LOCUS       GGA_CrCas9_p8UASTEF-EY 12713 bp ds-DNA     circular     22-SEP-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3696..3773
                     /label="sgRNA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    7..10
                     /label="Geneious type: ligation"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(1025..1885)
                     /label="ampR (rev) CDS"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6994..8295
                     /label="p8UASxpr-Tef"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2125..2128
                     /label="Geneious type: ligation"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3507..3531
                     /label="misc_feature"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     stem_loop       complement(2097..2116)
                     /label="stem_loop"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    6..10
                     /label="PstI misc_feature"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2600..2799
                     /label="Geneious type: promoter prokaryote"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2130..2134
                     /label="Bbsi misc_feature"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2151..2568
                     /label="Promotor_SCR1-tRNA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    6151..6278
                     /label="tLIP2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    11..11
                     /label="misc_feature"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    12459..12704
                     /label="CYC-t"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    8305..12444
                     /label="Codon opt CAS 9 SV40"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2497..2568
                     /label="tRNA-Gly"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    12..83
                     /label="E. coli his operon terminator misc_feature"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(147..166)
                     /label="primer_bind"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2151..2569
                     /label="sgRNA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2826..3500
                     /label="mrfp1 CDS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(1147..1178)
                     /label="primer_bind"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(251..865)
                     /label="rep(pMB1) (rev) CDS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     1147..1178
                     /label="primer_bind"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     2008..2027
                     /label="primer_bind"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6286..6711
                     /label="CloningVector"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     stem_loop       20..54
                     /label="stem_loop"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    12421..12441
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3862..3975
                     /label="Yl-ARS1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2136..2136
                     /label="Geneious type: Editing History Insertion"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2594..2597
                     /label="Geneious type: ligation"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4389..6146
                     /label="EYK"
                     /ApEinfo_revcolor=#3366ff
                     /ApEinfo_fwdcolor=#3366ff
     misc_feature    6712..6938
                     /label="ARS-Cen1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2084..2087)
                     /label="end of terminator misc_feature"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2125..2129
                     /label="EcoRI misc_feature"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    7020..7128
                     /label="1UAS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2808..2819
                     /label="Geneious type: RBS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2594..2599
                     /label="Geneious type: motif"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2134..2135
                     /label="Geneious type: Editing History Deletion"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1164..1164
                     /label="Geneious type: Editing History Replacement"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2594..3668
                     /label="RFP"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3540..3668
                     /label="Geneious type: Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4007..4379
                     /label="pTEF"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    266..266
                     /label="ORI misc_feature"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(5148..5169)
                     /label="ForT7Endon Test Rv_ EYK"
                     /note="sequence: CCTTGAACTTTACGAAGGATCG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          2143..2160
                     /label="Promoter PolIII_GGP_A_Fw"
                     /note="sequence: gggGGTCTCtGCCTcctgacgtCCCCAGTTGC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(8633..8654)
                     /label="Cas9_1_Rv"
                     /note="sequence: GGGTGTCGCTCGTGCTTCTTGT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          11121..11145
                     /label="Cas9_6_Fw"
                     /note="sequence: GAACACCAAGTACGACGAGAACGAC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3966..3988)
                     /label="DRSfinal_ARS_GGP_B_Rv"
                     /note="sequence: GGGGGTCTCtACCTAGCGGCCGCATACTACTG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(3648..3685)
                     /label="RFP_SfiI_Rv"
                     /note="sequence: ccGGCCCAGATGGCCtatataaacgcagaaaggcccac"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          2579..2614
                     /label="RFP_SfiI_Fw"
                     /note="sequence: ccGGCCATCTGGGCCctagagcaatacgcaaaccgc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          8305..8331
                     /label="Cas9_1_Fw"
                     /note="sequence: ATGGATAAGAAATACTCCATTGGCCTG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(2548..2579)
                     /label="promoter PolIII_BsmbI Sfi Avr_Rv"
                     /note="sequence: gggCCTAGGGGCCCAGATGGCCCGTCTCtCGAACCGGGGGCCCATCGATG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2548..2575)
                     /label="Promoter PolIII_BsmbI_Rv"
                     /note="sequence: CGTCTCtCGAACCGGGGGCCCATCGATG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(3749..3767)
                     /label="Verif sgRNA_Rv"
                     /note="sequence: AAGCACCGACTCGGTGCCA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2137..2156
                     /label="Promoter PolIII_GGP_O_Fw"
                     /note="sequence: GGGGGTCTCtCTGACCTgacgtCCCCAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          6913..6938
                     /label="ARS-Cen1_Verif_Fw"
                     /note="sequence: GATGGCATCCCTAAATTTGATGAAAG"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(3966..3987)
                     /label="DRSfinal_ARS_GGP_M_Rv"
                     /note="sequence: GGGGGTCTCtCGCAACCTAGCGGCCGCATACTACTG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2494..2518
                     /label="Verif sgRNA_Fw"
                     /note="sequence: CTTtGAAAAATACCTCTAATGCGCC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          11681..11702
                     /label="Cas9_7_Fw"
                     /note="sequence: GGGACCCCAAGAAGTACGGAGG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          4402..4423
                     /label="ForT7Endon test Fw_EYK"
                     /note="sequence: ATCTGTTCAACGAGACTGACGA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 tcttcctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
       61 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
      121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      241 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      661 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      901 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      961 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1141 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata
     1201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1321 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1381 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1441 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1501 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1561 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1621 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1681 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1741 tccagttcga tataacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1801 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1861 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1921 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1981 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2041 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2101 gctttcgcta aggatgattt ctggaattcg aagagcctCT GACCTgacgt CCCCAGTTGC
     2161 AAAAGTTGAC ACAACTCTAG ATCTGCTTCC AAATATAGAA TCATAACAAG GGTTAGGGTG
     2221 TGATTATATA ATATTGGTCT TAATTGATGT GCTAGGGCTT TAAAAGTTGG TTAAAATAAC
     2281 GCTCTAATGC CTTTTTAATA TATTGTCTTT TTCAAAATCT CAAATCGGAC ACTTCTTCGT
     2341 GTATGAGACT CCATTTTTTG GCTCCGTCAC GTGATATGTA TTATCAGCTA TAGTGGTGTA
     2401 AACAAAGTTT TTTACTAGCT GTAATGGCAT TTTGTCGGAG TGGTAAATCG CCTTCTTGTT
     2461 GTGCGTTCGA GTTCTGGACT CTGCACTGGG CTACTTtGAA AAATACCTCT AATGCGCCGA
     2521 TGGTTTAGTG GTAAAATCCA TCGTTGCCAT CGATGGGCCC CCGGTTCGaG AGACGgggcc
     2581 GGCCATCTGG GCCctagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2641 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2701 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     2761 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat
     2821 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
     2881 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
     2941 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
     3001 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
     3061 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     3121 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     3181 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     3241 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     3301 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     3361 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     3421 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     3481 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc
     3541 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     3601 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     3661 cgtttatata GGCCATCTGG GCCgggggCG TCTCtGAGCT AGAAATAGCA AGTTAAAATA
     3721 AGGCTAGTCC GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TTTacgtcTA
     3781 AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCAGATC
     3841 CTCTAGAGTC GAAGCGGCCG CTATGTCTGA TAAAAGGATG TAACATAGGC AAGCTGCTCG
     3901 TGAGTGTTGA GTACGAACCT TAGATCCAAA TCACCCGCAC CCACGGATAT ACTTGCTTGA
     3961 ATATACAGTA GTATGCGGCC GCTAGGTCTG TTATCCCTAG ATCGATATAG AGacTgggtt
     4021 ggcggcgcat ttgtgtccca aaaaacagcc ccaattgccc caattgaccc caaattgacc
     4081 cagtagcgga cccaaccccg gcgagagccc ccttcacccc acatatcaaa cctcccccgg
     4141 ttcccacact tgccgttaag ggcgtagggt actgcagtct ggaatctacg cttgttcaga
     4201 ctttgtacta gtttctttgt ctggccatcc gggtaaccca tgccggacgc aaaatagact
     4261 actgaaaatt tttttgcttt gtggttggga ctttagccaa gggtataaaa gaccaccgtc
     4321 cccgaattac ctttcctctt cttttctctc tctccttgtc aactcacacc cgaaggatcg
     4381 GATCCACAAT GTCCACAAAA CATCTGTTCA ACGAGACTGA CGAGCTCGTG CTCAAGTCTC
     4441 TCGAGGGAGT TCAGGCTTCC CGATCAGCGT CCATTCTCTC CCACAGATTC AAGGTGCTCT
     4501 ACAACGGAAC CCATTCCGCT GACCGAGTTG CAGTTCTTTC TGGAGGAGGC TCTGGCCACG
     4561 AGCCTGCCCA CGCCGGCTTT GTGGGAGACA ACATGCTGAC CGGTGCCATC TGTGGCCCCG
     4621 TCTTCGCGTC CCCTTCAGCC AAGCAGGTCG AGGCCGGCTG CAAGCTCGTG CCCTCCGACA
     4681 AGGGTCATAT TCTGGTCGTC ACCAACTACA CCGGCGACAT GTTGCATTTT GGCCTCGCGG
     4741 CCGAGAAGCT CAAGTCTCAG GGCCACAAGG TCGGCATCAT TAAGTCTGCC GACGATGTGG
     4801 CTGTCGACCG AAAGAGCGGT GGTCTGGTCG GTCGACGAGG ACTGGCTGGA ACTGTGCTTC
     4861 TGGACAAGAT TGTCGGAGGA GCTGCCTGGG ACAAGCTGTC CTTTGATGAA TGCATGGCCA
     4921 TTGGAACCGA GGTGGCCGAG AACACCGCAA CCGCCTCAAT TGGACTGGAC TACTGCCACG
     4981 TTCCTGGTCG ATCCGTTGAG AACCACGTGT CGCTCGACCA GAACGAGTGT CAATTCGGCC
     5041 TGGGTATTCA CAATGAGCCC GGAGTCAAGA CCATCAACCC CGTGCCTGCC CCTGAGTCCA
     5101 TGGTTGATAC TCTGCTCAAG TACCTAGTTT CGCAGGACGA CCCCGAGCGA TCCTTCGTAA
     5161 AGTTCAAGGA GGGCGACGAG GTCATTCTCC TGGCCAACAA CCTCGGTGGA ATTTCCACCA
     5221 TTGAGATGCG GGCTGCTGTC CAGCTTGCCC GAGAGCAACT CGAGAAGACC CACAAGATCA
     5281 AGTCTGTGCG AGTTCTGTGC GGCACCTTCA TGAGCTCTCT CAACGCTCCC GGCTTCTCTA
     5341 TCACCCTGGT GAACCTGTCT AACGGCTCCC ACAGCAAGAA CGTGCTCAAG TACCTGGACG
     5401 CTGTGTCCGA CGCCCCTGCC TGGGTCAATG TGGCCCCTCC TACCTCGGTC AAGCCCTTTA
     5461 TCAACGAGGA CAAGATCTTC GACGACGAGA CTTCCAACAT TAAGGCTCCC ACACTGGACA
     5521 TTCCTGAACA GACCGTGGTT GCTGCTCTCA CTCAGGCCTC CCAGAACATT ATCAAGGCCG
     5581 AGCCTCAGCT TACTGCATGG GATACCGAAA TGGGAGATGG AGACTGTGGA CACACCATTG
     5641 AACATGGCTG CCGAGCTCTT CTCGAGTACC TCAACAAGAA CAAGAGCGAC CCAAAGGCTC
     5701 TCGAGATCAT CCCCATTGTG CGGGCTGTCG TTCACATCAC CGAGGAGGAT ATGGGAGGCA
     5761 CCCTGGGAGC TATCTTCGGC ATTTTTTTTG CCTCTTTCCT TAACGCTCTT CTGCTGGACC
     5821 CTCTGTCCCA TAAGACTGAT GTCAATGTGA CTGATAAGCT CGTCAACGCT GCTAACACCG
     5881 GTCTGGAAAG TCTCATGAAC CACACTCCTG CTCGACCTGG TGACCGAACT GTCATGGACG
     5941 TGTTGATCCC CTACGTGCAG TCTCTGGTGT CCACCAAGGA TATCAAGGAG GCCGCTCTCA
     6001 AGGCCAAGCA GGCTGCCGAG GGCACCAAGA AGATCAAGCC TCGTCTTGGA CGAGCAGTCT
     6061 ATGTTGGGGA GAAGGACGGT GAGCTTCCTC CCGACCCTGG AGCTTGGGCT GTCTACGAGC
     6121 TGGTTGATGG TTTTGCCAAC CACAAGTAGC ctagggtgtc tgtggtatct aagctattta
     6181 tcactcttta caacttctac ctcaactatc tactttaata aatgaatatc gtttattctc
     6241 tatgattact gtatatgcgt tcctctaaga caaatcgaat GACGGGGCCC TTTCGTCaCG
     6301 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGtG ACGGTCACAG
     6361 CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG
     6421 GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC
     6481 ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT
     6541 CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC
     6601 GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT
     6661 CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTCGA GCTCGGTACC CGCTTTTCGT
     6721 AGATAATGGA ATACAAATGG ATATCCAGAG TATACACATG GATAGTATAC ACTGACACGA
     6781 CAATTCTGTA TCTCTTTATG TTAACTACTG TGAGGCGTTA AATAGAGCTT GATATATAAA
     6841 ATGTTACATT TCACAGTCTG AACTTTTGCA GATTACCTAA TTTGGTAAGA TATTAATTAT
     6901 GAACTGAAAG TTGATGGCAT CCCTAAATTT GATGAAAGGG GGAaCCCCCG GGTTCGAAGG
     6961 TACCAAGGAA GCATGCGGTA CCCGAATTCG CTTCGATACG CGTatcgatg atacgcgtcc
     7021 atgCtgaggt gtctcacaag tgccgtgcag tcccgccccc acttgcttct ctttgtgtgt
     7081 agtgtacgta cattatcgag agggttgttc ccgcccacct cgatccggca tgctgaggtg
     7141 tctcacaagt gccgtgcagt cccgccccca cttgcttctc tttgtgtgta gtgtacgtac
     7201 attatcgaga gggttgttcc cgcccacctc gatccggcat gctgaggtgt ctcacaagtg
     7261 ccgtgcagtc ccgcccccac ttgcttctct ttgtgtgtag tgtacgtaca ttatcgagag
     7321 ggttgttccc gcccacctcg atccggcatg ctgaggtgtc tcacaagtgc cgtgcagtcc
     7381 cgcccccact tgcttctctt tgtgtgtagt gtacgtacat tatcgagagg gttgttcccg
     7441 cccacctcga tccgGcatgC tgaggtgtct cacaagtgcc gtgcagtccc gcccccactt
     7501 gcttctcttt gtgtgtagtg tacgtacatt atcgagaggg ttgttcccgc ccacctcgat
     7561 ccggcatgct gaggtgtctc acaagtgccg tgcagtcccg cccccacttg cttctctttg
     7621 tgtgtagtgt acgtacatta tcgagagggt tgttcccgcc cacctcgatc cggcatgctg
     7681 aggtgtctca caagtgccgt gcagtcccgc ccccacttgc ttctctttgt gtgtagtgta
     7741 cgtacattat cgagagggtt gttcccgccc acctcgatcc ggcatgctga ggtgtctcac
     7801 aagtgccgtg cagtcccgcc cccacttgct tctctttgtg tgtagtgtac gtacattatc
     7861 gagagggttg ttcccgccca cctcgatccg gcatgcactg atcacgggca aaagtgcttc
     7921 gatagagagg gggttggcgg cgcatttgtg tcccaaaaaa cagccccaat tgccccaatt
     7981 gaccccaaat tgacccagta gcggacccaa ccccggcgag agcccccttc accccacata
     8041 tcaaacctcc cccggttccc acacttgccg ttaagggcgt agggtactgc agtctggaat
     8101 ctacgcttgt tcagactttg tactagtttc tttgtctggc catccgggta acccatgccg
     8161 gacgcaaaat agactactga aaattttttt gctttgtggt tgggacttta gccaagggta
     8221 taaaagacca ccgtccccga attacctttc ctcttctttt ctctctctcc ttgtcaactc
     8281 acacccgaag gatccACAAc cacaATGGAT AAGAAATACT CCATTGGCCT GGACATCGGA
     8341 ACCAACTCCG TGGGTTGGGC CGTGATCACC GATGAGTACA AGGTGCCCTC TAAGAAATTC
     8401 AAGGTCCTGG GCAACACCGA CCGACACTCC ATCAAGAAGA ACCTGATCGG CGCTCTGCTC
     8461 TTCGACTCTG GCGAGACTGC TGAGGCCACC CGACTGAAGC GAACCGCTCG AAGACGATAC
     8521 ACCCGAAGAA AGAACCGAAT CTGTTACCTG CAGGAGATCT TCTCTAACGA GATGGCCAAG
     8581 GTGGACGACT CTTTCTTCCA CCGACTGGAG GAGTCTTTCC TGGTGGAGGA GGACAAGAAG
     8641 CACGAGCGAC ACCCCATCTT CGGCAACATC GTGGACGAGG TGGCCTACCA CGAGAAGTAC
     8701 CCCACCATCT ACCACCTGCG AAAGAAGCTG GTGGACTCTA CCGACAAGGC CGACCTGCGA
     8761 CTGATCTACC TGGCCCTGGC CCACATGATC AAGTTCCGAG GCCACTTCCT GATCGAGGGC
     8821 GACCTGAACC CCGACAACTC TGACGTGGAC AAGCTGTTCA TCCAGCTGGT GCAGACCTAC
     8881 AACCAGCTCT TCGAAGAGAA CCCCATTAAC GCTTCTGGCG TGGATGCTAA GGCCATCCTG
     8941 TCTGCCCGAC TGTCTAAGTC TCGACGACTC GAGAACCTGA TTGCTCAGCT CCCCGGAGAG
     9001 AAGAAGAACG GTCTGTTCGG AAACCTGATT GCTCTGTCCC TGGGTCTGAC CCCTAACTTC
     9061 AAGTCCAACT TCGATCTGGC TGAGGACGCT AAGCTGCAGC TGTCTAAGGA CACCTACGAC
     9121 GATGACCTGG ATAACCTGCT CGCCCAGATT GGCGACCAGT ACGCCGACCT GTTCCTGGCC
     9181 GCCAAGAACC TGTCTGACGC CATCCTGCTG TCTGACATCC TGCGAGTGAA CACCGAGATC
     9241 ACCAAGGCCC CCCTGTCTGC CTCCATGATT AAGCGATACG ATGAGCACCA CCAGGATCTG
     9301 ACCCTCCTCA AGGCTCTGGT CCGACAGCAG CTGCCCGAGA AGTACAAGGA GATTTTCTTC
     9361 GACCAGTCTA AGAACGGCTA CGCCGGCTAC ATCGACGGCG GCGCCTCTCA GGAGGAGTTC
     9421 TACAAGTTCA TTAAGCCCAT CCTGGAGAAG ATGGACGGAA CCGAGGAACT GCTCGTGAAG
     9481 CTGAACCGAG AGGACCTCCT GCGAAAGCAG CGAACCTTCG ACAACGGCTC TATCCCCCAC
     9541 CAGATCCACC TGGGCGAGCT GCACGCCATC CTGCGACGAC AGGAGGACTT CTACCCCTTC
     9601 CTGAAGGACA ACCGAGAGAA GATCGAGAAG ATCCTGACCT TCCGAATCCC CTACTACGTG
     9661 GGaCCCCTGG CCCGAGGaAA CTCTCGATTC GCtTGGATGA CCCGAAAGTC TGAGGAGACT
     9721 ATTACCCCCT GGAACTTCGA GGAGGTGGTG GATAAGGGCG CCTCTGCTCA GTCTTTCATC
     9781 GAGCGAATGA CCAACTTCGA CAAGAACCTC CCCAACGAGA AGGTCCTGCC CAAGCACTCT
     9841 CTGCTCTACG AGTACTTCAC CGTCTACAAC GAGCTCACCA AGGTCAAGTA CGTGACCGAG
     9901 GGAATGCGAA AGCCCGCTTT CCTGTCTGGA GAGCAGAAGA AGGCTATTGT GGATCTGCTC
     9961 TTCAAGACTA ACCGAAAGGT CACCGTCAAG CAGCTGAAGG AGGATTACTT CAAGAAGATT
    10021 GAGTGTTTCG ATTCTGTCGA GATCTCCGGC GTCGAGGACC GATTCAACGC CTCTCTGGGT
    10081 ACCTACCACG ACCTGCTGAA GATTATCAAG GACAAGGATT TCCTGGATAA CGAGGAGAAC
    10141 GAGGATATTC TCGAGGACAT TGTCCTGACC CTCACCCTGT TCGAGGATCG AGAGATGATT
    10201 GAGGAGCGAC TCAAGACCTA CGCTCACCTG TTCGACGACA AGGTGATGAA GCAGCTGAAG
    10261 CGACGACGAT ACACCGGCTG GGGCCGACTG TCTCGAAAGC TGATCAACGG CATCCGAGAC
    10321 AAGCAGTCTG GCAAGACCAT CCTGGACTTC CTGAAGTCTG ACGGCTTCGC CAACCGAAAC
    10381 TTCATGCAGC TGATCCACGA CGACTCTCTG ACCTTCAAGG AGGACATCCA GAAGGCCCAG
    10441 GTGTCTGGCC AGGGCGACTC TCTGCACGAG CACATCGCCA ACCTGGCCGG CTCTCCCGCC
    10501 ATTAAGAAAG GTATCCTGCA GACCGTCAAG GTGGTCGATG AGCTCGTCAA GGTGATGGGC
    10561 CGACACAAGC CCGAGAACAT TGTCATTGAG ATGGCTCGAG AGAACCAGAC TACTCAGAAG
    10621 GGTCAGAAAA ACTCCCGAGA GCGAATGAAG CGAATTGAGG AAGGTATTAA GGAGCTGGGC
    10681 TCCCAGATTC TCAAGGAGCA TCCCGTGGAG AACACTCAGC TCCAGAACGA GAAGCTGTAC
    10741 CTGTACTATC TGCAGAACGG TCGAGACATG TACGTCGACC AGGAGCTGGA TATCAACCGA
    10801 CTCTCCGACT ACGATGTGGA CCACATTGTG CCCCAGTCCT TCCTGAAGGA CGATTCTATC
    10861 GATAACAAGG TGCTGACCCG ATCCGACAAG AACCGAGGCA AGTCTGACAA CGTGCCCTCC
    10921 GAGGAGGTGG TCAAGAAGAT GAAGAACTAC TGGCGACAGC TGCTGAACGC CAAGCTGATT
    10981 ACCCAGCGAA AGTTCGACAA CCTGACCAAG GCCGAGCGAG GCGGCCTGTC TGAGCTGGAC
    11041 AAGGCCGGCT TCATCAAGCG ACAGCTGGTG GAGACTCGAC AGATCACCAA GCACGTGGCC
    11101 CAGATCCTGG ACTCTCGAAT GAACACCAAG TACGACGAGA ACGACAAGCT GATCCGAGAG
    11161 GTGAAGGTGA TCACCCTGAA GTCTAAGCTG GTGTCTGACT TCCGAAAGGA CTTCCAGTTC
    11221 TACAAGGTGC GAGAGATTAA CAACTACCAC CACGCCCACG ATGCCTACCT GAACGCTGTC
    11281 GTGGGCACCG CCCTCATCAA GAAGTATCCC AAGCTGGAGT CCGAGTTCGT CTACGGCGAC
    11341 TACAAGGTCT ACGATGTGCG AAAAATGATT GCCAAGTCCG AGCAGGAGAT TGGCAAGGCT
    11401 ACCGCCAAGT ACTTCTTCTA CTCCAACATT ATGAACTTCT TCAAGACCGA GATTACCCTG
    11461 GCTAACGGCG AGATTCGAAA GCGACCCCTC ATTGAGACTA ACGGAGAGAC TGGTGAGATC
    11521 GTGTGGGACA AGGGACGAGA CTTCGCCACC GTGCGAAAGG TGCTGTCTAT GCCCCAGGTG
    11581 AACATCGTGA AGAAGACCGA GGTGCAGACC GGAGGTTTCT CTAAGGAGTC CATCCTGCCC
    11641 AAGCGAAACT CTGACAAGCT GATCGCCCGA AAGAAGGACT GGGACCCCAA GAAGTACGGA
    11701 GGTTTCGACT CTCCCACCGT GGCTTACTCT GTGCTGGTGG TGGCCAAGGT GGAGAAGGGC
    11761 AAGTCTAAGA AGCTGAAGTC TGTGAAGGAG CTGCTGGGCA TTACCATCAT GGAGCGATCT
    11821 TCTTTCGAGA AGAACCCCAT TGACTTCCTG GAGGCCAAGG GATACAAGGA GGTGAAGAAA
    11881 GATCTGATTA TCAAGCTCCC CAAGTACTCT CTGTTCGAGC TGGAGAACGG ACGAAAGCGA
    11941 ATGCTGGCCT CTGCCGGCGA GCTGCAGAAG GGAAACGAGC TGGCCCTGCC CTCCAAGTAC
    12001 GTCAACTTCC TGTACCTCGC CTCCCATTAC GAGAAGCTGA AGGGCTCTCC CGAGGATAAC
    12061 GAGCAGAAGC AGCTCTTCGT GGAGCAGCAT AAGCACTACC TGGACGAGAT CATCGAGCAG
    12121 ATTTCTGAGT TCTCTAAGCG AGTGATCCTG GCTGACGCCA ACCTGGATAA GGTGCTGTCT
    12181 GCTTACAACA AGCACCGAGA CAAGCCCATT CGAGAGCAGG CTGAGAACAT CATTCACCTG
    12241 TTCACCCTGA CCAACCTGGG AGCCCCCGCT GCCTTCAAGT ACTTCGACAC CACCATCGAC
    12301 CGAAAGCGAT ACACCTCTAC CAAGGAGGTG CTGGACGCCA CCCTGATCCA CCAGTCTATC
    12361 ACCGGCCTGT ACGAGACTCG AATCGACCTG TCTCAGCTGG GCGGCGACTC TCGAGCCGAC
    12421 CCCAAGAAGA AGCGAAAGGT GTAAGCTAGC CTCATGTAAT TAGTTATGTC ACGCTTACAT
    12481 TCACGCCCTC CCCCCACATC CGCTCTAACC GAAAAGGAAG GAGTTAGACA ACCTGAAGTC
    12541 TAGGTCCCTA TTTATTTTTT TATAGTTATG TTAGTATTAA GAACGTTATT TATATTTCAA
    12601 ATTTTTCTTT TTTTTCTGTA CAGACGCGTG TACGCATGTA ACATTATACT GAAAACCTTG
    12661 CTTGAGAAGG TTTTGGGACG CTCGAAGGCT TTAATTTGCA AGCTTGGCGG TCT
//