LOCUS       Exported                6106 bp ds-DNA     circular SYN 09-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ciaran Kelly
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 9, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        7..116
                     /label=kan_promoter
     CDS             117..932
                     /label=kan
     misc_feature    933..966
                     /label=Native rhaS RBS 72 TIR
                     /note="TIR:72"
     CDS             967..1803
                     /codon_start=1
                     /transl_table=11
                     /gene="rhaS"
                     /gene_synonym="ECK3898; JW3876; rhaC2"
                     /locus_tag="b3905"
                     /product="transcriptional activator of rhaBAD and rhaT"
                     /function="regulator; Degradation of small molecules: 
                     Carbon compounds"
                     /label=rhaS
                     /note="positive regulator for rhaBAD operon"
                     /db_xref="GI:16131745"
                     /db_xref="ASAP:ABE-0012745"
                     /db_xref="UniProtKB/Swiss-Prot:P09377"
                     /db_xref="EcoGene:EG10843"
                     /db_xref="GeneID:948397"
                     /protein_id="NP_418341.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     gene            967..1803
                     /gene="rhaS"
                     /gene_synonym="ECK3898; JW3876; rhaC2"
                     /locus_tag="b3905"
                     /label=rhaS
                     /db_xref="EcoGene:EG10843"
                     /db_xref="GeneID:948397"
     misc_feature    1687..1689
                     /product="binds to sigma70 residue R599"
                     /label=important for sigma 70 binding
     misc_feature    1714..1716
                     /product="binds to sigma70 K593"
                     /label=important for sigma 70 binding
     misc_recomb     1804..2842
                     /label=Homology Right to ndhB
     rep_origin      3158..3987
                     /label=p15A
     misc_recomb     4163..5177
                     /label=Homology Left to ndhB
     terminator      5184..5230
                     /label=ECK120015170
     promoter        5231..5352
                     /label=Rhamose Promoter
     misc_binding    5247..5262
                     /label=CRP binding site
                     /bound_moiety="CRP"
     misc_binding    5266..5282
                     /label=operator 1
                     /bound_moiety="RhaS"
     misc_binding    5299..5314
                     /label=operator 2
                     /bound_moiety="RhaS"
     -35_signal      5312..5317
                     /label=-35 box for PrhaBAD
     -10_signal      5335..5340
                     /label=-10 box for PrhaBAD
     misc_feature    5347
                     /label=TSS +1
     RBS             5353..5386
                     /label=RBS_synth_Psll1120-YFP
     CDS             5387..6106
                     /label=YFP (BBa_E0030)
ORIGIN
        1 ggatccaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
       61 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
      121 gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac atggatgctg
      181 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
      241 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
      301 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
      361 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
      421 ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
      481 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
      541 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
      601 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
      661 tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
      721 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
      781 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
      841 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
      901 agtttcattt gatgctcgat gagtttttct aatatttcgc cgtgttgacg acatcaggag
      961 gccagtatga ccgtattaca tagtgtggat ttttttccgt ctggtaacgc gtccgtggcg
     1021 atagaacccc ggctcccgca ggcggatttt cctgaacatc atcatgattt tcatgaaatt
     1081 gtgattgtcg aacatggcac gggtattcat gtgtttaatg ggcagcccta taccatcacc
     1141 ggtggcacgg tctgtttcgt acgcgatcat gatcggcatc tgtatgaaca taccgataat
     1201 ctgtgtctga ccaatgtgct gtatcgctcg ccggatcgat ttcagtttct cgccgggctg
     1261 aatcagttgc tgccacaaga gctggatggg cagtatccgt ctcactggcg cgttaaccac
     1321 agcgtattgc agcaggtgcg acagctggtt gcacagatgg aacagcagga aggggaaaat
     1381 gatttaccct cgaccgccag tcgcgagatc ttgtttatgc aattactgct cttgctgcgt
     1441 aaaagcagtt tgcaggagaa cctggaaaac agcgcatcac gtctcaactt gcttctggcc
     1501 tggctggagg accattttgc cgatgaggtg aattgggatg ccgtggcgga tcaattttct
     1561 ctttcactgc gtacgctaca tcggcagctt aagcagcaaa cgggactgac gcctcagcga
     1621 tacctgaacc gcctgcgact gatgaaagcc cgacatctgc tacgccacag cgaggccagc
     1681 gttactgaca tcgcctatcg ctgtggattc agcgacagta accacttttc gacgcttttt
     1741 cgccgagagt ttaactggtc accgcgtgat attcgccagg gacgggatgg ctttctgcaa
     1801 taatcttggg ggaaattaag accaaactcg atgacctcca aaaagatgta acttctctta
     1861 agatcgatat ggcaacggtg aaaaccgagt tatctgcggt caggatggag ataggtacag
     1921 tcaaggatga tgttaaagat gtcaaagggc gggctaatgc tcaaatttgg gcgttgattc
     1981 ttgccgtcat cggagccata attaccacct tggtgcgttt tggcattttc cctaatccct
     2041 aacaaaaaag cgaccaggct tttctttcaa ttgcccgatc gcctttgata ttttcccaaa
     2101 ggataaaagc tagtccattc agaatcgagc cttaaagtac tcccatattg gctagcccca
     2161 gaattactcc agcgccgagg atgtggccaa agctagcggt gcccagcaca gcccctaaac
     2221 caaagccgcc aaagaagtta gaggaaggca tgggggtgcc cacattttgt tgtttgatgg
     2281 tcaatttacc aaaggcgatc gccaaaatgt tgcaagcaat catcacccca gcaactttag
     2341 ggctccagga cagggtggcg ggaacggcgg tggccaacaa aaagctatgc attgagattc
     2401 tccagaataa agacggtttt taaagggata gccccacgct aatgggggtc tttaaaaatc
     2461 tcatcttacg gggacgctct gcccctggga aaccaccgtt gcaatactta acaaattttc
     2521 gtttttagct tggcaaatgt ctttggcaaa attggttgat ctggcttaaa tcgtcagtta
     2581 tttgccctgg aatagtctgg ggacgggcaa ttctgatcag atttaccccc aacgcttccg
     2641 ccactttttg cttaaccaat tctcccccct gggcaccgga ggctttagtt accacccctt
     2701 gaatttgcca ttgttgccac agggcttttt ccaatggttc ggctacgggg gggcgcaaag
     2761 caatgatacg gtcggaagta aacccagcgg cgatcgcctg ggctagggct tggggatagg
     2821 gcagaatacg ggcaaataga acaaatctgg agccggtgag cgtgggtctc gcggtatcat
     2881 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2941 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3001 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3061 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3121 ttaacgtgag ttttcgttcc actgagcgtc agacccctta ataagatgat cttcttgaga
     3181 tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg
     3241 tttttcgaag gttctctgag ctaccaactc tttgaaccga ggtaactggc ttggaggagc
     3301 gcagtcacca aaacttgtcc tttcagttta gccttaaccg gcgcatgact tcaagactaa
     3361 ctcctctaaa tcaattacca gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt
     3421 tggactcaag acgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt
     3481 gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga
     3541 gacaaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag
     3601 cgcacgaggg agccgccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3661 caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa
     3721 aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga
     3781 aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg
     3841 agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg
     3901 cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata
     3961 gtaagccagt atacactccg ctagcgctga ggtctgcctc gtgaagaagg tgttgctgac
     4021 tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc acggttgatg
     4081 agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc cacggaacgg
     4141 tctgcgttgt cgggaagatg cggtgggtca atccctcggt ttggcgatcg ccttggtgtt
     4201 tgtcattgct ggtattgcct ttaaaatttc cgctgtaccc ttccaccaat ggacccccga
     4261 cgtttatgaa ggttctccca ccccggtggt ggcttttctt tcggtgggct ctaaggcggc
     4321 cggttttgcc gtagctatcc gattactagt gactgccttt ggtggtatta ccgatgagtg
     4381 gcacgttatt ttcaccgctc tggccgtgtt gagcatggtg ctgggcaacg tggtggcttt
     4441 agcccaaacc agcatgaaac ggatgttggc ctactcttcc atcggtcaag caggctttgt
     4501 gatgattggc ctagtggccg gcagtgaaga tggttacgcc agcatggttt tctacatgct
     4561 catctatctg tttatgaacc tgggggcgtt tagttgcatt attctcttca ccctccgcac
     4621 tggcagtgac caaattagtg attacgctgg tctgtaccac aaagacccct tgttaacctt
     4681 gggcttgagc atttgtttat tatccttggg gggcattcct cctctggcgg gctttttcgg
     4741 caaaatttac atcttctggg ccggttggca atcgggattg tatggcctag tcctacttgg
     4801 tctggttacc agtgtagttt ccatctacta ctacatccgg gtggtgaaaa tgatggtggt
     4861 gaaggagccc caggaaatgt ccgaagtaat caaaaattac ccggccatca aatggaattt
     4921 acccggcatg cgtcccctac aggtgggcat tgtcgctact ttggttgcta cctcgctggc
     4981 aggtattctg gctaatcccc tctttaacct cgccaccgat tccgtggtca gcaccaagat
     5041 gttgcagaca gccctccagc aaacaggaga aactccggcg atcgccattt cccatgattt
     5101 accctagggg tatcaggaaa tattgctttg caggcaaaag ccaatgagtg taactataga
     5161 aaccgattta aaggagactg cagacaattt tcgaaaaaac ccgcttcggc gggttttttt
     5221 atagctaaaa ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg
     5281 ccaatggccc attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact
     5341 ggtcgtaatg aatcgggtaa gtttataata tacaaaggag gtagaaatgg tgagcaaggg
     5401 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     5461 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     5521 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     5581 cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc acgacttctt
     5641 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     5701 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     5761 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     5821 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     5881 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     5941 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     6001 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     6061 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaa
//
