gagccgggtc ccgcagccct tgaacgcctc gttcagcgac accgtctggt gccggtcccg
     9001 ccgggcgaac cgctcgtcgc gcgggtcctc ccgcccggct gtcgcggcac cctcgtttgg
     9061 ggtagaaccc gttccagtta cagcgctctg acctgcagtg gacggagatt ttcccttact
     9121 actaaagccc gcgtccggat tacccgctgt agtcgtgctt gctacgctgc gtgactggtc
     9181 cgcaatgaga cgctttgcgc gctttcggca ggcgtccgag cagtagattt tggggcgctt
     9241 cccggggatg tggacgatcg gggtgccgca gtggcacttc ggtccggcgg ggcgcggtgg
     9301 tgtcgacgcg ctgttctctc gtacgctcgt cacagagcaa acgtcctcac tcggcatgct
     9361 gcgccagttg cgggggcggc gagcccggga ggccaatccc gggctcgtgc catttctggg
     9421 tcctgttgat cctggcattg gtgtggccgt tcattgcccc tgctcgctcc tgacgcgccg
     9481 atagacgtcc gatacgcccg gtgctggtgg gatttgatag gtcggaagaa gccccgcccg
     9541 ggcctgggcg gggcttcctg tgcgtcagga cctcctcgtc gtgagcctct tcggcctatg
     9601 gacggagtga cctcgtgatc cgttacagcc gcgcgcgctc gcgtagagcg gtctcatcag
     9661 ttccacgaac ggtcctcttc gcagatcagg gcgttggggc ggagtctcac caaggactac
     9721 gtctgctggc gatttccgtt acaccccggg cggtggccgg cgcacacgcg cgcccgcgtt
     9781 gggcagtgca gaaagtgcag aaacctaggc gctgatggtc caggtccacg gttcgtcgtc
     9841 ggcggcggcg cgggcggcgg cgtcggccag ggcgcgggcg agaccggcta cggcgggctt
     9901 gatgcgccgg ttgcgggcga ccttgagcag ctagtatgca ggtcgacgga tcttttccgc
     9961 tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc
    10021 acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg
    10081 tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt
    10141 cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggctac
    10201 cgccggcgta acagatgagg gcaagcggat ggctgatgaa accaagccaa ccaggaaggg
    10261 cagcccacct atcaaggtgt actgccttcc agacgaacga agagcgattg aggaaaaggc
    10321 ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc gtcggccagg gctacaaaat
    10381 cacgggcgtc gtggactatg agcacgtccg cgagctg
//
