LOCUS       pGD283\-\pGGZ001        5816 bp    DNA     circular     14-FEB-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-12160305|
COMMENT     VNTDBDATE|-12160305|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pGD283 - pGGZ001::35S:XVE::A-G|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     CDS             complement(923..1711)
                     /vntifkey="4"
                     /label=ORF\frame\2
     gene            complement(923..1711)
                     /gene="spect (variant)"
                     /vntifkey="60"
     rep_origin      complement(149..768)
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_feature    5763..5786
                     /vntifkey="21"
                     /label=Adapter
     misc_feature    5062..5111
                     /vntifkey="21"
                     /label=dummy
     terminator      5118..5759
                     /vntifkey="43"
                     /label=rbcS
                     /note="terminator and polyadenylation signal from the pea rbcS-E9 gene"
     misc_feature    3609..3612
                     /vntifkey="21"
                     /label=C\Überhang
     CDS             3613..5061
                     /vntifkey="4"
                     /label=XVE\chimäres\Protein
     misc_feature    3552..3607
                     /vntifkey="21"
                     /label=TMV\Omega
                     /note="translational enhancer from the tobacco mosaic virus 5'-leader sequence (Gallie et al., 1988)"
     promoter        3179..3524
                     /vntifkey="30"
                     /label=CaMV\35S\promoter
                     /note="strong constitutive promoter from cauliflower mosaic virus"
     primer_bind     3467..3487
                     /vntifkey="28"
                     /label=35S\promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    5787..5816
                     /vntifkey="21"
                     /label=Methylated\H-A\linker
BASE COUNT     1528 a      1443 c      1380 g      1465 t 
ORIGIN
        1 gtattctaga ccaagcatgc acagctggcg ttctcagcca gatccccttg acaggatata 
       61 ttggcgggta aactaagtcg ctgtatgtgt ttgtttgaga tctcatgtga gcaaaaggcc 
      121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
      181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
      241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
      301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
      361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
      421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
      481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
      541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
      601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga agaagagttg 
      661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
      721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
      781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
      841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
      901 atgtgtaaca ttggtctagt gattatttgc cgactacctt ggtgatctcg cctttcacgt 
      961 agtgaacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tgtccaagat 
     1021 aagcctgcct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc 
     1081 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg 
     1141 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg 
     1201 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct 
     1261 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca 
     1321 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt 
     1381 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa 
     1441 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca 
     1501 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca 
     1561 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta 
     1621 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag 
     1681 tcgatacttc ggcgatcacc gcttccctca taacacccct tgtattactg tttatgtaag 
     1741 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat 
     1801 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga 
     1861 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca 
     1921 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg 
     1981 atggggcgat tcaggcgatc cccatccaac agcccgccgt cgagcgggct tttttatccc 
     2041 cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc cgcaaagcct 
     2101 tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg ctaatcaggg 
     2161 tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg aaatcgctaa 
     2221 tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc aaacacccct 
     2281 cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat tatgaatata 
     2341 tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac cggctccgcc 
     2401 cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac ccggcggccg 
     2461 gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc gaaaggcggc 
     2521 aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg atatattgtg 
     2581 gtgtaacgtt ctcgaactag gctcggacga agtaagcggc cgcaacctta attaaacacg 
     2641 gcgcgccaac agcgatcgca ccaggtacca cctccgatcc attcggtccc cagattagcc 
     2701 ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac gcagcacgtc 
     2761 tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt cccaagaagg 
     2821 ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat 
     2881 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cacaaaccaa 
     2941 ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca aaggccatgg 
     3001 agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca 
     3061 tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg 
     3121 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg 
     3181 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct 
     3241 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg 
     3301 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc 
     3361 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg 
     3421 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag 
     3481 acccttcctc tatataagga agttcatttc atttggagag aacacggggg acgagctaac 
     3541 atatttttac aacaattacc aacaacaaca aacaacaaac aacattacaa ttactattta 
     3601 caattacagg ctatgaaagc gttaacggcc aggcaacaag aggtgtttga tctcatccgt 
     3661 gatcacatca gccagacagg tatgccgccg acgcgtgcgg aaatcgcgca gcgtttgggg 
     3721 ttccgttccc caaacgcggc tgaagaacat ctgaaggcgc tggcacgcaa aggcgttatt 
     3781 gaaattgttt ccggcgcatc acgcgggatt cgtctgttgc aggaagagga agaagggttg 
     3841 ccgctggtag gtcgtgtggc tgccggtgaa ccgtcgagcg cccccccgac cgatgtcagc 
     3901 ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc cgacgcgcta 
     3961 gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg atttaccccc 
     4021 cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga gcagatgttt 
     4081 accgatgccc ttggaattga cgagtacggt ggggatccgt ctgctggaga catgagagct 
     4141 gccaaccttt ggccaagccc gctcatgatc aaacgctcta agaagaacag cctggccttg 
     4201 tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc catactctat 
     4261 tccgagtatg atcctaccag acccttcagt gaagcttcga tgatgggctt actgaccaac 
     4321 ctggcagaca gggagctggt tcacatgatc aactgggcga agagggtgcc aggctttgtg 
     4381 gatttgaccc tccatgatca ggtccacctt ctagaatgtg cctggctaga gatcctgatg 
     4441 attggactcg tctggcgctc catggagcac ccagggaagc tactgtttgc tcctaacttg 
     4501 ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg tggagatctt cgacatgctg 
     4561 ctggctacat catctcggtt ccgcatgatg aatctgcagg gagaggagtt tgtgtgcctc 
     4621 aaatctatta ttttgcttaa ttctggagtg tacacatttc tgtccagcac cctgaagtct 
     4681 ctggaagaga aggaccatat ccaccgagtc ctggacaaga tcacagacac tttgatccac 
     4741 ctgatggcca aggcaggcct gaccctgcag cagcagcacc agcggctggc ccagctcctc 
     4801 ctcatcctct cccacatcag gcacatgagt aacaaaggca tggagcatct gtacagcatg 
     4861 aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg agatgctgga cgcccaccgc 
     4921 ctacatgcgc ccactagccg tggaggggca tccgtggagg agacggacca aagccacttg 
     4981 gccactgcgg gctctacttc atcgcattcc ttgcaaaagt attacatcac gggggaggca 
     5041 gagggtttcc ctgccacagt ctcaggtgga tcctagataa cctttacctt cattccctgt 
     5101 aaattggtac cctgcaaagc tttcgttcgt atcatcggtt tcgacaacgt tcgtcaagtt 
     5161 caatgcatca gtttcattgc gcacacacca gaatcctact gagtttgagt attatggcat 
     5221 tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg ttttttattc 
     5281 ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg aatgatatgg 
     5341 tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt tgtgtgttga 
     5401 atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg 
     5461 gcctctaatg accgaagtta atatgaggag taaaacactt gtagttgtac cattatgctt 
     5521 attcactagg caacaaatat attttcagac ctagaaaagc tgcaaatgtt actgaataca 
     5581 agtatgtcct cttgtgtttt agacatttat gaactttcct ttatgtaatt ttccagaatc 
     5641 cttgtcagat tctaatcatt gctttataat tatagttata ctcatggatt tgtagttgag 
     5701 tatgaaaata ttttttaatg cattttatga cttgccaatt gattgacaac atgcatcaat 
     5761 cgactatgag taggcaagat gttctgtagg accttgagac cgaaaaggtg gtctca 
//
