LOCUS       Exported                5076 bp ds-DNA     circular SYN 16-JUL-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pYPQ141-ZmUbi-RZ-Bth
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 16, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..2178
                     /label=source
                     /note="ApEinfo_fwdcolor=pink 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink 
                     dnas_title=pYPQ141-ZmUbi-RZ-Lb"
     source          1..162
                     /label=source(1)
                     /note="source(1)"
                     /note="ApEinfo_fwdcolor=pink 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_label=source ApEinfo_revcolor=pink
                     dnas_title=pYPQ104_correct_"
     source          2131..2135
                     /label=source(2)
                     /note="source(2)"
                     /note="ApEinfo_fwdcolor=pink 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_label=source ApEinfo_revcolor=pink
                     dnas_title=RZ-AsCpf1-OsYsA-gR1"
     source          2283..5076
                     /label=source(4)
                     /note="source(4)"
                     /note="ApEinfo_fwdcolor=pink 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_label=source ApEinfo_revcolor=pink
                     dnas_title=pYPQ141-ZmUbi-RZ-Lb"
     source          2383..2395
                     /label=source(3)
                     /note="source(3)"
                     /note="ApEinfo_fwdcolor=pink 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_label=source ApEinfo_revcolor=pink
                     dnas_title=DA1-RZ-gR1"
     misc_feature    2..25
                     /label=M13-F1
                     /label=PCR_primer
                     /note="M13-F1"
                     /note="M13-F1 ApEinfo_fwdcolor=pink 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
     misc_feature    37..149
                     /label=attL5
                     /note="attL5"
                     /note="ApEinfo_fwdcolor=#c0c0c0 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=#c0c0c0 dnas_title=attL5 
                     vntifkey=21"
     promoter        167..2129
                     /label=Ubi1 (maize ubiquitin promoter 1)
                     /note="Ubi1 (maize ubiquitin promoter 1) promoter"
                     /note="Ubi1 (maize ubiquitin promoter 1) 
                     ApEinfo_fwdcolor=#346ee0 ApEinfo_graphicformat=arrow_data 
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0 
                     ApEinfo_revcolor=#346ee0"
     misc_feature    2136..2141
                     /label=1st 6nt complementary
                     /note="1st 6nt complementary"
     stem_loop       2142..2178
                     /label=Hammerhead ribozyme
                     /note="Hammerhead ribozyme"
                     /note="Hammerhead ribozyme ApEinfo_fwdcolor=pink 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
     misc_feature    2179..2282
                     /label=BthC2c1 sgRNA backbone
                     /note="BthC2c1 sgRNA backbone"
     misc_recomb     2283..2314
                     /label=Guide RNA cloning site
                     /note="Guide RNA cloning site"
                     /note="Guide RNA cloning site ApEinfo_fwdcolor=#8080ff 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=#da6a3b"
     stem_loop       2315..2382
                     /label=HDV ribozyme
                     /note="HDV ribozyme"
                     /note="HDV ribozyme ApEinfo_fwdcolor=pink 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=pink"
     misc_feature    2402..2501
                     /label=attL2
                     /note="attL2"
                     /note="ApEinfo_fwdcolor=#ff0080 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=#ff0080 dnas_title=attL2 
                     vntifkey=21"
     misc_feature    2531..2565
                     /label=extra seq
                     /note="extra seq"
                     /note="extra seq ApEinfo_fwdcolor=#7eff74 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=#7eff74"
     primer_bind     complement(2573..2593)
                     /label=M13-rev
                     /note="M13-rev"
                     /note="ApEinfo_fwdcolor=cyan 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=green dnas_title=M13-rev 
                     vntifkey=28"
     misc_feature    2671..2804
                     /label=Spn promoter
                     /note="Spn promoter"
                     /note="ApEinfo_fwdcolor=#2fec13 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=#2fec13 dnas_title=Spn 
                     promoter vntifkey=21"
     misc_feature    2705
                     /label=G to A mutation
                     /note="G to A mutation"
     misc_feature    2805..3815
                     /label=SpnR
                     /note="SpnR"
                     /note="ApEinfo_fwdcolor=#f70938 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=#f70938 dnas_title=SpnR 
                     vntifkey=21"
     misc_feature    3882..4555
                     /label=pUC origin
                     /note="pUC origin"
                     /note="ApEinfo_fwdcolor=cyan 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=green dnas_title=pUC 
                     origin vntifkey=21"
ORIGIN
        1 tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc aaataatgat
       61 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
      121 aatgccaact ttgtatacaa aagttgcccc atggcgttcc ctctagagag ataatgagca
      181 ttgcatgtct aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg
      241 cagtttatct atctttatac atatatttaa actttactct acgaataata taatctatag
      301 tactacaata atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa
      361 aggacaattg agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg
      421 ttctcctttt tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac
      481 atccatttag ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt
      541 tattctattt tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa
      601 taatttagat ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa
      661 gaaattaaaa aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta
      721 aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca
      781 agcgaagcag acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc
      841 tccaccgttg gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg
      901 tgagccggca cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt
      961 cctttcccac cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct
     1021 ccacaccctc tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc
     1081 ccccaaatcc acccgtcggc acctccgctt caaggtacgc cgctcgtcct cccccccccc
     1141 cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac
     1201 ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta
     1261 cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt
     1321 ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt
     1381 gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt
     1441 gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt
     1501 gggcggtcgt tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat
     1561 taattttgga tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg
     1621 atggaaatat cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac
     1681 agagatgctt tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc
     1741 gttctagatc ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac
     1801 tgtatgtgtg tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc
     1861 taggataggt atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca
     1921 gcatctattc atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt
     1981 ataattattt tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt
     2041 tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct
     2101 caccctgttg tttggtgtta cttctgcagg gatccctcgc cctgatgagt ccgtgaggac
     2161 gaaacgagta agctcgtcgg cgaggttctg tcttttggtc aggacaaccg tctagctata
     2221 agtgctgcag gggtgtgaga aactcctatt gctggacgat gtctctttcg aggcattagc
     2281 acgagacgga gctcagtctg accgcggcgt ctctggccgg catggtccca gcctcctcgc
     2341 tggcgccggc tgggcaacat gcttcggcat ggcgaatggg acggtacccg gccggaattc
     2401 gacccagctt tcttgtacaa agttggcatt ataaaaaata attgctcatc aatttgttgc
     2461 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     2521 tatagtgagt cgtattacat ggtcatagca tcccctatag tgagtcgtat tacatggtca
     2581 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     2641 ataaaaatat atcatcatgc ctcctctgga ccagccagga cagaaatgcc tcgacttcgc
     2701 tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     2761 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     2821 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     2881 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     2941 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     3001 gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3061 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3121 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3181 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3241 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3301 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3361 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3421 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3481 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3541 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     3601 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     3661 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     3721 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     3781 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     3841 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3901 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3961 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4021 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4081 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4141 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4201 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4261 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     4321 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4381 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4441 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4501 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4561 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4621 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4681 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4741 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgctc
     4801 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4861 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4921 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4981 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     5041 agttccctac tctcgcgtta acgcttgcat ggatgt
//
