LOCUS       NmMetQ_(with_pelB_s.s.) 6222 bp ds-DNA     circular     09-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     Cleavage Site     2872..2886
                       /label="Enterokinase "
     rep_origin        3801..4107
                       /label="F1 ori"
     rep_origin        3784..4239
                       /label="M13 origin"
     primer            complement(3686..3704)
                       /label="T7 Reverse"
     misc_binding      2686..2708
                       /label="LacO"
     CDS               4569..5228
                       /label="AmpR"
     Sequencing Primer complement(2985..3009)
                       /label="NmMetQ-Rev1"
     misc_feature      2890..3630
                       /label="NmMetQ"
     rep_origin        5326..6008
                       /label="ColE1 origin"
     CDS               2827..2856
                       /label="10x His Tag"
     misc_feature      2755..3630
                       /label="pelB-10xHis-EK-noSS-MetQ"
     Signal Sequence   2755..2826
                       /label="pelB"
     Sequencing Primer 3455..3479
                       /label="NmMetQ-For1"
     primer_bind       2667..2686
                       /label="T7 Forward"
ORIGIN
        1 gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
       61 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
      121 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
      181 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
      241 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
      301 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
      361 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
      421 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
      481 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
      541 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
      601 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
      661 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
      721 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca
      781 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg
      841 atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt
      901 ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca
      961 agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag
     1021 agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg
     1081 acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
     1141 atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca
     1201 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1261 gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac
     1321 gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac
     1381 gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca
     1441 tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc
     1501 ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc
     1561 agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact
     1621 ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca
     1681 gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg
     1741 ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa
     1801 aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt
     1861 gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc
     1921 actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg
     1981 ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc
     2041 gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga
     2101 ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc
     2161 cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga
     2221 aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac
     2281 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
     2341 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
     2401 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
     2461 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
     2521 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
     2581 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
     2641 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
     2701 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat acatatgaaa
     2761 tacctgctgc cgaccgctgc tgctggtctg ctgctcctcg ctgcccagcc ggcgatggcc
     2821 atgggccatc atcatcatca tcatcatcat catcacagca gcggccatat cgacgacgac
     2881 gacaagcatA TGAAAGAAAT CGTCTTCGGC ACGACCGTCG GCGACTTCGG CGATATGGTC
     2941 AAAGAACAAA TCCAAGCCGA GCTGGAGAAA AAGGGCTACA CCGTCAAACT GGTCGAGTTT
     3001 ACCGACTATG TACGCCCGAA TCTGGCATTG GCTGAGGGCG AGTTGGACAT CAACGTCTTC
     3061 CAACACAAAC CCTATCTTGA CGACTTCAAA AAAGAACACA ATCTGGACAT CACCGAAGTC
     3121 TTCCAAGTGC CGACCGCGCC TTTGGGACTG TACCCGGGCA AGCTGAAATC GCTGGAAGAA
     3181 GTCAAAGACG GCAGCACCGT ATCCGCGCCC AACGACCCGT CCAACTTCGC CCGCGTCTTG
     3241 GTGATGCTCG ACGAACTGGG TTGGATCAAA CTCAAAGACG GCATCAATCC GTTGACCGCA
     3301 TCCAAAGCGG ACATCGCCGA GAACCTGAAA AACATCAAAA TCGTCGAGCT TGAAGCCGCG
     3361 CAACTGCCGC GTAGCCGCGC CGACGTGGAT TTTGCCGTCG TCAACGGCAA CTACGCCATA
     3421 AGCAGCGGCA TGAAGCTGAC CGAAGCCCTG TTCCAAGAAC CGAGCTTTGC CTATGTCAAC
     3481 TGGTCTGCCG TCAAAACCGC CGACAAAGAC AGCCAATGGC TTAAAGACGT AACCGAgGCC
     3541 TATAACTCCG ACGCGTTCAA AGCCTAcGCG CACAAACGCT TCGAGGGCTA CAAATCCCCT
     3601 GCCGCATGGA ATGAAGGCGC AGCCAAATAA ctcgaggatc cggctgctaa caaagcccga
     3661 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     3721 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
     3781 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3841 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3901 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3961 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4021 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4081 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4141 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4201 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc aggtggcact tttcggggaa
     4261 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4321 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4381 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4441 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4501 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4561 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4621 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4681 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4741 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4801 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4861 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     4921 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4981 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5041 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5101 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5161 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5221 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5281 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5341 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5401 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5461 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5521 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     5581 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5641 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5701 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5761 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5821 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5881 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5941 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6001 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6061 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6121 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     6181 tgcggtattt tctccttacg catctgtgcg gtatttcaca cc
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