LOCUS GGA_CrCas9_p8UASTEF-EY 12713 bp ds-DNA circular 22-SEP-2020 DEFINITION . FEATURES Location/Qualifiers misc_feature 3696..3773 /label="sgRNA" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 7..10 /label="Geneious type: ligation" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc CDS complement(1025..1885) /label="ampR (rev) CDS" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 6994..8295 /label="p8UASxpr-Tef" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 2125..2128 /label="Geneious type: ligation" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 3507..3531 /label="misc_feature" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 stem_loop complement(2097..2116) /label="stem_loop" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 6..10 /label="PstI misc_feature" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 2600..2799 /label="Geneious type: promoter prokaryote" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 2130..2134 /label="Bbsi misc_feature" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 2151..2568 /label="Promotor_SCR1-tRNA" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 6151..6278 /label="tLIP2" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 11..11 /label="misc_feature" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 12459..12704 /label="CYC-t" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 8305..12444 /label="Codon opt CAS 9 SV40" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 2497..2568 /label="tRNA-Gly" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 12..83 /label="E. coli his operon terminator misc_feature" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer_bind complement(147..166) /label="primer_bind" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 2151..2569 /label="sgRNA" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc CDS 2826..3500 /label="mrfp1 CDS" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer_bind complement(1147..1178) /label="primer_bind" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc CDS complement(251..865) /label="rep(pMB1) (rev) CDS" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind 1147..1178 /label="primer_bind" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer_bind 2008..2027 /label="primer_bind" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 6286..6711 /label="CloningVector" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 stem_loop 20..54 /label="stem_loop" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 12421..12441 /label="SV40 NLS" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 3862..3975 /label="Yl-ARS1" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 2136..2136 /label="Geneious type: Editing History Insertion" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 2594..2597 /label="Geneious type: ligation" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 4389..6146 /label="EYK" /ApEinfo_revcolor=#3366ff /ApEinfo_fwdcolor=#3366ff misc_feature 6712..6938 /label="ARS-Cen1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(2084..2087) /label="end of terminator misc_feature" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 2125..2129 /label="EcoRI misc_feature" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 7020..7128 /label="1UAS" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 2808..2819 /label="Geneious type: RBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 2594..2599 /label="Geneious type: motif" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 2134..2135 /label="Geneious type: Editing History Deletion" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 1164..1164 /label="Geneious type: Editing History Replacement" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 2594..3668 /label="RFP" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 3540..3668 /label="Geneious type: Terminator" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 4007..4379 /label="pTEF" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 266..266 /label="ORI misc_feature" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(5148..5169) /label="ForT7Endon Test Rv_ EYK" /note="sequence: CCTTGAACTTTACGAAGGATCG" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 2143..2160 /label="Promoter PolIII_GGP_A_Fw" /note="sequence: gggGGTCTCtGCCTcctgacgtCCCCAGTTGC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer complement(8633..8654) /label="Cas9_1_Rv" /note="sequence: GGGTGTCGCTCGTGCTTCTTGT" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer 11121..11145 /label="Cas9_6_Fw" /note="sequence: GAACACCAAGTACGACGAGAACGAC" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(3966..3988) /label="DRSfinal_ARS_GGP_B_Rv" /note="sequence: GGGGGTCTCtACCTAGCGGCCGCATACTACTG" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer complement(3648..3685) /label="RFP_SfiI_Rv" /note="sequence: ccGGCCCAGATGGCCtatataaacgcagaaaggcccac" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer 2579..2614 /label="RFP_SfiI_Fw" /note="sequence: ccGGCCATCTGGGCCctagagcaatacgcaaaccgc" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer 8305..8331 /label="Cas9_1_Fw" /note="sequence: ATGGATAAGAAATACTCCATTGGCCTG" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(2548..2579) /label="promoter PolIII_BsmbI Sfi Avr_Rv" /note="sequence: gggCCTAGGGGCCCAGATGGCCCGTCTCtCGAACCGGGGGCCCATCGATG" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(2548..2575) /label="Promoter PolIII_BsmbI_Rv" /note="sequence: CGTCTCtCGAACCGGGGGCCCATCGATG" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(3749..3767) /label="Verif sgRNA_Rv" /note="sequence: AAGCACCGACTCGGTGCCA" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer 2137..2156 /label="Promoter PolIII_GGP_O_Fw" /note="sequence: GGGGGTCTCtCTGACCTgacgtCCCCAG" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer 6913..6938 /label="ARS-Cen1_Verif_Fw" /note="sequence: GATGGCATCCCTAAATTTGATGAAAG" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer complement(3966..3987) /label="DRSfinal_ARS_GGP_M_Rv" /note="sequence: GGGGGTCTCtCGCAACCTAGCGGCCGCATACTACTG" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer 2494..2518 /label="Verif sgRNA_Fw" /note="sequence: CTTtGAAAAATACCTCTAATGCGCC" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer 11681..11702 /label="Cas9_7_Fw" /note="sequence: GGGACCCCAAGAAGTACGGAGG" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 4402..4423 /label="ForT7Endon test Fw_EYK" /note="sequence: ATCTGTTCAACGAGACTGACGA" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 ORIGIN 1 tcttcctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa 61 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc 121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 241 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa 301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 661 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 901 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 961 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 1021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 1081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 1141 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata 1201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 1261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 1321 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 1381 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 1441 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 1501 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 1561 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 1621 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 1681 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 1741 tccagttcga tataacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 1801 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 1861 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 1921 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 1981 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 2041 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta 2101 gctttcgcta aggatgattt ctggaattcg aagagcctCT GACCTgacgt CCCCAGTTGC 2161 AAAAGTTGAC ACAACTCTAG ATCTGCTTCC AAATATAGAA TCATAACAAG GGTTAGGGTG 2221 TGATTATATA ATATTGGTCT TAATTGATGT GCTAGGGCTT TAAAAGTTGG TTAAAATAAC 2281 GCTCTAATGC CTTTTTAATA TATTGTCTTT TTCAAAATCT CAAATCGGAC ACTTCTTCGT 2341 GTATGAGACT CCATTTTTTG GCTCCGTCAC GTGATATGTA TTATCAGCTA TAGTGGTGTA 2401 AACAAAGTTT TTTACTAGCT GTAATGGCAT TTTGTCGGAG TGGTAAATCG CCTTCTTGTT 2461 GTGCGTTCGA GTTCTGGACT CTGCACTGGG CTACTTtGAA AAATACCTCT AATGCGCCGA 2521 TGGTTTAGTG GTAAAATCCA TCGTTGCCAT CGATGGGCCC CCGGTTCGaG AGACGgggcc 2581 GGCCATCTGG GCCctagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 2641 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 2701 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 2761 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat 2821 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg 2881 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg 2941 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg 3001 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca 3061 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact 3121 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca 3181 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa 3241 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag 3301 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa 3361 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac 3421 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag 3481 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc 3541 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3601 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 3661 cgtttatata GGCCATCTGG GCCgggggCG TCTCtGAGCT AGAAATAGCA AGTTAAAATA 3721 AGGCTAGTCC GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TTTacgtcTA 3781 AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCAGATC 3841 CTCTAGAGTC GAAGCGGCCG CTATGTCTGA TAAAAGGATG TAACATAGGC AAGCTGCTCG 3901 TGAGTGTTGA GTACGAACCT TAGATCCAAA TCACCCGCAC CCACGGATAT ACTTGCTTGA 3961 ATATACAGTA GTATGCGGCC GCTAGGTCTG TTATCCCTAG ATCGATATAG AGacTgggtt 4021 ggcggcgcat ttgtgtccca aaaaacagcc ccaattgccc caattgaccc caaattgacc 4081 cagtagcgga cccaaccccg gcgagagccc ccttcacccc acatatcaaa cctcccccgg 4141 ttcccacact tgccgttaag ggcgtagggt actgcagtct ggaatctacg cttgttcaga 4201 ctttgtacta gtttctttgt ctggccatcc gggtaaccca tgccggacgc aaaatagact 4261 actgaaaatt tttttgcttt gtggttggga ctttagccaa gggtataaaa gaccaccgtc 4321 cccgaattac ctttcctctt cttttctctc tctccttgtc aactcacacc cgaaggatcg 4381 GATCCACAAT GTCCACAAAA CATCTGTTCA ACGAGACTGA CGAGCTCGTG CTCAAGTCTC 4441 TCGAGGGAGT TCAGGCTTCC CGATCAGCGT CCATTCTCTC CCACAGATTC AAGGTGCTCT 4501 ACAACGGAAC CCATTCCGCT GACCGAGTTG CAGTTCTTTC TGGAGGAGGC TCTGGCCACG 4561 AGCCTGCCCA CGCCGGCTTT GTGGGAGACA ACATGCTGAC CGGTGCCATC TGTGGCCCCG 4621 TCTTCGCGTC CCCTTCAGCC AAGCAGGTCG AGGCCGGCTG CAAGCTCGTG CCCTCCGACA 4681 AGGGTCATAT TCTGGTCGTC ACCAACTACA CCGGCGACAT GTTGCATTTT GGCCTCGCGG 4741 CCGAGAAGCT CAAGTCTCAG GGCCACAAGG TCGGCATCAT TAAGTCTGCC GACGATGTGG 4801 CTGTCGACCG AAAGAGCGGT GGTCTGGTCG GTCGACGAGG ACTGGCTGGA ACTGTGCTTC 4861 TGGACAAGAT TGTCGGAGGA GCTGCCTGGG ACAAGCTGTC CTTTGATGAA TGCATGGCCA 4921 TTGGAACCGA GGTGGCCGAG AACACCGCAA CCGCCTCAAT TGGACTGGAC TACTGCCACG 4981 TTCCTGGTCG ATCCGTTGAG AACCACGTGT CGCTCGACCA GAACGAGTGT CAATTCGGCC 5041 TGGGTATTCA CAATGAGCCC GGAGTCAAGA CCATCAACCC CGTGCCTGCC CCTGAGTCCA 5101 TGGTTGATAC TCTGCTCAAG TACCTAGTTT CGCAGGACGA CCCCGAGCGA TCCTTCGTAA 5161 AGTTCAAGGA GGGCGACGAG GTCATTCTCC TGGCCAACAA CCTCGGTGGA ATTTCCACCA 5221 TTGAGATGCG GGCTGCTGTC CAGCTTGCCC GAGAGCAACT CGAGAAGACC CACAAGATCA 5281 AGTCTGTGCG AGTTCTGTGC GGCACCTTCA TGAGCTCTCT CAACGCTCCC GGCTTCTCTA 5341 TCACCCTGGT GAACCTGTCT AACGGCTCCC ACAGCAAGAA CGTGCTCAAG TACCTGGACG 5401 CTGTGTCCGA CGCCCCTGCC TGGGTCAATG TGGCCCCTCC TACCTCGGTC AAGCCCTTTA 5461 TCAACGAGGA CAAGATCTTC GACGACGAGA CTTCCAACAT TAAGGCTCCC ACACTGGACA 5521 TTCCTGAACA GACCGTGGTT GCTGCTCTCA CTCAGGCCTC CCAGAACATT ATCAAGGCCG 5581 AGCCTCAGCT TACTGCATGG GATACCGAAA TGGGAGATGG AGACTGTGGA CACACCATTG 5641 AACATGGCTG CCGAGCTCTT CTCGAGTACC TCAACAAGAA CAAGAGCGAC CCAAAGGCTC 5701 TCGAGATCAT CCCCATTGTG CGGGCTGTCG TTCACATCAC CGAGGAGGAT ATGGGAGGCA 5761 CCCTGGGAGC TATCTTCGGC ATTTTTTTTG CCTCTTTCCT TAACGCTCTT CTGCTGGACC 5821 CTCTGTCCCA TAAGACTGAT GTCAATGTGA CTGATAAGCT CGTCAACGCT GCTAACACCG 5881 GTCTGGAAAG TCTCATGAAC CACACTCCTG CTCGACCTGG TGACCGAACT GTCATGGACG 5941 TGTTGATCCC CTACGTGCAG TCTCTGGTGT CCACCAAGGA TATCAAGGAG GCCGCTCTCA 6001 AGGCCAAGCA GGCTGCCGAG GGCACCAAGA AGATCAAGCC TCGTCTTGGA CGAGCAGTCT 6061 ATGTTGGGGA GAAGGACGGT GAGCTTCCTC CCGACCCTGG AGCTTGGGCT GTCTACGAGC 6121 TGGTTGATGG TTTTGCCAAC CACAAGTAGC ctagggtgtc tgtggtatct aagctattta 6181 tcactcttta caacttctac ctcaactatc tactttaata aatgaatatc gtttattctc 6241 tatgattact gtatatgcgt tcctctaaga caaatcgaat GACGGGGCCC TTTCGTCaCG 6301 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGtG ACGGTCACAG 6361 CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG 6421 GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC 6481 ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT 6541 CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC 6601 GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT 6661 CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTCGA GCTCGGTACC CGCTTTTCGT 6721 AGATAATGGA ATACAAATGG ATATCCAGAG TATACACATG GATAGTATAC ACTGACACGA 6781 CAATTCTGTA TCTCTTTATG TTAACTACTG TGAGGCGTTA AATAGAGCTT GATATATAAA 6841 ATGTTACATT TCACAGTCTG AACTTTTGCA GATTACCTAA TTTGGTAAGA TATTAATTAT 6901 GAACTGAAAG TTGATGGCAT CCCTAAATTT GATGAAAGGG GGAaCCCCCG GGTTCGAAGG 6961 TACCAAGGAA GCATGCGGTA CCCGAATTCG CTTCGATACG CGTatcgatg atacgcgtcc 7021 atgCtgaggt gtctcacaag tgccgtgcag tcccgccccc acttgcttct ctttgtgtgt 7081 agtgtacgta cattatcgag agggttgttc ccgcccacct cgatccggca tgctgaggtg 7141 tctcacaagt gccgtgcagt cccgccccca cttgcttctc tttgtgtgta gtgtacgtac 7201 attatcgaga gggttgttcc cgcccacctc gatccggcat gctgaggtgt ctcacaagtg 7261 ccgtgcagtc ccgcccccac ttgcttctct ttgtgtgtag tgtacgtaca ttatcgagag 7321 ggttgttccc gcccacctcg atccggcatg ctgaggtgtc tcacaagtgc cgtgcagtcc 7381 cgcccccact tgcttctctt tgtgtgtagt gtacgtacat tatcgagagg gttgttcccg 7441 cccacctcga tccgGcatgC tgaggtgtct cacaagtgcc gtgcagtccc gcccccactt 7501 gcttctcttt gtgtgtagtg tacgtacatt atcgagaggg ttgttcccgc ccacctcgat 7561 ccggcatgct gaggtgtctc acaagtgccg tgcagtcccg cccccacttg cttctctttg 7621 tgtgtagtgt acgtacatta tcgagagggt tgttcccgcc cacctcgatc cggcatgctg 7681 aggtgtctca caagtgccgt gcagtcccgc ccccacttgc ttctctttgt gtgtagtgta 7741 cgtacattat cgagagggtt gttcccgccc acctcgatcc ggcatgctga ggtgtctcac 7801 aagtgccgtg cagtcccgcc cccacttgct tctctttgtg tgtagtgtac gtacattatc 7861 gagagggttg ttcccgccca cctcgatccg gcatgcactg atcacgggca aaagtgcttc 7921 gatagagagg gggttggcgg cgcatttgtg tcccaaaaaa cagccccaat tgccccaatt 7981 gaccccaaat tgacccagta gcggacccaa ccccggcgag agcccccttc accccacata 8041 tcaaacctcc cccggttccc acacttgccg ttaagggcgt agggtactgc agtctggaat 8101 ctacgcttgt tcagactttg tactagtttc tttgtctggc catccgggta acccatgccg 8161 gacgcaaaat agactactga aaattttttt gctttgtggt tgggacttta gccaagggta 8221 taaaagacca ccgtccccga attacctttc ctcttctttt ctctctctcc ttgtcaactc 8281 acacccgaag gatccACAAc cacaATGGAT AAGAAATACT CCATTGGCCT GGACATCGGA 8341 ACCAACTCCG TGGGTTGGGC CGTGATCACC GATGAGTACA AGGTGCCCTC TAAGAAATTC 8401 AAGGTCCTGG GCAACACCGA CCGACACTCC ATCAAGAAGA ACCTGATCGG CGCTCTGCTC 8461 TTCGACTCTG GCGAGACTGC TGAGGCCACC CGACTGAAGC GAACCGCTCG AAGACGATAC 8521 ACCCGAAGAA AGAACCGAAT CTGTTACCTG CAGGAGATCT TCTCTAACGA GATGGCCAAG 8581 GTGGACGACT CTTTCTTCCA CCGACTGGAG GAGTCTTTCC TGGTGGAGGA GGACAAGAAG 8641 CACGAGCGAC ACCCCATCTT CGGCAACATC GTGGACGAGG TGGCCTACCA CGAGAAGTAC 8701 CCCACCATCT ACCACCTGCG AAAGAAGCTG GTGGACTCTA CCGACAAGGC CGACCTGCGA 8761 CTGATCTACC TGGCCCTGGC CCACATGATC AAGTTCCGAG GCCACTTCCT GATCGAGGGC 8821 GACCTGAACC CCGACAACTC TGACGTGGAC AAGCTGTTCA TCCAGCTGGT GCAGACCTAC 8881 AACCAGCTCT TCGAAGAGAA CCCCATTAAC GCTTCTGGCG TGGATGCTAA GGCCATCCTG 8941 TCTGCCCGAC TGTCTAAGTC TCGACGACTC GAGAACCTGA TTGCTCAGCT CCCCGGAGAG 9001 AAGAAGAACG GTCTGTTCGG AAACCTGATT GCTCTGTCCC TGGGTCTGAC CCCTAACTTC 9061 AAGTCCAACT TCGATCTGGC TGAGGACGCT AAGCTGCAGC TGTCTAAGGA CACCTACGAC 9121 GATGACCTGG ATAACCTGCT CGCCCAGATT GGCGACCAGT ACGCCGACCT GTTCCTGGCC 9181 GCCAAGAACC TGTCTGACGC CATCCTGCTG TCTGACATCC TGCGAGTGAA CACCGAGATC 9241 ACCAAGGCCC CCCTGTCTGC CTCCATGATT AAGCGATACG ATGAGCACCA CCAGGATCTG 9301 ACCCTCCTCA AGGCTCTGGT CCGACAGCAG CTGCCCGAGA AGTACAAGGA GATTTTCTTC 9361 GACCAGTCTA AGAACGGCTA CGCCGGCTAC ATCGACGGCG GCGCCTCTCA GGAGGAGTTC 9421 TACAAGTTCA TTAAGCCCAT CCTGGAGAAG ATGGACGGAA CCGAGGAACT GCTCGTGAAG 9481 CTGAACCGAG AGGACCTCCT GCGAAAGCAG CGAACCTTCG ACAACGGCTC TATCCCCCAC 9541 CAGATCCACC TGGGCGAGCT GCACGCCATC CTGCGACGAC AGGAGGACTT CTACCCCTTC 9601 CTGAAGGACA ACCGAGAGAA GATCGAGAAG ATCCTGACCT TCCGAATCCC CTACTACGTG 9661 GGaCCCCTGG CCCGAGGaAA CTCTCGATTC GCtTGGATGA CCCGAAAGTC TGAGGAGACT 9721 ATTACCCCCT GGAACTTCGA GGAGGTGGTG GATAAGGGCG CCTCTGCTCA GTCTTTCATC 9781 GAGCGAATGA CCAACTTCGA CAAGAACCTC CCCAACGAGA AGGTCCTGCC CAAGCACTCT 9841 CTGCTCTACG AGTACTTCAC CGTCTACAAC GAGCTCACCA AGGTCAAGTA CGTGACCGAG 9901 GGAATGCGAA AGCCCGCTTT CCTGTCTGGA GAGCAGAAGA AGGCTATTGT GGATCTGCTC 9961 TTCAAGACTA ACCGAAAGGT CACCGTCAAG CAGCTGAAGG AGGATTACTT CAAGAAGATT 10021 GAGTGTTTCG ATTCTGTCGA GATCTCCGGC GTCGAGGACC GATTCAACGC CTCTCTGGGT 10081 ACCTACCACG ACCTGCTGAA GATTATCAAG GACAAGGATT TCCTGGATAA CGAGGAGAAC 10141 GAGGATATTC TCGAGGACAT TGTCCTGACC CTCACCCTGT TCGAGGATCG AGAGATGATT 10201 GAGGAGCGAC TCAAGACCTA CGCTCACCTG TTCGACGACA AGGTGATGAA GCAGCTGAAG 10261 CGACGACGAT ACACCGGCTG GGGCCGACTG TCTCGAAAGC TGATCAACGG CATCCGAGAC 10321 AAGCAGTCTG GCAAGACCAT CCTGGACTTC CTGAAGTCTG ACGGCTTCGC CAACCGAAAC 10381 TTCATGCAGC TGATCCACGA CGACTCTCTG ACCTTCAAGG AGGACATCCA GAAGGCCCAG 10441 GTGTCTGGCC AGGGCGACTC TCTGCACGAG CACATCGCCA ACCTGGCCGG CTCTCCCGCC 10501 ATTAAGAAAG GTATCCTGCA GACCGTCAAG GTGGTCGATG AGCTCGTCAA GGTGATGGGC 10561 CGACACAAGC CCGAGAACAT TGTCATTGAG ATGGCTCGAG AGAACCAGAC TACTCAGAAG 10621 GGTCAGAAAA ACTCCCGAGA GCGAATGAAG CGAATTGAGG AAGGTATTAA GGAGCTGGGC 10681 TCCCAGATTC TCAAGGAGCA TCCCGTGGAG AACACTCAGC TCCAGAACGA GAAGCTGTAC 10741 CTGTACTATC TGCAGAACGG TCGAGACATG TACGTCGACC AGGAGCTGGA TATCAACCGA 10801 CTCTCCGACT ACGATGTGGA CCACATTGTG CCCCAGTCCT TCCTGAAGGA CGATTCTATC 10861 GATAACAAGG TGCTGACCCG ATCCGACAAG AACCGAGGCA AGTCTGACAA CGTGCCCTCC 10921 GAGGAGGTGG TCAAGAAGAT GAAGAACTAC TGGCGACAGC TGCTGAACGC CAAGCTGATT 10981 ACCCAGCGAA AGTTCGACAA CCTGACCAAG GCCGAGCGAG GCGGCCTGTC TGAGCTGGAC 11041 AAGGCCGGCT TCATCAAGCG ACAGCTGGTG GAGACTCGAC AGATCACCAA GCACGTGGCC 11101 CAGATCCTGG ACTCTCGAAT GAACACCAAG TACGACGAGA ACGACAAGCT GATCCGAGAG 11161 GTGAAGGTGA TCACCCTGAA GTCTAAGCTG GTGTCTGACT TCCGAAAGGA CTTCCAGTTC 11221 TACAAGGTGC GAGAGATTAA CAACTACCAC CACGCCCACG ATGCCTACCT GAACGCTGTC 11281 GTGGGCACCG CCCTCATCAA GAAGTATCCC AAGCTGGAGT CCGAGTTCGT CTACGGCGAC 11341 TACAAGGTCT ACGATGTGCG AAAAATGATT GCCAAGTCCG AGCAGGAGAT TGGCAAGGCT 11401 ACCGCCAAGT ACTTCTTCTA CTCCAACATT ATGAACTTCT TCAAGACCGA GATTACCCTG 11461 GCTAACGGCG AGATTCGAAA GCGACCCCTC ATTGAGACTA ACGGAGAGAC TGGTGAGATC 11521 GTGTGGGACA AGGGACGAGA CTTCGCCACC GTGCGAAAGG TGCTGTCTAT GCCCCAGGTG 11581 AACATCGTGA AGAAGACCGA GGTGCAGACC GGAGGTTTCT CTAAGGAGTC CATCCTGCCC 11641 AAGCGAAACT CTGACAAGCT GATCGCCCGA AAGAAGGACT GGGACCCCAA GAAGTACGGA 11701 GGTTTCGACT CTCCCACCGT GGCTTACTCT GTGCTGGTGG TGGCCAAGGT GGAGAAGGGC 11761 AAGTCTAAGA AGCTGAAGTC TGTGAAGGAG CTGCTGGGCA TTACCATCAT GGAGCGATCT 11821 TCTTTCGAGA AGAACCCCAT TGACTTCCTG GAGGCCAAGG GATACAAGGA GGTGAAGAAA 11881 GATCTGATTA TCAAGCTCCC CAAGTACTCT CTGTTCGAGC TGGAGAACGG ACGAAAGCGA 11941 ATGCTGGCCT CTGCCGGCGA GCTGCAGAAG GGAAACGAGC TGGCCCTGCC CTCCAAGTAC 12001 GTCAACTTCC TGTACCTCGC CTCCCATTAC GAGAAGCTGA AGGGCTCTCC CGAGGATAAC 12061 GAGCAGAAGC AGCTCTTCGT GGAGCAGCAT AAGCACTACC TGGACGAGAT CATCGAGCAG 12121 ATTTCTGAGT TCTCTAAGCG AGTGATCCTG GCTGACGCCA ACCTGGATAA GGTGCTGTCT 12181 GCTTACAACA AGCACCGAGA CAAGCCCATT CGAGAGCAGG CTGAGAACAT CATTCACCTG 12241 TTCACCCTGA CCAACCTGGG AGCCCCCGCT GCCTTCAAGT ACTTCGACAC CACCATCGAC 12301 CGAAAGCGAT ACACCTCTAC CAAGGAGGTG CTGGACGCCA CCCTGATCCA CCAGTCTATC 12361 ACCGGCCTGT ACGAGACTCG AATCGACCTG TCTCAGCTGG GCGGCGACTC TCGAGCCGAC 12421 CCCAAGAAGA AGCGAAAGGT GTAAGCTAGC CTCATGTAAT TAGTTATGTC ACGCTTACAT 12481 TCACGCCCTC CCCCCACATC CGCTCTAACC GAAAAGGAAG GAGTTAGACA ACCTGAAGTC 12541 TAGGTCCCTA TTTATTTTTT TATAGTTATG TTAGTATTAA GAACGTTATT TATATTTCAA 12601 ATTTTTCTTT TTTTTCTGTA CAGACGCGTG TACGCATGTA ACATTATACT GAAAACCTTG 12661 CTTGAGAAGG TTTTGGGACG CTCGAAGGCT TTAATTTGCA AGCTTGGCGG TCT //